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Citations for Takara Bio :
851 - 900 of 2435 citations for Human Chemokine C X C Motif Receptor 5 CXCR5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Lentivirus was made by transfecting Lenti-X 293T cells (Clontech 632180) with CTL plasmids plus psPAX2 and VSV-G packaging plasmids using Lipofectamine 2000 (Invitrogen 11668019) ...
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bioRxiv - Cell Biology 2022Quote: ... the presence of lentivirus was confirmed using Lenti-X GoStix (TaKaRa). Viral supernatants were supplemented with a mixture of 60% DMEM/40% FBS and stored at 4ºC if to be used within the next 48 hours or frozen at -80ºC until use ...
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bioRxiv - Neuroscience 2023Quote: ... 1 million Lenti-X HEK293T cells (Takara Bio, cat. no. 632180) were seeded in 2 mL DMEM (Gibco ...
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bioRxiv - Microbiology 2023Quote: pZIP transfer vectors were packaged in Lenti-X 293T cells (Takara) using molar ratios of 0.2 transfer vector to 0.51 psPax2 to 0.29 pMD2.G ...
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bioRxiv - Cell Biology 2023Quote: ... Viruses were titered using the Adeno-X qPCR Titration kit (Clontech), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Lentiviruses were made by transfecting Lenti-X 293T cells (Takara 632180) overnight with polyethylenimine (PEI) ...
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bioRxiv - Cancer Biology 2023Quote: ... Viral particles were concentrated using Lenti-X Concentrator (Takara Bio, #631232), and cells were transduced at a high multiplicity of infection with different polybrene concentrations (8ug/mL-30ug/mL).
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bioRxiv - Cell Biology 2023Quote: ... the Lenti-X embryonic kidney 293T cell line (Clontech, no. 632180) in DMEM (4.5 g/L D-glucose) ...
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bioRxiv - Molecular Biology 2023Quote: ... Relative titer was estimated by Lenti-X GoStix Plus (Takara #631280) and was similar for all viral constructs.
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bioRxiv - Cell Biology 2023Quote: ... 6-well plates of 70% confluent Lenti-X 293T cells (Clontech) were transfected with 1.5 μg transfer vector ...
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bioRxiv - Cancer Biology 2023Quote: ... were packaged in Lenti-X 293T Cells (Takara Bio, Cat. 632180) by transient transfection ...
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bioRxiv - Cell Biology 2024Quote: ... The virus titer was determined using Lenti-X GoStix Plus (Clontech). To transduce hTCEpi cells ...
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bioRxiv - Developmental Biology 2024Quote: ... Foamy virus was produced using Lenti-X 293T (Takara Bio, Kusatsu) generated as previously described and frozen at -80°C for long term storage42 ...
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bioRxiv - Genetics 2024Quote: ... The lentivirus was concentrated using the Lenti-X concentrator (Takara Bio). The viral titer was determined by performing lentiviral transductions at varying concentrations in a 48-well plate format ...
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bioRxiv - Genomics 2024Quote: ... media was aspirated and concentrated using Lenti-X Concentrator (TaKaRa #631231) following the manufacturer’s recommended protocol ...
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bioRxiv - Immunology 2024Quote: ... Lentivirus was produced by transfecting 95 % confluent Lenti-X cells (Takara) grown in wells of 6-well plates with 1000 ng psPAX2 (Addgene plasmid #12260 ...
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bioRxiv - Immunology 2024Quote: ... lentivirus was precipitated from the supernatant using Lenti-X Concentrator (Takara) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... with optional concentrating lentivirus using Takara’s Lenti-X Concentrator (Takara 631231). Infection of PC3-AR-DTX3LKO cells were carried out in the growth medium (RPMI 1640 + 5% FBS + 1% Penicillin-Streptomycin solution ...
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bioRxiv - Physiology 2024Quote: ... Virus concentration was determined using Lenti-X GoStix Plus (Takara Bio) after one freeze-thaw cycle to be consistent with experimental conditions.
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bioRxiv - Immunology 2024Quote: ... lentivirus was collected and concentrated using Lenti-X Concentrator (Clontech, #631231) overnight at 4°C followed by centrifugation according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... and lentiviral particles were concentrated using the Lenti-X Concentrator (Takara) as per the instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... into HEK293FT or its derivative Lenti-X 293T cells (632180, Takara). Media was replaced after 24 hours and the viral supernatant was collected through filtration (0.45 µm pore ...
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bioRxiv - Cancer Biology 2024Quote: ... The plasmid libraries were then transfected into Lenti-X cells (Takara) to produce lentiviral pools ...
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bioRxiv - Cancer Biology 2024Quote: ... The Mir-X miRNA First-Strand Synthesis Kit (TAKARA, Shiga, Japan) was used to convert RNAs into complementary DNA (cDNA) ...
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bioRxiv - Immunology 2024Quote: ... Viruses were concentrated with the Lenti-X Concentrator reagent (Clontech, #631232). For viral transduction ...
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bioRxiv - Genetics 2023Quote: ... GoStix Value (GV) quantified by the Lenti-X GoStix App (Takara) was used to scale the titer of each block to be the same ...
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bioRxiv - Biophysics 2023Quote: ... Some viruses were concentrated using Lenti-X Concentrator (Takara Cat. # 631232) following vendor protocols and were concentrated 1/10 ...
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bioRxiv - Immunology 2022Quote: 5’ and 3’ RACE was performed with SMARTer RACE 5’/3’ Kit (Takara) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... 5′ RACE was performed using the SMARTer RACE 5/3 kit (Takara Bio) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Human total RNA from different normal tissues from Clontech (Human Total RNA Master Panel II,Cat# ...
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bioRxiv - Cell Biology 2019Quote: ... and human BaxS184V was cloned in pEGFP-C1 (Clontech). Bax/Bak DKO MEFs were grown in DMEM supplemented with 10% FBS ...
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bioRxiv - Biochemistry 2019Quote: ... Human colon cDNA purchased from Clontech (Palo Alto, CA) was used as the template ...
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bioRxiv - Neuroscience 2020Quote: ... anti-human nuclei (STEM101; Takara, Y40400, IgG1, 1:100) and anti-human NOGOA (Santa Cruz Biotechnology ...
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bioRxiv - Developmental Biology 2021Quote: ... and human-specific GFAP marker STEM123 (TaKaRa, cat. Y40420). As general astrocyte markers GFAP (DAKO ...
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bioRxiv - Cancer Biology 2021Quote: ... Normal human stomach tissue RNAs were purchased from TaKaRa Bio (Shiga ...
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bioRxiv - Immunology 2019Quote: ... and Retronectin (Recombinant Human Fibronectin) was purchased from Takara Bio (Saint-Germain-en-Laye ...
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bioRxiv - Neuroscience 2020Quote: Human induced pluripotent stem cells (iPSCs) (ChiPSC18, Takara Bioscience) were reprogrammed using a protocol for midbrain dopaminergic neurons adapted from Kirkeby et ...
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bioRxiv - Cell Biology 2020Quote: Human telomerase-immortalized retinal-pigmented epithelial cells (RPE1; Clontech) either expressing LifeAct-GFP or parental (Vignaud et al. ...
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bioRxiv - Biochemistry 2022Quote: ... human CCDC22 was first cloned into pEGFP-C1 (Clontech) between Sal1 and BamH1 ...
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bioRxiv - Microbiology 2023Quote: ... and the Mate and Plate Universal Human Library (Clontech). This yeast two-hybrid universal library was constructed from human cDNA that has been normalized to remove high copy number cDNAs (overrepresented transcripts ...
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bioRxiv - Genetics 2023Quote: ... Human KISS1 was cloned into pLVX-neo vector (Clontech) for overexpression ...
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bioRxiv - Cell Biology 2023Quote: ... anti-human specific GFAP (hGFAP, TAKARA, Stem123, 1:1000), anti-AQP4 (Cell Signaling ...
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bioRxiv - Cancer Biology 2023Quote: ... Human iPSC line ChiPSC22 (Cellartis/Takara Bio Europe AB) was cultivated in the feeder-free DEF-CS system (Cellartis/Takara Bio Europe ...
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bioRxiv - Synthetic Biology 2020Quote: ... Protein concentrations were measured with a BCA protein assay kit (Takara, Dalian, China).
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bioRxiv - Microbiology 2023Quote: ... Protein concentration was quantified using the BCA protein assay kit (Takara Bio, T9300A). Cell lysates (about 200 µl ...
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Flexible linkers in CaMKII control the balance between activating and inhibitory autophosphorylationbioRxiv - Biophysics 2019Quote: Human CaMKII-α (Uniprot_ID: Q9UQM7) and human CaMKII-β (Uniprot_ID: Q13554) were cloned into the pEGFP-C1 vector backbone (Clontech, Mountain View, CA), after modifying the vector to contain a biotinylation sequence (Avitag ...
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bioRxiv - Neuroscience 2021Quote: ... 5’RACE was carried out using a 5’ Full RACE Core Set (Takara Bio). The first PCR was performed using the single strand cDNAs concatenated by T4 RNA ligase and primers S1 (5’-TTC TAT ACC ATC GTC TAC CCG CTG AGC TTC-3’ ...
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bioRxiv - Molecular Biology 2021Quote: ... 5’ RACE cDNA library was prepared using Clontech SMARTer RACE 5’/3’ Kit (Takara) according to the user manual with the gene-specific primer (S4 Table ...
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bioRxiv - Molecular Biology 2021Quote: The 5′ RACE analysis was conducted using the 5′ -Full RACE Core Set (Takara), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: HEK293T derived Lenti-X™ cells (Takara Bio, Mountain View, CA, USA) and TZM-bl cells (ARP-8129 ...