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Citations for Takara Bio :
1651 - 1700 of 1758 citations for Zika Virus Vero Cell Lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA was isolated from vehicle and Lantana camara leaf ethanolic extract-treated MDA-MB-231 cells using RNAiso Plus (Takara, Japan) as per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was extracted from cells and reverse-transcribed to cDNA using the PrimeScript 1st Strand cDNA Synthesis Kit (TaKaRa Bio). cDNA was used as a template for real-time qPCR analysis on CFX96 Touch (Bio-Rad ...
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bioRxiv - Developmental Biology 2023Quote: ... Quantitative RT-PCR for pluripotency and trilineage spontaneous differentiation was performed according to the instruction manual of the human ES cell Primer Array (Takara Clontech).
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bioRxiv - Developmental Biology 2023Quote: ... Only 0.5 µL of media containing cumulus cells from each mouse was added to 10 µL lysis buffer (Takara Bio Inc.). The oocytes were then treated with 1 mg/mL collagenase to remove the ZP ...
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bioRxiv - Developmental Biology 2024Quote: ... for 20 minutes on ice and then dispensed into the nano-well plates of the ICELL8® cx Single-Cell System (Takara). Wells containing single viable cells were automatically selected using the ICELL8 cx CellSelect v2.5 Software (Takara ...
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bioRxiv - Cancer Biology 2023Quote: ... and clonally isolated p53 KO cell lines were electroporated with 3 µg p53 firefly luciferase reporter plasmid pp53-TA-Luc (Clontech/Takara) and 0.3 ug renilla luciferase control plasmid pRL-CMV (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... suspensions of the transformants (1.0×106 cells) were spotted onto SD plate media with dropout supplements,-Leu/-Trp or-Ade/-His/-Leu/-Trp (cat #630428) (Takara Bio), and incubated at 30°C for 3 days.
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bioRxiv - Cell Biology 2024Quote: Total RNAs from HeLa cells (about 5 x 107 cells/10 cm dish) transfected with scrambled siRNA (si-Ctrl) or si-CDC5L were extracted using RNAiso Plus (Takara, 9109) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2024Quote: ... the U2OS 2-6-3 cells expressing degron-tagged LacI fusion proteins were incubated with 300 nM Shield1 ligand (Takara Bio) and 1 µg/mL doxycycline for 24 hours ...
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bioRxiv - Microbiology 2024Quote: ... reactions were performed to insert these linear fragments into the pMV306 previously digested with EcoRV and transformed into Escherichia coli (E. coli) Stellar TM competent cells (Takara Bio), purified (NucleoSpin Plasmid ...
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bioRxiv - Biochemistry 2024Quote: ... Recombinant His-tagged protein was purified from cell-free extracts using immobilized metal affinity chromatography (IMAC using Talon resin; Takara Bio). The buffer used during the purification process was 20 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2023Quote: ... In short: 3.5 cm non-coated cell culture dishes (Falcon, cat 351008) were coated using 12 µg Retronectin (Takara, cat T100B). Coated dishes then were pre-incubated with 1 ml viral supernatant for 4-5 hours at 37oC ...
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bioRxiv - Bioengineering 2024Quote: AAV-vector plasmid and AAV-crTSG were used for AAV9 production and purification using Lenti-X 293 T cells (Takara Bio). Each set of Lenti-X 293 T cells (6 150mm-plates ...
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bioRxiv - Cancer Biology 2024Quote: ... 4T1 cells were transduced with a luciferase cDNA cloned into the pQCXIN retroviral expression vector (Takara Bio USA; San Jose, CA), selected with 0.5 mg/ml G418 ...
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bioRxiv - Microbiology 2020Quote: ... The PCR product was then cloned into the BG1861 vector by ligation-independent cloning to introduce a N-terminal 6xHis tag and transformed into Stellar™ chemically competent cells (Clontech Laboratories) for plasmid propagation (84) ...
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bioRxiv - Immunology 2021Quote: ... cDNA was synthesized and amplified directly from intact cells using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Bio USA) according to the manufacturer protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... LNCaP-C4-2 cells were transduced to express ZsGreen or Firefly luciferase-mCherry by the Wake Forest Baptist Comprehensive Cancer Center (WFBCCC) Cell Engineering Shared Resource using a lentivirus (Clontech or Genecopoeia). LNCaP-C4-2 and PC3-mm were sorted into CD117+ and negative populations using the Miltenyi MACS sorting beads or the ThermoFisher MagniSort CD117 (c-kit ...
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bioRxiv - Neuroscience 2021Quote: ... 2 days post-treatment the vehicle control flasks had DNA from 1×108 cells per replicate isolated with Nucleobond CB 500 kit (Takara Bio 740509) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... were co-transfected into HEK293T cells at a 1:1:1:1.6:4.6 ratio using CalPhos mammalian transfection kit (TaKaRa Clontech, Mountain View, CA #631312) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: SH-SY5Y cells with inducible expression of GDE2 constructs were generated using the Retro-X™ Tet-On® Advanced Inducible Expression System (ClonTech), as described (Matas-Rico et al. ...
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bioRxiv - Microbiology 2021Quote: ... HEK293T cells grown in 10 ml of medium (3.0 x 105 cells/ml) were transfected with 10 µg of pCAGGS-NP (1-450) using TransIT-293 Reagent (Takara, Shiga, Japan). Three days post-transfection ...
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bioRxiv - Genomics 2020Quote: ... cells were loaded onto a source plate and dispensed in to a SMARTer ICELL8 350 v chip (Takara Bio USA, Cat. # 640019) at 35 nanoliter per well ...
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bioRxiv - Neuroscience 2021Quote: ... The supernatant was removed and the cell pellet re-suspended with a 20 µL mix containing: 1X of the 5X PrimeSTAR GXL Buffer (Clontech, Takara Bio Europe), proteinase K (0.167 mg/mL ...
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bioRxiv - Cell Biology 2021Quote: 5’ and 3’ RACE reactions were performed to isolate full-length lncEry from the total RNA of MEP cells using the 5’- and 3’-Full RACE Kits (TaKaRa, Dalian, China) in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... were gently dissociated with 0.05% Trypsin and plated onto 0.1% gelatin coated cell culture dish at a density of 1×104cells / cm2 with RHB-A medium (Clontech TaKaba Cellartis, Y40001). Medium was changed every 2 days and cultured for 4 days ...
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bioRxiv - Cancer Biology 2022Quote: RFP-NLS expressing iHFK cells were generated by transfecting with the RFP-NLS expression plasmids using Xfect transfection reagent (Takara Bio; 631317). Cells containing RFP-NLS plasmids were selected using 1 μg/ml puromycin ...
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bioRxiv - Microbiology 2022Quote: ... HEK293 cells were cotransfected with the S expression plasmids (400 ng) and pDSP8-11 (400 ng) using TransIT-LT1 (Takara, Cat# MIR2300). To prepare target cells ...
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bioRxiv - Bioengineering 2022Quote: 500 ng of total RNA extracted from cell culture were subjected to reverse transcription, using ISOGEN II (NIPPON GENE, Tokyo, Japan) and PrimeScript RT reagent Kit (TaKaRa, Kusatsu, Japan). The resulting cDNA was subjected to real-time PCR analysis using glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Microbiology 2021Quote: ... this results in a similar τ constant to standard settings on other instruments of 25 μF capacitance and 200 Ω resistance). Cells were immediately rescued with 1 ml of room temperature (ca. 23°C) SOC media (Takara Bio) and recovered by incubating with shaking for 1 hour at 37°C.
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bioRxiv - Microbiology 2020Quote: ... Cell supernatants were harvest 24 hours post-infection and RNA was extracted by a viral RNA extraction kit (Takara, Cat no. 9766). The number of viral copies was detected by real-time quantitative reverse transcription PCR (qRT-PCR ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were later processed for single cell RNA-seq using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Cat# 634894) as previously described41,45.
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bioRxiv - Cell Biology 2022Quote: Total RNA was extracted from cells treated with either buffer or Mindin for 16-hours using TRIzol Reagent (TaKaRa, Thermo Fisher Scientific) and cDNA was synthesised using Superscript III (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2019Quote: Single cells were stained with Hoechst 33342 and Propidium Iodide according to the manufacturer’s protocol (Clontech iCell8 D07-000040-004 rev 4). Before cell loading ...
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bioRxiv - Genomics 2019Quote: ... Full-length cDNA fragments were transcribed and amplified from poly-A RNA in each single cell using the SMARTer Ultra Low RNA kit (Takara Bio, 634832). Tagmentation of cDNA was performed and sequencing libraries were prepared using the Nextera XT DNA sample preparation kit (Illumina ...
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bioRxiv - Microbiology 2019Quote: ... Low cell RNA-seq libraries (unstranded) wer generated withSMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara/ Clonetech; Cat#634890). All sequencing libraries were sequenced on a HiSeq 2500 system (Illumina ...
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bioRxiv - Molecular Biology 2020Quote: Libraries from RPFs isolated from HEK293T cells were prepared using the SMARTer® smRNA-Seq Kit for Illumina (Takara, Cat. No. 635029) and sequenced with 50 cycles single-read on an Illumina NovaSeq 6000 sequencer ...
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bioRxiv - Immunology 2021Quote: ... Full-length mIDO1 gene was PCR amplified from cDNA of IFN-γ-treated ID8 cell line (26) and inserted into the first cloning site in a retrovirus backbone vector (pQCXIX, Clontech-TaKaRa). A fusion gene of codon-optimized Luciferase (Luc2 ...
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bioRxiv - Plant Biology 2020Quote: ... while specific AD and BD recombinant plasmid pairs were used to co-transform yeast strain AH109 cells according to the Yeast Protocols Handbook (Takara, Dalian, China).
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bioRxiv - Cell Biology 2020Quote: ... at the concentration of 25 cells/μl in 0.5× PBS and 1× Second Diluent (640202, Takara Bio USA, Mountain View, CA, USA) into the ICELL8® 350v Chip (640019 ...
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bioRxiv - Immunology 2021Quote: ... TM cells were directly sorted into 1X lysis buffer and cDNA was synthesized and amplified directly from intact cells using SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Bio USA) according to the manufacturer protocol ...
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bioRxiv - Plant Biology 2022Quote: ... The DNA encoding the Piks-HMAs in pGBKT7 was co-transformed with either the individual AvrPik variants in pGADT7 into chemically competent Saccharomyces cerevisiae Y2HGold cells (Takara Bio USA). The single colonies grown on selection plates were inoculated in SD-Leu-Trp plate and grown at 30 °C for overnight ...
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bioRxiv - Plant Biology 2023Quote: ... an aliquot (100 µL) of transformed cells was then plated onto SD media containing the appropriate dropout supplement (Takara Bio USA, Inc.), and grown at 30°C for up to 7 days.
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bioRxiv - Immunology 2022Quote: Total RNA was isolated from RAW264.7 cells and ground cartilage from human tibial plateaus using TRIzol reagent (Takara Bio Inc., Shiga, Japan). For mRNA quantification ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Inducible expression of a Nef-eGFP fusion protein was established in CEM-T4 cells using the Lenti-XTM Tet-On System (Clontech now Takara Bio USA) which places expression of Nef-eGFP under the control of a doxycycline-inducible promoter ...
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bioRxiv - Cell Biology 2023Quote: The cDNA of sorted tdTomato positive hair cells was synthesized using PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time, RR047A, Takara Bio). Synthesized cDNA was subsequently mixed with qPCR primers and TB Green Premix Ex Taq (Tli RNase H Plus ...
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bioRxiv - Microbiology 2023Quote: ... ISG20 expression was routinely induced upon incubation of cells for 18 hours with 1.5 μg/mL of doxycycline (Takara Bio, cat. 631311).
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bioRxiv - Microbiology 2023Quote: The cDNA encoding human ZCCHC3 (NM_033089.7) was amplified from cDNA pool of HeLa cells by PCR and cloned into pEGFP-C3 (Takara Bio Inc., Shiga, Japan), pCMV-HA-N2 (Takara Bio Inc.,) ...
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bioRxiv - Immunology 2023Quote: ... Plasmids were packed into lentiviral particles using the Lenti-X cell line and Lenti-X Packaging Single Shots (VSV-G) (Takara, Cat.No. 631276) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Division of AIDS, NIAID, NIH (contributed by Dr. John C. Kappes, Dr. Xiaoyun Wu and Tranzyme Inc.) Lenti-X 293T cells (Takara Bio Inc.), TZM-bl cells ...
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bioRxiv - Cancer Biology 2023Quote: BON1/HA-Bcl-xL/TR-shCtBP2 and BON1/HA-Bcl-xL/TR-shRLuc #713 cells were treated with 1 µg/ml (before sorting) or 0.5 µg/ml (after sorting) doxycycline (Clontech Laboratories, Inc., 631311) for 96 hours ...