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Citations for Takara Bio :
1351 - 1400 of 2511 citations for 6 Chloro 4 hydroxy 2 methyl 2H thieno 2 3 e 1 2 thiazine 3 carboxylic acid methyl ester 1 1 dioxide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: Libraries were prepared using 1ng of purified cDNA according to the ICELL8® 3’ DE instruction manual (Takara Bio) using the Nextera Primer P5 (ICELL8® 3’ DE Kit ...
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bioRxiv - Immunology 2022Quote: The sequence of anti-IFNγ was cloned from XMG1.2 hybridoma (ATCC) using SMARTer RACE 5’/3’ kit (Takara Bio). The 6xHis tagged anti-Dsg ...
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bioRxiv - Bioengineering 2022Quote: About 130 ml of clarified cell suspension was combined with 3 mL of TALON® resin (Takara Bio 635503) previously equilibrated in lysis buffer and rocked at 4°C for 1 h ...
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bioRxiv - Molecular Biology 2021Quote: The s2m sequence or control scrambled sequence of s2m (s2m_scr) was inserted into the 3’ UTR of GFP in H6P plasmid using In-Fusion Cloning kit (TaKaRa) and verified by sequencing ...
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bioRxiv - Microbiology 2021Quote: PDGFRβ-targeted sgRNA (5’-CCGGTGAGAGCCACCCTGACAGTG-3’) was cloned into the pGuide-it-ZsGreen1 vector (Takara, Biomedical Technology, Beijing, China). This plasmid could simultaneously express Cas9 ...
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bioRxiv - Plant Biology 2019Quote: ... SmCOP1 and SmHY5 respectively into the yeast strain AH109 as instructions for Matchmaker GAL4 Two-Hybrid Systems 3 (Clontech). Yeast Two-Hybrid analysis were performed following Yeast Protocols Handbook (Clontech) ...
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bioRxiv - Biophysics 2019Quote: ... and the products were further purified in 3% agarose-TBE gels and subsequently reisolated using gel-purification kits (Clontech).
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bioRxiv - Evolutionary Biology 2021Quote: ... The template plasmid for the mCherry-targeting ssDNA was constructed with 1.5 kb long 5’ and 3’ arms amplified from the C57BL/6N genome using PrimeSTAR GXL DNA Polymerase (TaKaRa), the upstream genome sequence of the stop codon of Rtl5 and downstream of the predictive cut site by Cas9 ...
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bioRxiv - Microbiology 2020Quote: 5’-RACE analysis was performed using SMARTer RACE 5’/3’ kit and In-Fusion HD Cloning kit according to the manufacturer’s instructions with slight modifications (Clontech). 5’-RACE ready cDNA was synthesized from purified mRNA (TG ...
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bioRxiv - Immunology 2021Quote: ... Cells were harvested after 3 d and AAV6 was purified with a filtration-based kit (Takara AAVpro Purification Kit) per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant with the soluble His6-UppH was loaded on to a 3 ml TALON Metal Affinity Resin (Clontech) equilibrated in binding buffer (50 mM Na2HPO4 ...
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bioRxiv - Cell Biology 2022Quote: ... then imaged after overnight expression and ∼3 hour incubation with 500 nM A/C heterodimerizer linker drug (Takara, 635055) or 100% ethanol ...
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bioRxiv - Molecular Biology 2022Quote: ... Tagged proteins were bound to 6 (=3 mL bed volume) or 3 mL (=1.5 mL bed volume) pre-equilibrated Talon SuperFlow Metal Affinity Resin from TaKaRa (LarA wt ...
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bioRxiv - Immunology 2022Quote: ... Recombinant adenovirus vaccines were produced using the Adeno-X™ Adenoviral System 3 according to the manufacturer’s manual (Takara Korea Biomedical Inc. ...
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bioRxiv - Microbiology 2022Quote: ... and the 3’ flanking region) and the linearized pHIS906 were fused using the In-Fusion enzyme (TAKARA, Shiga, Japan) to create the pHIS3-AWP1 plasmid ...
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bioRxiv - Cell Biology 2024Quote: ... filtered through a 0.45 µm polyethersulfone filter and used directly or concentrated 3-fold with Lenti-X Concentrator (Clontech).
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bioRxiv - Neuroscience 2024Quote: ... AAV was purified 3–5 days after transfection using AAVpro Purification Kit Midi or Maxi (Takara Bio, Shiga, Japan). The viral concentration was measured by qRT-PCR.
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Retrovirus-derived RTL9 plays an important role in innate antifungal immunity in the eutherian brainbioRxiv - Evolutionary Biology 2023Quote: ... The plasmid for mCherry insertion was constructed with 1.5 kb long 5’ and 3’ arms amplified from the C57BL/6N genome using PrimeSTAR GXL DNA Polymerase (TaKaRa). The 5’ arm is the genomic sequence upstream of the stop codon of Rtl9 and the 3’ arm is downstream of the predictive Cas9 cut site ...
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bioRxiv - Cancer Biology 2023Quote: ... Reverse transcription of mRNA was performed using 3-5 μg RNA with RNA to cDNA EcoDry Premix (Takara, #639549). For real-time PCR analysis ...
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bioRxiv - Plant Biology 2023Quote: ... and SD4 (-Trp-Leu-His-Ade) media at 30℃ for 3–5 d according to the manufacturer’s manual (Clontech).
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bioRxiv - Biophysics 2024Quote: ... to reduce the detergent concentration and incubated for 3 h with 10 mL Talon metal affinity resin (Takara Biotech) pre-equilibrated with buffer S supplemented with 0.1% β-DDM and 0.02% CHS ...
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bioRxiv - Immunology 2024Quote: ... sfGFP-3×P3-DsRed and homology arms were PCR-amplified using PrimeSTAR® Max DNA Polymerase (Takara Bio, R045). Primers for PCR were designed using the NEBuilder Assembly Tool ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-P-cadherin (TAKARA Cat#M127, 1:100 for IF); mouse anti-Rac1 (Abcam Cat#ab33186 ...
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bioRxiv - Microbiology 2021Quote: ... and 1 ng of a luciferase (luc2) containing plasmid (pGL4.26, Clontech) was added to buffer AL prior to lysis ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 1 unit of TaKaRa LA Taq DNA polymerase (Takara Bio, Japan), 0.4 µM each of forward and reverse primers ...
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bioRxiv - Neuroscience 2021Quote: ... and/or rabbit anti-dsRed (1:1000, Takara Bio Cat# 632475) in antibody dilution buffer (PBS ...
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bioRxiv - Neuroscience 2021Quote: ... anti-human GFAP (mouse IgG1, 1:2000, Takara Bio, Shiga, Japan), anti-CNPase (mouse IgG1 ...
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bioRxiv - Developmental Biology 2019Quote: ... 5μl of RT mix containing 1× SMART First Strand Buffer (Clontech), 2 mM dithiothreitol (Clontech) ...
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bioRxiv - Developmental Biology 2019Quote: ... according to Clontech manual PT3460-1 (now part of Takara Bio). Cre recombinase was inactivated by incubating the reaction at 70 °C for 10 minutes ...
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bioRxiv - Neuroscience 2019Quote: ... then incubated in rabbit anti-ds-Red (1:1500; Clontech: #632496) overnight at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... monoclonal mouse anti-STEM121 (1:500; Y40410, Takara, Mountain View, CA), polyclonal rabbit anti-GFAP (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... Stem123 (AB-123-U-050, 1:1000 dilution; Takara Bio Inc.), TBR1 (ab183032 ...
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bioRxiv - Neuroscience 2021Quote: ... Stem121 (AB-121-U-050, 1:1000 dilution; Takara Bio Inc.), Stem123 (AB-123-U-050 ...
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bioRxiv - Neuroscience 2021Quote: ... anti-DsRed (rabbit polyclonal, 1:500, Clontech, Cat# 632496, RRID: AB_10013483); anti-EGFR (goat polyclonal,1:100 ...
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bioRxiv - Neuroscience 2021Quote: ... and mCherry (1:200, Takara Bio USA Inc./Clontech Laboratories, 632543). Retinae were then washed in 0.1% PBST for 3 × 10 minutes at room temperature and incubated with goat-anti mouse Cy3 (1:250 ...
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bioRxiv - Neuroscience 2020Quote: ... and rabbit polyclonal anti-Ds-red (1:200; Clontech, Cat#632496). Secondary antibodies included Cy2 goat anti-mouse (1:400 ...
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bioRxiv - Plant Biology 2021Quote: ... membranes were incubated in anti-GFP (Takara Bio, 1:5000 dilution) antibodies followed by a 2 h incubation in secondary goat anti-mouse-HRP (1:10,000 dilution ...
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bioRxiv - Plant Biology 2021Quote: ... immunoblot with an anti-GFP antibody (1:5000 dilution, Takara Bio) followed by and detection were performed as described (Basu ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated with primary antibodies: mouse anti-GFP (1:2000; Clontech), mouse anti-FLAG (1:1000 ...
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bioRxiv - Neuroscience 2019Quote: ... and rabbit αdsRed (1:500, #632496, ClonTech, Mountain View, CA, USA) in blocking solution ...
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bioRxiv - Developmental Biology 2019Quote: ... rabbit anti-RFP (1:200, 632496, Clontech, Mountain View, CA, USA), mouse anti-β-galactosidase (1:1000 ...
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bioRxiv - Neuroscience 2019Quote: ... rabbit anti-DsRed (1:200; 632496, Clontech, Mountain View, CA, USA), mouse anti-rat CD2 (1:200 ...
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bioRxiv - Cell Biology 2020Quote: ... 1 ug total RNA and PrimeScript RT Master Mix (Takara, Japan) was used for reverse transcription in a SimpliAmp Thermal Cycler (Life technologies ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-dsRed (TaKaRa Bio, cat. no. 632496, 1:250 (57), chicken anti-GFP (Aves ...
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bioRxiv - Plant Biology 2020Quote: pAS6 (Figure 1) carries the YFP coding region of pEYFP (Clontech) interrupted with the synthetic ...
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bioRxiv - Genetics 2021Quote: ... washed and immunostained with rabbit Anti-dsRed (632496, Clontech; 1:1000) and/or mouse Anti-EGFP (DHSB 12A6 ...
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bioRxiv - Cell Biology 2019Quote: The following plasmids were obtained commercially: pEGFP-C2 (Clontech #6083-1), pcDNA-EGFP-ELYS-polyA (Addgene #59746 ...
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bioRxiv - Pathology 2021Quote: ... then loaded onto hand-packed 1 ml TALON (635507, Takara Bio) columns recirculating overnight at 1.5 ml/min ...
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bioRxiv - Neuroscience 2019Quote: ... and rabbit anti-DsRed (1:1000, Takara Bio, Mountainview, CA, USA) primary antibodies was performed in blocking solution overnight at 4°C ...
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bioRxiv - Genetics 2021Quote: ... probed with anti-GFP (Takara Bio Clontech, #632380, mouse, 1:5000) and anti-Tubulin (Sigma ...