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Citations for Lonza :
4101 - 4150 of 4418 citations for Rat Neural Stem Cell Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 1 μl of isolated schizonts was mixed with 7 μl of DNA and 18 μl of P3 Primary Cell 4D-Nucleofector solution from Lonza. This mixture was then added to a well within a 4D-Nucleofector 16-well strip from Lonza and electroporated using the FI-115 program on the Amaxa Nucleofector 4D ...
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bioRxiv - Molecular Biology 2024Quote: ... 2×105 cells were electroporated with 250 ng of gRNA plasmids and 750 ng of CBE using the SF Cell Line Nucleofector X Kit (Lonza) via the 4D-Nucleofector system ...
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bioRxiv - Immunology 2024Quote: ... They were then transfected by nucleofection with 3 μg of plasmid DNA in 100 μl of Cell Line Nucleofector Solution V (Lonza) using the V-001 program (Amaxa Biosystems) ...
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bioRxiv - Developmental Biology 2024Quote: ... Sorted cells expressing medium levels of fluorescence were expanded and frozen then representative lots of each cell line were thawed and tested for mycoplasma contamination using the MycoAlert assay (Lonza), and fungi and aerobic bacteria sterility by inoculating conditioned medium into trypticase soya broth (Sigma Aldrich) ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were thawed according to the supplier’s instructions and expanded in collagen-coated flasks in human stellate cell growth medium (Lonza, MCST250) supplemented with 50 μM 2-Phospho-L-ascorbic acid (Merck ...
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bioRxiv - Cell Biology 2024Quote: ... with a density of 1.5 x106 using a 1:70 inoculation from the V1 stock for 92 hours at 21°C in Insect Xpress Protein-free Insect Cell Medium (Lonza) supplemented with GlutaMAX (GIBCO ...
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bioRxiv - Cancer Biology 2024Quote: ... All cell lines were routinely tested for Mycoplasma using a MycoAlert™ PLUS Mycoplasma Detection Kit (Cat# LT07-703, Lonza).
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bioRxiv - Cell Biology 2024Quote: ... The cell lines used in this study were routinely tested for mycoplasma contamination using MycoAlert Mycoplasma Detection Kit (#LT07-318, Lonza), and mycoplasma-negative cells were used ...
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bioRxiv - Immunology 2024Quote: ... 3 x 106 freshly isolated monocytes or BMDMs were resuspended in 20 µl of P3 primary cell nucleofection buffer (Lonza). Cells were then added to the Cas9-RNP complexes ...
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bioRxiv - Genomics 2024Quote: A Cas9/sgRNA RNP complex and linear dsDNA donor were transfected into HCT116 cells using the Amaxa Nucleofector II device (Lonza) set for program D-032 with the Cell Line Nucleofector Kit V ...
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bioRxiv - Synthetic Biology 2024Quote: ... Nucleofector program 96-FF-120 was used to electroporate K562 cells with the pDNA mix on the Amaxa Nucleofector 96-well Shuttle System (Lonza). After electroporation ...
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bioRxiv - Synthetic Biology 2024Quote: ... Nucleofector program 96-FF-120 was used to electroporate K562 cells with the pDNA mix on the Amaxa Nucleofector 96-well Shuttle System (Lonza). After electroporation ...
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bioRxiv - Cancer Biology 2024Quote: ... were annealed to Cas9 protein (IDT) and the ribonucleoprotein complex was transfected into SKOV-3 cells using a 4D-Nucleofector (Lonza). Bulk transfected cells were checked by flow cytometry for successful knock-down ...
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bioRxiv - Cancer Biology 2021Quote: ... Mycoplasma detection was routinely performed to ensure cells were not infected with mycoplasma by using MycoAlert Detection kit (Lonza, LT07-218). Cells were maintained at 37°C in a 5% CO2 incubator ...
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bioRxiv - Cancer Biology 2021Quote: ... and transferred to the well of a 16-well Nucleocuvette™ strip (SF Cell Line 4D-NucleofectorTM X Kit S, Lonza). Cells were electroporated on a 4D-NucleofectorTM X Unit (Lonza ...
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bioRxiv - Cell Biology 2019Quote: ... Feng and Coulombe 2015b) were transfected into Krt14-/- skin keratinocytes using P1 Primary Cell 4D-Nucleofector™ X Kit (V4XP-1024, Lonza). After nucleofection ...
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bioRxiv - Cell Biology 2019Quote: ... was transfected into skin keratinocytes in primary culture using the P1 Primary Cell 4D-Nucleofector™ X Kit (V4XP-1024, Lonza).
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bioRxiv - Cell Biology 2020Quote: ... The cells were tested for mycoplasma on receipt of the cell line and quarterly thereafter using the MycoAlert Mycoplasma Detection Kit according to the manufacturer’s instructions (Lonza, Basel, Switzerland).
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bioRxiv - Molecular Biology 2021Quote: ... Cells were electroporated with enhancer (IDT, #1075915, final concentration 4 μM) in a nucleofector device (Lonza, Nucleofector 2; program D-032). Cells were incubated for 24 h to allow them to recover and then detached and cloned by serial dilution ...
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bioRxiv - Molecular Biology 2022Quote: ... The Cas9-sgRNA mixture was added to the cells to a final volume of 20 μL and transferred to 16-well Nucleocuvette™ strips (Lonza). Pulse was applied utilizing the CM-130 program for HEK293 cells and EA-104 for RPE1 cells ...
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bioRxiv - Immunology 2022Quote: ... The concentration of IL-2 was increased to 1000 IU/ml on day 7 and the expanded T cells were electroporated with the indicated mRNA at a concentration of 2 pg mRNA/cell on day 8 using 4D Nucleofector™ System (Lonza) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: BMDMs (1 × 106 cells) were transfected with siRNA (250 pmol) and Amaxa Mouse Macrophage Nucleofector Kit (Lonza, catalog number VPA-1009) using a Lonza Nucleofector 2b device (Lonza ...
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bioRxiv - Microbiology 2019Quote: ... the antibody-virus mixture was aspirated and Vero cells were washed with PBS and overlaid with DMEM containing 2% heat-inactivated FBS and 1% SeaPlaque Agarose (Lonza, 50501). After 4–6 days ...
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bioRxiv - Genomics 2019Quote: ... The complex was then transferred to a 20 μl single-cell suspension of 2 × 105 hESCs in P3 nucleofection solution and electroporated using Amaxa 4D-Nucleofector™ (Lonza) with program CA137 ...
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bioRxiv - Genomics 2021Quote: ... Four aliquots of one million cells each per study subject were stimulated overnight for 12 hours in 1 ml X-VIVO™ 15 Serum-free Hematopoietic Cell Medium (Lonza) with 10 ul ImmunoCult™ Human CD3/CD28/CD2 T Cell Activator (STEMCELL Technologies) ...
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bioRxiv - Genomics 2021Quote: ... Cell lines were authenticated by STR profiling and mycoplasma testing was performed using the MycoAlert™ PLUS Mycoplasma Detection Kit (Lonza). U2OS cells were grown in DMEM supplemented with 10% tetracycline-free foetal bovine serum (Clontech) ...
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bioRxiv - Cancer Biology 2021Quote: ... expressing the human CD133 antigen45 was co-delivered with a PBase expression vector (2.5 μg) into 2 ⨯ 106 U251 cells using Nucleofector 2b (Lonza, Köln, Germany), followed by puromycin (500 ng/ml ...
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bioRxiv - Bioengineering 2020Quote: 2 million UCB-MNC/mL were cultured in X-VIVO 15 serum-free cell-culture medium (Lonza Group Ltd, Basel, Switzerland), supplemented with 0.5 μg/mL of FMS-like tyrosine kinase-3 and 0.5 μg/mL of stem-cell factor ...
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bioRxiv - Neuroscience 2020Quote: ... All cells were routinely tested for mycoplasma contamination with MycoAlert Mycoplasma Detection Kit (Lonza, Rockland, ME, USA, Cat N° LT07-318). Chemicals and cell culture reagents were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Cancer Biology 2020Quote: ... dissociated into single cell suspension with trypsin-EDTA (Pan-Biotech, Germany) for 3 min followed by trypsin neutralizing solution (Lonza, Germany). Single cell suspensions of secondary mammospheres were obtained as described for day 7-first generation mammospheres.
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bioRxiv - Cancer Biology 2019Quote: 2.106 cells were electroporated with double-stranded siRNA to a final concentration of 2µM using an electroporation device (Lonza 4D Nucleofector) according to the manufacturer’s specifications ...
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bioRxiv - Cell Biology 2021Quote: ... HeLa cells were transfected with the enzyme and with PAK1 or PAK2 sgRNA using nucleofection (4D-Nucleofector™ X Unit, Lonza). Due to low transfection efficiency in leukemia cell lines ...
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bioRxiv - Immunology 2021Quote: ... The infected cells were incubated for 72 h in Dulbecco’s Modified Eagle Medium with L-glutamine (DMEM; Lonza, Walkersville, MD, USA) containing 2% heat-inactivated fetal bovine serum (FBS ...
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Rag GTPases and Phosphatidylinositol 3-Phosphate Mediate Recruitment of the AP-5/SPG11/SPG15 ComplexbioRxiv - Cell Biology 2020Quote: ... The cell lines were routinely tested for the presence of mycoplasma contamination using MycoAlert™ Mycoplasma Detection Kit (LT07-318, Lonza), and were also regularly treated with mycoplasma removing agent (093050044 ...
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bioRxiv - Cancer Biology 2021Quote: ... in a total volume of 100μL/ reaction and electroporated using an Amaxa TM Basic Nucleofector TM Kit for Primary Mammalian Epithelial cells (Lonza, # VPI-1005) and the NucleofectorTM 2b Device (Lonza ...
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bioRxiv - Microbiology 2022Quote: ... the antiviral activity of stenoparib on SARS-CoV-2 was assessed in vitro using Vero E6 Cercopithecus aethiops kidney cells (cat # CRL-1586, ATCC, Manassas, VA, USA) maintained in Dulbecco’s Modified Eagle Medium (DMEM; cat #BE12-733F, Lonza, Basel, Switzerland) supplemented with 2 mM L-glutamine (cat # BE17-605E ...
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bioRxiv - Bioengineering 2022Quote: ... that were used to line the vascular channel of the chips were first expanded in microvascular endothelial cell growth medium (EGM™-2MV BulletKit, CC-3202; Lonza) in plastic tissue culture flasks (Falcon 353136 and 355001 ...
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bioRxiv - Developmental Biology 2022Quote: ... and all cell lines are routinely monitored for mycoplasma infection monthly using the MycoAlert Mycoplasma Detection Kit (Lonza, Cat#LT08-118). Stem cells were maintained as previously described (Spence et al. ...
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bioRxiv - Biophysics 2022Quote: ... Cells were negative for mycoplasma contamination and are regularly tested using the MycoAlert PLUS Mycoplasma Detection Kit (Lonza, Rockville, MD, USA). Unless stated otherwise ...
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bioRxiv - Genomics 2022Quote: mESC were transfected with sgRNA constructs using the P3 Primary Cell 4D-Nucleofector X Kit (V4XP-3024) and the Amaxa 4D Nucleofector™ system (Lonza). We used the transfection programme CG-104 ...
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bioRxiv - Cancer Biology 2021Quote: Primary human lung microvascular endothelial cells (HMVEC-L) were seeded at 5000 cells/cm2 in EBM-2 with 5% FBS and EGM-2 supplement (all products, Lonza Bioscience) until reaching complete confluence 14 ...
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bioRxiv - Microbiology 2022Quote: ... Dissociated cells were aspirated using a wide-bore pipette tip and to a tube containing ice-cold Trypsin Neutralization Solution (Lonza, CC5002); this was repeated to maximize cell collection ...
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bioRxiv - Cell Biology 2019Quote: ... DNA was transiently transfected into HEK SNAP-β2 β-arr-KO cells by electroporation with the Amaxa Nucleofector II device (Lonza). The included program Q-001 is designed for high expression levels in HEK cells and was used according to the manufacture’s protocol ...
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bioRxiv - Developmental Biology 2019Quote: ... 4 μg of the donor ssDNA and 3 μg of a puromycin resistance plasmid (pCAG-puroR) using the Mouse ES Cell Nucleofector™ Kit (Lonza) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2020Quote: ... All the primary GBM cell lines were checked periodically for mycoplasma contamination using the MycoAlertTM Mycoplasma Detection Kit (Lonza, Basel, Switzerland). All Patient-derived biological samples were collected according to a protocol approved by Italian Local Ethics Committee (CE IRST IRCCS-AVR ...
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bioRxiv - Biophysics 2019Quote: The HEK293 adherent culture cells were transfected with pcDNA3.4-PCV2d plasmid using Amaxa Nucleofector II instrument by Lonza (program A-23). The transfected cell were plated on 6-well plate ...
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bioRxiv - Cell Biology 2019Quote: To induce expression of fluorescently labeled proteins DCs were transfected according to manufacturer guidelines using nucleofector kit for primary T cells (Amaxa, Lonza Group). Briefly ...
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bioRxiv - Biochemistry 2019Quote: ... 1.5-2×106 cells were pelleted for each condition and resuspended in 100 μL complete nucleofector solution (SE Cell Line 4D-Nucleofector™ X Kit, Lonza) to which 1μg of pCR2.1-mClover-LMNAdonor ...
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bioRxiv - Cell Biology 2021Quote: ... MDCKII cells were transfected by electroporation with plasmids encoding CD4-SIV Env chimeras using an Amaxa Cell Line Nucleofector™ Kit L and Amaxa Nucleofector II with settings L-05 (Lonza). Stable transfectants were selected with 400 μg/ml G418 Sulphate (Calbiochem) ...
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bioRxiv - Cell Biology 2021Quote: ... All cell lines used in this study were monitored for mycoplasma infection using the MycoAlert Mycoplasma Detection Kit (Lonza TL07-218).