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Citations for Takara Bio :
651 - 700 of 1268 citations for pVectOZ CAT Transfection control since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10% tetracycline-free fetal bovine serum (FBS, TaKaRa Cat. #631368), 2 mM L-glutamine (Gibco) ...
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bioRxiv - Genetics 2022Quote: ... gDNA was extracted using a Nucleospin Blood Maxi kit (Takara, Cat. #740950) (for CRISPR/Cas9 screening ...
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bioRxiv - Neuroscience 2022Quote: ... we used primary polyclonal rabbit anti-tdTomato from Takara (Cat no. 632496) and secondary anti-rabbit goat Cy3 (Cat no ...
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bioRxiv - Neuroscience 2022Quote: ... cDNA was generated with a PrimeScript RT reagent kit (Takara Cat# RR047A). Quantitative real-time polymerase chain reaction (qRT-PCR ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated with rabbit anti-DsRed (TaKaRa Bio, cat. no. 632496) at 1:1000 and guinea pig anti-Cre (Synaptic Systems ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then treated with 1 µM Shield1 (Takara Bio Cat#632189) 5 days post-BFP sort then harvested for flow cytometry and Western blot analysis 3 days post-Shield1 treatment ...
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bioRxiv - Immunology 2022Quote: ... The SMARTer Mouse TCR a/b Profiling Kit (Takara, Cat. No. 634403) was used to amplify both TCRα and TCRβ sequences ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 1 ml Lenti-X Concentrator (Takara Bio; Cat. No. 631231), mixed thoroughly ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated using NucleoZOL (Takara, Shiga, Japan, Cat. No. 740404.200) following the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2023Quote: Lenti-X 293T cell line (Takara Bio Inc., Japan; cat. no. 632180)
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bioRxiv - Cancer Biology 2023Quote: ... PCR was performed with SeqAmp DNA Polymerase (Takara Bio; cat. no. 63850), and PCR products purified and concentrated with the QIAquick PCR Purification kit (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... Using the PrimeScript first strand cDNA synthesis kit (Takara, cat no- #6110A), the first strand of cDNA was synthesised according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... 7 million Lenti-X HEK-293T cells (Takara Bio, cat. no. 632180) were seeded in 9 mL DMEM (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 6 ml Lenti-X Concentrator (Takara Bio; Cat. No. 631231), mixed thoroughly ...
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bioRxiv - Pathology 2023Quote: ... cDNA was prepared using PrimeScript cDNA synthesis kit (Takara, Cat No: 2680A) according to the manufacturer’s instructions ...
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Autism genes converge on microtubule biology and RNA-binding proteins during excitatory neurogenesisbioRxiv - Systems Biology 2024Quote: ... lentiviruses were produced in Lenti-X 293T cells (Takara Bio, Cat#632180) and lentivirus-containing supernatants were concentrated using Lenti-X concentrator (Takara Bio ...
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bioRxiv - Bioengineering 2023Quote: ... digested in 50 mg/mL Yatalase in PBS (Takara Bio, Cat# T017) for 40 minutes at RT and rinsed 3 times with PBS ...
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bioRxiv - Bioengineering 2023Quote: ... followed by cDNA synthesis using the cDNA Synthesis Kit (Cat# 6110A, Takara). All qRT-PCR primers were ordered from Eton Bioscience ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was added to the Ni-IDA resin (Takara, Cat # 635660) equilibrated with Binding Buffer (50 mM NaPi ...
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bioRxiv - Bioengineering 2023Quote: ... RNA was converted to cDNA using SmartSeq-v4 (Takara, cat no. R400752). The library was prepared using Nextera XT DNA library preparation kit (Illumina ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-dsRed (cross-reacts with TdTomato, 1:1,000, Clontech, Cat#632496), mouse anti-FAT36,7 (1:200) ...
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HOIL-1L deficiency induces cell cycle alteration which causes immaturity of myocyte and fibrogenesisbioRxiv - Cell Biology 2023Quote: ... qPCR was performed using SYBR Green PCR Master Mix (Takara, Cat.# RR820) on a StepOnePlus system (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Lentiviral supernatants were concentrated using Lenti-XTM Concentrator (Takara Bio, Cat # 631232) and then centrifuged at 1500g for 30 minutes at 4°C and supernatant was discarded ...
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bioRxiv - Developmental Biology 2023Quote: ... and resuspended in 10.5ul Clontech Lysis buffer (Takara Bio Inc. Cat # 635013). Lysed samples were stored at -80°C until transferred to the Duke Center for Genomic and Computational Biology Sequencing and Genomic Technologies core facility for cDNA library preparation and next- generation sequencing ...
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bioRxiv - Cell Biology 2023Quote: ... 12.5 µL of Tks Gflex DNA polymerase Low DNA (cat. #R091A, Takara) and 12.2 µL of NFW} was added ...
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bioRxiv - Neuroscience 2024Quote: ... which was synthesized with a PrimeScript reverse transcriptase kit (Cat# RR037A, Takara). RT–PCR was performed using a TB Green TM Premix Ex Taq Kit (Cat# RR820A ...
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bioRxiv - Cell Biology 2024Quote: ... This was then concentrated using Lenti-X concentrator (Clontech Cat. No. 631231). 5 shBLTP2 viral pellets were mixed into an shRNA cocktail in 1ml complete DMEM medium ...
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bioRxiv - Immunology 2024Quote: ... The RNeasy kit and the cDNA Synthesis Kit (Takara, Cat#: 9767, RR047A) was used to extract total RNA and prepare cDNA following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... using a Make Your Own Mate & Plate Library System (Cat. no. 630489, Clontech). The PuBZR1 CDS was introduced into the pGBKT7 vector enclosed in this kit using EcoRI and BamHI sites ...
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bioRxiv - Cell Biology 2020Quote: ... the crude viral solution was concentrated using Lenti-x concentrator (Takara, cat.631232) to a final concentration of 108-109 IFU/ml using the Lenti-x qRT-PCR Titration Kit (Takara ...
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bioRxiv - Molecular Biology 2021Quote: ... Libraries were generated using the DNA SMART ChIP-Seq Kit (Takara, cat# 634865). The libraries were selected from 250–350-bp fragments obtained by E-Gel 2% SizeSelect electrophoresis (Invitrogen ...
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bioRxiv - Cancer Biology 2019Quote: ... Lentiviral titers were estimated using Lenti-X GoStix Plus (Takara Bio, Cat. # 631281). Lentiviral transduction was performed overnight in the presence of 8μg/ml polybrene (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... was purchased from Addgene and the iDimerize™ Inducible Homodimer System (Cat # 635068) was purchased from Clontech Laboratories ...
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bioRxiv - Plant Biology 2021Quote: ... Real-time PCR was performed using a SYBR premix kit (TaKaRa, Cat#RR82LR). The primers used are listed in Supplemental Table 3.
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bioRxiv - Developmental Biology 2021Quote: ... Reverse transcription reactions were conducted using the SMARTer RACE kit (Clontech cat. 634859) or High capacity cDNA Reverse Transcription kit (Applied Biosciences cat ...
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bioRxiv - Plant Biology 2022Quote: ... and inserted into pSun using In-Fusion® cloning (Takara Bio Cat. #121416) according to manufacturers’ instructions.
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bioRxiv - Plant Biology 2022Quote: ... and EGFP was inserted using In-Fusion® cloning (Takara Bio Cat. #121416). For p35S::DAAO-GFP ...
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bioRxiv - Microbiology 2022Quote: ... The filtered supernatant was loaded onto the HisTALON cartridge (Clontech Laboratories, Cat# 635683) column at a speed of 1 mL/min ...
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bioRxiv - Genomics 2022Quote: ... and the ends were blunted using Mighty Cloning Kit (Cat# 6026, Takara Bio). DNA fragments of 4–5 kb were fractionated by agarose gel electrophoresis ...
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TIR signaling promotes the interactions between EDS1/PAD4 and ADR1-L1 and oligomerization of ADR1-L1bioRxiv - Plant Biology 2021Quote: ... Real-time PCR was performed using a SYBR premix kit (TaKaRa, Cat#RR82LR). The primers used are listed in Supplemental Table 1.
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bioRxiv - Cell Biology 2021Quote: ... 200 MOI retrovirus and 5 µg/cm2 RetroNectin reagent (Takara, Cat. No. T100A) were used in the transduction following the manufactory protocol ...
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bioRxiv - Neuroscience 2020Quote: ... lentivirus was concentrated 10x using lenti-X concentrator (Takara Bio USA, Cat# 631231) with the concentrated pellet being resuspended in PBS with 25 mM HEPES ...
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bioRxiv - Molecular Biology 2021Quote: ... using the IQ Supermix with SYBR-Green (Cat. #RR420A; TaKaRa Bio, Shiga, Japan). Gene expression was quantified using the standard 2−ΔΔCt method as described previously ...
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bioRxiv - Microbiology 2022Quote: ... and reverse transcript to cDNA by PrimeScrip RT reagent Kit (Takara cat #RR037A). The corresponding cDNAs were quantified by using Hieff qPCR SYBR Green Master Mix (Yeason cat #11202ES03) ...
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bioRxiv - Genomics 2019Quote: ... The IRES2 fragment was PCR amplified from the pTRE3G-IRES (Cat. # 631174, Clontech) using the primer pairs
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bioRxiv - Genomics 2019Quote: Both fragments were then assembled together with a pTRE3G-IRES (Cat. # 631174, Clontech) backbone ...
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bioRxiv - Neuroscience 2019Quote: scRNA-seq was performed using the SMART-Seq v4 kit (Takara Cat#634894) as described previously6 ...
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bioRxiv - Pathology 2020Quote: ... followed by treatment with 10 U of recombinant DNase I (Cat.#2270A, TaKaRa) to remove residual DNA ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: 50mg of liver sample was taken in 1ml RNAiso (Takara Cat.-9108/9109) and RNA was isolated according to the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2021Quote: Total RNA was extracted from colonic tissues using RNAiso Plus (TaKaRa, Cat# 9109) according to the manufacturer’s protocols ...