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Citations for Takara Bio :
2601 - 2650 of 3947 citations for hsa let 7f RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... was reverse-transcribed to cDNA using random hexamers and oligo (dT) by following the instructions provided in the Prime-Script RT Reagent Kit (Perfect Real Time from TaKaRa Bio Inc., Otsu Shiga, Japan). RT-qPCR was carried out in a StepOnePlus Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Zoology 2021Quote: ... The reverse transcription of 1 μg of total RNA was performed using PrimeScript® RT reagent kit with gDNA Eraser (RR047A, Takara Bio Inc., Dalian, China). The gene expression was determined through RT-PCR ...
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bioRxiv - Biochemistry 2022Quote: ... Total RNA was isolated using RNAqueous isolation kit (Thermo-Fisher, Waltham MA) and mRNA reverse transcribed using PrimeScript RT Reagent Kit (Takara Bio USA, Mountain View CA). Quantitative PCR reactions were carried out using PowerUp SYBR-Green master mix using StepOne Real-Time PCR systems (Applied Biosystems) ...
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bioRxiv - Plant Biology 2020Quote: ... the HSP17.6C cDNA was amplified by PCR using PrimeSTAR GXL DNA polymerase (Takara) and gene-specific primer sets ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesized using a combination of SMARTer PCR cDNA Synthesis Kit (Takara) and SuperScript II Reverse Transcriptase (ThermoFisher) ...
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bioRxiv - Cancer Biology 2021Quote: ... qRT-PCR was performed using SYBR® Premix Ex Taq™ (Takara, Japan) and 300 nmol/L of each primer ...
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bioRxiv - Immunology 2021Quote: ... Real-time PCR was performed using SYBR Premix Ex Taq II (Takara Bio). The primers used in the assay were as follows ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The cDNA was synthesized using SMARTer® PCR cDNA Synthesis Kit (Clontech, USA) which was then amplified using the KAPA HiFi PCR Kits (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... Real-time PCR was performed using the SYBR pPCR premix Ex Taq (Takara) or LUNA Universal qPCR Master Mix (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... 10μL of eluate were used as a template for PCR with KOD (Takara) with extension time 1’10’’ ...
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bioRxiv - Bioengineering 2022Quote: ... PCR products were ligated to pMD18-T cloning vector (Takara Bio, Beijing, China) and then transformed in E.coli ...
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bioRxiv - Genomics 2020Quote: ... cDNA was synthesized using the SMART PCR cDNA Synthesis Kit (Clontech, CA, USA), and then fractionized with BluePippin® (Sage Science ...
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bioRxiv - Molecular Biology 2021Quote: ... Mutations were introduced by whole-plasmid PCR using PrimeSTAR MAX DNA polymerase (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2019Quote: ... The resulting progeny was genotyped using EmeraldAmp GT PCR Master Mix (Takara, RR310) and primers flanking the deleted region of Abhd2 ...
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bioRxiv - Genetics 2019Quote: ... The bisulfite-treated DNA was PCR-amplified with TaKaRa EpiTaq HS (TaKaRa Bio) with primers specific for chimpanzee and rhesus macaque CGIs (Supplementary Table 4) ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were amplified with PrimeSTAR HS DNA polymerase (Clontech Laboratories, Inc., USA). DNA isolations and manipulations were carried out using standard protocols ...
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bioRxiv - Zoology 2019Quote: ... Polymerase chain reactions (PCRs) were performed using Tks Gflex™ DNA Polymerase (Takara) and conducted in a T100™ Thermal Cycler (Bio-Rad) ...
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bioRxiv - Genetics 2019Quote: ... the PCR fragment was cloned into a vector using an Infusion kit (Takara), and the linear construct was amplified with T7/T3 primer set from the plasmid template ...
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bioRxiv - Bioengineering 2019Quote: ... and cDNA was subjected to real-time PCR using SYBR green mix (TaKaRa). PCR cycles were performed on an ABI 7500 PCR System ...
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bioRxiv - Systems Biology 2020Quote: The PCR reaction was carried out using PrimeSTAR HS DNA Polymerase (Takara, Japan) following the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2020Quote: ... and the PCR products recovered were connected with pMD-19T carrier(Takara, Janpan), then converted to DH5 alpha receptive cells (Vazyme ...
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bioRxiv - Biochemistry 2020Quote: Constructs for mammalian cell experiments were cloned using backbone PCR and inFusion (Clontech). Monobody HA4 or OptoMB variants were PCR amplified and Gibson-assembled from bacterial plasmids (described above ...
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bioRxiv - Neuroscience 2021Quote: ... PCR was performed using the TB Green Ex Taq II Mix (Takara Bio) and the Thermal Cycler Dice Real Time System (Takara Bio ...
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bioRxiv - Cell Biology 2021Quote: ... for lem2+ was amplified from genomic DNA using HiFi PCR master mix (Clontech) and cloned into NdeI/XhoI-digested pREP41-MCS+ ...
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bioRxiv - Plant Biology 2021Quote: ... The PCR product was cloned directly into pMD20-T vector (Takara, Beijing, China), which was then transformed into competent E ...
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bioRxiv - Microbiology 2020Quote: ... qRT-PCR was performed with SYBR Premix Ex Taq II (Takara, Dalian, China) using Mx3000P (Stratagene ...
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bioRxiv - Plant Biology 2021Quote: ... Real-time PCR was performed using a SYBR premix kit (TaKaRa, Cat#RR82LR). The primers used are listed in Supplemental Table 3.
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bioRxiv - Neuroscience 2020Quote: ... Reverse transcription PCR was performed in a thermal cycler (Takara Shuzo, Tokyo, Japan) using 1 x cycle of the following programme ...
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bioRxiv - Genomics 2021Quote: LR-PCR amplification reaction was performed using PrimeSTAR GXL DNA Polymerase kit (Takara) according to manufacturer”s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Quantitative real-time PCR was carried out using SYBR Premix Ex Taq (Takara Bio Incorporated ...
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bioRxiv - Plant Biology 2021Quote: ... Each PCR was conducted using TaKaRa LA Taq® with GC buffer (TaKaRa), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... PCR amplification was conducted using TaKaRa LA Taq® with GC buffer (TaKaRa), gene specific primers (300 nM of each) ...
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bioRxiv - Microbiology 2021Quote: ... fragments were PCR amplified from the plasmids using PrimeSTAR GXL Polymerase (Takara, Japan) and Nhel_IDT_F and EcoRI_ITD_R primers (Supplementary table 7) ...
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bioRxiv - Molecular Biology 2021Quote: ... OLFM4 homology regions were amplified with CloneAmp™ HiFi PCR Premix (Takara 639298) using primers KB39+KB40 and KB69+KB38 (Supplemental Table 3) ...
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bioRxiv - Cancer Biology 2020Quote: ... and real-time PCR was carried out using the SyBr green mix (Takara) in a real-time PCR machine (Biorad CFX96 Real-Time System) ...
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bioRxiv - Cell Biology 2021Quote: ... Quantitative real time-PCR was performed using TB Green Premix Ex Taq (Takara) in QuantStudio 7 Flex Real-Time PCR System (Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... PCR reactions were performed using TaKaRa LA Taq Hot Start polymerase kit (TaKaRa) and 50ng of total genomic DNA in a 50uL PCR reaction ...
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bioRxiv - Cell Biology 2020Quote: ... and the ACP were amplified by PCR with PrimeSTAR Max DNA Polymerase (Takara). The primers for PCR were listed in the S1 Table ...
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bioRxiv - Plant Biology 2022Quote: ... qRT-PCR was carried out with SYBR Premix Ex Taq II Premix (Takara) on the real-time system (Roche ...
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Effects of Sanhuang plaster on expression of MyD88, TRAF6, MIP-1β and IL-23 in rats infected by MRSAbioRxiv - Molecular Biology 2022Quote: ... Real-time fluorescence quantitative PCR kit and reverse transcription kit(TaKaRa Co., Ltd.); MyD88 Anti-body TRAF6 Anti-body MIP-1β Anti-body and IL-23 Anti-body (GeneTexCo. ...
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bioRxiv - Biochemistry 2022Quote: ... qRT-PCR was performed with SYBR Premix Ex TaqII (RR820A, Takara Bio Inc.) in an ABI 7300 thermal cycler (Applied Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... and amplified genomic DNA by PCR using the PrimeSTAR HS DNA Polymerase (TaKaRa) or KOD-plus Ver ...
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bioRxiv - Cancer Biology 2022Quote: ... and gel extracted using the NucleoSpin Gel and PCR Clean-up kit (Takara Bio USA Inc ...
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bioRxiv - Developmental Biology 2022Quote: ... Quantitative PCR was performed using a TB Green Premix DimerEraser Kit (RR091A; Takara). The primer sequences are listed in Supplementary Table S1.
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TIR signaling promotes the interactions between EDS1/PAD4 and ADR1-L1 and oligomerization of ADR1-L1bioRxiv - Plant Biology 2021Quote: ... Real-time PCR was performed using a SYBR premix kit (TaKaRa, Cat#RR82LR). The primers used are listed in Supplemental Table 1.
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bioRxiv - Microbiology 2021Quote: ... PCR products were cloned into pGE450 using the In-Fusion cloning system (Takara). Start codon mutant constructs were made by inverse PCR-amplification of the wild-type constructs using primers that introduce a start codon mutation (“T” to “C” change at the central position of the start codon ...
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bioRxiv - Molecular Biology 2019Quote: LncRNA expression was measured using the SYBR® Green PCR Kit (Takara, Japan) in a blinded fashion ...
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bioRxiv - Microbiology 2021Quote: ... Real-time PCR was performed using SYBR Premix Ex Taq II (TaKaRa, RR820A) on LightCycler96 qPCR system (Roche) ...
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bioRxiv - Microbiology 2021Quote: ... The amplicon was purified by NucleoSpin® Gel and PCR Clean-Up (Takara) for Sanger sequencing.
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bioRxiv - Biophysics 2020Quote: ... PCR-based site-directed mutagenesis with In-Fusion HD Cloning system (Takara, Clonetech) was used to generate PscD D43A ...