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Citations for Lonza :
4101 - 4130 of 4130 citations for Cytomegalovirus Cell Lysate Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... 1.3×10^5 C25cl48 cells/reaction were electroporated with a ribonucleoprotein consisting of 125 pmol TrueCut Cas9 Protein v2 (A36498) + 150 pmol gRNA + 120 pmol ssDNA using P5 Primary Cell 4D-Nucleofector X Kit S (Lonza, Walkersville, MD, V4XP-5032) in a 16-well Nucleocuvette Strip and the Amaxa 4D-Nucleofector X Unit (Lonza ...
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bioRxiv - Microbiology 2021Quote: African green monkey kidney epithelial Vero cells (ATCC® CCL-81) and Vero E6 cells (ATCC® CRL-1586) were maintained in Dulbecco’s modified Eagle’s medium (12-604F, Lonza, BioWhittaker®, Walkersville, MD, USA) supplemented with 10% Fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2021Quote: ... Plasmids (10 μg) were then linearized with NotI and transfected into SmOx P9 TbGPI2-KO cells using a 4D nucleofector system (Lonza Group AG, Basel, Switzerland) with program FI-115 ...
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bioRxiv - Cancer Biology 2019Quote: ... and 3-4 hours later were transfected with 2 μg of pBabe KRAS WT and pBabe KRAS G12D or pBabe KRAS Q61H plasmid DNA by electroporation using the Amaxa® Human T Cell Nucleofector® Kit (Lonza, Basel, Switzerland) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: ... mice were reconstituted 4 hrs post-irradiation with 5 million donor bone marrow cells in 200 μl Hank’s Balanced Salt Solution (HBSS; Lonza, distributed by VWR, Lutterworth, UK), administered by intravenous injection into the tail vein ...
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bioRxiv - Genetics 2020Quote: ... Reporter cell lines were generated by TALEN-mediated homology-directed repair to integrate a donor construct into the AAVS1 locus as follows: 1.2×106 K562 cells were electroporated in Amaxa solution (Lonza Nucleofector 2b, setting T0-16) with 1000 ng of reporter donor plasmid (Table S2) ...
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bioRxiv - Genetics 2021Quote: Human embryonic microglia clone 3 (HMC3, ATCC® CRL3304™) cells were cultured in minimum essential medium (MEM) Eagle-EBSS with NEAA without L-Glutamine (Lonza, Cologne, Germany) supplemented with 10% fetal bovine serum (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: All proteins were expressed using the baculoviral expression system and the lepidopteran High Five cell line in Insect-XPRESS (Lonza, cat. no. BELN12-730Q). Baculoviruses were produced using homologous recombination in insect cells by co-transfection with linearized baculoviral DNA and the transfer vector pAcGP67A (BD Biosciences) ...
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bioRxiv - Developmental Biology 2023Quote: ... mESCs were transfected with the pre-assembled fRNP pool by electroporation (program: OP315 or CD112) using an SE Cell line 4D-Nucleofector™ X kit (Lonza, Catalog#: V4XC-1024). The electroporated cells were plated in Nunc Glass Bottom Dishes (Thermo Fisher ...
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bioRxiv - Immunology 2023Quote: ... 2 million of CAR-T cells were electroporated with 1 μg Cas9 mRNA in 16-well Nucleocuvette™ Strips using EO-115 program (Lonza, per manufacturer’s instructions). Cells were expanded in human T cell media for another 3 days before analysis or transfer into mice.
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bioRxiv - Immunology 2023Quote: ... 2 million of CAR-T cells were electroporated with 1 μg Cas9 mRNA in 16-well Nucleocuvette™ Strips using EO-115 program (Lonza, per manufacturer’s instructions). Cells were expanded for another 2 days before transfer into NSG mice.
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bioRxiv - Cell Biology 2023Quote: ... to aid complete removal of PBS before resuspension in P3 Nucleofection Solution (room temperature; P3 Primary Cell 4D-NucleofectorTM X Kit S; Lonza; cat. no., V4XP-3032). A total of 20 μl solution was used to resuspend 80,000 cells ...
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bioRxiv - Cancer Biology 2023Quote: NPE cells were transfected by electroporation using a Lonza® Nucleofector 2b device and the Lonza® Mouse Neural Stem Cell Nucleofector Kit (Lonza; #VPG-1004) according to the manufacturer’s instructions using the T-030 pulse code.
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bioRxiv - Microbiology 2023Quote: Embryonic monkey kidney cells (MA104) were grown in Dulbecco’s modified Eagle’s medium (DMEM) containing 4.5 g/L glucose (Lonza 12-640F or Corning 15-107-CV), 1% penicillin-streptomycin [Corning]) ...
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bioRxiv - Cell Biology 2022Quote: ... Cusps were placed in a petri dish filled with VEC medium (EGM™-2 MV Microvascular Endothelial Cell Growth Medium-2 BulletKit™ (Lonza, #C-3202)) and VECs were scraped into the medium using a sterile scalpel ...
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bioRxiv - Cell Biology 2022Quote: ... was induced in hVECs and pVECs on a 12-well plate using EGM™-2 MV Microvascular Endothelial Cell Growth Medium-2 BulletKit™ (Lonza, #CC-3202), without VEGF ...
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bioRxiv - Cancer Biology 2023Quote: ... 4T1 (ATCC #CRL-2539) and E0771 (CH3 Biosystems #940001) cell lines were culture in RPMI 1640 with L-Glutamine (Lonza™ BioWhittaker™ # BW12115F12) supplemented with 10% of FBS ...
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bioRxiv - Cancer Biology 2023Quote: CD4 SMARTA T cells were isolated from naïve mice and were washed and resuspended in 20 μl buffer P3 (P3 Primary Cell 4D-Nucleofector X Kit S, Lonza, cat. no. V4XP-3032). Between 105 to 107 cells were used per nucleofection ...
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bioRxiv - Bioengineering 2023Quote: ... Complete growth medium for culturing of human adipose derived stem cells (ASCs, ATCC SCRC-4000) consists of Lonza ADSC Basal Medium (Lonza, Lexington, MA PT-3273), 10% MSC FBS (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... For transient transfection 1e6 Jurkat cells were transfected with 2 μg DNA using the LONZA electroporator (program X-001) and Nucleofector Kit V (Lonza, catalog no. VCA-1003) 24 h prior to imaging ...
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bioRxiv - Synthetic Biology 2024Quote: ... The cell/DNA suspension was transferred into the certified Nucleofector® cuvette and the V-001 program of Nucleofector® 2b Device (Lonza, AAB-1001) was applied ...
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bioRxiv - Molecular Biology 2024Quote: ... counted and 1×106 cells electroporated using the CB-150 program of the 4D-Nucleofector™ System and the P3 Primary Cell 4D-Nucleofector® X Kit L (Lonza, #V4XP-3024) according the manufactures protocol and plated in E8+RI ...
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bioRxiv - Molecular Biology 2019Quote: ... 150,000 cells were resuspended in 20 µL of P3 Primary Cell Nucleofector Solution with Supplement 1 and mixed with the gRNA/rCas9 solutions before electroporation (4D-Nucleofector System, Lonza, Walkersville, MD; program DC-100). For experiments involving the Neon Transfection System (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2021Quote: Primary normal human bronchial epithelial cells (NHBECs) were obtained from a 65 year old Caucasian male without identifiers sourced from Lonza (NHBE CC-2540; Walkersville, MD). The NHBECs were revived from cryopreservation and expanded with PneumaCult™ Ex-Plus media (StemCell #05040 ...
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bioRxiv - Genomics 2022Quote: ... 1.0µg/µl SpCas9-sgRNA-PGK-Venus construct and 1µg/µl donor construct were nucleofected into ∼3×106 Tir1 mESCs using the Amaxa™ 4D-Nucleofector and the P3 Primary Cell 4D-Nucleofector™ X Kit (Lonza, Cat. V4XP-3024) following the manufacture’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... hTERT-HME1 (CRL-4010) were acquired from ATCC and cultured in Mammary Epithelial Cell Growth Medium Bullet Kit from Lonza (Bend, OR, Catalog #: CC-3150).
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bioRxiv - Immunology 2024Quote: 1-1.5 x106 triple reporter TCR-/- Jurkat cells were mixed with 2μg of pLVM containing gene of interest and electroporated using pulse code CL-120 according to manufacturer’s protocol (Lonza #V4XC-1024, SE Cell line kit). After 48-72hrs ...
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bioRxiv - Cell Biology 2023Quote: ... BU3 NG iPSCs were pretreated with ROCK Inhibitor (Y-27632) for three hours prior to nucleofection using the Lonza P3 Primary Cell 4DNucleofector™ X Kit (Lonza, cat. no. V4XP-3024) and replated for puromycin resistance screening ...
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bioRxiv - Neuroscience 2023Quote: ... were added to 20 µL P3 buffer containing 1.6 × 105 Accutase-dissociated iPSCs and the nucleofection procedure was carried out in a 16-well Amaxa 4D cuvette (Lonza; Primary Cell P3, pulse code CA137). Cells were cultured for 3 days under cold-shock conditions (32 °C/5% CO2 ...
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bioRxiv - Cancer Biology 2022Quote: ... PBMCs were isolated from peripheral blood from donors by Ficoll separation and cultured into X-Vivo 15 + 5% CTS Immune Cell SR (Lonza Cat# BE08-879H and Gibco Cat# A2596101) and activated with soluble anti-CD3 antibody (OKT3 ...