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Citations for Takara Bio :
151 - 200 of 631 citations for Alpha Cyclodextrin Solution 5% w v since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... the crude viral solution was concentrated using Lenti-x concentrator (Takara, cat.631232) to a final concentration of 108-109 IFU/ml using the Lenti-x qRT-PCR Titration Kit (Takara ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were pre-hybridised at 50 °C in ExpressHyb Hybridization Solution (Clontech, USA) for 1 h ...
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bioRxiv - Microbiology 2021Quote: ... Hybridisation was performed overnight at 50 °C in ExpressHyb Hybridisation Solution (Clontech, USA). After hybridisation ...
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bioRxiv - Bioengineering 2020Quote: ... and lentiviral vectors were concentrated 50-fold using Lenti-X concentrator solution (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... After cross-linking membranes were pre-hybridised in ExpressHyb Hybridization Solution (Clontech, USA) at 40 °C for 1 h ...
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bioRxiv - Molecular Biology 2023Quote: ... media was removed and concentrated using lenti-X concentrator solution (Takara Bio #631232). Concentrated viral particles were distributed into single-use aliquots and stored at −80°C until use ...
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bioRxiv - Biochemistry 2023Quote: ... followed by incubation with Takara POD conjugate solution and DAB substrate (Takara, Japan).
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bioRxiv - Developmental Biology 2024Quote: ... Membranes were UV cross-linked and pre-hybridized with ExpressHyb Hybridization Solution (Clontech) for 1 h at 50°C ...
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bioRxiv - Neuroscience 2022Quote: ... where the lysing solution is composed of single-cell lysis buffer (Takara #635013): 1x RNase Inhibitor Murine (NEB) ...
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bioRxiv - Plant Biology 2022Quote: ... purified with a high-salt solution for precipitation (Takara Bio Inc., Ohtsu, Japan) and reverse transcribed with ReverTra Ace (Toyobo) ...
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bioRxiv - Neuroscience 2024Quote: ... The standard curve was prepared using the positive control solution provided by Takara with serial dilution for absolute quantification ...
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bioRxiv - Molecular Biology 2024Quote: ... Crosslinked membranes were prehybridized for ∼1 hour at 55C in hybridization solution (Takara). DNA oligos (Table S1 ...
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bioRxiv - Molecular Biology 2024Quote: ... were hybridized with specific 32P-labeled probes (Table S3) in ExpressHyb solution (Clontech) for 2 hours at 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... Eluted solution was then loaded on TALON resin (Takara Bio Inc., Kusatsu, Japan) (bead volume ...
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bioRxiv - Molecular Biology 2020Quote: ... was amplified using specific primers (forward: 5’-AAGGAGATATACATATGCTCTCCGAAATGGTGGAAGAAG-3’; reverse:5’-GTGCGGCCGCAAGCTTTTATTTCTTTTTGTTGGTGGTCTG-3’) and cloned into pET28a using an InFusion HD Cloning Kit (Takara Bio USA) as per the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... To determine the 5’ and 3’ UTR of the sds3 gene 5’ and 3’ Rapid amplification of cDNA ends (RACE) was performed using the SMARTer® RACE 5’/3’ Kit (Takara Bio) on RNA isolated using Trizol (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... Primers used for amplifying the insert (5’-TACTTCCAATCCAATGTAGATATAAACAACAATAAGATTAGC-3’ and 5’- TTATCCACTTCCAATGAGATAACCTTGTACATCATCTGTATGC-3’) contained 15-bp overhang with homology to the plasmid for InFusion cloning (Takara Bio, 638947). The resulting plasmid ...
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bioRxiv - Microbiology 2021Quote: ... Primers used to amplify this region (5’-ATTGCGACACGTACTCTGCAGATCTCATACCATCATAGTTATAATATTAGC-3’ and 5’-AGAGGATCCCCATGGCTGCAGACACAGGTGTCGTCATTGTGA-3’) contained 15-bp overhang with homology to the plasmid for InFusion (Takara Bio, 638947) cloning and retained the Pst1 site ...
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bioRxiv - Microbiology 2023Quote: The 5’ and 3’ termini of the sRNA OueS were determined by the RACE assay (SMARTer RACE 5’/3’ kit, Takara Bio USA), adapted for non-poly-A-tailed RNA ...
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bioRxiv - Plant Biology 2022Quote: ... The qRT-PCR was performed with the reversed cDNAs as substrates and the MeSWEET10a specific primers (F: 5’-TCCTCACCTTGACTGCGCTG-3’; R: 5’-AGCACCATCTGGACAATCCCA-3’) by using the SYBR Premix Taq Kit (TaKaRa, Dalian, China) in the ABI7500 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2024Quote: The 5’ and 3’ termini of the sRNA OueS were determined by the RACE assay (SMARTer RACE 5’/3’ kit, Takara Bio USA), adapted for non-poly-A-tailed RNA ...
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bioRxiv - Microbiology 2022Quote: ... The 5 mL HisTALON cartridge (Clontech Laboratories, Cat# 635683) column was equilibrated with ten column volumes of Equilibration Buffer (HisTALON Buffer Set ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... 5 μL of Premix Ex Taq (Takara, Dalian, China), and 1.5 μL of a mixture of probe (5’-TGCAC GTTGT GACAG TCGT-3’ ...
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bioRxiv - Genomics 2021Quote: ... 5 μL of 2.5 mM dNTPs (Takara Bio Inc.), 1 μL of 20 μM TruSeqUniv (Supplementary Table S7) ...
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bioRxiv - Genomics 2021Quote: ... 5 μL of 10× ExTaq buffer (Takara Bio Inc.), 5 μL of 2.5 mM dNTPs (Takara Bio Inc.) ...
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bioRxiv - Genetics 2020Quote: ... 5′-phosphorylated by T4 polynucleotide kinase (TaKaRa, Shiga, Japan), self-ligated by T4 ligase (TaKaRa ...
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bioRxiv - Microbiology 2021Quote: ... The 5 mL HisTALON cartridge (Clontech Laboratories, Cat # 635683) column was equilibrated with 10 column volumes of Equilibration Buffer (HisTALON™ Buffer Set ...
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bioRxiv - Bioengineering 2023Quote: ... and 5 U of Taq DNA polymerase (TaKaRa Bio) was subjected to PCR using a thermal cycler (Applied Biosystems 7300 real-time PCR system ...
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bioRxiv - Plant Biology 2022Quote: ... 5 units of recombinant DNase I (TaKaRa, Kyoto, Japan), 200 units of RevertAid™ reverse transcriptase (Thermo Scientific ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 5 µL of 2.5 mM dNTPs (Takara #4025), with the following thermal cycle condition ...
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bioRxiv - Molecular Biology 2024Quote: ... and then 5 μL 10× Klenow fragment buffer (Takara), 5 μL dNTP mix (0.2 mM dATP ...
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bioRxiv - Plant Biology 2023Quote: ... The gRNA was then ligated into BsaI linearized pKSE401 using DNA ligation Kit v.2.0 (TaKaRa, Kusatsu, Shiga, Japan).
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bioRxiv - Plant Biology 2021Quote: Transcription start sites were characterised by cloning and sequencing 5’ RACE products using the SMARTer RACE 5’/3’ kit (Takara Bio USA, Inc). In summary ...
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bioRxiv - Plant Biology 2023Quote: The cDNA prepared from 10-day-old OsbZIP48OE seedlings was used to perform 5’ and 3’ RACE using SMARTer® RACE 5’/3’ Kit (Clontech, TaKaRa Bio, USA) as per manufacturer’s instructions followed by Sanger based sequencing of the RACE products ...
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bioRxiv - Microbiology 2023Quote: ... which was amplified by PCR from human cDNA using primers forward (5’-CAGTGTGGTGGAATTCACCATGTCAAGCTCTTCCTGGCT-3’) and reverse (5’-CACAAGATTTGGGCTCGGAAACAGGGGGCTGGTTAG-3’) and cloned into plasmid pVAX-IGHG1ΔCH1 by In-Fusion cloning (Takara Bio, San Jose, CA). This plasmid contains an Fc domain comprising Kabat numbers 226-478 of human IgG1 ...
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bioRxiv - Molecular Biology 2022Quote: ... the 5′ and 3′’ untranslated regions (UTRs) of the virus were determined using a SMARTer®RACE 5′/3′ Kit (Takara Bio, USA). Viral RNA isolated in August ...
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bioRxiv - Molecular Biology 2024Quote: ... The 21 bp barcodes were amplified with 5’-GGGATCACTCTCGGCATGG-3’ forward and 5’-CTGATCAGCGAGCTCTAGGAA-3’ reverse primers and PrimeSTAR GXL DNA polymerase (Takara Bio, Shiga, Japan). They were then sequenced with NextSeq 500 1×150 (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... was used to determine 5’ and 3’ terminal nucleotide sequences of PcAEV4 and PcAEV5 with the SMARTer® RACE 5’/3’ Kit (Takara Bio USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and hybridisation was performed overnight at 40 °C in ExpressHyb Hybridisation Solution (Clontech, USA). Membranes were then rinsed and washed at 40 °C 4 x 15 min with a wash buffer containing 1% sodium dodecyl sulphate [SDS] and 1x saline-sodium citrate (SSC) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were harvested and AAV2 was isolated using AAVpro Extraction Solution (Takara, Catalog #6235). Two million NC797 cells were infected with 30ul of each adeno-associated virus (AAV2) ...
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bioRxiv - Neuroscience 2023Quote: ... and then the virus solution was concentrated using the Lenti-X Concentrator (631231, Clontech) and suspended in PBS (in vivo conditions).
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bioRxiv - Microbiology 2023Quote: ... The solution was mixed with DNA Ligation Kit
(6023, Takara Bio Inc.) and used for transformation with NEB 5-alpha F′Iq Competent E ... -
bioRxiv - Genetics 2023Quote: ... The viral solution was further concentrated using the Lenti-X™Concentrator (Takara: 631231) according to the manufacturer’s instructions and stored at -80°C for long-term use ...
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bioRxiv - Genomics 2023Quote: ... cells were harvested and AAV2 was isolated using AAVpro Extraction Solution (Takara, Catalog #6235). Two million NC797 cells were infected with 30ul of each adeno-associated virus (AAV2) ...
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bioRxiv - Molecular Biology 2024Quote: ... Hybridization was performed according to the manufacturer’s manual using ExpressHyb Hybridization Solution (Takara Bio) and the radioactive signal was detected using a Phosphorimager system ...
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bioRxiv - Plant Biology 2023Quote: The cDNA prepared from 10-day-old OsbZIP48OE seedlings was used to perform 5’ and 3’ RACE using SMARTer® RACE 5’/3’ Kit (Clontech, TaKaRa Bio, USA) as per manufacturer’s instructions followed by Sanger based sequencing of the RACE products ...
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bioRxiv - Neuroscience 2023Quote: ... 5′-GCATGTCACGATGTTACAA-3′ and 5′-GGATGAAATGAAGGTGCTA-3′ as previously described50 and were inserted using the Infusion HD Cloning kit (Takara Bio USA Inc, NC1470242).
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bioRxiv - Cancer Biology 2023Quote: Coding regions of the heavy-chain variable domains were amplified with two PAGE-purified primers (CALL001: 5’-GTCCTGGCTGCTCTTCTACAAGG-3’ and CALL002: 5’-GGTACGTGCTGTTGAACTGTTCC-3’) using LA Taq polymerase (TAKARA Bio Inc., Shiga, Japan). The amplified fragments were separated on a 1.5% low-melting-temperature agarose gel (Lonza Group AG ...
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bioRxiv - Neuroscience 2024Quote: ... Genomic recombination assay was performed using PCR primers that flank exons 4-6 of mouse Galnt2 (F: 5’-GTACGTGAGACAGGCCTAAGG-3’ R: 5’-CAAGCTTCATTTAGGACCAAGC-3’) and EmeraldAmp PCR Master Mix (Takara Bio, San Jose, CA). PCR cycling parameters were as follows ...
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bioRxiv - Immunology 2020Quote: ... and mCherry was amplified from pTRE-Dual2 (Clontech # PT5038-5). pAF137 was constructed by amplifying the devil 41BB extracellular domain with primers pAF137-1.FOR and pAF137-1.REV and amplifying mCherry with pAF137-2a.FOR and pAF137-2.REV (Table S3-4) ...