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Citations for Takara Bio :
1401 - 1450 of 2365 citations for 6 Chloro 4 hydroxy 2 methyl 2H thieno 2 3 e 1 2 thiazine 3 carboxylic acid methyl ester 1 1 dioxide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: The high-quality human total brain RNA that was used for 3’ RACE was purchased from Clontech (Mountain View, CA). SCA12 KI-10 and KI-80 mouse models were generated using the CRISPR/Cas9 approach by replacing the mouse PPP2R2B exon 2 with the human PPP2R2B exon 7 containing either 10 or 80 CAG triplets (Li and Margolis ...
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bioRxiv - Microbiology 2023Quote: ... by PCR using the primers shown in S-Table 3 into the EcoRI and BamHI sites of pRetroX-TRE3G (TaKaRa). pBS-UGI-flag was constructed by amplifying the UGI and flag sequence from UGI- pFERp44 by PCR and cloning it into the NotI and EcoRI sites of pBluescript II KS(+ ...
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bioRxiv - Microbiology 2023Quote: ... pAAV-UGI was constructed by cloning the entire coding sequence of UGI amplified from pBS-UGI-flag by PCR using the primers shown in S-Table 3 into the EcoRI and BamHI sites of pAAV-ZsGreen1 (TaKaRa).
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bioRxiv - Molecular Biology 2023Quote: ... linearized by PCR and using the divergent primers reported in Supplementary Table 3 and CloneAmp™ HiFi PCR Premix (Takara) polymerase ...
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bioRxiv - Microbiology 2023Quote: ... reverse transcription of the RNA using a primer designed to the constant region of the barcoded adaptor with addition of an adapter to the 3’ end of the cDNA by template switching using SMARTScribe (Clontech) as described (23) ...
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bioRxiv - Immunology 2022Quote: ... cDNA was generated from 10 μl RNA according to the SMARTer RACE 5’/3’ manual using SMARTScribe Reverse Transcriptase (Takara) and a self-designed template-switch oligo (AGGGCAGTCAGTCGCAGNNNNWSNNNNWSNNNNWSGCrGrGrG) ...
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bioRxiv - Molecular Biology 2023Quote: ... synthesized 5’ and 3’ fragments of the target RNA were 32P-radiolabeled at the 5’ end using T4 polynucleotide kinase (Takara). After gel purification ...
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bioRxiv - Microbiology 2023Quote: ... The expression profile of target gene was evaluated using specific primers (Table-3) by using SYBR green RT-PCR master mix (Takara) in BioRad Real time PCR instrument ...
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bioRxiv - Microbiology 2024Quote: ... reverse transcription of the RNA using a primer designed to the constant region of the barcoded adaptor with addition of an adapter to the 3’ end of the cDNA by template switching using SMARTScribe (Clontech) as described;86 (ii ...
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bioRxiv - Cancer Biology 2023Quote: ... and clonally isolated p53 KO cell lines were electroporated with 3 µg p53 firefly luciferase reporter plasmid pp53-TA-Luc (Clontech/Takara) and 0.3 ug renilla luciferase control plasmid pRL-CMV (Promega ...
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bioRxiv - Molecular Biology 2024Quote: ... The expression vector was produced by inserting the cDNA encoding the Rhino fragment into pGEX-5X-3 (Cytiva) by Infusion (Takara). The primers used are listed in Supplementary Table 1.
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bioRxiv - Microbiology 2024Quote: ... and HHT1_U and HHT1_L for HHT1 (Table 3) using SYBR® Premix Ex TaqTM (Perfect Real Time) (Takara Bio Inc.) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... All 3 fragments were then ligated into pUC18T-mini-Tn7T digested with EcoRI and XmaI using the infusion kit (Takara). The deletion of bspD was complemented by cloning of a 2160 bp long sequence encoding bspD and its upstream region including eipA and its 5’ non-coding region harboring the CtrA-controlled promoter (22 ...
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bioRxiv - Cancer Biology 2022Quote: ... Rabbit polyclonal antibodies used were: HECD1 (anti-E-cadherin; Takara), anti-Melan-A (ab15468 ...
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bioRxiv - Developmental Biology 2024Quote: ... Primary antibodies used were CDH1 (E-CADHERIN) (M106, Takara Biosciences) for glandular and luminal epithelial staining ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 mM DTT and 400 units/ml of Recombinant RNase Inhibitor (TaKaRa)) with cOmplete (Roche ...
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bioRxiv - Neuroscience 2021Quote: ... the enhanced yellow fluorescent protein (YFP) from pEYFP-N1 (#6006-1, Clontech), the woodchuck hepatitis virus posttranscriptional regulatory element (WPRE ...
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bioRxiv - Plant Biology 2019Quote: ... Then 1 µg of RNA sample was used for reverse transcription (Takara). The resultant cDNA of corresponding genes and ACTIN7 was analysed using the SYBR Premix Ex Taq (Takara ...
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bioRxiv - Biochemistry 2019Quote: ... Dimerization of FKBP-FRB was induced with 1 µM rapalog (Takara Bio). Images were analyzed in ImageJ ...
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bioRxiv - Cancer Biology 2019Quote: ... control cells were transfected with 500 ng pEGFP-N3 (Clontech, # 6080-1). Transfection was performed using FuGENE HD transfection reagent (500 ng DNA ...
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bioRxiv - Developmental Biology 2019Quote: ... to 1 cell/50 nl for dispensing on the ICELL8-chip (Takara) with the MultiSample NanoDispenser (Takara) ...
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bioRxiv - Neuroscience 2021Quote: ... the primary antibody rabbit anti-dsRed (Takara Bio, Cat# 632496, 1:500) and the secondary antibody goat anti-rabbit ...
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bioRxiv - Cell Biology 2021Quote: Purified proteins or polymers were diluted using 1× PBS (Takara Bio, T900), and loaded into custom-made microneedles prepared with the P1000IVF micropipette puller (Sutter Instrument) ...
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bioRxiv - Microbiology 2020Quote: Total RNA from 1×106 K562 cells were extracted using Trizol (Takara) and purified with Qiagen RNAeasy mini kit (Qiagen) ...
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bioRxiv - Microbiology 2021Quote: ... then primary staining for mCherry (rabbit anti-DsRed, 632393, Clontech, 1:300) and secondary staining (goat anti-rabbit Ig Cy3 ...
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bioRxiv - Cell Biology 2022Quote: ... and a pAb anti-GFP (632592, WB: 1/1000) was from Takara. All secondary Abs for immunofluorescence (IF ...
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bioRxiv - Neuroscience 2020Quote: ... 1:1,000 for rabbit anti-DsRed (Takara Bio USA #632496, RRID: AB_10013483)) were applied to the samples at 4°C for 2 days ...
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bioRxiv - Cancer Biology 2021Quote: ... relative cell mass was assessed using WST-1 Cell Proliferation Reagent (Clontech) per manufacturer’s instruction ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit anti-DSRed antibody (Living Colours; Clontech; Cat# 632496, dilution 1:300), rabbit anti-red fluorescent protein (RFP ...
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bioRxiv - Microbiology 2019Quote: ... and 0.025 U µl−1 of Taq polymerase (Takara Biotechnology Ltd., Japan). Different dilutions (undiluted to 1:100 in PCR-grade water ...
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bioRxiv - Neuroscience 2021Quote: ... and/or anti-DsRed (host: rabbit, 1/500, #632496 Takara Bio Clontech). Slices were then rinsed three times in PBS (10 min each ...
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bioRxiv - Neuroscience 2021Quote: ... and/or anti-DsRed (host: rabbit, 1/500, #632496 Takara Bio Clontech). Slices were then rinsed three times in PBS (10 min each ...
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bioRxiv - Microbiology 2020Quote: M.tb H37Ra were pelleted and re-suspended in 1 mLTrizol Reagent (TAKARA), and then homogenized using Lysing Matrix B (MP Bio Medical ...
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bioRxiv - Biochemistry 2021Quote: ... Expression of CLPTM1L-mEGFP was induced by 1 µg/mL doxycycline (TaKaRa) and maintained in DMEM supplemented with Tet System Approved FBS (631107 ...
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bioRxiv - Microbiology 2021Quote: ... and incubated with Rabbit anti-ZsGreen (Clontech, Cat. No. 632474, 1:10,000) for 15-18hrs at room temperature ...
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bioRxiv - Genomics 2019Quote: ... 1-U/μL SMARTer Kit RNase Inhibitor (40 U/μL; Clontech, 634936), 10-U/μL SMARTScribe™ Reverse Transcriptase (100 U/μL ...
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bioRxiv - Neuroscience 2022Quote: ... The following antibodies were used: DS Red (1:1000, Rabbit, Takara-Clontech), ChAT (1:300 ...
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bioRxiv - Neuroscience 2022Quote: ... The following antibodies were used: DS Red (1:1000, Rabbit, Takara-Clontech), ChAT (1:300 ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with mouse anti-GFP (1:500, Takara Bio, 632380), rabbit anti-mCherry (1:500 ...
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bioRxiv - Microbiology 2022Quote: ... the cells were treated with or without 1 μM rapalog (Takara Bio) for one hour prior to fixation ...
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bioRxiv - Neuroscience 2022Quote: ... The following antibodies were used: rabbit DsRed (1:3000, Takara Bio, 632496), rabbit β3-tubulin (1:3000 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then treated with 1 µM Shield1 (Takara Bio Cat#632189) 5 days post-BFP sort then harvested for flow cytometry and Western blot analysis 3 days post-Shield1 treatment ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was incubated with 1 μL of S1 nuclease (Takara, Okasa, Japan) for 4 h at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 1 ml Lenti-X Concentrator (Takara Bio; Cat. No. 631231), mixed thoroughly ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 µL of premix WST-1 cell proliferation assay (Takara Bio, Inc.) was added to each well and the plate was incubated for an additional 2 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... Ten ng of cDNA were mixed with 1 x ExTaq buffer (TAKARA), 2.5 mM of each dNTP 2 μl ...
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bioRxiv - Developmental Biology 2023Quote: ... and a dilution 1:000 of the anti-DsRed antibody (Takara; 632496) for mCherry were used ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... residues 1–1235) were cloned into the mammalian overexpression vector pcDNA3.1 (Clontech) together with a green fluorescent protein and a twin strep-II-tag sequence for affinity purification at the C-terminus ...
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bioRxiv - Neuroscience 2023Quote: ... anti-GFP (Clontech, JL-8, 1:10,000 in 5% non-fat milk) and anti-GAPDH (Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... IHC was conducted using rabbit primary antibody dsRed (1:500, #632496, Takara) to label TdT+ cells ...