Labshake search
Citations for Takara Bio :
5301 - 5321 of 5321 citations for Human Lysine K Specific Demethylase 1A KDM1A ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: A codon-optimized synthetic gene corresponding to the full length nucleocapsid protein (Thermo GeneArt, Regensburg, Germany) was cloned into a modified pET28a vector using the In-Fusion cloning kit (Takara Bio Saint-Germain-en-Laye, France). The resulting plasmid encoded for an N-terminal His6 tag followed by thrombin and tobacco etch virus cleavage sequences upstream of the full-length nucleocapsid protein sequence ...
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bioRxiv - Plant Biology 2021Quote: ... and 500 ng total RNA was incorporated for first-strand cDNA synthesis using the M-MLV reverse transcriptase kit (TaKaRa Bio USA, Mountain View, CA, USA), and the library was used as a template for qRT-PCR analysis ...
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bioRxiv - Cell Biology 2020Quote: ... A total of 600 pg of RNA was used as input for the synthesis of cDNA with the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Bio USA, Mountain View, CA, USA). Manufacturer suggested protocol was followed ...
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bioRxiv - Biochemistry 2019Quote: ... cochleariae fat body for 3’ rapid amplification of cDNA ends (3’ RACE) was synthesized according to the manual from SMARTer RACE 5’/3’ Kit (Takara Bio, Inc. Mountain View, CA, USA). All cDNA templates were stored at −20 °C after synthesis.
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bioRxiv - Microbiology 2020Quote: The open reading frame of hetL gene mutated to encode for a D151A substitution was amplified using the hetL pRL fw and hetL pRL rv infusion primers with pKT25-hetLD151A as template and cloned into the BamHI restriction site of the pRL25T-PpetE replicative plasmid in Nostoc (Takara In-Fusion® HD Cloning kit).
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bioRxiv - Microbiology 2023Quote: ... The sequencing library was prepared using the SMART-Seq v4 Ultra Low Input RNA Kit with nonstranded and polyA+ selection (Clontech, Takara Bio USA, Mountain View, CA, USA). Approximately 120 million 125-bp paired-end reads per sample were obtained (HiSeq 2500 ...
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bioRxiv - Immunology 2022Quote: ... an amount of 1 ng of total RNA was used as input for the SMART-Seq® Stranded Kit (Cat. No. 634444; protocol version “022829”; low input option, Takara Bio USA, San Jose, USA). This kit can deal with degraded as well as high-integrity input RNA ...
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bioRxiv - Immunology 2023Quote: ... Library construction was performed based on manufacturer’s recommendation for the SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio USA Inc., California, USA) followed by the Nextera® XT DNA Library Prep Kit (Illumina ...
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bioRxiv - Evolutionary Biology 2023Quote: We prepared each sequencing library from 1 μg of total RNA using a PrepX PolyA mRNA Isolation Kit (Wafergen, 40047) on the Apollo 324 NGS Library Prep System (Takara Bio USA Holdings, Mountain View, CA). We added Illumina indices and amplified libraries with 12 cycles of PCR and cleaned them using PCRClean DX beads (Aline Biosciences ...
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bioRxiv - Biophysics 2023Quote: Plasmids used in this study (Supplementary Table S1) were assembled by Polymerase Chain Reactions (PCR) amplifying insert and vector DNA fragments followed by In-Fusion cloning (Takara Biosciences, In-Fusion HD Cloning Kit). Oligonucleotides (Integrated DNA Technologies ...
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bioRxiv - Plant Biology 2023Quote: ... and cloned into the pSL vector containing the Nicotiana tabacum PR1 signal peptide using the In-fusion cloning kit (Takara Bio USA Inc., San Jose, USA) to allow efficient secretion in N ...
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bioRxiv - Microbiology 2022Quote: ... Samples showing successful RNA extraction after checking by Bioanalyzer electrophoresis were reverse transcribed and cDNA amplified using the SmartSeq® v4 Ultra® Low Input RNA Kit for Sequencing (Takara Bio. Inc., Shiga Prefecture, Japan). Successfully amplified samples were prepared for paired end short-read sequencing using the Nextera XT Library Prep kit (Illumina ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR1 (10 cycles of amplification) was performed with the addition of SMARTer RNA Unique Dual Index Set A and Set B (Takara SMARTer RNA Unique Dual Index Kit-96A). Samples were pooled into quadrants and depletion of ribosomal cDNA was performed ...
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bioRxiv - Zoology 2019Quote: Amplification of 5ʹ and 3ʹ ends of cDNA was performed according to the protocol described in the SMART RACE cDNA Amplification Kit (Takara Bio USA, Inc. Mountain View, CA, USA). Three pairs of gene-specific primers (GSP) ...
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bioRxiv - Genomics 2022Quote: ... 0.5 μg of total RNA from each sample was used to synthesize cDNAs from polyA+ RNA using Clontech SMART-Seq® v4 Ultra® Low Input RNA Kit for the Fluidigm® C1™ System (Clontech, Cat. No. 635026) following the manufacturer’s guidelines ...
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bioRxiv - Genomics 2022Quote: ... cDNAs from low yield polyA+ RNA was synthesized using SMART-Seq® v4 Ultra® Low Input RNA Kit for the Fluidigm® C1™ System (Clontech, Cat. No. 635026), SMARTer-seq® chemistry (Switching Mechanism at 5’ End of RNA Template and Locked Nucleic acid technology) ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR was introduced to measure the relative expression of RDase genes by using the One Step PrimeScript™ RT-PCR Kit (Perfect Real Time) (Takara Bio, Saint-Germain-en-Laye, Germany). Primers targeting RDase genes were designed using the NCBI primer design tool with the setting parameters ...
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bioRxiv - Neuroscience 2023Quote: ... We obtained 20 ng/ul RNAs (total 10 ul eluted RNA from one microdissected cell body) that we used for RNAseq (Clontech SMART-Seq Ultra Low Input RNA kit) in Scripps Florida Genomics Core (Currently known as The Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology) ...
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bioRxiv - Microbiology 2024Quote: ... encoding region at the 3’ terminus of a putative linearized ambi-like virus was done following the protocol of the SMARTer® RACE 5’/3’ Kit (Takara Bio USA, Mountain View, CA, USA), employing a specific primer designed by Primer3-2.3.7 under Geneious (Supplemental Table S2) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Library purification was achieved by pooling and purifying labelled amplicons using the NucleoSpin® Gel and PCR Clean-up kits (Takara Bio USA, Inc, Mountain View, CA USA) with three modifications to the NucleoSpin® User Manual ...
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bioRxiv - Evolutionary Biology 2024Quote: Whole transcriptome amplification for three Spirostomum populations from the Katz lab was performed using the SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (TaKaRa Bio USA, Inc., Mountain View, CA, USA). Amplified cDNA was purified using the AMPure XP PCR Purification system (Beckman Coulter Life Sciences ...