Labshake search
Citations for Lonza :
1751 - 1800 of 1883 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... MCF-10A human mammary gland cells were used as the non-tumorigenic control and were cultured in MEBM basal medium supplemented with components included in the MEGMTM Mammary Epithelial Cell Growth Medium SingleQuotsTM Kit (Lonza, Hayward, CA). A549 human lung carcinoma cells were cultured in Dulbecco’s Modified Eagles Medium (DMEM ...
-
bioRxiv - Immunology 2023Quote: ... Constructs were transiently transfected into KRT17 null A431 cells using the SF Cell Line 4D-Nucleofector™ X Kit (Lonza #V4XC-2032) and Lonza 4D-nucleofector X unit “A431 cell” program ...
-
bioRxiv - Developmental Biology 2024Quote: ... 1.5 x 106 cells were transfected with 1 µg DNA (500 ng Cas9 plasmid and 500 ng linearized donor plasmid) by nucleofection (pulse code CA137) using P3 Primary Cell 4D-Nucleofector X kit (Lonza, V4XP-3024) in a 4D-Nucleofector (Lonza ...
-
bioRxiv - Immunology 2023Quote: ... T cells were then rinsed with PBS and 10 × 106 cells were resuspended in 20 µl of P4 primary cell nucleofection solution (P4 Primary Cell 4D-Nucleofector X Kit S, Lonza V4XP-4032). 20 μL of resuspended T cells were then gently mixed with 5 µL of RNP complex and incubated for 2 minutes at room temperature ...
-
bioRxiv - Developmental Biology 2022Quote: ... D-001206-14-05 5 nmol) and nucleofected into OPCs using the Basic Nucleofector Kit for Primary Mammalian Glial Cells (Lonza, VPI-1006) following manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: ... Cells were co-transfected with 1 ug of firefly Luciferase vector and 0.1 ug Renilla luciferase vector by nucleofection with Lonza Cell Line Nucleofector® Kit V (Lonza, #VVCA-1003) and the T-020 program in the Amaxa Nucleofector II device ...
-
bioRxiv - Bioengineering 2022Quote: Cell cultures were monitored for the presence of mycoplasma contamination using the MycoAlert® Mycoplasma Detection Kit (Lonza #LT07-318, Basel, Switzerland) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2022Quote: Normal bronchial epithelial cells (BEAS-2B / ATCC-CRL-9609) were cultured in collagen coated flasks using the bronchial epithelial growth medium supplemented with Lonza Bullet Kit (BEGM, Lonza CC-3170), as described by ATCC culture instructions ...
-
bioRxiv - Developmental Biology 2022Quote: ... The plasmids were co-transfected into H9 hESC cells using the Amaxa 4D nucleofector (#AAF-1003B and #AAF-1003X) and the P3 Primary Cell 4D-Nucleofector X kit (Lonza, #V4XP-3024).
-
bioRxiv - Molecular Biology 2022Quote: ... using nucleofection with the Amaxa 4D-Nucleofector X-Unit and the P3 Primary Cell 4D-Nucleofector X Kit (Lonza, V4XP-3024 KT). 2×106 cells were harvested using accutase (Sigma Aldrich ...
-
bioRxiv - Microbiology 2022Quote: ... pre-coated cell culture 6-well plate with EBM-2 endothelial media supplemented with 10% FBS and a growth factor bullet kit (Lonza, Walkersville, USA) 3 mL/well ...
-
bioRxiv - Cancer Biology 2022Quote: ... All human cell lines were authenticated by STR analysis at the JCRB Cell Bank and tested for Mycoplasma contamination using a MycoAlert Mycoplasma Detection Kit (Lonza, Basel, Switzerland).
-
bioRxiv - Cancer Biology 2023Quote: ... All cell lines used in this study tested negative for Mycoplasma contamination via MycoAlert™ PLUS Mycoplasma Detection Kit (Lonza, Basel, Switzerland).
-
bioRxiv - Cell Biology 2023Quote: PMOs were dissolved in distilled water and transfected into RD cells using the Amaxa Cell Line Nucleofector Kit L and a Nucleofector II electroporation device (Lonza, Basel, Switzerland) with program T-030 or into cells from a DMD patient without a transfection reagent.
-
bioRxiv - Molecular Biology 2023Quote: Induced pluripotent stem cells were transfected with a total of 5 μg target Cas9/gRNA plasmids with or without 1 μg repair templates using Human Stem Cell Nucleofector Kit (LONZA, Basel, Switzerland) and Nucleofector 2b device (LONZA ...
-
bioRxiv - Genetics 2024Quote: ... 3x105 Daudi cells were nucleofected with 20 picomole of sgRNA-Cas9 complex and 100 picomole of DNA donor template using program CA137 of Amaxa 4D-Nucleofector and SF cell line kit S (Lonza, V4XC-2032). The edited single-cell clones were sorted into 96-well plate by BD Aria II sorter and expanded for 4 weeks ...
-
bioRxiv - Neuroscience 2024Quote: ... following a previously described protocol.43-44 Cultures underwent testing every other month using the MycoAlert Mycoplasma Detection Kit (Lonza LT07-318) and consistently tested negative for mycoplasma throughout the study.
-
bioRxiv - Cell Biology 2024Quote: ... was transfected into 106 U2OS cells using the Amaxa Nucleofector System with the Cell Line Nucleofector Kit V (catalog no: VCA-1003, Lonza, Cologne, Germany) utilizing program X-001 according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2024Quote: ... and 1.2 μl of Alt-R® Cas9 Electroporation enhancer (100 μM, IDT) using P3 Primary Cell Nucleofector® 4D Kit and Nucleofector 4D system (Lonza). After electroporation ...
-
bioRxiv - Cancer Biology 2024Quote: ... All cell lines were authenticated and routinely tested to be mycoplasma-free using the MycoAlert Mycoplasma Detection Kit (Lonza, Cat # LT07-710) every two months ...
-
bioRxiv - Cancer Biology 2024Quote: ... Mycoplasma testing was performed on all cells when they were thawed and semi-regularly thereafter using the MycoAlert PLUS Mycoplasma Detection Kit (Lonza LT07-703). Independent experiments were performed on cells treated with siRNA and/or compounds from separate passages of each cell line ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cell lines were authenticated by short tandem repeat analysis and routinely tested for mycoplasma using the MycoAlert mycoplasma detection kit (Lonza, #LT07-710).
-
bioRxiv - Cell Biology 2024Quote: ... Cells were maintained at 37°C and 5% CO2 in 2D monolayer culture and confirmed as Mycoplasma negative (MycoAlert Mycoplasma Detection Kit, Lonza #LT07-701). Cells were maintained in DMEM medium (Corning #10-013-CV ...
-
bioRxiv - Cell Biology 2024Quote: ... the Ribonucleoprotein (RNP) complexes (9:1 sgRNA to Cas9 ratio) were assembled in Nucleofector Solution plus Supplement (Lonza Amaxa HMEC Nucleofector Kit) to a total volume of 100µL ...
-
bioRxiv - Cell Biology 2024Quote: ... In microscopy experiments we used the same media but without phenol red to reduce background fluorescence (Lonza CC-3153 phenol-red free basal media supplemented with growth factors and other components from the Lonza CC4136 kit). T98G cells were purchased from ATCC ...
-
bioRxiv - Molecular Biology 2024Quote: ... All cell lines were authenticated through ATCC STR profiling and tested regularly for mycoplasma using the Lonza MycoAlert detection kit (Lonza LT07-318).
-
bioRxiv - Microbiology 2024Quote: ... Cells were treated with mycoplasma removal agent (MP Biomedical, #3050044) and tested monthly for mycoplasma contamination using the MycoAlert Plus mycoplasma testing kit (Lonza, #LT07-318).
-
bioRxiv - Neuroscience 2024Quote: ... A total of 3 × 106 cells were centrifuged and nucleofected using the P3 Primary Cell 4D-Nu-cleofector X Kit L (Lonza, V4XP-3024), a 4D-nucleofector core unit ...
-
bioRxiv - Microbiology 2024Quote: ... with an overnight digestion prior to phenol-chloroform purification and transfection with the P3 Primary Cell 4D-Nucleofector kit (Lonza V4XP-3032) in a Amaxa 4D Nucleofector (Lonza AAF-1003X ...
-
bioRxiv - Immunology 2024Quote: ... Cell cultures were regularly analyzed for mycoplasma contamination using the MycoAlert™ Mycoplasma detection Kit following the manufacturer’s protocol (Lonza, #LT07-318) and were always tested negative for mycoplasma infections
-
bioRxiv - Genomics 2024Quote: ... 200,000 cells were nucleofected with either 500 ng pJK028A and 500 ng pJK029A or 1 μg pJKEB01 using the Lonza SF Cell Line 4D-Nucleofector X Kit S (Lonza, V4XC-2032) according to manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... All iPSC-derived cell lines were tested and negative for mycoplasma contamination using the MycoAlert Mycoplasma test kit from Lonza (#LT07-318).
-
bioRxiv - Immunology 2024Quote: ... Human primary fetal tissue was only used after it was confirmed negative for mycoplasma contamination using the MycoAlert Mycoplasma test kit from Lonza (#LT07-318).
-
bioRxiv - Microbiology 2024Quote: ... The resuspended cells together with RNP complexes were immediately transferred into the cuvette of the P2 Primary Cell Nucleofector Kit (Lonza; V4SP-2096) and electroporated using the program code ...
-
bioRxiv - Microbiology 2024Quote: ... The resuspended cells were combined with crRNP complexes and were immediately transferred into the cuvette of the P3 Primary Cell Nucleofector Kit (Lonza; V4SP-3096) and electroporated using code CM-137 on the Lonza 4D-Nucleofector ...
-
bioRxiv - Cell Biology 2024Quote: ... 8 x 105 H9 hESCs in single-cell suspension were resuspended in 100 µL electroporation buffer from the Human Stem Cell Nucleofector Solution 2 kit (Lonza, VPH-5022). Alt-R Cas9 Electroporation enhancer (1.1 µM ...
-
bioRxiv - Cancer Biology 2024Quote: ... after thaw for about 1 week and tested for mycoplasma regularly using the MycoAlertTM PLUS Mycoplasma Detection Kit (Lonza, CAT#: LT07-318). Cells were always used within 10 passages of authentication via STR analysis with CellCheck9 by IDEXX laboratories.
-
bioRxiv - Systems Biology 2024Quote: ... HiFi Cas9 Nuclease V3 protein (IDT, 1081058) were introduced into low-passage dual-reporter ESCs using the Mouse Embyronic Stem Cell Nucleofector Kit (Lonza VPH-1001). ESCs were treated with 0.025% trypsin/1% EDTA (Gibco ...
-
bioRxiv - Physiology 2024Quote: ... These cells were cultured in complete endothelial cell growth medium-2 (EGM-2) supplemented with the Bullet Kit (Lonza Biologics, Basel, Switzerland) and a 1% v/v penicillin-streptomycin antibiotics cocktail ...
-
bioRxiv - Neuroscience 2024Quote: ... RNPs were then nucleofected into 1.2-5X106 monocytes isolated the prior day using the P3 Primary Cell 4D-Nucleofector® LV Kit (Lonza #V4LP-3520) on program CM137 ...
-
bioRxiv - Molecular Biology 2024Quote: ... Cell lines were authenticated using short tandem repeat (STR) analysis and tested negative for mycoplasma using the MycoAlert mycoplasma detection kit (Lonza, Basel, Switzerland). Sigma Mission shRNAs targeting Maf1 (TRCN0000182935 ...
-
bioRxiv - Immunology 2024Quote: CYLD was knocked down by the CRISPR/Cas9 method using SG cell line 4D-nucleofector X kit (#V4XP-4024, Lonza, Basel, Switzerland). For 1x106 cells ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cell pellets were resuspended in 20uL prepared electroporation buffer (16.4uL SE or P3 + 3.6uL supplement buffer per reaction) provided by the Lonza 4D-Nucleofector X kit (SE, Fisher Scientific, #NC0828118; or P3, Lonza; #V4XP-3032.) 20uL of the cell solution was then transferred to the 5uL RNP solution and gently mixed by pipetting ...
-
bioRxiv - Genomics 2024Quote: ... Approximately 12 million cells per replicate were spun down and resuspended in nucleofection solution from the Lonza SF Cell Line 4D-Nucleofector X Kit L (Lonza, V4XC-2012) and nucleofected in 12 reactions with 5 μg pLJK07 or pLJK08 per 1 million cells according to manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2024Quote: Sequence confirmed validation plasmids were nucleofected into the PB Tet-On dCas9 K562 cell lines using SF Cell Line 96-well Nucleofector kit (Lonza, V4SC-2096). The manufacturer’s instructions were followed using 800 ng plasmid DNA per nucleofection ...
-
bioRxiv - Bioengineering 2024Quote: CHO-S cells were stably transfected by electroporation using the Amaxa Nucleofector system in combination with the Cell Line Nucleofector® Kit V (Lonza, Switzerland). 5.0 x 106 cells per cuvette were transfected with 2 µg plasmid and incubated in T75 flasks at 37 °C under 5% CO2 in 13 ml CD-CHO medium supplemented with 8 mM L-glutamine ...
-
bioRxiv - Genetics 2024Quote: ... All the cells were grown with 5% CO2 at 37°C and verified mycoplasma-free using the MycoAlert Mycoplasma Detection Kit (Lonza, LT07-218). The differentiation of WTC11 i3N iPSCs into excitatory neurons was performed using a two-step differentiation protocol ...
-
bioRxiv - Bioengineering 2024Quote: CHO cells were transiently transfected by electroporation using the Amaxa Nucleofector system in combination with the SG Cell Line 96-well Nucleofector® Kit (Lonza, Switzerland). 1.86 x 106 cells per well were transfected with 750 ng plasmid DNA or 400 ng mRNA following manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... which was introduced into CMK cells using a 4D nucleofector system and P3 primary cell 4D-Nucleotransfector X Kit L (Lonza; Basel, Switzerland). Post transfection cells were cultured in 12-well plates ...
-
bioRxiv - Cancer Biology 2024Quote: ... Cell lines were routinely tested and shown to be negative for Mycoplasma using the MycoAlert Mycoplasma Detection Kit (Lonza, Cat# LT07-118). Cells were passaged by detachment with 1 x Trypsin (Sigma-Aldrich ...