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Citations for Takara Bio :
451 - 500 of 1268 citations for pVectOZ CAT Transfection control since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... suspensions of the transformants were spotted onto SD plates with dropout supplements: Leu/-Trp (cat #630417) or -Ade/-His/-Leu/-Trp (cat #630428) (Takara Bio). The strains used in this assay are listed in Table S1.
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bioRxiv - Systems Biology 2023Quote: ... Genomic DNA was extracted from cell pellets using the Machery Nagel NucleoSpin Blood Kit (Midi, Clontech Cat. #740954.20; XL Maxi, Clontech Cat. #740950). The detailed protocol is available at http://www.broadinstitute.org/rnai/public/resources/protocols.
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bioRxiv - Cancer Biology 2023Quote: ... In short: 3.5 cm non-coated cell culture dishes (Falcon, cat 351008) were coated using 12 µg Retronectin (Takara, cat T100B). Coated dishes then were pre-incubated with 1 ml viral supernatant for 4-5 hours at 37oC ...
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bioRxiv - Immunology 2020Quote: ... Cell supernatants were collected at 24 and 48 h after transfection of HEK 293/17 cells and concentrated using a LentiX concentrator (Takara Bio USA, Inc., Mountain View, CA). HEK 293/17 cells or NSC cells were plated in 6 well plates at a density of 105 per well ...
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bioRxiv - Cell Biology 2022Quote: ... while pGADT7-CmVPS41s and pGADT7-T control prey plasmids were transformed into yeast strain Y187 following the Yeastmaker Yeast Transformation System 2 instructions (Clontech by Takara Bio, Mountain View, USA). The transformed cells were grown either in SD-Trp agar plates for Y2HGold or in SD-Leu agar plates for Y187 at 30 ºC for 3-5 days ...
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bioRxiv - Genomics 2020Quote: ... corresponding primers (Supplementary Table 3-4), Phanta Max Super-fidelity DNA Polymerase (Cat. No. P505-d1, Vazyme) or KOD plus (Cat. No. F0934K, Takara, Kyoto, Japan) with a touchdown cycling protocol that contains 30 cycles of 98 °C for 10 s ...
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bioRxiv - Immunology 2021Quote: ... PrimeScript™ RT Reagent Kit with gDNA Eraser (Perfect Real Time, Cat. #RR047A) and TB Green® Premix Ex Taq ™ (Tli RNaseH Plus, Cat. # RR402A) were purchased from Takara Bio Inc ...
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bioRxiv - Microbiology 2020Quote: ... we PCR amplified HXB2 env from the pIIIenv3-1 plasmid (NIH AIDS Reagent Program cat. #289) and cloned it into the pLVX-EF1α-IRES-Puro lentiviral vector (Clontech cat. #631988). J-Lat clone 11.1 cells were transduced with lentiviral particles produced from this plasmid and stable cell lines were generated using puromycin selection.
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was removed using the DNA-free kit (Thermo Fischer Scientific, Cat# AMl906) and subsequent cDNA synthesis was performed using the PrimeScript RT reagent Kit (TAKARA, Cat# RR037A), both according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... then incubated with TALON magnetic beads (Clontech, cat# 35636) for 20 min at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit α-RFP (1:500; Clontech, cat# 632496, RRID:AB_10013483); mouse α-lacZ antibody (1:100 ...
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bioRxiv - Biophysics 2022Quote: ... A Puromycin resistance plasmid (pPUR, Clontech, Cat No. 631601) was used as a selection marker ...
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bioRxiv - Neuroscience 2022Quote: ... and 2 μg/ml Doxycycline (Clontech; Cat. No. 631311). iTF-iPSCs were counted and seeded onto double coated plates (Poly-D-Lysine-precoated Bio plates (Corning ...
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bioRxiv - Developmental Biology 2021Quote: ... and human-specific GFAP marker STEM123 (TaKaRa, cat. Y40420). As general astrocyte markers GFAP (DAKO ...
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-GFP (JL8 - Takara cat# 632381, RRID: AB_2313808), mouse anti-E-cadherin (HecD1-provided by M ...
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bioRxiv - Biochemistry 2020Quote: ... Takara Clontech pTRE3g bidirectional plasmid (Takara Clontech Cat# 631337) for inducible expression was subcloned from Addgene plasmids #89470 & #89471 ...
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bioRxiv - Biochemistry 2020Quote: ... Takara Clontech pTRE3g bidirectional plasmid (Takara Clontech Cat# 631337) for inducible expression was subcloned from Addgene plasmids #89470 & #89471 ...
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bioRxiv - Plant Biology 2020Quote: ... then incubated with TALON magnetic beads (Clontech, cat# 35636) for 20 min at 4 °C ...
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bioRxiv - Neuroscience 2021Quote: ... and 2 ug/mL doxycycline (Clontech, Cat. No. 631311). i3Neurons were then fed three times a week by half media changes ...
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bioRxiv - Molecular Biology 2020Quote: ... HRP-linked (1:2000, Takara, japan, Cat#T7122A-1);
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bioRxiv - Cell Biology 2021Quote: ... with RNase inhibitor (0.04U/uL final, Clontech Cat #2313A). Cultured cells were homogenized by trituration by pipet ...
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bioRxiv - Molecular Biology 2022Quote: ... and purified by MPG streptavidin beads (Takara, cat. 6124A) followed by RNAse (Promega ...
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bioRxiv - Microbiology 2022Quote: ... 0.8 U/μl recombinant RNase inhibitor (Takara, Cat# 2313B)] and incubated at room temperature for 10 min ...
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bioRxiv - Neuroscience 2022Quote: ... and fresh 0.2% RNase inhibitor (Takara Bio, Cat# 2313A)) for 15 minutes ...
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bioRxiv - Microbiology 2022Quote: ... and Ex Taq DNA Polymerase (TaKaRa Bio, Cat.:RR001), to add sequencing indexes to each amplicon ...
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bioRxiv - Microbiology 2022Quote: ... The 5 mL HisTALON cartridge (Clontech Laboratories, Cat# 635683) column was equilibrated with ten column volumes of Equilibration Buffer (HisTALON Buffer Set ...
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bioRxiv - Genomics 2022Quote: ... and treated with DNase I (Cat# 2270A, Takara Bio). cDNA was synthesized using SuperScript III First-Strand Synthesis SuperMix (Cat# 18080400 ...
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bioRxiv - Biophysics 2022Quote: ... A Puromycin resistance plasmid (pPUR, Clontech, Cat No. 631601) was used as a selection marker ...
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bioRxiv - Neuroscience 2021Quote: ... and combined with Lenti-X concentrator (Takara, cat# 631232). The mixture was incubated over night at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... 0.8 U/μl recombinant RNase inhibitor (Takara, cat# 2313B)] and incubated at room temperature for 10 min ...
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bioRxiv - Cancer Biology 2020Quote: ... using standard In-Fusion cloning (Takara Bio, Cat # 638911) to create pLEGB ...
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bioRxiv - Cancer Biology 2022Quote: ... concentrated using Lenti-X™ Concentrator (TaKaRa, Cat. #631232), and stored in aliquots at −80 °C ...
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bioRxiv - Synthetic Biology 2022Quote: ... coli cells: Stellar competent cells (Takara Bio, Cat#636763), chemically competent DH5α cells or NEB 10-beta competent cells (NEB ...
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bioRxiv - Bioengineering 2020Quote: ... and –His/–Trp/–Ura dropout supplement (Clontech, cat. 630424). Colonies were grown in SD/–His/–Trp/–Ura medium for one night at 30 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... HRP-linked (1:2000, Takara, Japan, Cat# T7122A-1).
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bioRxiv - Microbiology 2021Quote: ... 0.8 U/μl recombinant RNase inhibitor (Takara, Cat# 2313B)] and incubated at room temperature for 10 min ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was extracted with TRIzol (Takara, Cat. #9108) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... pAcGFP-Golgi was bought from Clontech (Cat. No.632464). Halo-Rab6 was generated by inserting Rab6A coding sequence obtained by PCR using HeLa cDNA as template into Halo-C1 vector ...
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bioRxiv - Microbiology 2021Quote: ... The 5 mL HisTALON cartridge (Clontech Laboratories, Cat # 635683) column was equilibrated with 10 column volumes of Equilibration Buffer (HisTALON™ Buffer Set ...
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bioRxiv - Immunology 2021Quote: ... and 3 U Recombinant RNase Inhibitor (Takara, Cat#2313A), sealed and immediately frozen on dry ice ...
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bioRxiv - Immunology 2021Quote: ... or KAPA Taq PCR Kit (Takara Bio, cat. R040A). Primers Rosa26 WT F ...
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Activity-regulated growth of motoneurons at the neuromuscular junction is mediated by NADPH oxidasesbioRxiv - Neuroscience 2022Quote: ... Rabbit-anti-dsRed (1:1000) (ClonTech Cat. No. 632496), Mouse nc82 (Bruchpilot ...
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bioRxiv - Cell Biology 2022Quote: ... mouse α-GFP JL-8 (cat. no. 632381, Takara) used at a concentration of 1:2,000 ...
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bioRxiv - Genomics 2022Quote: ... small intestine (cat. no. 636539, Takara Bio USA, Inc), and mammary gland (cat ...
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bioRxiv - Microbiology 2022Quote: ... and PrimeSTAR® GXL DNA polymerase (TaKaRa, cat R050B) were used for cDNA preparation ...
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bioRxiv - Microbiology 2022Quote: ... SMARTer® PCR cDNA synthesis kit (TaKaRa,cat 634926) and PrimeSTAR® GXL DNA polymerase (TaKaRa ...
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bioRxiv - Neuroscience 2023Quote: ... and 2 μg/ml doxycycline (Clontech, cat. no. 631311). Neuronal induction media was changed once a day for 2 more days ...
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bioRxiv - Genetics 2022Quote: ... 1.5μL Titanium Taq DNA Polymerase (Clontech Takara, Cat# 639242), 8 μL dNTPs (Clontech Takara Cat# 4030) ...
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bioRxiv - Genetics 2022Quote: ... 1.5μL Titanium Taq DNA Polymerase (Clontech Takara, Cat# 639242), 8 μL dNTPs (Clontech Takara Cat# 4030) ...
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bioRxiv - Neuroscience 2022Quote: ... dsRED (rabbit polyclonal, Living Colors, Clontech, Cat no. 632496); MAP2 (mouse monoclonal ...