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Citations for Takara Bio :
801 - 850 of 1268 citations for pVectOZ CAT Transfection control since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... GCN2 coding sequence was subcloned into the lentiviral vector pLVX-IRES-Hygromycin (Clontech, Cat #632185) using an In-Fusion HD Cloning plus kit (TaKaRa ...
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bioRxiv - Cancer Biology 2019Quote: ... Virus was collected after 48 hours and concentrated with Lenti-X Concentrator (Clontech, Cat#631231) (16) ...
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bioRxiv - Neuroscience 2019Quote: ... into the EcoRI and BamHI sites of pLVX-EF1a-IRES-Puro (Clontech, Cat. Nr. 631988). pLVX-EF1a-TS-OPT-FUS-IRES-Puro was cloned by introducing an N-terminal Twin-Strep (TS)-tagged codon optimized FUS cDNA (Gene synthesis by GeneArt ...
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bioRxiv - Neuroscience 2019Quote: ... into the EcoRI and BamHI sites of pLVX-EF1a-IRES-Puro (Clontech, Cat. Nr. 631988).
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bioRxiv - Microbiology 2019Quote: ... RNA was reverse transcribed to cDNA using the PrimeScript RT Reagent Kit (TaKaRa, cat. #RR047A). Then ...
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bioRxiv - Developmental Biology 2020Quote: ... Reverse transcription (RT) was performed using the PrimeScript RT master mix (cat. #RR036A, Takara, Japan). Real-time RT-PCR was performed in a StepOne plus system (ABI StepOne Plus ...
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bioRxiv - Cancer Biology 2020Quote: ... filtered with a 0.45 um filter and concentrated using Lenti-X concentrator (Clontech, Cat#631232) and stored at 80°C.
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bioRxiv - Molecular Biology 2020Quote: ... After incubation with horseradish peroxidase-conjugated secondary antibody (IgG detector, Takara Clontech, Cat# T7122A-1), the antigen was detected using chemiluminescence Western blotting detection reagents (Pierce ECL Western Blotting Substrate ...
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bioRxiv - Neuroscience 2020Quote: ... rDA1m was immunostained using a rabbit anti-RFP antibody (1:1000, Takara, cat. number 632496) followed by a Cy3-conjugated donkey anti-rabbit secondary antibody (1:1000 ...
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bioRxiv - Developmental Biology 2019Quote: ... cDNA was synthesized using SMART-Seq v4 ultra low input RNA kit (Clontech, cat # 634889). An mRNA-seq library was then prepared from each sample using standard Illumina TruSeq protocol ...
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bioRxiv - Physiology 2021Quote: ... Purified AAVs were titered using a qPCR based kit (Cat. No. 6233; Takara Bio, USA). The TA muscle of each mouse received a 5 x 1010 vg of either AAV-GFP or AAV-CAAHR using several small volume (~7-8 μl ...
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bioRxiv - Physiology 2021Quote: ... plasmids were transfected into C2C12 cells using Xfect reagent (Cat. No. 631324; Takara Bio, USA) according to manufacturer instructions.
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bioRxiv - Genomics 2021Quote: ... The contents of the chip were then centrifuged into a collection tube (Takara Cat# 640048) at 1200g for 3 min ...
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bioRxiv - Cell Biology 2021Quote: ... Lentivirus vectors pLVX-Tet3G and pLVX-TRE3G (Cat. 631358 and 631193) were purchased from Takara Bio.
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bioRxiv - Molecular Biology 2020Quote: ... the final volume was increased to 1 mL using SOC medium (cat. no. ST0215, Takara) pre-warmed to 37 °C ...
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bioRxiv - Genomics 2021Quote: ... Library preparations were done using the SMARTer Thruplex DNA-seq kit from Takara (cat# R400674). Library concentration was determined using Qubit (Thermofisher ...
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bioRxiv - Cancer Biology 2020Quote: ... 500ng of total RNA were retrotranscribed using PrimeScript RT Master Mix (Takara Bio, Cat. # RR036A), and subsequent TaqMan assay-based or SYBR Green-based qPCRs were performed using PerfeCTa qPCR ToughMix (QuantaBio ...
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bioRxiv - Developmental Biology 2022Quote: ... Reverse transcription was performed with the PrimeScript High Fidelity RT-PCR Kit (Takara, Cat. R022A). RT-PCR was run on a 7900HT Fast RT-PCR instrument (Applied Biosystems ...
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bioRxiv - Neuroscience 2022Quote: 50 µg of human cerebral cortex lysate (Protein Medley, Takara, Kusatsu, Japan, Cat. No. 635323) were diluted and prepared according to manufacturer’s instructions (protocol PT1602-1) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... A 2nd PCR was performed with high-fidelity PrimeSTAR GXL DNA Polymerase (Takara Cat: R050B) to add the Nextera indices (Nextera XT primers Set A 96 Indexes ...
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bioRxiv - Cell Biology 2023Quote: ... and the 5′-end was phosphorylated with T4 polynucleotide kinase (Cat. # 2021S: Takara, Kyoto, Japan). This phosphorylated DNA fragment was ligated to the Bbs I cloning site in pSpCas9(BB)-2A-Puro (PX459 ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were cultured using Cellartis DEF-CS 500 culture system (Takara Bio, cat. no. Y30012).
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bioRxiv - Genomics 2023Quote: ... purified RNA were constructed with the SMARTer Stranded RNA-seq Kit (Takara, cat. no. #634839) according to the manufacturer’s instructions for first- strand cDNA synthesis ...
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bioRxiv - Cancer Biology 2023Quote: ... Lenti-X GoStix Plus were then used to determine virus titer (Takara Bio cat#631280). For lentiviral infection ...
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bioRxiv - Cell Biology 2023Quote: ... Contaminant DNA in 50 µg of extracted RNA was digested with DNaseI (cat. #2270A, Takara) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... Each library was indexed using the DNA Unique Dual Index Kit (Takara Bio, Cat. # R400665) for multiplexed sequencing using the Illumina NextSeq550 platform at the Genome Sequencing Core at the University of Kansas ...
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bioRxiv - Microbiology 2023Quote: ... Synthesized DNA was cloned into the pDON-5 Neo-vector (TaKaRa, Kusatsu, Japan, Cat# 3657), which was prelinearized with NotI-HF (New England Biolabs [NEB] ...
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bioRxiv - Genomics 2024Quote: ... with three markers (λ-Hind III digest; Takara Cat. No. 3403, DL15,000 DNA Marker; Takara Cat ...
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bioRxiv - Microbiology 2024Quote: ... TaqMan-qPCR was performed using the Premix Ex Taq (Probe qPCR) Kit (TaKaRa, cat# RR390L) in an StepOne Plus Real-Time PCR system (Applied Biosystem ...
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bioRxiv - Cell Biology 2024Quote: ... Virus titer was measured by using Lenti-X p24 Rapid Titer Kit (Cat #022261, Takara).
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bioRxiv - Immunology 2021Quote: ... and the protein concentration was measured by BCA protein assay kit (TaKaRa, Dalian, China, cat#T9300A) as previous described(Ma et al. ...
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bioRxiv - Molecular Biology 2021Quote: RNAi plasmids were constructed using the In-Fusion® HD Cloning Kit (Takara Bio; Cat #: 639650) and the L4440 vector ...
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bioRxiv - Developmental Biology 2021Quote: ... at a density of 0.8× 104 cells per cm2 in NDiff227 media (Takara cat. no. Y40002) supplemented with 1% KSR (Gibco cat ...
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bioRxiv - Molecular Biology 2021Quote: ... qPCR was performed in duplicate samples using TB Green Premix Ex Taq II (Takara, cat# RR820S) and Thermal Cycler Dice (Takara ...
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bioRxiv - Genomics 2020Quote: ... mix was added (0.475μl SmartScribe [Takara], 0.125μl RNase inhibitor [Takara, cat no. 2313A], 1μl 5X First strand buffer [Takara] ...
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bioRxiv - Immunology 2021Quote: ... Real-time PCR reactions were performed using TB Green Premix Ex Taq reagent (TaKaRa, Cat#RR420A) on QuantStudio 7 Flex (Applied Biosystems) ...
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bioRxiv - Neuroscience 2019Quote: ... and human WT hP-NPO cDNA was amplified from human brain cDNA library (TaKaRa, Cat #637242) with primers ...
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bioRxiv - Biophysics 2021Quote: ... viral particles in the cell pellets were purified with AAVPro purification kit (Takara bio cat. 6675) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... and then concentrated using the lenti-x concentrator (Cat. No. 631231, Clontech Laboratories, Mountain View, CA).
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bioRxiv - Neuroscience 2020Quote: ... Sequencing libraries were prepared using SMARTer Stranded Total RNA-seq kit (Clontech Laboratories, Cat. No. 634876). All steps involved were performed according to manufacturer’s protocol.
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bioRxiv - Microbiology 2022Quote: ... GISAID ID: EPI_ISL_408667)29 were prepared by PCR using PrimeSTAR GXL DNA polymerase (Takara, Cat# R050A). A linker fragment encoding hepatitis delta virus ribozyme ...
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bioRxiv - Microbiology 2022Quote: ... We then assembled these fragments by CPER using PrimeSTAR GXL DNA polymerase (Takara Bio, Cat# R050A). The assembled cDNA was transfected into BHK-hACE2 cells using Lipofectamine LTX Reagent with PLUS™ Reagent (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: All expression plasmids are based on the lentiviral vector pLVX-EF1α-mCherry-N1 (Takara, Cat# 631986). Codon-optimized variants of GPR133 were previously published and described (Frenster et al. ...
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bioRxiv - Microbiology 2021Quote: ... GISAID ID: EPI_ISL_408667)37 were prepared by PCR using PrimeSTAR GXL DNA polymerase (Takara, cat# R050A). A linker fragment encoding hepatitis delta virus ribozyme ...
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bioRxiv - Cancer Biology 2021Quote: ... Each gDNA was amplified as a technical triplicate with unique indexes using ExTaq (Takara Cat#RR001A) with PCR program of 95°C for 5 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA synthesis was performed with SMART- Seq v4 Ultra Low Input RNA Kit (Clontech cat. 634888) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2019Quote: ... Reverse transcription was performed to generate cDNA by using PrimeScriptTM RT reagent Kit (Takara, cat. # RR047A). Quantitative PCR was performed on LC480 (Roche ...
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bioRxiv - Molecular Biology 2021Quote: ... The experiment was conducted following the manufacturer’s instructions (Cat. No. 630489; Clontech, Mountain View, CA, USA).
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bioRxiv - Physiology 2019Quote: Total RNA extraction was performed using NucleoSpin RNA XS kit (Clontech Laboratories Inc. Cat. No. 740902). Briefly ...
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bioRxiv - Genetics 2021Quote: ... three eyes (lens removed) from different zebrafish were homogenized in RNAiso Plus reagent (Takara, Cat# 9108). Total RNAs were extracted following the operation manual and stored at -70°C ...