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Citations for Takara Bio :
201 - 250 of 1325 citations for Copper;5 10 15 20 tetrakis 4 methylphenyl porphyrin 22 24 diide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and pVSV-G (#PT3343-5, Clontech) vectors were transfected using Lipofectamine 3000 ...
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bioRxiv - Neuroscience 2024Quote: ... reverse primer (5’-caccgccatggtggcggatcccttgtacagctcgtccatgcc −3’) (Clontech).
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bioRxiv - Neuroscience 2024Quote: ... reverse primer (5’-cacgtctcatcccctccatctttgacctttcaaagtg - 3’) (Clontech). The full-length Tenm4 cDNA ...
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bioRxiv - Neuroscience 2021Quote: ... Lentiviral collection and spindown was performed at 24 hours and 48 hours after initial change of medium with Lenti-X-concentrator (Clontech) according to manufacturer protocol ...
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bioRxiv - Plant Biology 2020Quote: ... and reverse-transcribed with T7-oligo(dT)24 as a primer using the PrimeScript II 1st strand cDNA Synthesis kit (TaKaRa). Then the poly(A ...
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bioRxiv - Microbiology 2022Quote: ... cells were transduced in 24-well plates precoated with recombinant fibronectin (FN CH-296, Retronectin Takara, Clontech, Mountain View, CA) with retroviral supernatants and expanded in complete medium (45% RPMI-1640 and 45% Click’s medium ...
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bioRxiv - Microbiology 2022Quote: ... cells were transduced in 24-well plates precoated with recombinant fibronectin (FN CH-296, Retronectin Takara, Clontech, Mountain View, CA) with retroviral supernatants and expanded in complete medium (45% RPMI-1640 and 45% Click’s medium ...
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bioRxiv - Developmental Biology 2021Quote: ... 24 hour old pluripotent spheroids were subjected to CL media consisting of RHB Basal media (Takara/Clontech, cat. no. Y40000), 5% KSR (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... 24 hour old pluripotent spheroids were subjected to CL media consisting of RHB Basal media (Takara/Clontech, cat. no. Y40000), 5% KSR (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Culture supernatants containing lentivirus were collected 24 and 48 h after transfection and concentrated by a Lenti-X concentrator (Takara) followed by passing through a 0.45-μm PES filter ...
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bioRxiv - Synthetic Biology 2022Quote: ... Media was exchanged after 12 hours and VLPs harvested after 24 and 48 hours and enriched fiftyfold using LentiX concentrator according to the protocol provided by the manufacturer (Takara).
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bioRxiv - Cell Biology 2023Quote: ... cells were seeded as described above and treated for 24 h with 500 nM Rapalog A/C hetero-dimerizer (Takara). Cells were collected as described above ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted from pre-metastatic (0 hrs APF) and post-metastatic (24 hrs APF) Scrib knockdown pupae using TRI reagent (TAKARA) as per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... cells were seeded as described above and treated for 24 h with 500 nM Rapalog A/C hetero-dimerizer (Takara). Cells were collected as described above ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and glyceraldehyde 3-phosphate dehydrogenase (5′-GCACCGTCAAGGCTGAGAAC-3′ and 5′-TGGTGAAGACGCCAGTGGA-3′; Takara Bio Inc., Shiga, Japan) were used for RT-PCR.
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bioRxiv - Microbiology 2024Quote: ... 5′ RACE was carried out using SMARTer RACE 5′/3′ Kit (Takara Bio USA, Mountain View, CA) to identify the transcription start site (TSS ...
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bioRxiv - Developmental Biology 2020Quote: ... and 20 U/mL DNase I (2212, Takara, Dalian, China) at 37 °C for 10 min with intermittent agitation every 5 min ...
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bioRxiv - Plant Biology 2020Quote: A 20 μl reaction system with SYBR Green Supermix (Takara) was used for qRT-PCR analysis ...
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bioRxiv - Cancer Biology 2022Quote: ... 4.1 μL of RNase A (20 mg/mL; Clontech, 740505) was added ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were treated overnight with 20 µg Doxycycline (Takara Bio) per plate.
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bioRxiv - Genomics 2024Quote: ... 1) We added 20 µl Recombinant RNase Inhibitor (Takara, 2313A) into 4 ml EZ prep lysis buffer (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... 72°C for 15 s performed using a Takara Thermal Dice Real-time system III (Takara) or a QuantStudio 6 Flex Real-time PCR system (Applied Biosystems ...
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bioRxiv - Genetics 2022Quote: ... Each 30 µL PCR reaction volume contained 15 µL Premix Ex Taq enzyme (Takara Biomedical Technology), 0.2 µL BSA ...
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bioRxiv - Neuroscience 2024Quote: 14-15 DIV cultured cortical neurons were transfected with CalPhos mammalian transfection kit (Takara, cat # 631312). Crystals were washed and replaced with 2:1 conditioned media ...
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bioRxiv - Neuroscience 2024Quote: ... The non-solubilized fraction was separated by centrifugation at 47850 x g for 1 h at 4 °C and 4 mL of cobalt-charged TALON resin (Clontech, Takara) was added to the solubilized fraction for batch binding (3 h at 4 °C) ...
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bioRxiv - Plant Biology 2021Quote: ... whereas selective media additionally lacked His (−4) (Clontech).
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bioRxiv - Molecular Biology 2020Quote: ... 4 μL of 5x PrimeScript buffer (Takara, USA), 1 μL RNAse OUT (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 ml of Lenti-X concentrator (Takara Bio) was added to 12 ml of cell supernatant and the mixture incubated at 4°C with gentle agitation for 18 hours ...
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bioRxiv - Genomics 2022Quote: ... 3.5-4 million Lenti-X cells (Takara Bio) were seeded into 10 cm tissue culture dishes (Corning ...
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bioRxiv - Molecular Biology 2024Quote: ... and 4 U recombinant inhibitor (Cat. 2313B, TaKaRa) or SEQURNA thermostable RNase inhibitor (Cat ...
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bioRxiv - Bioengineering 2024Quote: ... and secretion signal 4 from pBIC4 (Takara, Japan) were incorporated upstream of the Lc and Hc genes ...
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bioRxiv - Cell Biology 2024Quote: ... with 10% FBS (Clontech) and 1% penicillin- streptomycin (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: Cells cultured in either 12- or 24-well plates were washed twice with cold PBS and harvested using a TaKaRa MiniBEST universal RNA extraction kit (Takara, Japan). RNA was purified using the same kit according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Cells cultured in either 12 or 24-well plates were washed twice with cold PBS and harvested using TaKaRa MiniBEST Universal RNA extraction kit (Takara, Japan). RNA was purified using the same kit according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... seedlings were subjected to 200 mM salt stress for 24 h and total RNA was isolated by RNAiso plus (Takara, China). Subsequently ...
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bioRxiv - Molecular Biology 2021Quote: ... In selected experiments cells would be then transfected with a siRNA-resistant construct and recovered for 24 hours prior to induction with 1 μM Shield1 (Takara Bio) and 1 μM 4-hydroxytamoxifen (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... NM_001123007) was amplified from 24 hpf embryo RNA using the PrimeScript II High Fidelity One Step RT-PCR kit (Takara, R026A) and the following primers ...
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bioRxiv - Neuroscience 2023Quote: ... The virus particles containing media were collected 24 h and 48 h after transfection and were concentrated using Lenti-X concentrator (Takara, 631232). The virus containing media was resuspended in Neurobasal media and tittered by puromycin selection using HEK 293T cell line ...
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bioRxiv - Immunology 2023Quote: ... Retroviral supernatants were then collected and spun at 2,000g for 2 h at 32 °C onto 24-well non-tissue-culture-treated plates coated overnight in Retronectin (Takara Bio) or spinfected for 90 minutes at 32°C in 1 µcg mL-1 polybrene ...
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bioRxiv - Cancer Biology 2023Quote: Reverse Transcriptase PCR and Real-Time PCR Cells cultured in either 12- or 24-well plates were washed twice with cold PBS and harvested using a TaKaRa MiniBEST universal RNA extraction kit (Takara, Japan). RNA was purified using the same kit according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... 152 ∗106 GPRTG cells were seeded into a 2-STACK culture chamber (Cornig) and transfected 24 h later with 238 µg transfer plasmid using the CalPhos Mammalian Transfection Kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... concentrated lentivirus (MOI: 25) was added to non-tissue culture treated 24-well plates that were coated with retronectin (Takara T100B) according to the manufacturer’s instructions and spun at 1,200 × g for 90 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... from second to third - 5’-ttgaattcgcgctttgtgagcattgc-3’ and 5’-ttgtcgacgttgtcgtccgtgtgcac-3’ and then subcloned into pGAD424 vector (Clontech) in frame with activation domain of GAL4 using restriction sites EcoR1 and Sall.
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bioRxiv - Biochemistry 2023Quote: ... ∼5 μg of bacmid were transfected using 5 μL of TransIT®-Insect transfection reagent (Takara Bio Inc.). 5 days after initial transfection ...
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bioRxiv - Microbiology 2023Quote: ... 5’RACE was performed with SMARTer RACE 5’/3’ Kit (Takara Bio USA, Inc. San Jose, CA USA) according to the manufacturer’s directions.
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bioRxiv - Biophysics 2024Quote: ... for 35 min at 4°C and the supernatant was incubated overnight on the rotisserie at 4°C with TALON Cobalt Resin (Takara Bio). The next day the resin was washed with 10 column volumes of Purification Buffer and 10 column volumes of Purification Buffer with 10 mM imidazole before elution using Purification Buffer with 300 mM imidazole ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was then diluted 1:4 to improve binding efficiency and incubated overnight on the rotisserie at 4°C with TALON Cobalt Resin (Takara Bio). The next day the resin was washed with 10 column volumes of Membrane Buffer 2 and 10 column volumes of Membrane Buffer 2 with 20 mM imidazole before elution with Membrane Buffer 2 with 300 mM imidazole ...
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bioRxiv - Microbiology 2024Quote: ... After co-cultivation, the organoids were collected and resuspended in 4% paraformaldehyde (PFA, Servicebio, China) at 4 [or RNAiso Plus (Takara, Japan) at −80□ for further analysis ...
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bioRxiv - Neuroscience 2024Quote: ... The non-solubilized fraction was separated by centrifugation at 47850 x g for 1 h at 4 °C and 4 mL of cobalt-charged TALON resin (Clontech, Takara) was added to the solubilized fraction for batch binding (3 h at 4 °C) ...
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Expansion of gamma-butyrolactone signaling molecule biosynthesis to phosphotriester natural productsbioRxiv - Biochemistry 2020Quote: ... 5’-ctcgagtgcggccgcaagcttgctcatgacgctgccacggtg-3’ using PrimeSTAR HS (Takara). The pET28a was digested at NdeI and HindIII sites ...