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Citations for Lonza :
1801 - 1850 of 2037 citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... 5 × 107 hepatocytes were suspended in 30 mL of HCM™ (Lonza, Sales Ltd, Basel, Switzerland) and injected via the bile duct at a flow rate of 1 mL/min ...
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bioRxiv - Immunology 2020Quote: ... the cells were resuspended in 5 µL nucleofection solution P1 or P3 (with supplement added, Lonza) per 4 · 105 cells ...
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bioRxiv - Cell Biology 2023Quote: Drosophila ovaries (5-8 pairs per sample) were dissected into room temperature Grace’s insect medium (Lonza). Ovaries were fixed for 10 min using 4% paraformaldehyde diluted in Grace’s medium ...
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bioRxiv - Bioengineering 2024Quote: ... 5 million cells were suspended in 74 μl of P4 Primary Cell solution (Lonza, VSOP-4096). In parallel ...
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bioRxiv - Bioengineering 2022Quote: ... 20 μL of T cells were resuspended in P3 buffer at 5 × 107 cells/mL (Lonza) and added to the electroporation mixture ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids and siRNAs were introduced into chromaffin cells (5 × 106 cells) by Amaxa Nucleofactor systems (Lonza) according to manufacturer’s instructions and as described previously [46] ...
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bioRxiv - Cell Biology 2020Quote: 1 million Jurkat cells were electroporated with the 2.5 μg of sgRNA plasmid and GFP control plasmid at a 10:1 ratio using an Amaxa Cell Line Nucleofector Kit V and an Amaxa™ Nucleofector™ II (Lonza). HeLa ...
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bioRxiv - Immunology 2021Quote: ... 1.5×106 cells multiplied by the number of mice to be injected were transfected with the pIL-10 vector (1×106 cells/1.5 μg pIL-10 DNA per cuvette) using the Mouse T Cell Nucleofector Kit (Lonza, No: VPA-1006) with Nucleofector II Device (program X-100) ...
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bioRxiv - Molecular Biology 2021Quote: ... and 199 (M199) medium (3: 1) supplemented with 10% fetal bovine serum (FBS),10 mM glutamine and penicillin-streptomycin (all from Lonza, Basel, Switzerland) and their purity was verified with the fibroblast marker TE-7 (Merck ...
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bioRxiv - Cancer Biology 2021Quote: ... To generate IKZF3-deficient CAR-T cells, 15-20 million PBMCs in RPMI 1640 (10% FBS, 1% penicillin/streptomycin) were nucleofected using Nucleofector 2b (Lonza, Köln, Germany) with a mixture of 4 plasmids (5 μg each ...
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BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... complex with a ratio of 4.5 to 1 between sgRNA and Cas9 was delivered following the protocol of the SE Cell Line 4D-NucleofectorTM X Kit (Lonza, V4XC-1012), using the nucleofection program DS-130 on the Lonza 4D X unit ...
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bioRxiv - Cell Biology 2021Quote: ... 500μg of gRNA and 500μg of donor plasmids were electroporated in 1×106 mESC using P3 Primary Cell 96-well Nucleofector™ Kit (Lonza, PBP3-22500) according to manufacturer’s instruction ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were electroporated with 10 µg lineage recorder DNA and 1 µg Sleeping Beauty transposase in 100 µl Human Stem Cell nucleofection buffer (Lonza, VVPH-5022) using the B-016 program of the 4D-Nucleofector (Lonza ...
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bioRxiv - Biophysics 2022Quote: ... 106 U2OS cells were transfected with 1 μg of px330 plasmid and 1 μg of the HDR donor plasmid using the Nucleofector 2b device and cell line nucleofector kit V (Lonza, VCA-1003) per manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... 20 pmol Cas9 nuclease (sgRNA to Cas9 nuclease ratio used as 9:1) was performed with Amaxa 2D Nucleofector (Lonza, program B016). After recovery ...
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bioRxiv - Bioengineering 2022Quote: ... supplemented with 10% (v/v) fetal bovine serum (FBS, Biochrom AG, Berlin, Germany) and 1% (v/v) antibiotic–antimycotic solution (AAS, Lonza, Basel, Switzerland) was used for culturing human dermal fibroblasts (HDF ...
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bioRxiv - Cancer Biology 2020Quote: ... 10 mmol/L HEPES (PAA Laboratory) and 1 ng/mL human basic fibroblast growth factor (bFGF) (Sigma-Aldrich) (Lonza, Walkersville, MD, USA). The generation number of ECs is maintained between 30-40 generations ...
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bioRxiv - Immunology 2020Quote: ... and CDR3B gRNA (10 nmol) were used to transfect 1×108 TnT cells using the 4D-Nucleofector LV unit (Lonza, #AAF-1002L). TnT cells with restored CD3 surface expression were bulk-sorted (SEL 1 ...
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bioRxiv - Microbiology 2021Quote: ... were isolated by Ficoll density gradient centrifugation as described previously [22] and seeded at a density of 1×106 cells/cm2 in X-VIVO 15 medium (Lonza, Cologne, Germany) supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were transfected with pcDNA3.1 empty vector or pcDNA3.1 containing the human coding sequence of the VGLL2-NCOA2 fusion using the AMAXA cell line Nucleofection kit V (Lonza #VCA-1003;) and were selected in growth media containing 1μg/ml concentration of G418 (ThermoFisher #10131035) ...
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bioRxiv - Genomics 2023Quote: ... 200,000 cells were nucleofected with 50 fmol transposon and 50 fmol transposase (Super piggyBac Transposase - SystemBio PB210PA-1) or with 300 ng pmaxGFP using the P2 Primary Cell 4D Nucleofector kit (Lonza V4SP-2096) and the Lonza 4D- Nucleofector with the DS-150 program ...
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bioRxiv - Cell Biology 2023Quote: ... RPE1 cell pellets containing 1×10^6 cells were resuspended in Nucleofection Solution for Primary Mammalian Epithelial Cells (Lonza cat# VPI-1005) in the presence of nucleofection enhancer (cat# 1075915 ...
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bioRxiv - Systems Biology 2022Quote: ... The PGK reporter cell line was generated by electroporation of K562 cells with 0.5 ug each of plasmids encoding the AAVS1 TALENs and 1 ug of donor reporter plasmid using program T-016 on the Nucleofector 2b (Lonza, AAB-1001). Cells were treated with 0.5 ug/mL puromycin for one week to enrich for successful integrants ...
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bioRxiv - Genetics 2024Quote: ... erythroleukemia K562 cells were washed in 50 mL PBS at RT and approximately 1×106 cells were resuspended in SF solution (Lonza V4XC-2032) and electroporated in presence of pre-assembled Cas9:gRNA complexes plus 12 µg ssDNA (100 µL format ...
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bioRxiv - Cell Biology 2024Quote: ... the Ribonucleoprotein (RNP) complexes (9:1 sgRNA to Cas9 ratio) were assembled in Nucleofector Solution plus Supplement (Lonza Amaxa HMEC Nucleofector Kit) to a total volume of 100µL ...
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bioRxiv - Genomics 2024Quote: ... 200,000 cells were nucleofected with either 500 ng pJK028A and 500 ng pJK029A or 1 μg pJKEB01 using the Lonza SF Cell Line 4D-Nucleofector X Kit S (Lonza, V4XC-2032) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... after thaw for about 1 week and tested for mycoplasma regularly using the MycoAlertTM PLUS Mycoplasma Detection Kit (Lonza, CAT#: LT07-318). Cells were always used within 10 passages of authentication via STR analysis with CellCheck9 by IDEXX laboratories.
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bioRxiv - Immunology 2024Quote: ... One million NK cells were then resuspended in 20 µl of 4D Nucleofector Master Mix (82% P3 solution + 18% Supplement 1; Lonza, V4XP-3032) and mixed with the Cas9 RNP complex for electroporation using the CM137 program ...
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bioRxiv - Cancer Biology 2024Quote: Orthotopic tumors were induced by injecting subcutaneously 1 x 106 4T1 cells in 50 µL of PBS (Lonza, catalog no. 17-512F) into 4th mammary fat pad of 8-12-week-old female Balb/c mice ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 × 105 OVCAR8 landing pad cells were resuspended in 20 µl of nucleofector solution combined with supplement at 4.5:1 from the Amaxa SE Cell Line 4D-Nucleofector X Kit S (Lonza, Cat #V4XC-1032), before addition of 5 µl RNP complex and 1 µl of Electroporation enhancer ...
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bioRxiv - Developmental Biology 2024Quote: ... plasmids PB001 carrying the mCherry cassette and pBase carrying the transposase (2 μg of each plasmid) were electroporated into 10 million H1 or E14 cells using the Human Stem Cell Nucleofector Kit 1 (Lonza, VPH-5012). Following electroporation ...
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bioRxiv - Cell Biology 2020Quote: ... cells were serum starved for at least 13 h in 1% FBS (Atlanta Bio selected, Cat #S11110) or without FBS using endothelial cells growth basal medium (Lonza cat#cc-3121) instead of MCDB-131-complete media ...
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bioRxiv - Neuroscience 2020Quote: ... two pairs of sgRNA vectors and Cas9 nickase plasmid were transduced into the AD hPSCs by using Human Stem Cell Nucleofector® Kit 1 (Lonza VPH-5012). Three days after electroporation ...
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bioRxiv - Neuroscience 2022Quote: ... The animal was then placed in a customized 35mm petri dish with sylgard-carved imaging chamber and covered with 1% low-melting agarose (Lonza #50101, Rockland, ME) for extra immobilization ...
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bioRxiv - Microbiology 2021Quote: ... NHBE cells were seeded in 24-well plates at 1×105 cells in a final volume of 0.5 ml BEGM™ Bronchial Epithelial Cell Growth Medium (BulletKit™; Lonza, Basel, SW). Cultures were incubated overnight 37 °C with 5% CO2 ...
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bioRxiv - Microbiology 2022Quote: ... Each pellet was carefully resuspended in 20 μL of SF Cell Line Nucleofector Solution with Supplement 1 (Lonza Cat. #V4XC-2032, Basel, Switzerland) and 1.5 μL of pX458 plasmids (150 ng each of BAX g2 ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1-5x105 iPSCs were resuspended in 20μL of P3 primary cell nucleofector solution plus supplement (cat.: V4XP-3032; Lonza Bioscience, Walkersville, MD, USA) along with the RNP complex and nucleofected using Lonza 4D Nucleofector (program CA-137) ...
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bioRxiv - Genetics 2024Quote: ... were transfected with 1 µg of plasmid DNA via electroporation using an AMAXA nucleofector kit following the manufacturer’s recommendation (Lonza Group Ltd., Basel, Switzerland). Plasmids included the empty pIRES-CD8 plasmid and pIRES-CD8 plasmids containing wild type MCOLN3 (MCOLN3WT ...
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bioRxiv - Cell Biology 2024Quote: ... and 5 μM of single-stranded donor oligonucleotides (ssODN) were delivered to 1×106 single cells by nucleofection using Lonza 4D nucleofection system (#V4XP-3024; Lonza Biologics, Slough, UK) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: Leukocyte transmigration assay was carried out by addition of 5×106 cells/mL hPBMC (#CC-2702 Lonza) to DENV2-infected HUVEC monolayers at 24 hpi following different treatments ...
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bioRxiv - Immunology 2022Quote: Cryopreserved PBMC (5 × 106/sample) were thawed in prewarmed RPMI-1640 with L-glutamine (Lonza, Basel, Switzerland) + 10% FCS ...
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bioRxiv - Bioengineering 2020Quote: ... cells were washed with 5 mL of 1X-phosphate buffered saline without magnesium and calcium (PBS) (Lonza) and supplemented with fresh DMEM-HEPES supplemented with 10 mM sodium butyrate (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2021Quote: ... Passaging of iPSCs was carried out every 4-5 days using Versene (EDTA 0.02%) (Lonza, BE17–771E) solution for 3–5⍰minutes at 37⍰°C ...
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bioRxiv - Immunology 2021Quote: ... Splenocyte suspension was spun down (1250 rpm, 5 min, 4C) and lysed in ACK Lysis buffer (Lonza) for 5 minutes on ice ...
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bioRxiv - Genomics 2022Quote: ... 5 μg of LMNA-VRQRABE-sgRNA was mixed in a total 25 μL volume (SE kit, Lonza) then resuspended with 200k HGPS cells and electroporated using the with CM-120 setting ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were passaged 5-10 times and periodically tested for contamination using MycoAlert™ Mycoplasma Detection (Lonza) kit.
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bioRxiv - Neuroscience 2020Quote: ... mouse neurons were transfected before plating with 5 μg of MAP2-GFP plasmid using a Nucleofector (Lonza) according to the manufacturer’s specifications ...
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bioRxiv - Genomics 2023Quote: ... into 96-well plates containing 5 μl modified freeze buffer (0.1% NP-40, 7.5. % DMSO, 42.5 % 2X Profreeze-CDM (Lonza) in PBS ...
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bioRxiv - Pathology 2023Quote: ... was maintained in growth media comprised of high glucose (4.5 g/L) and L-glutamine (0.6 g/L) Dulbecco’s Modified Eagle’s Media (DMEM) (#BE12-741F, Lonza), 10% foetal bovine serum (FBS ...
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bioRxiv - Microbiology 2024Quote: ... of 0.01 and incubated at 37 °C for 5-6 days in Dulbecco’s modified Eagle’s medium (DMEM; Lonza) with 2% FBS (Sigma-Aldrich) ...