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Citations for Takara Bio :
101 - 150 of 747 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... anti-His (1:5000, #631212, Takara), anti-Erk1/2 (1:2000 ...
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bioRxiv - Immunology 2021Quote: ... supernatants were harvested by centrifugation and further purified using immobilized metal affinity chromatography (IMAC) with cobalt-TALON resin (Takara) followed by Superdex 200 Increase 10/300 GL size exclusion column (GE Healthcare).
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bioRxiv - Immunology 2021Quote: ... Protein was purified in a PBS buffer (pH 7.4) from filtered supernatants by using either StrepTactin resin (IBA) or Cobalt-TALON resin (Takara). Affinity tags were cleaved off from eluted protein samples by HRV 3C protease ...
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bioRxiv - Immunology 2021Quote: ... Protein was purified in a PBS buffer (pH 7.4) from filtered supernatants by using either StrepTactin resin (IBA) or Cobalt-TALON resin (Takara). Affinity tags were cleaved off from eluted protein samples by HRV 3C protease ...
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bioRxiv - Immunology 2021Quote: ... His-tagged NTD domain constructs were purified from clarified supernatants using 2 ml of cobalt resin (Takara Bio TALON), washing with 50 column volumes of 20 mM HEPES-HCl pH 8.0 and 150 mM NaCl and eluted with 600 mM imidazole ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting cell lysate was used for affinity purification and flowed over a TALON cobalt affinity resin (Takara Bio). The cobalt resin was washed with ~20 column volumes (CV ...
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bioRxiv - Microbiology 2021Quote: ... H6-Gfp-FtsZ and FtsZ were mixed in a 1:1 ratio for a final concentration of 8 µM in assembly buffer with GMPCPP (0.2 mM) and resuspended Talon Cobalt beads (Takara) and added ...
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bioRxiv - Immunology 2022Quote: ... Protein was purified in a PBS buffer (pH 7.4) from filtered supernatants by using either StrepTactin resin (IBA) or Cobalt-TALON resin (Takara). Affinity tags were cleaved off from eluted protein samples by HRV 3C protease ...
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bioRxiv - Developmental Biology 2022Quote: ... Primary antibodies were anti-PLP (N-terminus; 1:5000, Rogers, 2008) anti-GFP (JL8; 1:2000 – 5000; Clontech); anti-alpha-Tubulin (DM1A ...
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bioRxiv - Immunology 2021Quote: ... supernatants were harvested by centrifugation and further purified using immobilized metal affinity chromatography (IMAC) with cobalt-TALON® resin (Takara) followed by Superdex 200 Increase 10/300 GL size exclusion column (GE Healthcare).
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bioRxiv - Molecular Biology 2021Quote: ... was then added to the supernatant to a final concentration of 5 mM and the solution was subjected to immobilized metal affinity chromatography (IMAC) by incubation with TALON cobalt resin (Takara) for 1 hr at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant protein were purified to >90% electrophoretic purity by immobilized metal ion affinity chromatography using a cobalt-based matrix (Talon, Clontech) and eluted with imidazole as described previously24 ...
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bioRxiv - Biochemistry 2021Quote: ... Purification procedure was similar to that for HEK 293 cells except for the usage of cobalt-based affinity resin (Talon, Clontech) to capture His-tagged proteins.
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bioRxiv - Biophysics 2023Quote: ... Proteins were purified via immobilized metal affinity chromatography (IMAC) with TALON metal affinity cobalt resin and its associated buffer set (Takara), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were lysed by sonication then purified using immobilized metal affinity chromatography (IMAC) resin charged with cobalt (TALON resin; Takara). Proteins were eluted with 200 mM Imidazole in buffer containing 20 mM Tris pH 7.5 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The recombinant proteins were purified using a TALON Metal (Cobalt) Affinity Resin column (Clontech Laboratories, Inc., Palo Alto, CA, USA) and eluted with a linear gradient of imidazole (0–1,000 mM ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV2 Mpro and C300S Mpro were purified first by affinity chromatography using TALON™ cobalt-based affinity Resin (Takara Bio). The His6-tag was cleaved off by PreScission protease and the resulting authentic 306 amino acid Mpro (see Figure S1A in supplemental material ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was then diluted 1:5 to improve binding efficiency and incubated overnight on the rotisserie at 4°C with TALON Cobalt Resin (Takara Bio). The next day the resin was washed with 10 column volumes of Membrane Buffer 1 ...
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bioRxiv - Biophysics 2024Quote: ... for 35 min at 4°C and the supernatant was incubated overnight on the rotisserie at 4°C with TALON Cobalt Resin (Takara Bio). The next day the resin was washed with 10 column volumes of Purification Buffer and 10 column volumes of Purification Buffer with 10 mM imidazole before elution using Purification Buffer with 300 mM imidazole ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was then diluted 1:4 to improve binding efficiency and incubated overnight on the rotisserie at 4°C with TALON Cobalt Resin (Takara Bio). The next day the resin was washed with 10 column volumes of Membrane Buffer 2 and 10 column volumes of Membrane Buffer 2 with 20 mM imidazole before elution with Membrane Buffer 2 with 300 mM imidazole ...
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bioRxiv - Biochemistry 2023Quote: ... Affinity purification of Ypq1-3C-GFP-8xHis was done by incubating the supernatant with 3 mL TALON cobalt resin (635502; TaKaRa Bio) pre-equilibrated with Column buffer (20 mM HEPES-KOH (pH 7.2) ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit anti-dsRed (Clontech/Takara 632496, 1:5000), rabbit anti-MyosinVIIA (Proteus Biosciences 25-6970 ...
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bioRxiv - Developmental Biology 2023Quote: ... rabbit anti-dsRed (Clontech/Takara 632496, 1:5000), rabbit anti-MyosinVIIA (Proteus Biosciences 25-6970 ...
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bioRxiv - Microbiology 2020Quote: ... coli BL21 (Takara Bio Co Ltd, Japan) beared the recombinant vectors were inoculated in LB medium containing 100 μg/ml carbenicillin ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The decanted soluble extract was adjusted to a volume of 90 mL with lysis buffer and incubated with 10 mL TALON cobalt resin (Takara, Shiga, Japan) for 1 h at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... Cell debris was pelleted down and the supernatant was run on a 2 mL column volume (CV) TALON cobalt affinity resin (Takara Bio #635504) equilibrated in CoWB/TCEP (100 mM NaCl (Sigma-Aldrich 746398) ...
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bioRxiv - Microbiology 2024Quote: ... the supernatant obtained by centrifugation at 12,000 × g for 20 min at 4°C was applied to the column purification using immobilized metal affinity chromatography (IMAC) with a cobalt ion carrier (TALON metal affinity resin, Takara Bio Inc.). Antiserum was produced in a rabbit by immunization with the FoIV1-rORF4 (Eurofins Genomics).
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bioRxiv - Plant Biology 2024Quote: ... The dilutions were plated on selective -LT medium as a growth control and on -LTH medium (7.6 g/L Yeast Nitrogen Base [YNB], 20 g/L glucose, 0.62 g/L DO supplement -leu -trp -his [Clontech], 15 g/L agar) for the protein interaction assay ...
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bioRxiv - Cell Biology 2022Quote: Primary antibodies against GFP (Clontech, JL-8; 1:5000), G-6-PDH (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: pVAX1-SARS-CoV2-co was designed by Takara, which encoded a highly optimized DNA sequence encoding the SARS-CoV-2 Spike glycoprotein ...
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bioRxiv - Molecular Biology 2020Quote: ... or PrimeSTAR GXL DNA polymerase kit (Takara Co.). The amplicons were subcloned into the TOPO™ vector (Invitrogen ...
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bioRxiv - Developmental Biology 2022Quote: ... were co-transfected into 293T cells (Clontech, 632180) with PEI reagent (Polysciences ...
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bioRxiv - Pathology 2024Quote: ... SYBR Green PCR reagent (Takara Co. Ltd., Japan) was chosen for real-time fluorescence quantification ...
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bioRxiv - Genetics 2021Quote: ... and a 1:5000 dilution of anti-GFP (Takara, 632381). Blots were washed (3 × 10 mins ...
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-GFP (Clontech, JL-8, 1:5000 for western), rabbit anti-GFP (Chromotek ...
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bioRxiv - Neuroscience 2022Quote: ... a mouse anti-mCherry primary antibody (Takara Bio, 1:5000) was used instead of the substance P primary antibody to visualize hM3Dq-mCherry or mCherry-expressing cells.
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-GFP (Clontech, JL-8, 1:5000 for western), mouse anti-Dhc (Developmental Studies Hybridoma Bank ...
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bioRxiv - Microbiology 2024Quote: ... which detects GFP in our study (Clontech, 63281; 1:5000), goat anti-rabbit IgG secondary antibody (Fisher ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were co-transfected with pEGFP-F (Clontech), pDsRed monomer-F (Clontech) ...
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bioRxiv - Biochemistry 2020Quote: ... were co-transformed with pGro7 plasmid (Takara Bio Inc.) into E ...
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bioRxiv - Developmental Biology 2023Quote: ... These plasmids were co-transfected with pEGFP-N1 (Clontech) into O9-1 using Lipofectamine 2000 (Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: HEK293EB cells were co- transfected with pAAV-ZsGreen1 (TaKaRa) or pAAV-UGI ...
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Pyruvate kinase M2 regulates Japanese encephalitis virus replication by interacting with NS1 proteinbioRxiv - Microbiology 2024Quote: Capturem Co-IP kit was procured from TaKaRa (Japan). The Neuro-2a cells were transfected with pEGFP-PKM2 and cells were infected with JEV 24 hours post-transfection ...
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bioRxiv - Neuroscience 2023Quote: ... 2 µg/ml doxycycline (Takara) was added on day 0 to induce TetO gene expression ...
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bioRxiv - Neuroscience 2024Quote: ... or 10 µg DsRedExpress (Clontech) together with 1-2 µg of GFP-tagged PSD95α41 or PSD93α42 ...
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bioRxiv - Neuroscience 2024Quote: ... Doxycycline (2 µg/ml, Clontech) was added on day 0 to induce TetO gene expression and retained in the medium until the end of the experiment ...
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bioRxiv - Molecular Biology 2024Quote: ... and pVSV-G (Clontech) constructs in 6:3:1 weight ratios by X-Treme Gene HP Transfection Reagent (Roche ...
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bioRxiv - Plant Biology 2021Quote: ... membranes were incubated in anti-GFP (Takara Bio, 1:5000 dilution) antibodies followed by a 2 h incubation in secondary goat anti-mouse-HRP (1:10,000 dilution ...
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bioRxiv - Plant Biology 2021Quote: ... immunoblot with an anti-GFP antibody (1:5000 dilution, Takara Bio) followed by and detection were performed as described (Basu ...
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bioRxiv - Genetics 2021Quote: ... probed with anti-GFP (Takara Bio Clontech, #632380, mouse, 1:5000) and anti-Tubulin (Sigma ...