Labshake search
Citations for Takara Bio :
1001 - 1050 of 1523 citations for Human TGF beta 3 TGFB3 qPCR Primer Pair since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2022Quote: The coding sequence of human PAC (NP_060722) previously subcloned into pIRES2-EGFP vector (Clontech) using XhoI and EcoRI restriction enzyme sites9 was used for whole-cell patch clamp recording ...
-
bioRxiv - Neuroscience 2024Quote: Human embryonic kidney cells (Lenti-X 293T Cell line from Takara. Cat no. 632180) were grown in DMEM/F12 media (GIBCO ...
-
bioRxiv - Molecular Biology 2024Quote: The human embryonic kidney (HEK) 293T cell line was purchased from Takara (Cat# 632180) and cultured in a humidified 95% air / 5% CO2 incubator at 37°C in a standard Dulbecco’s modified Eagle’s medium (DMEM ...
-
bioRxiv - Immunology 2023Quote: Immunocult-stimulated human CD3+ T cells were retrovirally transduced on Retronectin-coated plates (TaKaRa). 500 µL of cells per well at 0.5 × 106 cells/mL were supplemented with 0.5 mL retroviral supernatant and centrifuged for 90 minutes at 900 g at 32°C ...
-
bioRxiv - Neuroscience 2023Quote: ... GFP-tagged human tauKQ and tauP301L were expressed from the pEGFP-C1 plasmid (Clontech). Expression of mApple or GFP in cultured neurons was done using pGW1 mApple and pEGFP-C1 plasmids ...
-
bioRxiv - Systems Biology 2023Quote: ... before library preparation using the SMARTer Human TCR α/β Profiling Kit (Takara Bio). In brief ...
-
bioRxiv - Cancer Biology 2023Quote: Human DHRS7 was expressed as a GFP fusion using the pEGFP-N2 vector (Clontech) generated previously [5] ...
-
bioRxiv - Synthetic Biology 2023Quote: Human Embryonic Kidney (HEK) 293 cells (ATCC, CRL-1573) and HEK293T-LentiX (Takara Biosciences) were cultured in standard culture conditions (37ºC and 5% CO2 ...
-
bioRxiv - Microbiology 2024Quote: Human embryonic kidney 293T Lenti-X cells (‘293T’) (Cat#: 632180) were purchased from Clontech/Takara Bio (Mountain View ...
-
bioRxiv - Neuroscience 2024Quote: Human embryonic kidney cells (Lenti-X 293T Cell line from Takara. Cat no. 632180) were grown in DMEM/F12 media (GIBCO ...
-
bioRxiv - Cell Biology 2024Quote: ... Human CXCR4 was cloned into the pAcGFPm-N1 plasmid (Clontech Laboratories, Palo Alto, CA), as described (20).
-
bioRxiv - Molecular Biology 2021Quote: ... The resulting cDNA was diluted 5-20 fold and analyzed by real-time qPCR using the SYBR Green master mix (Takara bio) and 400 nanomolar each of the following primers ...
-
bioRxiv - Microbiology 2021Quote: ... cDNA was used as a template for SYBR Green-based qPCR using TB Green Premix Ex Taq™ II Reagent (Clontech) and an ABI Quant 5 thermocycler (Applied Biosystems) ...
-
bioRxiv - Microbiology 2020Quote: ... qPCR experiments were performed in 72-well PCR plates using SYBR® Premix Ex TaqTMII-Tli RNaseH Plus (Takara, Dalian, China) in a 20-μL final reaction volume ...
-
bioRxiv - Plant Biology 2021Quote: The expression of DGAT1s in the transgenic Arabidopsis lines was analyzed by quantitative RT–PCR (qPCR) on a Thermal Cycler Dice Read Real-Time System (Takara, Japan) with the SYBR Green Premix EX Taq GC™ (Takara ...
-
bioRxiv - Microbiology 2021Quote: ... The copy number of viral RNA was standardized with a SARS-CoV-2 direct detection RT-qPCR kit (Takara, cat# RC300A). The fluorescent signal was acquired using a QuantStudio 3 Real-Time PCR system (Thermo Fisher Scientific) ...
-
bioRxiv - Immunology 2021Quote: ... and the reverse transcription products were amplified using the Agilent-Strata gene MxReal-Time qPCR system with a PrimeScript™ RT Reagent Kit (Takara). Reverse transcription products were amplified using an Agilent-Strata gene MxReal-Time qPCR system with TB Green® Premix Ex Taq™ II (Tli RNaseH Plus ...
-
bioRxiv - Physiology 2022Quote: ... Synthesized cDNA samples were used as templates for quantitative PCR using THUNDERBIRD SYBR qPCR Mix (TOYOBO) on a Thermal Cycler Dice Real Time System (Takara Bio). The amount of target RNA was normalized to the endogenous control ribosomal protein 49 gene (rp49 ...
-
bioRxiv - Molecular Biology 2021Quote: ... One ng of DNA was used for real-time qPCR analysis using Premix Ex Taq DNA polymerase (Takara, cat. no. RR039W) on ABI Fast 7500 (Applied Biosystems® ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... and used in qPCR with Takara SYBR Premix Ex Taq (Tli RNaseH Plus) (Takara Bio USA Inc., Mountain View, CA, USA) and LC480 (Roche).
-
bioRxiv - Microbiology 2021Quote: ... The copy number of viral RNA was standardized with a SARS-CoV-2 direct detection RT-qPCR kit (Takara, Cat# RC300A). The fluorescent signal was acquired using a QuantStudio 3 Real-Time PCR system (Thermo Fisher Scientific) ...
-
bioRxiv - Genomics 2022Quote: The expression of each erbb3b gene and the two housekeeping genes (rpl7 and rpl32) was obtained by RT-qPCR at 65 °C (PrimeScript transcriptase, Takara, SYBRgreen) and normalized with the Pfaffle equation (Ståhlberg et al. ...
-
Induction of telomerase in p21-positive cells counteracts capillaries rarefaction in aging mice lungbioRxiv - Molecular Biology 2022Quote: ... The resulting cDNA was diluted 5-20 fold and analyzed by real-time qPCR using the SYBR Green master mix (Takara bio) and 400 nanomolar each of the following primers ...
-
bioRxiv - Zoology 2024Quote: ... RNA was extracted using Isogen-LS (Nippon Gene) and RT-qPCR was performed using the One-Step PrimeScript RT-PCR Kit (Takara Bio) on a 7500 Real-Time RT-PCR System (Applied Biosystems) ...
-
bioRxiv - Physiology 2024Quote: ... Synthesized cDNA samples were used as templates for quantitative PCR using THUNDERBIRD SYBR qPCR Mix (TOYOBO) on a Thermal Cycler Dice Real Time System (Takara Bio). The amount of target RNA was normalized to the endogenous control ribosomal protein 49 gene (rp49 ...
-
bioRxiv - Cell Biology 2024Quote: ... followed by quantitative RT-PCR (qRT–qPCR) using the TB Green® Premix Ex Taq™ (Tli RNaseH Plus) (RR420A, Takara). Data were normalized to GAPDH expression utilizing the 2-ΔΔCT method ...
-
bioRxiv - Microbiology 2023Quote: ... RT-qPCR was performed in a single-step using the One-Step TB Green PrimeScript RT-PCR Kit II (Takara Bio). The HCoV-OC43 nucleocapsid gene was amplified with the following primers ...
-
bioRxiv - Microbiology 2023Quote: ... Real-time PCR was performed using THUNDERBIRD Next SYRB qPCR Mix (TOYOBO) on a real-time PCR system (Thermal Cycler Dice® Real Time System, Takara).
-
bioRxiv - Molecular Biology 2023Quote: Sample preparation for RT-qPCR from primary cultured neurons was performed using the RNeasy Mini Kit and PrimeScript RT Master Mix (Takara Bio). RT-qPCR was performed using KOD SYBR qPCR Mix (TOYOBO ...
-
bioRxiv - Plant Biology 2023Quote: ... qRT-PCR was performed with GeneAce SYBR qPCR Mix α No ROX (Nippon Gene, Tokyo, Japan) in Thermal Cycler Dice Real Time System II (Takara). Each Actin1 genes (PtActin1 and TpActin1 ...
-
bioRxiv - Genetics 2023Quote: ... with minute modifications following the manufacturer’s instructions on the use of Premix Ex Taq™ (Probe qPCR) mix (TaKaRa, Japan, RR390L). Briefly ...
-
bioRxiv - Neuroscience 2024Quote: ... Titers were obtained on freeze-thawed aliquots using the qPCR-based TaKaRa lentivirus titration kit (#631235, Takara Bio, Kusatsu, Shiga, Japan). Minimum titers for all in vivo experiments was 1×1011 genome copies/mL ...
-
bioRxiv - Microbiology 2024Quote: RT-qPCR was performed using total RNA as a template after removing contaminating genomic DNA using gDNA Eraser (TaKaRa, Shiga, Japan). The reaction was carried out using 100 ng of total RNA with the Thermal Cycler Dice Real-Time System and GoTaq® 1-step RT-qPCR Systems (Promega) ...
-
bioRxiv - Microbiology 2024Quote: ... and yapB (50.5 min) were quantified by real-time qPCR using TB Green Premix Ex TaqII (Tli RNaseH Plus) (TaKaRa, Shiga, Japan) and the following primers ...
-
bioRxiv - Cancer Biology 2024Quote: ... Quantitative real-time polymerase chain reaction (qPCR) was carried out using the TB GreenTM Premix Ex TaqTM II (Takara Bio, USA) according to the manufacturer’s protocol for a total reaction volume of 20 μl ...
-
bioRxiv - Evolutionary Biology 2021Quote: NifS genes (Pelo6206 and Pelo13211) were amplified from cDNA using specific primers (S4 Table) and PrimeSTAR® Max DNA Polymerase (Takara Bio Inc.) premix ...
-
bioRxiv - Plant Biology 2020Quote: ... Then the first-strand cDNA synthesis was primed with an oligo (dT) primer by using a PrimeScript™ RT reagent Kit with gDNA Eraser (Perfect Real Time) (Takara, Dalian, China) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2020Quote: ... RAD51 was PCR amplified from pGAT3-RAD51 (Davies et al. 2007) with primers that included Mfel and XhoI sites and then ligated into pGADT7 (Clontech, Mountain View, CA) digested with EcoRI/XhoI ...
-
bioRxiv - Microbiology 2020Quote: The coding sequence for MrvR was PCR amplified from 10/84 genomic DNA using primers optimized for subsequent cloning into the four supplied BIC Brevibacillus expression system pBIC plasmids (Takara cat. # HB300/HB310) in order to generate secreted ...
-
bioRxiv - Developmental Biology 2020Quote: ... coding sequences were amplified by PCR from zebrafish cDNA using specific primers (Table S1) and subcloned into mCherry-C1 vector (Clontech, Mountain View, CA). hVPS4A in pEGFP-C1 was previously described in Elia et ...
-
bioRxiv - Plant Biology 2021Quote: ... Reverse transcription was then carried out using total RNA and oligo-dT primers to synthesize the first strand cDNA using the PrimeScript One-Step RT-PCR Kit (Ver.2, Takara Biotechnology, Dalian, China) according to the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2022Quote: ... were amplified using a set of primers with Gateway attB sites (supplementary Table S2) and cloned into the pABAi vector (Cat. No. 630491, Takara Bio USA, Inc.). The MaMYBPA1 and MaMYBPA2 coding sequences were cloned into the pGADT7-GW vector (Clontech ...
-
Membrane-dependent actin polymerization mediated by the Legionella pneumophila effector protein MavHbioRxiv - Molecular Biology 2023Quote: ... Mutations of MavH were introduced by in vitro site-directed mutagenesis using specific primers containing the defined base changes and PrimeSTAR® Max DNA Polymerase (Takara Bio, Inc.) premix ...
-
bioRxiv - Plant Biology 2022Quote: ... the LNK2 promoter was cloned from just before the start of the upstream gene through 142 bases into exon 4 from genomic DNA using primers pDAN1018 and pDAN1019 (Table S2) and inserted into pB7-HFC PmeI/BglII digest and In-Fusion HD cloning (Clontech, Mountain View, California) to generate pB7-LNK2p::LNK2-HFC ...
-
bioRxiv - Plant Biology 2022Quote: ... 2098 bp of the TOC1 promoter was cloned using primers pDAN2735/pDAN2736 (Table S2) and inserted via In-Fusion HD cloning (Clontech, Mountain View, California) into the pGreenII 0800-LUC plasmid (Hellens et al. ...
-
bioRxiv - Plant Biology 2022Quote: ... RVE8 was cloned from genomic DNA without the stop codon using primers pDAN1127 and pDAN1128 (Table S2) and cloned into NotI/AscI-digested pENTR-MCS through In-Fusion HD cloning (Clontech, Mountain View, California). pENTR-RVE8-no stop was then recombined using LR Clonase (Thermo Fisher Scientific ...
-
bioRxiv - Plant Biology 2022Quote: ... the gene of interest was cloned from its pENTR-STOP template using primers pDAN2349/pDAN2350 (Table S2) and recombined into pGADT7 digested with EcoRI using In-Fusion HD cloning (Clontech, Mountain View, California). For cloning into pGBKT7 ...
-
bioRxiv - Neuroscience 2023Quote: ... total RNA was isolated by standard procedure from 20-30 heads of homozygous Aprt5 flies and reverse transcribed using oligo d(T) primers (PrimeScript RT Reagent Kit, Takara Bio, Kyoto, Japan). At least 750 ng of the first strand cDNA was amplified by PCR in 20 μl of reaction mixture using PrimeStar Max DNA polymerase (Takara Bio ...
-
bioRxiv - Genetics 2024Quote: Two μg of total RNA was reverse transcribed into cDNA templates using RNA to cDNA EcoDry™ Premix kit including both random hexamer and oligo(dT)18 primers (Takara Bio, 639548). KAPA SYBR® FAST qPCR Master Mix (2X ...
-
bioRxiv - Biochemistry 2024Quote: ... Sub-library specific primers were then used to amplify 1 ul of cDNA template for 24 cycles using Advantage 2 PCR Mix (Takara Bio, Cat. 639206). Finally ...