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Citations for Takara Bio :
201 - 250 of 1281 citations for ETS Related Transcription Factor Elf 3 ELF3 Antibody HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: Reverse transcription was performed using Primescript II 1st strand cDNA synthesis kit (TaKaRa, Japan) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... the reverse transcription reactions were performed using reverse Transcriptase M-MLV (RNase H-) (TaKaRa). The cDNA was then used for real-time qPCR using the SYBR Green qPCR Master Mix (Bi-make ...
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bioRxiv - Neuroscience 2020Quote: ... Guide-it In Vitro Transcription and Screening Kit (Takara Bio USA, Mountain View, California) was used ...
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bioRxiv - Microbiology 2021Quote: Reverse transcription and quantitative PCR reactions were performed using PrimeScript RT reagent Kit (Takara) and SYBR Premix Ex Taq II (Takara ...
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bioRxiv - Neuroscience 2022Quote: ... Reverse transcription was performed using PrimeScript II 1st strand cDNA Synthesis Kit (Takara, Japan) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... Reverse transcription was performed with 1 μg RNA using PrimeScript RT reagent Kit (Takara). The cDNA was amplified with gene-specific primers (Supplemental Table 1 ...
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bioRxiv - Microbiology 2020Quote: ... and submitted to reverse transcription-quantitative PCR with the PrimeScript RT reagent kit (TaKaRa). The primers to detect Zika-specific RNA were listed below ...
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bioRxiv - Cell Biology 2020Quote: ... reverse transcription was carried out using 50 U/well SmartScribe (SmS) RT enzyme (Takara) + 1x SmS First Strand buffer (Takara ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Complementary DNA were synthesized by reverse transcription using PrimeScriptTMRT Master Mix (TaKaRa, Dalian, China). qPCR was achieved on a Roche LC96 Real-Time PCR Detection System with 2 × T5 Fast qPCR Mix (Tsingke Biotechnology ...
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bioRxiv - Cell Biology 2022Quote: ... The reverse transcription reaction was performed using ExScript RT Reagent Kit (Takara, Tokyo, Japan). The primers used in this study were as follows:
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bioRxiv - Plant Biology 2021Quote: ... Transcription of corresponding genes and ACTIN7 was analysed using SYBR Premix Ex Taq (TAKARA) with a BIO-RAD CFX Connect Real-Time System ...
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bioRxiv - Microbiology 2021Quote: ... The reverse transcription of RNA into cDNA using PrimeScript RT reagent kit (TaKaRa, China). Subsequently ...
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bioRxiv - Zoology 2020Quote: ... the in vitro Transcription T7 Kit (for siRNA Synthesis) (Takara Bio Inc., Shiga, Japan) was used.
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... followed by reverse transcription to synthesize cDNA using PrimeScript RT kit from Clontech (RR037A). qPCR was performed using a two-step PCR protocol (melting temperature ...
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bioRxiv - Cell Biology 2020Quote: ... Reverse transcription was performed using PrimeScript RT Master Mix (CD201-2, TaKaRa, Otsu, Japan), and qPCR was performed using SYBR Premix Ex Taq II (RR820L ...
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bioRxiv - Immunology 2020Quote: ... The cDNA templates were synthesized using the RNA reverse transcription kit (Takara, Tokyo, Japan). Quantitative PCR was performed using TB Green® Premix Ex Taq™ II (Takara ...
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bioRxiv - Microbiology 2020Quote: ... were subjected to reverse transcription with oligo dT primer (using Takara cat#RR037A Kit), and then the corresponding cDNAs were quantified using Hieff qPCR SYBR Green Master Mix (Yeason) ...
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bioRxiv - Immunology 2021Quote: ... The RNA of the SARS-CoV-2 was extracted for reverse transcription (TAKARA, Japan). The SARS-CoV-2 was quantitative analyzed with real time PCR by targeting S protein.
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bioRxiv - Neuroscience 2022Quote: ... The reverse transcription products (cDNA) were amplified using PrimeSTAR DNA polymerase (TaKaRa, Shiga, Japan) and specific primers ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was synthesized by reverse transcription using the Prime Script RT reagent kit (TaKaRa) and subjected to quantitative RT-PCR with Setd2 and target gene primers in the presence of the SYBR Green Realtime PCR Master Mix (Thermo) ...
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bioRxiv - Cancer Biology 2024Quote: ... Reverse transcription of total RNA was performed using a two-step kit (TaKaRa, Japan), and real-time qPCR experiments were conducted using the SYBR fluorescent dye kit (TaKaRa ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA was prepared from total RNA by reverse transcription using oligo-dT primers (Takara). RT-qPCR was conducted using SoFast EvaGreen Super Mix (Bio-Rad ...
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bioRxiv - Plant Biology 2023Quote: ... 32P-labeled dsRNAs were synthesized using the in vitro T7 Transcription kit (Takara Bio) with [α-32P]-UTP (PerkinElmer ...
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bioRxiv - Plant Biology 2022Quote: ... First-strand cDNA was synthesized by reverse transcription using PrimeScript RT Master Mix (Takara Bio Group ...
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bioRxiv - Plant Biology 2022Quote: Reverse transcription was conducted using PrimeScript RT Reagent Kit with gDNA Eraser (Takara, Japan). Real-time PCR was carried out using a KAPA SYBR FAST qPCR Master Mix 2X (Kapa Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... followed by reverse transcription using PrimeScript™ RT Master Mix (Takara, cat. no. RR036A) per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... gDNA digestion and reverse transcription were performed with PrimeScript RT Reagent Kit (Takara RR047B). qPCR for endogenous transcripts ...
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bioRxiv - Genetics 2024Quote: ... and first-strand cDNA was synthesized using the Reverse Transcription Kit (Takara, Dalian, China). Primers for the qRT-PCR assay targeting the TaNADPO gene were designed (Supplemental Table 13) ...
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bioRxiv - Molecular Biology 2024Quote: Reverse transcription of total RNA was performed using the PrimeScript RT reagent kit (Takara). Quantitative real-time PCR was performed using TB Green Premix Ex Taq II (Takara ...
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bioRxiv - Cell Biology 2024Quote: ... Reverse transcription was performed using PrimeScript RT master mix (TaKaRa Bio Inc., Shiga, Japan) with a T100™ Thermal Cycler (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2024Quote: ... Reverse transcription was done using the PrimeScript™ RT reagent Kit (Takara Bio, USA) according to the manufacturer’s recommendation ...
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bioRxiv - Biochemistry 2024Quote: ... The sequence of human histone H2A(K15C-129) was cloned into the pCold-Trigger factor (TF) vector (Takara Bio) with a SUMO coding sequence inserted to generate His6–TF–SUMO–H2A(K15C-129 ...
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bioRxiv - Cancer Biology 2022Quote: ... 3’ multiplexed RNA-sequencing was performed with the Takara SMART-Seq v4 3’ DE Kit (Takara 635040) followed by Nextera XT (Illumina FC-131-1024 ...
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bioRxiv - Microbiology 2021Quote: ... complementary to the DENV 3’UTR at 65°C for 3 hrs using ExpressHyb hybridization buffer (Clontech). Autoradiographs were quantified using ImageQuant (GE Healthcare).
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bioRxiv - Immunology 2021Quote: ... immunoreactive bands were visualized using Western BLoT Quant HRP Substrate (Takara Bio) or Western BLoT Ultra Sensitive HRP Substrate (Takara Bio).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The membrane was incubated with Western BLoT Chemiluminescence HRP Substrate (TaKaRa Bio), and membrane image was captured using a chemiluminescent imaging system LuminoGraph I (ATTO ...
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bioRxiv - Microbiology 2023Quote: ... Chemiluminescence was detected using Western BLoT Ultra Sensitive HRP Substrate (T7104A, Takara) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2023Quote: ... Expression level of Venus and Venus-tagged arrestin-3 proteins was determined with anti-GFP JL-8 antibody (#632381, Takara Bio USA, San Jose, CA). The endogenous β-actin (loading control ...
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bioRxiv - Cell Biology 2020Quote: ... behind the myc-tag in the BamHI-digested pCMV-Myc-HIF-1α DM(PP/AA) construct (Berra et al,2003) using In-Fusion HD Cloning (Clontech). Then ...
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bioRxiv - Developmental Biology 2020Quote: The ERK-KTR-mCherry construct for transient expression was prepared by first inserting the coding sequence for ERK-KTR (Regot et al., 2014) into a CMV-driven mCitrine C1 expression vector (TaKaRa), and then replacing the fluorophore for mCherry ...
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bioRxiv - Developmental Biology 2021Quote: DN-Brn2 expression vector was generated by PCR amplification of Brn2 DBD and addition of NES sequences using primers ATC ATC GAA TTC GAG AGT CAT GCT TCA ACT TCC TCC TCT TGA ACG CCT TAC CCT TGG AGG AGG AGG ACC GGG CCA CCC AGG CGC GCA C and GAT GAT GGA TCC CCA AGG GTA AGG CGT TCA AGA GGA GGA AGT TGA AGT CCT CCT CCT CCA CCC CCA TAC ACA TCC TCG GC and mBrn2 template plasmid (Sugitani et al. 2002) into mCherry N1 (Clontech). Subsequently ...
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bioRxiv - Plant Biology 2022Quote: ... Amplified fragments were cloned into the TRV vector (pTRV2) (Lu et al., 2003) using the In-Fusion HD cloning kit (Clontech). Agrobacterium strains containing desired pTRV2 constructs were co-infiltrated with strains carrying pTRV1 at OD600=0.5 into N ...
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bioRxiv - Molecular Biology 2020Quote: ... To generate the light-inducible clustering constructs, the CRY2olig sequence (Taslimi et al., 2014a) was inserted with a c-terminal mCherry tag (Clontech) into pGEMHE to generate pGEMHE-CRY2olig-mCherry ...
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bioRxiv - Cell Biology 2021Quote: ... cells were stably transfected with a plasmid encoding human full-length keratin 8 with an EYFP tag at its carboxyterminus (Windoffer et al., 2004; recloned into pEYFP-N1 (Clontech) with BamHI and EcoRI) ...
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bioRxiv - Cell Biology 2020Quote: ... The plasmid pJYB240 was generated from pJYB212 by exchanging the mEos2 open reading frame by a PCR-amplified mTurquoise2 DNA (Goedhart et al. 2012) using the InFusion kit (Clontech). The parBF-mTurquoise2 gene from pJYB240 was introduced into pJYB243 ...
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bioRxiv - Plant Biology 2020Quote: To test the interaction of UVR8 with RUP2, RUP2 was cloned into pGBKT7-GW (Lu et al., 2010) and transformed into the Y2H Gold strain (Clontech). UVR8 and UVR8G101S were introduced into pGADT7-GW (Marrocco et al. ...
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bioRxiv - Plant Biology 2021Quote: Yeast two-hybrid assays were performed as described (Cesari et al., 2013) using the Matchmaker Gold Yeast Two-hybrid system (Clontech).
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bioRxiv - Neuroscience 2020Quote: ... the 11kb genomic region encompassing kdm5 was amplified by PCR from w1118 and cloned into the pattB vector (Bischof et al., 2007) using the In-Fusion cloning system (Takara). A 3xHA tag was included in-frame at the 3’end of the kdm5 ORF ...
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bioRxiv - Cell Biology 2020Quote: ... GFP CIZ1 C275 was made by ligating the 1 kb C-terminal XhoI fragment (Coverley et al., 2005) into the XhoI site of pEGFP-C2 (Clontech). GFP CIZ1 Δp8 (this study ...
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bioRxiv - Developmental Biology 2022Quote: ... RNAs were generated using a SMART-seq method that combined parts of the Smart-seq2 (Picelli et al., 2014) and SMART-Seq v4 (Takara) protocols ...