Labshake search
Citations for Takara Bio :
5251 - 5300 of 5321 citations for Human Lysine K Specific Demethylase 1A KDM1A ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2023Quote: ... RNA input was normalized across samples and RNA libraries were prepared utilized the SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (Takara, 634890, 634888) followed by the Nextera XT DNA library preparation kit (Illumina ...
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bioRxiv - Evolutionary Biology 2024Quote: ... WTAs of the individual ciliates were generated according to the Smart-seq2 protocol (Picelli et al. 2014) and SMART-Seq v4 Ultra Low Input RNA Kit (TaKaRa Bio-medicals, Seoul, Korea) using 17 cycles of cDNA amplification for each cell ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The rRNA genes (18S, ITS, 28S) were amplified by polymerase chain reaction (PCR) using the TaKaRa Ex Taq polymerase kit (TaKaRa Bio-medicals, Seoul, Korea). PCR cycling parameters and primer information were followed as in Shazib et al ...
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bioRxiv - Cell Biology 2024Quote: ... The PCR product was cloned into the KpnI site of the pCRtk2×2NN vector (RDB18670) to generate pCRtk-PDX1 using an In-Fusion HD Cloning Kit (639648; Takara Bio Inc., Shiga, Japan). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA from the same amount of RNA between RNAi and control samples were synthesized in a 20 μl reaction volume by the PrimeScript™ RT reagent Kit with gDNA Eraser (TAKARA, Code No. RR047A). One μl of cDNA was used as the template in a 10 μl reaction volume of the PowerUp SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Neuroscience 2024Quote: ... Ultra-low input RNA sequencing library was prepared by using SMART-Seq HT kit for full-length cDNA synthesis and amplification (Takara, San Jose, CA, USA), and Illumina Nextera XT (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... The amplified linear fragment of pKNOCK-Km and the internal fragments of each gene were fused using In-Fusion® HD Cloning Kit (Takara Bio, Shiga, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... 0.5 µg of the isolated RNA was reverse transcribed to cDNA using an oligo dT primer and 12.5 units of reverse transcriptase mix (PrimeScript RT Reagent Kit, TaKaRa Bio, Saint-Germain-en-Laye, France). cDNA corresponding to 10 ng total RNA served as the template in a real-time PCR ...
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bioRxiv - Molecular Biology 2020Quote: ... was amplified by RT-PCR and cloned into pMSCV-IRES-eGFP using In-Fusion® HD Cloning Kit User Manual (Takara, Cat#638920, Shiga, Japan) following the manufacturer’s instructions (see Table 3 for primer sequences).
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bioRxiv - Immunology 2019Quote: ... Library construction was performed with the “SMARTer® Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian” (TaKaRa Bio USA, Inc., Mountain View, CA, USA) with a final multiplexing of 11 libraries according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2019Quote: ... to perform 5′ and 3′ rapid amplification of cDNA ends (RACE)-PCR utilizing the Clontech SMARTer 5′/3′ RACE Kit (Takara BIO USA Inc, CA, USA) as recently described58 ...
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bioRxiv - Microbiology 2020Quote: ... was reverse-transcribed to cDNA using random hexamers and oligo (dT) by following the instructions provided in the Prime-Script RT Reagent Kit (Perfect Real Time from TaKaRa Bio Inc., Otsu Shiga, Japan). RT-qPCR was carried out in a StepOnePlus Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Systems Biology 2020Quote: ... RNA was eluted in 10 µl water and immediately proceeded to cDNA generation using the SMART-Seq V4 Ultra Low RNA Kit (Takara Bio USA, Mountain View, CA). 1 ng of the resulting cDNA from 14 PCR cycles was “tagmented” and barcoded by using a Nextera XT DNA Sample Preparation Kit (Illumina) ...
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bioRxiv - Microbiology 2021Quote: ... recombinant sites including the 900 base pair upstream/downstream flanking region and the 100 base pair coding sequence of the target genes were cloned into pK18-CmR-pheS** plasmid backbone by using an In-Fusion® HD Cloning Kit (TAKARA Bio; Kusatsu, Shiga, Japan) to generate the plasmid (aedF- ...
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bioRxiv - Zoology 2021Quote: ... The reverse transcription of 1 μg of total RNA was performed using PrimeScript® RT reagent kit with gDNA Eraser (RR047A, Takara Bio Inc., Dalian, China). The gene expression was determined through RT-PCR ...
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bioRxiv - Biochemistry 2022Quote: ... one microgram of total RNA was employed to obtain the corresponding cDNA target sequences using an oligo(dT)18 primer and the PrimeScript RT reagent kit (Perfect Real Time, Takara Bio Inc., Otsu, Shiga, Japan), following the manufacturer’s directions ...
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bioRxiv - Microbiology 2021Quote: Antibody sequencing was performed as previously described (Simonich et al., 2019; Vigdorovich et al., 2016) using the SMARTer RACE 5’/3’ Kit (Takara Bio USA Inc., Mountainview, CA). Briefly ...
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bioRxiv - Evolutionary Biology 2022Quote: ... library was prepared according to the Isoform Sequencing protocol (Iso-Seq) using the Clontech SMARTer® PCR cDNA Synthesis Kit (Clontech Laboratories (now Takara Laboratories), 634926 ...
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bioRxiv - Microbiology 2019Quote: ... 300 ng of each RNA was reverse transcribed to cDNA using random hexamers and oligo(dT) primers and following the instructions provided in the PrimeScript RT Reagent Kit (Perfect Real Time from Takara Bio Inc., Otsu Shiga, Japan). RT-qPCR was carried out in a StepOnePlus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Neuroscience 2021Quote: ... Flash frozen cell samples were sent to the Cambridge Genomic Services for library preparation using SMART-Seq® v4 Ultra® Low Input RNA Kit (Takara Bio USA, Inc), followed by RNA sequencing (NextSeq 74 cycle high output 15-20 M reads per sample ...
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bioRxiv - Physiology 2020Quote: ... The PCR products were subsequently cloned into an EcoRI-HF® (New England BioLab, MA, USA) linearized pIRESZ2_ZsGreen1 vector using In-Fusion® HD cloning kit (TaKaRa Bio Inc, Kusatsu, JP). After sequence validation (Eurofin ...
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bioRxiv - Genetics 2020Quote: ... nucleotides 610–792 of the map3 ORF were subjected to random mutagenesis by using the Diversify PCR Random Mutagenesis Kit (Clontech Laboratories, Mountain View, CA, USA) in accordance with the manufacturer’s instructions for error-prone PCR ...
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bioRxiv - Plant Biology 2021Quote: ... and the library preparation was made with the SMART-Seq™ v4 Ultra™ Low Input RNA Kit for Sequencing (Takara, Mountain View, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... The cDNAs that were used for RACE were synthesised from 1 µg of polyA+ RNA using a SMART RACE cDNA Amplification Kit (Clontech Laboratories, Inc., Palo Alto, CA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... cDNA was synthesized from 1 ng RNA using the SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (Takara Bio Europe SAS). Libraries were prepared from the purified cDNA using the Nextera™ XT DNA Library Preparation Kit (Illumina) ...
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bioRxiv - Genomics 2019Quote: The 90-μL preparation volume for PCR contains 1X Advantage 2 PCR Buffer [not short amplicon (SA)](10X, Clontech, 639206, Advantage® 2 PCR Kit), 0.4-mM dNTP Mix (50X/10 mM ...
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bioRxiv - Microbiology 2022Quote: ... The amplified 0.5 kbp fragment was inserted into the BamHI site of pTK2051 using the In-Fusion Snap Assembly kit (Takara Bio USA, Mountain View, CA, USA). The resulting suicide plasmid was introduced into B ...
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bioRxiv - Systems Biology 2022Quote: ... The PCR products were subsequently cloned into pGEMHE Xenopus laevis oocyte expression vector using In-Fusion® HD cloning kit (TaKaRa Bio Inc, Kusatsu, JP) and the final sequence validated (Eurofin ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were amplified and synthesized into cDNA according to the manufacturer’s protocol using the SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (Takara Bio Inc., Shiga, Japan) and amplification yield was checked using a Bioanalyzer (Agilent High Sensitivity DNA Kit ...
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bioRxiv - Microbiology 2022Quote: ... The amplified fragments were then fused with SmaI-linearized pUt-C-EGFP10 using the In-Fusion HD Cloning Kit (Clontech Laboratories, Mountain View, CA, USA). Gene sequences fused with the egfp gene were positioned in tandem between the linker sequences ...
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bioRxiv - Microbiology 2022Quote: ... DNAse treated-RNA was reverse transcribed into cDNA using random hexamers and oligo (dT) primers with the Prime-Script RT Reagent Kit (Perfect Real Time from Takara Bio Inc., Otsu Shiga, Japan). Viral presence was assessed through RT-qPCR (StepOnePlus Real-Time ...
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bioRxiv - Cancer Biology 2024Quote: We applied 5’- and 3’-RACE assays to amplify the full length of tsTE1 transcript from the cRNA of MHCC-97L cells using the SMARTer™ RACE cDNA Amplification Kit (Clontech, Mountain View, CA, USA) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... The full-length CD177 cDNA inserts were sub-cloned into pBMN-I-EGFP retroviral vector digested with Bam HI and EcoR I restriction enzymes using InFusion HD Cloning Kit (Takara Bio USA, Mountain View, Ca) according to the product manual ...
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bioRxiv - Bioengineering 2023Quote: ... Purification and titer determination of the adenoviral particles were carried out using the Adeno-X™ Maxi Purification Kit (Takara Bio USA, San Jose, CA) and QuickTiter™ Adenovirus Titer ELISA Kit (Cell Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... The amplified fragments were then fused with SmaI-linearized pisCIIA-mCherry (Mamun et al, 2020) using the In-Fusion HD Cloning Kit (Clontech Laboratories, Mountain View, CA, USA). Recombinant plasmids for the expression of mCherry-fused 19 proteins were digested with NotI and ectopically inserted into the ATP sulfurylase (sC ...
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bioRxiv - Immunology 2023Quote: ... the library was prepared by synthesizing cDNA via reverse transcription and amplifying cDNA by long-distance PCR using the Takara SMARTer v4 kit (Takara Bio USA, San Jose, CA). 2×150bp sequencing was performed on an Illumina NovaSeq6000 instrument ...
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bioRxiv - Developmental Biology 2023Quote: ... IVF embryos were lysed in lysis buffer as described and cDNA was prepared using SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing (Takara Bio, catalong #634890). cDNA was tagmented using Nextera XT DNA Library Preparation Kit (Illumina catalog #FC-131-1024 ...
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bioRxiv - Molecular Biology 2023Quote: ... were constructed by cloning the full-length coding region into the pGADT7 vector using the In-Fusion HD Cloning Kit (Clontech Laboratories, Mountain View, CA, USA). The Matchmaker two-hybrid system 3 (Clontech Laboratories ...
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bioRxiv - Molecular Biology 2023Quote: ... Amplicons were sized in gels (as above) and bands of interest isolated using the NucleoSpin Gel and PCR Clean-up kit (Takara Bio Inc., San Jose, CA). Sanger Direct Sequencing was performed at Azenta Life Sciences (South Plainfield ...
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bioRxiv - Plant Biology 2024Quote: ... and a 500-ng RNA was used for the first-strand cDNA synthesis using PrimeScript™ 1st strand cDNA Synthesis Kit (6110A; TaKaRa, San Jose, California, USA) with oligo(dT ...
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bioRxiv - Physiology 2024Quote: Total RNA (1 µg) was used as input for SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Cat. # 634875; Takara, Saint-Germain-en-Laye, France). Sequencing was performed on the NextSeq 2000 instrument (Illumina ...
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bioRxiv - Neuroscience 2024Quote: ... The PCR-amplified promoter fragments were inserted into the XhoI-AgeI site of the pAAV using Ligation high Ver.2 (LGK-201; Toyobo: hGFAP(ABC1D) or In-Fusion HD Cloning Kit (Takara Bio, Shiga, Japan: mMBP promoter).
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bioRxiv - Molecular Biology 2024Quote: ... 10 ng of RNA was used as input for cDNA synthesis using the SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Takara Bio, San Jose, CA, USA). Library preparation was performed using the Nextera XT DNA Library Preparation Kit (Illumina) ...
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bioRxiv - Physiology 2024Quote: Libraries were prepared by the Van Andel Genomics Core (Grand Rapids, MI) from 1 ng of total RNA using Takara SMART-Seq Stranded Kit (Takara Bio USA, Mountain View, CA) per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... was amplified using the hetL pET28 fw and hetL pET28 rv infusion primers and cloned into the XhoI and NcoI restriction sites of the pET28a expression plasmid using the In-Fusion technology (Takara In-Fusion® HD Cloning kit).
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bioRxiv - Microbiology 2020Quote: ... was amplified using the patS pRL fw and patS pRL rv infusion primers and cloned into the BamHI restriction site of the pRL1272-PpetE replicative plasmid in Nostoc (Takara In-Fusion® HD Cloning kit).
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bioRxiv - Microbiology 2020Quote: ... was amplified using the hetL pRL fw and hetL pRL rv infusion primers and cloned into the BamHI restriction site of the pRL25T-PpetE replicative plasmid in Nostoc (Takara In-Fusion® HD Cloning kit).
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bioRxiv - Developmental Biology 2020Quote: ... were transferred into 0.2-mL PCR tubes and processed for sequencing using Seq® v4 Ultra® Low Input RNA Kit (Takara Bio USA, Mountain View, CA) as described in Wood et al ...
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bioRxiv - Molecular Biology 2021Quote: ... the puromycin-selected transfectants were directly subjected to RT-qPCR analysis using the CellAmp™ Direct TB Green® RT-qPCR Kit (TaKaRa Bio Inc., Shiga, Japan) at 3 days after transfection ...
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bioRxiv - Cancer Biology 2019Quote: ... and 19 and BIRC2 was amplified using specific primers (Supplementary File 1) and cloned downstream of luciferase gene into pcDNA6/Luc(30) using an In-Fusion HD cloning kit (Takara Bio USA, Inc., Mountain View, CA). The recombinant pcDNA6/LUC-DUSP (or pcDNA6/LUC-DUSP-211Δ) ...