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Citations for Takara Bio :
701 - 750 of 1268 citations for pVectOZ CAT Transfection control since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... as two overlapping fragments using high-fidelity Takara LA Taq (Takara, Cat # RR002M). Primer details can be found in Supplementary Table 2 ...
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bioRxiv - Microbiology 2021Quote: ... The filtered supernatant was loaded onto the HisTALON cartridge (Clontech Laboratories, Cat# 635683) column at a speed of 1 mL/min ...
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bioRxiv - Molecular Biology 2021Quote: ... Reactions were then transformed into Stellar™ competent cells (Takara Bio; Cat #: 636766), and plasmids were subsequently isolated using Qiagen mini-prep kits (Qiagen ...
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bioRxiv - Bioengineering 2022Quote: SMARTer RNA Unique Dual Index Kit – 96U Set A (Takara, Cat No 634452)
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bioRxiv - Cell Biology 2022Quote: ... Viral particles were precipitated using Lenti-X maxi-purification kit (Takara, Cat# 631233) based on manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... Total RNA pools from human brain (cat. no. 636530, Takara Bio USA, Inc), small intestine (cat ...
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bioRxiv - Biochemistry 2023Quote: ... Human embryonic kidney (HEK) 293T Lenti-X were obtained from Takara (cat# 632180). These cells transformed with adenovirus type 5 DNA and expresses the SV40 large T antigen ...
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bioRxiv - Microbiology 2023Quote: ... Reverse transcription was performed according to the manufacturer’s protocol (TAKARA, Cat. No. RR047A) using 1 µL of the lysate as reverse transcriptase and TRIzol reagent-isolated 293T total RNA as template ...
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bioRxiv - Genomics 2023Quote: ... 2.5 U Takara Epi Taq HS (Takara, cat. no. R110A, 5 U/µl), 2.5 mM MgCl2 ...
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bioRxiv - Microbiology 2023Quote: ... and reverse transcribed using the TaKaRa PrimeScript RNA Kit (cat# RR036A, Takara, Japan), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from cells using RNAiso Plus Regent (Takara, Cat# 9108) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was isolated from cells using RNAiso Plus Regent (Takara, Cat# 9108) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... the supernatant was harvested and concentrated by Lenti-X-Concentrator (Cat: 631232, Clontech). The virus titer was determined using the Lenti-X™ p24 Rapid Titer Kit (Cat ...
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bioRxiv - Cancer Biology 2022Quote: ... the mRNAs in these cells were extracted using RNAiso Plus (Takara, Cat:9109) and then sequenced by Biomarker Techonologies using Illumina sequencing technology ...
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bioRxiv - Molecular Biology 2022Quote: ... then mixed with universal primer and PrimeSTAR GXL DNA polymerase (Takara, Cat# R050A) and amplified following the program ...
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bioRxiv - Biochemistry 2023Quote: ... Lentivirus was concentrated by adding 333 μL Lenti-X concentrator (Takara, cat# 631231) per mL of viral supernatant ...
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bioRxiv - Cell Biology 2023Quote: ... the cDNAs were inserted into a pEGFP-C1 vector (Clontech cat#6084-1) using the following primers ...
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bioRxiv - Neuroscience 2023Quote: ... DCX: doublecortin (C-18) goat polyclonal IgG (1:200, Takara Bio, Cat# 632496). NeuN ...
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bioRxiv - Neuroscience 2023Quote: pTetO-ZsGreen (pTetO-ZsG) was obtained from Clontech (pmRi-ZsGreen; Cat. No. 631121) and all other pTetO promoters are copies of the PTet-14 promoter (Urlinger et al. ...
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bioRxiv - Neuroscience 2023Quote: scRNA-seq was performed using the SMART-Seq v4 kit (Takara Cat#634894) as described previously (Tasic et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... we enriched the lentiviral particles with Lent-X Concentrator (Cat# 631231, Clontech, Japan) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... 1 μg/ml anti-hCD28 and 10 μg/ml RetroNectin (Takara, Cat# T100A). PBMCs were loaded in these wells in human T cell media (X-VIVO media ...
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bioRxiv - Cancer Biology 2023Quote: ... Concentrated viruses were titered using the p24-Elisa kit (Takara Bio, cat. 632200) following manufacturer’s protocols ...
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bioRxiv - Neuroscience 2023Quote: ... fixed for 1 h in 4% paraformaldehyde/PBS (TAKARA BIO INC. Cat#T900), and subjected to antibody labeling ...
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bioRxiv - Cell Biology 2024Quote: ... Viruses were purified with AAVpro Purification Kit Maxi (All Serotypes) (Takara, cat# 6666), as per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Flanking att sequences were added through another round of PCR (Takara, cat # R011) and purified ...
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bioRxiv - Neuroscience 2024Quote: The mammalian expression vector pBApo-CMV-Pur (cat. #3421) was purchased from Takara. We used Genescript to insert the 144 amino acid sequence for WT synuclein into the backbone and create 6 mutated strains ...
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bioRxiv - Neuroscience 2024Quote: The total RNA of cells was isolated with RNAiso plus (Cat# 9108, Takara). The concentration and purity of RNA samples were measured using a Nanodrop ND-2000 (Thermo Science ...
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bioRxiv - Cancer Biology 2024Quote: ... sgRNA inserts were PCR amplified using Ex Taq DNA Polymerase (Takara, cat#RR001A) from sufficient genome equivalents of DNA to achieve an average coverage of >200x of the sgRNA library ...
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bioRxiv - Cancer Biology 2023Quote: ... sgRNA inserts were PCR amplified using Ex Taq DNA Polymerase (Takara, cat#RR001A) from sufficient genome equivalents of DNA to achieve an average coverage of >200x of the sgRNA library ...
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bioRxiv - Cancer Biology 2021Quote: ... Reverse-transcription was performed using the PrimeScriptTMRT Master Mix Reagent Kit (Takara, Cat. #RR036A) and quantitative real-time PCR was performed using the SYBR® Premix Ex Taq II Reagent Kit (Takara ...
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bioRxiv - Immunology 2020Quote: Mouse tail DNA was amplified using KAPA Taq PCR Kit (Takara Bio, cat. R040A) and primers Rosa26 WT F ...
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bioRxiv - Immunology 2020Quote: ... cDNA was amplified using using PrimeSTAR HS DNA Polymerase (Takara Bio Inc., cat. R040A) and the primers GFP F AgeI 72 and GP80 R XhoI 64 (Table S1) ...
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bioRxiv - Genomics 2019Quote: ... The chip was sealed with a TE Sealing film (Takara Bio USA, Cat. #640109) and on-chip PCR was performed using a SMARTer ICELL8 Thermal Cycler (Takara Bio USA ...
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bioRxiv - Genomics 2019Quote: ... cells were dispensed into a SMARTer ICELL8 350v chip (Takara Bio USA, Cat. # 640019) at 35 nanoliter per well ...
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bioRxiv - Neuroscience 2020Quote: ... and the virus collected after 72h using Lenti-X-concentrator (Cat. No. 631231, Clontech).
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was applied to 4-mL Talon Metal Affinity Resin (Clontech, cat# 635503). After washing with 30 mL wash buffer containing 20 mM HEPES at pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... The cDNA clone for Cap1 was purchased from Origene (CAT#: MR207594) and subsequently cloned into lentiviral vector pLVX-puro (Clontech, Mountain View, USA). For overexpression of the candidate miRNAs ...
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bioRxiv - Systems Biology 2021Quote: ... The SMARTer Universal Low Input RNA Kit protocol v2 (Cat #634938, Takara Bio USA) was used for cDNA synthesis and library construction ...
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bioRxiv - Cell Biology 2021Quote: ... the media was incubated with ⅓ volume of Lenti-X Concentrator (Takara, Cat No. 631231) at 4°C for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... or the pZsGreen vector (cat. # 632446; Takara Bio USA, Inc., Mountain View, CA, USA). Transformed E ...
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bioRxiv - Plant Biology 2022Quote: ... EGFP was inserted using HindIII and In-Fusion® cloning (Takara Bio Cat. #121416). For pAGO9::EGFP-AGO9 ...
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bioRxiv - Genomics 2022Quote: ... The insert fragment was extracted and treated with Exonuclease III (Cat# 2170, Takara Bio) typically at 23 °C for 3 ...
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bioRxiv - Cancer Biology 2022Quote: ... was inserted using the In-fusion HD cloning kit (Cat. No. 639648, Takara-bio) into the SpeI restriction site in the pPB-LR5 ...
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bioRxiv - Developmental Biology 2021Quote: ... 4.75 μl Dilution buffer (SMARTer Ultra Low Input RNA kit, Clontech; Cat. No. 634820) and 5 μl nuclease-free water on a pre-chilled CoolRack (Biocision ...
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bioRxiv - Developmental Biology 2021Quote: ... Libraries were prepared using Low Input Library Prep Kit (Clontech Laboratories; Cat. No. 634900) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Lentivirus was first concentrated 100x using Lenti-X Concentrator kit from Takara (Cat # 631231). Concentrated lentivirus was aliquoted and stored in −80 before use ...
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bioRxiv - Molecular Biology 2020Quote: ... Primary PCR was performed using EmeraldAmp® GT PCR Master Mix (Takara cat# RR310A) 10 ul ...
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bioRxiv - Molecular Biology 2020Quote: ... Baculoviral titers were determined using a BackPak Baculovirus Rapid Titer kit (Clontech Cat# 631406).
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bioRxiv - Molecular Biology 2020Quote: ... All clonings have been performed using In-Fusion HD Cloning Plus (Takara, Cat#638911) according to manufacturer’s recommendations ...