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Citations for Lonza :
51 - 100 of 2288 citations for 3 Hydrazino 5 methyl 4H 1 2 4 triazol 4 ylamine hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... and electroporated with 2-4pmol HDR template using a 4-D Nucleofector with pulse code CM-150 (Lonza). VLP/template mix was added to 15,000 cells in 50ul DMEM +10% FBS and 1x penicillin/streptomycin.
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bioRxiv - Molecular Biology 2023Quote: ... ∼2-4 million cells were electroporated using the T-020 method of the Nucleofector™ 2b device (Lonza). Immediately after electroporation ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were harvested with trypsin, pelleted (500 g, 5 min, 4°C) and resuspended in PBS (pH 7.4) containing EDTA (Lonza) and 10% fetal bovine serum ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were harvested with trypsin, pelleted (500 g, 5 min, 4°C) and resuspended in PBS (pH 7.4) containing EDTA (Lonza) and 10% fetal bovine serum ...
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bioRxiv - Immunology 2022Quote: ... and 4 mM of L-glutamine (Lonza). HLA-E expressing K562 cells were sorted and maintained in complete RPMI supplemented with 2 µg/mL blasticidin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... Human primary hepatic stellate cells from donors 3 and 4 were purchased as isolated hepatic stellate cells from Lonza (cat# HUCLS). Donor information is listed below.
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bioRxiv - Microbiology 2020Quote: ... 10 μg of RNA were fractionated on a 2% NuSieve 3:1 agarose (Lonza), 1X MOPS ...
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bioRxiv - Microbiology 2023Quote: ... 10 μg of RNA were fractionated on a 2% NuSieve 3:1 agarose (Lonza), 1X MOPS ...
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bioRxiv - Microbiology 2024Quote: ... 10 μg of RNA were fractionated on a 2% NuSieve 3:1 agarose (Lonza), 1X MOPS ...
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bioRxiv - Genetics 2020Quote: ... 20 µg ssODNs and 4 µg pSpCas9(BB)-2A-GFP construct using Human Stem Cell Nucleofector Kit 2 (Lonza). For the generation of UE-RASGEF1A-int1-KO and PIK3C2B-int10-KO hPSC lines ...
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bioRxiv - Pathology 2024Quote: Primary human lung fibroblasts from either IPF patients (n=4) or donors with no history of lung fibrosis (n=5) were purchased from Lonza at P2 (Supplementary Table 6) ...
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bioRxiv - Immunology 2020Quote: ... Transfection of primary T cells with Cas9 RNP complexes and TCRβα HDR templates was performed 3-4 days following activation using the 4D-Nucleofector and a 20 uL format P3 Primary Cell kit (Lonza, V4XP-3032). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... 4 μM Alt-R Cas9 Electroporation enhancer (IDT) and 4 μM single-strand DNA homology template (Ultramer DNA Oligonucleotide, Lonza). After electroporation ...
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bioRxiv - Neuroscience 2021Quote: ... a 4% agarose (SeaPlaqueTM GTGTM Agarose, 50111; Lonza) solution was prepared in 0.1M PBS and left at 45°C in a dry bath ...
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bioRxiv - Biochemistry 2020Quote: One straw of passage 4 HUVECs (Lonza, USA) was thawed in a 35mm dish and cultured in EGM-2 medium until almost 80% confluence is reached ...
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bioRxiv - Bioengineering 2022Quote: ... supplemented with 4 mmol/L L-glutamine (Lonza), 100 IU/ml Penicillin/100 mg/ml Streptomycin (Lonza) ...
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bioRxiv - Neuroscience 2021Quote: ... containing 4% low melting point agarose (50111, Lonza) which was then allowed to solidify at 4°C for a few minutes ...
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bioRxiv - Bioengineering 2020Quote: Passage 4 human mesenchymal stem cells (Lonza, Switzerland) were expanded to passage 5 in an incubator set at 37°C and 5% CO2 and fed with low glucose DMEM and glutamine ...
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bioRxiv - Cancer Biology 2020Quote: ... and embedded in 4% low-melting agarose (Lonza) preheated at 42 °C ...
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bioRxiv - Microbiology 2023Quote: ... 4 x 104 in 0.4 mL MEM (Lonza), 10% FBS (Corning) ...
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bioRxiv - Genomics 2022Quote: ... 4 million cells were electroporated (Amaxa Nucleofection, Lonza) with 25μg of L1-Cag/FLAG_NLS_ORF-1L plasmid and 15μg of pIC-Cre ...
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bioRxiv - Cell Biology 2023Quote: ... or Melanocyte Growth Basal Medium-4 (Lonza, Switzerland) after pigmented cells were dominant.
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bioRxiv - Cancer Biology 2022Quote: A small fragment (2-4 mm3) of tumor biopsies or surgically resected tumor was cultured in RPMI 1640 plus (Lonza) containing 10% FBS Hyclone (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... for 24 h then interleukin-2 (50U/ml) for 4-6 days before nucleofection (as recommended by the manufacturer, Lonza) or infection by HIV-1.
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bioRxiv - Neuroscience 2021Quote: ... the suspension was centrifuged (215g for 5min at 4°C) and the pellet was resuspended in BMEC-media that comprised of EBM-2 medium (Lonza) supplemented with the following ...
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bioRxiv - Cancer Biology 2020Quote: ... CHO-S cells were resuspended at a density of 4 mio cells/mL in ProCHO 4 medium (Lonza, #LZ-BE12-029Q) supplemented with 8 mM ultraglutamine (Lonza ...
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bioRxiv - Biophysics 2021Quote: ... The WT and mutant genes were then amplified from these expression plasmids using the PCR primers 5’-GCGCTAGCAGCACCCATATGCCTGAG-3’and 5’-ACGAAGCTTTCAGTGGTGGTGGTGGT-3’and subcloned into the pMaxCloning vector (Lonza, VDC-1040) via NheI and BamHI sites to generate the pMax_WT_NEIL1 and pMax_Mutant_NEIL1 expression plasmids that were utilized throughout this analysis.
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bioRxiv - Molecular Biology 2023Quote: ... A cell count ranging from 1 × 10^5 to 2 × 10^5 cells was collected and suspended in 15 µL of nucleofection buffer (Lonza). Electroporations were conducted in the strip format ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were electroporated with enhancer (IDT, #1075915, final concentration 4 μM) in a nucleofector device (Lonza, Nucleofector 2; program D-032). Cells were incubated for 24 h to allow them to recover and then detached and cloned by serial dilution ...
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bioRxiv - Cell Biology 2021Quote: ... and cells were isolated by centrifugation at 500 g for 5 min at 4°C followed by being treated with ACK Lysing Buffer (10-548E, Lonza Bioscience, Switzerland) to remove red blood cells ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were electroporated at 5 million cells/100 mL cells per cuvette using a Lonza 4-D nucleofector with pulsecode DP-148 (Lonza, VVPA-1002). Cells were co-cultured for 5 additional days with human astrocyte before transferring cells to homeostatic culture conditions (MGdM media ...
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bioRxiv - Cell Biology 2021Quote: ... M206 and M443 were cultured in (MGM-4, Lonza) supplemented with 1 ml CaCl2 ...
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bioRxiv - Cancer Biology 2024Quote: ... and cultured in MGM-4 melanocyte growth medium (Lonza) + 200 nM 12-O-tetradecanoylphorbol-13-acetate (TPA ...
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bioRxiv - Genomics 2020Quote: ... Human umbilical vein endothelial cells were isolated from umbilical cords of healthy donors after cesarean sections (HUVECS; n = 4; RWTH Aachen) (55) or obtained from Lonza (n = 3, Basel, Switzerland) (8) ...
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bioRxiv - Physiology 2022Quote: ... (5 g/L trypsin and 2 g/L EDTA diluted 1 in 5 in calcium- and magnesium-free PBS) (Lonza, UK).
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bioRxiv - Immunology 2022Quote: ... were coated overnight at 4°C with 25μL of SEC fractions 1 to 5 diluted 1:2 with PBS (Cat. No. LZBE17-512F, Lonza). Wells were washed with 0.05% Tween-20 (Cat ...
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bioRxiv - Cell Biology 2020Quote: Electroporation was performed using an Amaxa 4-D device (Lonza) or a Neon Transfection System (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 days after nucleofection with the AMAXA Nucleofector Kit (Lonza), we applied puromycin selection until we observed the appearance of green colonies ...
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bioRxiv - Developmental Biology 2020Quote: ... embedded in 4% low melting temperature agarose (Lonza, Cat # 50100) and sectioned in a vibratome to produce 300μm-thick coronal slices ...
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bioRxiv - Neuroscience 2024Quote: ... and mounted in 4% low melting point agarose (50100, Lonza) for sectioning (70 μm ...
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bioRxiv - Bioengineering 2023Quote: ... Normal human dermal fibroblasts (NHDFs; Lonza; passages 4 to 10) were cultured on dishes in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Cell Biology 2024Quote: Human umbilical vein ECs (HUVECs, passage 4-6, pooled, Lonza) were cultured in EC growth medium-2 (EGM™-2 Bulletkit™ ...
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bioRxiv - Microbiology 2021Quote: ... 2% glutamine and 5% human serum AB (Lonza) for seven days ...
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bioRxiv - Cell Biology 2021Quote: ... 5% C02 in EGM-2 (Lonza, #CC-3162), media was changed every two days and cultured to passage 6 ...
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bioRxiv - Bioengineering 2023Quote: ... Adipose-derived stem cells (Passage 3-5) (Lonza, Walkersville, MD, USA) were cultured in a basal medium consisting of DMEM/F12 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 52 years-old; non-CF donor #2: WT-AB0834, male, 71 years-old; non-CF donor #4: CC-2540S-20TL256517, Lonza, female, 48 years-old). After co-incubation with correctors for 24 h in medium (MucilAir™ medium ...
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bioRxiv - Neuroscience 2022Quote: ... pCE-hOCT3/4 and pCE-mp53DD using the Amaxa Nucleofactor (Lonza). The cells were then cultured in TeSR-E7 medium for 3-4 weeks on Matrigel-coated dishes (Corning ...
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bioRxiv - Biophysics 2021Quote: ... in passages 4-8 were cultured in EGM2-MV medium (Lonza) at 37°C in a humidified atmosphere of 95% air and 5% CO2 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... non-CF#4 (CC-2540S -20TL356517, Lonza, female, 48 years-old). hAEC cells were cultured for several days until reaching 90-95% confluency in complete PneumaCultExPlus medium ...
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bioRxiv - Immunology 2023Quote: ... embedded in 4% low melting point agarose (GTG-NuSieve Agarose, Lonza) and sectioned into 500 μM slices using a vibratome (VT1000S ...