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Citations for Lonza :
251 - 300 of 2288 citations for 3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: Human telomerase-immortalized corneal epithelial (hTCEpi) cells (26) were maintained at 37°C/5% CO2 in regular keratinocyte growth medium KGM-2 (Lonza). Prior to treatment ...
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bioRxiv - Immunology 2021Quote: ... CD3/CD28 beads were removed 48 hours after stimulation and 5 × 106 CTLs were electroporated with 2 μg plasmid using 4D-Nucleofector (Lonza). Medium was changed 6 hours after nucleofection and transfected cells were used 24-36 hours after electroporation ...
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bioRxiv - Developmental Biology 2022Quote: ... Primary MEC were cultured in endothelial growth media (EGM) containing 5% FBS with growth factors (EBM-2; Lonza, Basel, Switzerland) at 37°C in 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... cells (5×105/well in 6-well plates) were transfected with 2 μg of DNA by nucleofection using Amaxa device (Lonza) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... the cell pellet was resuspended in 1-5 ml ACK lysis buffer (Lonza), according to the pellet size ...
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bioRxiv - Bioengineering 2020Quote: ... The cell suspension contained a 2:1 ratio of GFP-HUVECs (Lonza) and HDFs ...
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bioRxiv - Molecular Biology 2024Quote: ... ∼300 pairs of ovaries were dissected from 3-6 day old females in 1× PBS (Lonza) with 1 mM DTT (Sigma ...
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bioRxiv - Synthetic Biology 2021Quote: ... Isolated NK cells were activated at 1 x 106 cells mL-1 for 5 days in XVivo15 medium (Lonza) with 5% fetal bovine serum ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were maintained at 37°C in 5% CO2 in either EGM-2 MV complete media (CC-3125, Lonza, Walkersville, MD) or in M199 supplemented with 20% FBS (Atlanta Biologicals ...
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bioRxiv - Cell Biology 2024Quote: ... These cells are mantained in 30% FBS/1% antibiotic/antimycotic /1% Non-essential AA/ EGM-2 media (Lonza) with Laminin521 coating (5 μg/ml ...
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bioRxiv - Genomics 2020Quote: ... The first consisted of 2 μl/well pmaxGFP DNA (LONZA,1 μg/ul) diluted in 250 μl/well Opti-MEM® medium (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... and 8 × 105 cells were electroporated using 5 mg of total DNA (Ratio 1:1) and the Amaxa Nucleofector kit (Lonza) as described (Ran et al. ...
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bioRxiv - Genetics 2024Quote: ... Cells were cultured at 37°C in a humidified atmosphere and 5% CO2 in a 1:1 mixture of DMEM (Lonza) and Ham’s F10 (Lonza ...
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bioRxiv - Cell Biology 2022Quote: ... and CD31+Rspo3+ cells were centrifuged at 500 g for 5 min and resuspended in 500 μl endothelial cell media: EGM-2 MV Bullet Kit (Lonza, cc-3202) supplemented with 50 ng/mL VEGF-C (R&D ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were grown at 37°C with 5%CO2 using EGM-2 medium supplemented with SingleQuots from Lonza (CC-3156 & CC-4176). Cells were passaged using 0.25% trypsin EDTA every 2–3 days ...
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bioRxiv - Bioengineering 2020Quote: ... Fluidic lines were coated with 1% gelatin which was replaced by EGM-2 (Lonza) for up to 24 hours ...
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bioRxiv - Cell Biology 2023Quote: HUVECs were grown on 1% gelatin-coated plates in EGM-2 medium (Lonza: CC3156) for 48 hrs ...
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bioRxiv - Bioengineering 2023Quote: ... in 6-well tissue culture plates and were allowed to adhere for 5 hours before induction of serum starvation in EBM-2 (LONZA, Cat# CC-3156) for 16 hours prior to being administered media of interest ...
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bioRxiv - Developmental Biology 2021Quote: ... and E15.5 (n=2–6) Six2-TROMA-1 stained kidneys were embedded in 1 % low melting agar (50100; Lonza Group). The lateral cortex of the kidneys was imaged with a Nikon A1R MP+ multiphoton microscope (Tokyo ...
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bioRxiv - Immunology 2021Quote: Commercially available human primary PTs from 6 donors (3 males and 3 females, Lonza Walkersville Inc) were expanded at passage 4 ...
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bioRxiv - Microbiology 2022Quote: ... 2 ml of 2% SeaPlaque™ Agarose (Lonza) in DMEM containing 2% FBS and 1% penicillin/streptomycin (P/S ...
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bioRxiv - Microbiology 2021Quote: ... 2◻ml of 2% Seaplaque agar (Lonza) in Dulbecco’s modified Eagle medium (DMEM ...
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bioRxiv - Microbiology 2021Quote: ... 2◻ml of 2% Seaplaque agar (Lonza) in DMEM containing 2% FBS ...
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bioRxiv - Biophysics 2021Quote: ... HUVECs (Lonza, expanded to passage 3) were transduced with GFP tagged VE-cadherin (65 ...
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bioRxiv - Synthetic Biology 2021Quote: ... and γδ T cells were activated at 1 x 106 cells mL-1 for 2 days in complete XVivo15 medium (Lonza) (5% fetal bovine serum ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were infected with virus dilutions (from 10-1 to 10-9) prepared in DMEM supplemented with 2% FBS and 1%Penicillin/Streptomycin mixture (Lonza Biosciences). Infected cells were incubated at 37°C for 7 to 15 days ...
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bioRxiv - Cancer Biology 2023Quote: All cultures were routinely (every 1-2 months) tested for mycoplasma contamination using MycoAlert (Lonza). Autosomal STR profiles for cell line authentication were performed by the University of Arizona Genetics Core.
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bioRxiv - Bioengineering 2020Quote: ... HUVECs were cultured in Endothelial Growth Medium 2 (EGM-2) and NHLFs were cultured in Fibroblast Growth Medium 2 (FGM-2) (Lonza, Walkersville, MD). All cells were cultured in incubators at 37 °C and 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... plates were incubated at 37°C for 30 minutes in blocking buffer (PBS with 1% BSA, 5% FBS, and 1% human AB serum (Lonza, Walkersville, MD, USA), washed three times in PBS with 0.05% tween 20 and incubated with a commercial anti-SARS-CoV-2 Spike antibody (1/1000 ...
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bioRxiv - Bioengineering 2021Quote: ... were purchased from Lonza and cultured in EGM-2 and FGM-2 (Lonza) supplemented with EGM-MV and FGM-2 Bullet Kit ...
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bioRxiv - Bioengineering 2022Quote: ... Endothelial Cell Growth Medium-2 (EGM-2, Lonza, Switzerland) was used as the medium ...
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bioRxiv - Genomics 2022Quote: ... Following 6-TG treatment 5×106 HM-1 cells were transfected using a Nucleofector®-4D (Lonza) with the P3 Primary Cell X kit ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Measured tissues originated from six F508del homozygous patients (CF patient #1: CF-AB0609, female, 31 years-old; CF patient# 3: #28388-0000450918, Lonza, male ...
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bioRxiv - Cell Biology 2024Quote: ... together with a plasmid encoding the piggyBac transposase in a 3:1 ratio (vector:transposase) using the P3 Primary cell 4D-Nucleofector™ kit (Lonza). Prior to targeting ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells were first electroporated with a PiggyBac-configured plasmid containing the Dox-inducible NbALFA-ABEL degrader cassette and a plasmid encoding the PiggyBac transposase at a 3:1 mass ratio via nucleofection (solution E with program CM138) according to manufacturer’s instructions (Lonza Inc.), followed by puromycin selection (5μg/ml ...
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bioRxiv - Synthetic Biology 2024Quote: ... The optimised sequences were used to design gBlocks with appropriate overhangs (Fig. 1-3) and cloned into pMAX-GFP plasmid from Lonza, digested with KpnI and SacI (Fig ...
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bioRxiv - Bioengineering 2024Quote: SP8 or DP T cells were isolated as described above and stimulated in vitro using irradiated K562 CD19-CD137L aAPCs at a 1:3 aAPC:T cell ratio in X-VIVO™ 15 (Lonza), supplemented with 5% human AB serum (Cat ...
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bioRxiv - Bioengineering 2022Quote: ... and 3% FBS (all from Lonza Biosciences), termed astrocyte medium ...
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bioRxiv - Bioengineering 2022Quote: Endothelial cords were cultured in EGM-2 (EBM-2 with the addition of EGM-2 BulletKit) (Lonza). For most experiments ...
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Cardiac pericytes are necessary for coronary vasculature integrity and cardiomyocyte differentiationbioRxiv - Physiology 2022Quote: ... were cultured in endothelial basal medium-2 (EBM-2) supplemented with EGM™-2 BulletKits™ (Lonza). Cells at passage 3 were used.
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bioRxiv - Physiology 2020Quote: ... were cultured in endothelial basal medium-2 (EBM-2) supplemented with EGM™-2 BulletKit™ (Lonza). Cell from passage 3 to passage 6 were used ...
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bioRxiv - Bioengineering 2020Quote: ... and 40 % EGM-2 (EBM-2 Lonza CC-3156; Clonetics) formed the basic medium ...
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bioRxiv - Bioengineering 2022Quote: ... were obtained from Lonza and cultured in Endothelial Growth Medium-2 (EGM-2, Lonza) or EGM-2 BulletKit (Lonza) ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured in EBM-2/EGM-2 medium (Lonza) with 12% FBS ...
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bioRxiv - Immunology 2020Quote: ... coated plates in Keratinocyte Growth Medium 2 (KGM-2, Lonza) at a density of 2 x 105 cells/cm2 and cultured at 37°C at 5% CO2 ...
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bioRxiv - Evolutionary Biology 2021Quote: For agarose/stiffness preference assays, experimental substrates contained 75mM sucrose, 1% ethanol and increasing concentrations (1%, 1.5%, and 2%) of agarose (Lonza SeaKem LE Agarose #50001); control substrates were 0.25% agarose and contained 75mM sucrose and 1% ethanol.
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bioRxiv - Neuroscience 2022Quote: ... Homogenized samples were then diluted 1:5 in endotoxin free water in pyrogen-free glass tubes (Lonza N207) and tested for endotoxin in duplicate using a Kinetic-QCL™ LAL Assay (Lonza ...
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bioRxiv - Microbiology 2021Quote: ... Vero cell cultures were seeded at 1-3 × 104 cells/cm2 in Dulbecco’s minimal essential medium (DMEM, LONZA, Alpharetta, GA, USA) supplemented with 9% foetal bovine serum (FBS ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and PX458-AAVS1 (3 µg) were electroporated into 1.3×106 H9 cells using the Human Stem Cell Nucleofector Kit 1 (Lonza, VPH-5012). Following electroporation the cells were seeded across 3-wells of a 6 well plate coated with Matrigel in StemFlex supplemented with RevitaCell supplement (Thermo Fisher ...
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bioRxiv - Cell Biology 2022Quote: ... They were cultured in EC specific medium with 2% FBS (EGMTM-2 EC Growth Medium-2 BulletKitTM, LONZA® or EC Growth Medium 2, PromoCell®) at 105 cell/well in a 6 well plate ...