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Citations for Takara Bio :
251 - 300 of 1281 citations for ETS Related Transcription Factor Elf 3 ELF3 Antibody HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... The protein-coding genomic sequence including 3 kbp promoter sequence was PCR-amplified and cloned into the pGreen0029 binary vector (Hellens et al., 2000) using In-Fusion Cloning system (Takara Bio Europe ...
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KIF24 controls the clustering of supernumerary centrosomes in pancreatic ductal adenocarcinoma cellsbioRxiv - Cell Biology 2022Quote: ... human KIF24 fragments encoding residue 1-4383 was excised from pEGFP-C1-KIF24 (Kobayashi et al., 2011) and sub-cloned into pLVX-IRES-Puro (Clontech). KIF24/TS621-622AA (TS ...
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bioRxiv - Plant Biology 2021Quote: ... the promoter and the 2,868-bp genomic fragment from the MCR start codon to 798 bp downstream of the stop codon were separately amplified from Col-0 genomic DNA and cloned into SacI/NcoI sites in the pGR111 binary vector (Dobritsa et al., 2010) through In-Fusion cloning (Takara). An AgeI site was introduced in front of the MCR start codon for ease of subsequent cloning ...
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Integrated requirement of non-specific and sequence-specific DNA binding in MYC-driven transcriptionbioRxiv - Molecular Biology 2020Quote: ... The resulting mutant cDNA and that encoding the Arg 364/366/367 to Ala mutant (Amati et al., 1992) (here MycRA) were subcloned in the retroviral vector pQCXIH (Clontech) or in the transfection vector pCMV-FLAG ...
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bioRxiv - Neuroscience 2021Quote: ... hALG-2 E47A-E114A cDNA was kindly provided by Masatoshi Maki (Shibata et al., 2004) and was subcloned into a pEGFP-C1 vector (Clontech) to obtain GFP-hALG-2 E47A-E114A (GFP-ALG-2ΔCa).
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bioRxiv - Neuroscience 2022Quote: The genomic location of the insertion in the enhancer-trap line Et(gata2:EGFP)bi105 was mapped using a linker-mediated approach with the Universal Genome Walker™ 2.0 Kit (Clontech). A detailed user manual is available online ...
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bioRxiv - Cell Biology 2020Quote: ... the sequence of NAGTI-GFP (N-acetylglucosaminyltransferase I fused to GFP; (Shima et al., 1997)) was cloned into pLVX-TetOne-Puro (Clontech). The constructed plasmid was then co-transfected with psPAX and pVSVG into HEK293T cells to produce lenti-viruses ...
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bioRxiv - Cell Biology 2020Quote: ... we first inserted the mAID sequence after 3xFlag in pCRISPaint-3xFlag-puro (Schmid-Burgk et al., 2016) using the In-Fusion HD Cloning kit (Takara) to generate pCRISPaint-3xFlag-mAID-puro ...
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bioRxiv - Cell Biology 2021Quote: The GFP-Snx9 construct was generated by cloning the cDNA for Snx9 (Hicks et al., 2015) into the HindIII and EcoR1 sites of pEGFP-C3 (Clontech). The mammalian Trbo-GBP construct was generated by cloning a gene synthesize fragment containing two tandem copies of the GFP nanobody into the V5-TurboID-NES_pCDNA3 vector ...
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bioRxiv - Neuroscience 2021Quote: ... together with the P2A (Donnelly et al., 2001) sequence ATNFSLLKQAGDVEENPGP into the modified pEGFP-C1 vector by replacing EGFP (Clontech).
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bioRxiv - Developmental Biology 2021Quote: ... and cloned into into modified pJet:attB:mCherry vector (Roberts et al., 2014) containing phiC31 attB site and mCherry reporter using In-Fusion HD Cloning Kit (TaKaRa/Clontech) according to the manufacture instructions ...
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bioRxiv - Cell Biology 2021Quote: ... The PfDdi1 HR and synthetic DNA PCR were cloned into BglII and SalI linearized pT2A_FIKK10-cKO vector (kind gift of Dr Moritz Treeck, The Francis Crick Institute) (Davies et al., 2020) in an In-Fusion (ClonTech) reaction to produce the plasmid pT2A_Ddi1_cKO ...
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bioRxiv - Neuroscience 2023Quote: ... GFP-T42EM was created by cloning T42EM (Ding et al., 2006) into the HindIII and BamHI sites of the pEGFP-C1 plasmid (6084-1, Clontech). To verify tau protein expression ...
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bioRxiv - Plant Biology 2023Quote: ... The heat treatment lasted for 10 min for S. cerevisiae strains PJ69-4A (James et al. 1996) and AH109 (Clontech), used for the classical Y2H assays ...
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bioRxiv - Biochemistry 2023Quote: ... into which a PCR-amplified DNA fragment containing the mNeonGreen gene (Shaner et al., 2013) was inserted using the in-Fusion reaction (Clontech). To generate pRS426-CPY(1-50)-ATG15(Δ1-35)-mNeonGreen (YPL073) ...
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bioRxiv - Biochemistry 2023Quote: ... 2019) and ACOT8 (ACOT8 H78A) (Ishizuka et al., 2004) were constructed by PCR-mediated mutagenesis using PrimerSTAR DNA polymerase (Takara). cDNAs for proteins expression were constructed in pLV cs2.0 vectors ...
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bioRxiv - Plant Biology 2023Quote: ... and was cloned into the AscI site of the pENTR/D-TOPO entry vector containing full length MpNEK1 CDS (Otani et al., 2018) by In-fusion system (Takara). The resulting vector pENTR/D-TOPO-MpNEK1-mCitrine was subjected to LR reaction to transfer MpNEK1-Citrine fusion into the Gateway binary vector pMpGWB144 (MpEF1α pro:XVE >>LexA operator:Gateway cassette).
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bioRxiv - Plant Biology 2024Quote: ... and introduced into linearized (with KpnI) pCGEN (Motteram et al. 2011) using the In-Fusion HD Cloning Kit (Takara Bio). The resulting vectors ...
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bioRxiv - Plant Biology 2023Quote: ... The fragments of PtBST1 and PtBST2 were cloned into the EcoRI restriction site in pPha-T1/egfp vector (Tachibana et al., 2011) using Mighty Mix (Takara). PtBST3 and PtBST4 fragments were cloned using Gibson assembly system (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... These three amplified fragments were then seamlessly integrated into the Sac I site of YEP352-GAPII (Oka et al. 2007) using the In-Fusion HD Cloning Kit (Takara). Similarly ...
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bioRxiv - Cell Biology 2022Quote: ... The GFP-AktPH construct was obtained from the laboratory of Jason Haugh (North Carolina State University, Raleigh NC) (Haugh et al., 2000) and cloned into the pLVX-IRES-puro vector (Clontech). The GFP-tractin construct was a gift from Dyche Mullins (Addgene plasmid # 58473 ...
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bioRxiv - Biochemistry 2022Quote: A DNA fragment coding HA-tagged Dcp2 was amplified by PCR and cloned into pIExZ vector (Izumi et al, 2020) by In-fusion cloning kit (Takara).
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bioRxiv - Biochemistry 2022Quote: cDNA fragment of Gtsf1 was amplified by RT-PCR from BmN4 total RNAs and cloned into pIExZ vector (Izumi et al, 2020) by In-fusion cloning kit (Takara).
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bioRxiv - Plant Biology 2022Quote: ... pOsSHR2::OsSHR2 or pOsPIN5c::OsPIN5c and inserted into the KpnI digested vector pCam23A (Sun et al., 2016) backbone by using the In-Fusion kit (Clontech), with primers are listed in Supplementary Table 4.
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bioRxiv - Plant Biology 2024Quote: ... the corresponding promoters (ampflied using primer listed in Table S7) were inserted into a modified pUC19 entry vector (Andersen, et al., 2021) via Infusion cloning (Takara) according to the manufacturer description ...
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bioRxiv - Cell Biology 2024Quote: ... pyogenes Cas9-tagBFP expressing HL-60 cells (Graziano et al. (2019)) were transduced by spinoculating the cells on Retronectin-coated (Takara) non-TC treated 6-well plates (Falcon #351146) ...
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bioRxiv - Cell Biology 2024Quote: ... and the resulting double-stranded fragments were subcloned at the BsaI site of the pMpGE_En03 vector (Sugano et al., 2018) using the DNA ligation kit Ver.2.1 (Takara Bio, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... The EF1α cis-element was switched to that of CiNut (Kitaura et al., 2007) with an In-Fusion Cloning Kit (Clontech) according to the previous reports (Sasakura et al. ...
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bioRxiv - Cell Biology 2020Quote: ... from second to third - 5’-ttgaattcgcgctttgtgagcattgc-3’ and 5’-ttgtcgacgttgtcgtccgtgtgcac-3’ and then subcloned into pGAD424 vector (Clontech) in frame with activation domain of GAL4 using restriction sites EcoR1 and Sall.
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bioRxiv - Biophysics 2022Quote: ... 3’-RACE-Ready cDNA template was synthesized using a SMARTer® RACE 5’/3’ Kit (Takara Bio, USA) and subsequently used to amplify 3’ end sequences of R ...
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bioRxiv - Microbiology 2023Quote: The pEGFP-C1 plasmids including AFF4 3’UTR sites “1-2-3” were generated using pEGFP-C1 (Clontech). The following complementary oligonucleotides were annealed in respective pairs as above (1.25 µM each in 75 mM NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... Reverse transcription was performed using PrimeScriptTM II 1st strand cDNA Synthesis Kit (TaKaRa, Tokyo, Japan) with Oligo-T primer to obtain cDNA ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was reversed transcribed to cDNA by the PrimeScript DNA Reverse Transcription kit (Takara, Japan). PCR was conducted by a 7500 Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Immunology 2020Quote: ... Reverse transcription and cDNA preamplification were performed using the SMARTer Ultra Low RNA kit (Clontech) and the Advantage 2 PCR kit according to the manufacturer’s instructions on the C1 device ...
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bioRxiv - Cancer Biology 2020Quote: ... Reverse-transcription was performed using the PrimeScript™ RT Reagent Kit (Perfect Real Time, TAKARA). The A27L (late gene ...
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bioRxiv - Microbiology 2020Quote: ... RNA was transcribed from a DNA fragment using an in vitro Transcription T7 kit (TAKARA).
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bioRxiv - Plant Biology 2021Quote: ... followed by Reverse Transcription with SMART ® MMLV (Takara Bio USA, Mountain view, CA, USA). cDNA was synthesized using Oligo dT from 1 μg of total RNA ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA (1 μg) was reverse transcribed by a reverse transcription kit (RR047A, Takara, China). Real-time quantitative PCR was performed on ABI 7500 system (Applied Biosystems ...
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bioRxiv - Immunology 2022Quote: ... Reverse transcription to cDNA was performed with PrimeScrip RT Master Mix (RR036A, Takara Bio, Japan). The cDNA was used as a template for PCR amplification of the following gene coding sequences (primers shown in Supp ...
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bioRxiv - Zoology 2020Quote: ... Reverse transcription-PCR was conducted using a PrimeScript One-Step RT-PCR kit (Takara, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Reverse transcription (RT) was performed using the PrimeScript RT master mix (cat. #RR036A, Takara, Japan). Real-time RT-PCR was performed in a StepOne plus system (ABI StepOne Plus ...
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bioRxiv - Cancer Biology 2020Quote: ... Reverse transcription was performed using the Takara PrimeScript cDNA Synthesis kit (Takara Bio Europe, France). For cDNA library preparations ...
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bioRxiv - Molecular Biology 2021Quote: RNA reverse transcription was performed using the PrimeScript RT reagent Kit with gDNA Eraser (TaKaRa). The qPCR reaction system included the following ...
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bioRxiv - Molecular Biology 2021Quote: ... Reverse transcription reactions were performed with the PrimeScript RT reagent kit with gDNA Eraser (Takara). Real-time PCRs were performed with a Bio-Rad real-time thermal cycling system using SYBR-Green ...
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bioRxiv - Microbiology 2020Quote: ... The first-strand cDNA was then synthesized using a reverse transcription kit (TaKaRa, Dalian, China) following the manufacturer’s instruction ...
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bioRxiv - Genomics 2020Quote: ... 3μl of reverse transcription (RT) mix was added (0.475μl SmartScribe [Takara], 0.125μl RNase inhibitor [Takara, cat no ...
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bioRxiv - Microbiology 2021Quote: ... The in vitro transcription reaction was performed using T7 RNA polymerase (2540A; Takara Bio, Inc.) in accordance with the manufacturer’s protocol ...
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bioRxiv - Pathology 2022Quote: Reverse transcription PCR (RT-PCR) was performed with 500 ng RNA per reaction by TaKaRa PrimeScriptTM RT Master Mix (Perfect Real Time ...
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bioRxiv - Microbiology 2023Quote: ... reverse transcription was performed by using the PrimeScript RT Kit (Takara Bio, San Jose, CA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was obtained by reverse transcription using the PrimeScript RT Reagent Kit (Takara, Kusatsu, Japan). RT-qPCR was performed with the respective human-specific sense and antisense primers and RT-SYBR™ Green PCR Master Mix (Applied Biosystems) ...