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Citations for Lonza :
1551 - 1600 of 1883 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... the PB-Tre-BMP4 and PB-blue plasmids were co-transfected into H9 using the P3 Primary Cell 96-well Nucleofector Kit (Lonza) and the 4D Nucleofector×Unit (Lonza ...
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bioRxiv - Developmental Biology 2024Quote: ... Donor and TALEN plasmids were nucleofected into WTC-hNIL stem cells using the P3 Primary Cell 4D Nucleofector X Kit (Lonza). Cells were then selected for with G418 and integration was confirmed using junction PCR (Supplemental Table 3) ...
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bioRxiv - Cell Biology 2024Quote: ... following the protocols provided by the manufacturers using the Basic Nucleofector™ Kit for Primary Mammalian Fibroblasts (VPI-1002, Lonza). After transfection ...
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bioRxiv - Cancer Biology 2021Quote: ... Mycoplasma detection was routinely performed to ensure cells were not infected with mycoplasma by using MycoAlert Detection kit (Lonza, LT07-218). Cells were maintained at 37°C in a 5% CO2 incubator ...
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bioRxiv - Cancer Biology 2021Quote: ... and transferred to the well of a 16-well Nucleocuvette™ strip (SF Cell Line 4D-NucleofectorTM X Kit S, Lonza). Cells were electroporated on a 4D-NucleofectorTM X Unit (Lonza ...
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bioRxiv - Cell Biology 2020Quote: Microspheres were tested for LPS contamination using the Limulus Amebocyte Lysate (LAL) QCL-1000™ kit (Lonza Walkersville, Inc., Olten, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were tested for mycoplasma on receipt of the cell line and quarterly thereafter using the MycoAlert Mycoplasma Detection Kit according to the manufacturer’s instructions (Lonza, Basel, Switzerland).
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bioRxiv - Immunology 2021Quote: ... All generated THP1 and iMAC clones were tested negative for mycoplasma contamination using MycoAlert Mycoplasma Detection Kit (Lonza, Cat#LT07-318). THP1 cells were obtained from ATCC ...
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bioRxiv - Developmental Biology 2020Quote: ... and nucleofected with BioID constructs using an Amaxa Nucleofector II and the Mouse Neural Stem Cell Nucleofector Kit (Lonza; VPG-1004) according the manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: CRISPR plasmid constructs (2 μg) were electroporated into 2 × 106 Raji cells using the Amaxa Cell Line Nucleofector Kit V (Lonza Bioscience) (Program M-013) ...
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bioRxiv - Neuroscience 2021Quote: ... Neuronal nucleofections were performed immediately before plating at 0 DIV by using an Amaxa Nucleofection Kit (Lonza, Basel, Switzerland, VPG-1003) according to the manufacturer’s optimized protocol (Number 101 ...
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bioRxiv - Molecular Biology 2020Quote: ... by nucleofection using the Basic Nucleofector Kit for Primary Mammalian Fibroblasts with programme A-24 according to the manufacturer’s instructions (Lonza, Cologne, Germany). Cell clones were isolated after approximately 12 days of puromycin selection (1.5 µg/mL) ...
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bioRxiv - Cell Biology 2020Quote: BMDMs (1 × 106 cells) were transfected with siRNA (250 pmol) and Amaxa Mouse Macrophage Nucleofector Kit (Lonza, catalog number VPA-1009) using a Lonza Nucleofector 2b device (Lonza ...
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bioRxiv - Microbiology 2021Quote: ... pcDNA3-JUP or empty vector were transfected into Caco-2 cells using SE Cell Line 4D-Nucleofector™ X Kit (Lonza) and an Amaxa 4D Nucleofector device from Lonza ...
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bioRxiv - Genomics 2021Quote: ... Cell lines were authenticated by STR profiling and mycoplasma testing was performed using the MycoAlert™ PLUS Mycoplasma Detection Kit (Lonza). U2OS cells were grown in DMEM supplemented with 10% tetracycline-free foetal bovine serum (Clontech) ...
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bioRxiv - Neuroscience 2020Quote: ... All cells were routinely tested for mycoplasma contamination with MycoAlert Mycoplasma Detection Kit (Lonza, Rockland, ME, USA, Cat N° LT07-318). Chemicals and cell culture reagents were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Cancer Biology 2020Quote: ... while MCF-10A cells were cultured as per ATCC recommendations in MEGM bullet kit growth media (CC-3150; Lonza, Walkersville, MD) without gentamycin-amphotericin B mix but with additional 100ng/ml cholera toxin (C8052 ...
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bioRxiv - Cell Biology 2021Quote: Cell lines were cultured according to standard aseptic mammalian tissue culture protocols in 5% CO2 at 37 °C with regular testing for mycoplasma contamination using the MycoAlert™ Mycoplasma Detection Kit (Lonza). HCT 116 ...
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Rag GTPases and Phosphatidylinositol 3-Phosphate Mediate Recruitment of the AP-5/SPG11/SPG15 ComplexbioRxiv - Cell Biology 2020Quote: ... The cell lines were routinely tested for the presence of mycoplasma contamination using MycoAlert™ Mycoplasma Detection Kit (LT07-318, Lonza), and were also regularly treated with mycoplasma removing agent (093050044 ...
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bioRxiv - Cancer Biology 2021Quote: ... in a total volume of 100μL/ reaction and electroporated using an Amaxa TM Basic Nucleofector TM Kit for Primary Mammalian Epithelial cells (Lonza, # VPI-1005) and the NucleofectorTM 2b Device (Lonza ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended with 10µL of primary cell nucleofection solution (P3 primary Cell 4D-Nucleofector X kit S (Lonza, # V4XP-3032), and mixed with RNP complexes ...
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bioRxiv - Developmental Biology 2022Quote: ... and all cell lines are routinely monitored for mycoplasma infection monthly using the MycoAlert Mycoplasma Detection Kit (Lonza, Cat#LT08-118). Stem cells were maintained as previously described (Spence et al. ...
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bioRxiv - Microbiology 2022Quote: ... Residual endotoxins in the purified phage preparations were determined by the Limulus Amebocyte Lysate (LAL) Kinetic-QCL Kit (Lonza, Basel, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... Cells were negative for mycoplasma contamination and are regularly tested using the MycoAlert PLUS Mycoplasma Detection Kit (Lonza, Rockville, MD, USA). Unless stated otherwise ...
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bioRxiv - Genomics 2022Quote: mESC were transfected with sgRNA constructs using the P3 Primary Cell 4D-Nucleofector X Kit (V4XP-3024) and the Amaxa 4D Nucleofector™ system (Lonza). We used the transfection programme CG-104 ...
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bioRxiv - Bioengineering 2022Quote: ... the basal channels of the Intestine Chips were first rinsed with endothelial cell culture medium consisting of stem cell medium supplemented with components from EGM™-2 MV Microvascular Endothelial SingleQuots Kit (CC-4147; Lonza), and then 50 μL HIMECS (450,000 cells/chip ...
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bioRxiv - Microbiology 2021Quote: ... HPMEC were cultured in endothelial cell growth basal medium 2 supplemented with an Endothelial Cell Growth Medium-2 (EGM-2™) supplemental bullet kit (Lonza). Cells were maintained at 37°C with 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... The Ala324Thr and the Glu655Asp mutations were independently recreated by transfecting the respective sgRNA template and donor dsDNA using the Basic Parasite Nucleofector™ Kit 1 (Lonza) with the U-033 program following manufacturer recommendations ...
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bioRxiv - Microbiology 2021Quote: ... DNA electroporation was conducted following the Amanxa® Cell line nucleofector protocol using the Cell Line Nucleofector® Kit R (Lonza). 2 μg plasmid DNA was used per reaction and electroporation was carried out using the I-013 program for high expression efficiency of HeLa cells ...
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bioRxiv - Molecular Biology 2020Quote: ... Transient transfection of pp150 in HFF was performed by transfecting pp150 expressing plasmid (kindly provided by Edward Mocarski) using Amaxa Nucleofection kit V (Lonza inc.). The pp71-expressing plasmid (pCGN-pp71 ...
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bioRxiv - Neuroscience 2022Quote: ... cellular ATP content was measured two-three times a week between 5-7.5 weeks post transduction using the ViaLight Plus kit (Lonza, LT07-221) or ATPlite kit (PerkinElmer ...
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bioRxiv - Immunology 2022Quote: ... were delivered into the CD4+ Human T cells by using Amaxa 4D-Nucleofector System and P2 Primary Cell 4D-Nucleofector® X Kit S (Lonza) according to manufacturer’s instructions using the pulse code EH100 ...
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bioRxiv - Immunology 2022Quote: ... were transfected with 0.1 nmol of siRNA oligonucleotides (listed in Supplementary Table 3) using a Human Monocyte Nucleofactor Kit (Lonza, VVPA-1007) and the AMAXA Nucleofector System (Lonza ...
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bioRxiv - Cell Biology 2022Quote: ... were transfected with 4 μM of each siRNA (TriFECTa® RNAi Kit, IDT) or negative control siRNA (Universal Negative Control siRNA [Nippon Gene]) by nucleofection (Lonza) using reagent V and the X-001 program ...
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bioRxiv - Biophysics 2020Quote: ... All the primary GBM cell lines were checked periodically for mycoplasma contamination using the MycoAlertTM Mycoplasma Detection Kit (Lonza, Basel, Switzerland). All Patient-derived biological samples were collected according to a protocol approved by Italian Local Ethics Committee (CE IRST IRCCS-AVR ...
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bioRxiv - Neuroscience 2020Quote: ... along with Renilla luciferase control vector using a Human Stem Cell Nucleofector Kit according to the manufacturer’s instructions (Lonza, VPH-5012). Cells were plated in a 96-well plate and terminally differentiated into neurons ...
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bioRxiv - Cell Biology 2021Quote: ... MDCKII cells were transfected by electroporation with plasmids encoding CD4-SIV Env chimeras using an Amaxa Cell Line Nucleofector™ Kit L and Amaxa Nucleofector II with settings L-05 (Lonza). Stable transfectants were selected with 400 μg/ml G418 Sulphate (Calbiochem) ...
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bioRxiv - Cell Biology 2021Quote: ... All cell lines used in this study were monitored for mycoplasma infection using the MycoAlert Mycoplasma Detection Kit (Lonza TL07-218).
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bioRxiv - Developmental Biology 2020Quote: ... or a PMO Control at 100 µM in 100 µL solution from the P3 Primary Cell 4D-Nucleofector® X Kit L (V4XP-3024, Lonza) using the CB150 program on the 4D-Nucleofector™ System (Lonza) ...
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bioRxiv - Genomics 2021Quote: ... K562 cells were grown to a density of 1×106 cells and electroporated with 5μg of plasmid DNA (Amaxa® Cell Line Nucleofector® Kit V, Lonza). Cells from each cell line were cultured for an additional 48 hours until total RNA isolation (Maxwell RSC simplyRNA kit ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and PX458-AAVS1 (3 µg) were electroporated into 1.3×106 H9 cells using the Human Stem Cell Nucleofector Kit 1 (Lonza, VPH-5012). Following electroporation the cells were seeded across 3-wells of a 6 well plate coated with Matrigel in StemFlex supplemented with RevitaCell supplement (Thermo Fisher ...
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bioRxiv - Genetics 2021Quote: ... complex and mix of 1:1 ratio WT/mutant donor single-stranded oligodeoxynucleotides carrying PAM mutation by electroporation using Mouse Embryonic Stem Cell Nucleofector™ Kit from Lonza. sgRNA and donor single-stranded oligodeoxynucleotide sequences can be found in supplementary table 5 ...
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bioRxiv - Neuroscience 2021Quote: ... 1 × 106 hiPS cells were nucleofected by Amaxa nuclefector device using Human Stem Cell Nucleofector® Kit 1 (VPH-5012, Lonza) and program B-016 with 4 μg of MyoD plasmid and 1 μg of the dual helper plasmid ...
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bioRxiv - Immunology 2021Quote: ... were washed in PBS/0.5%BSA and resuspended in 100 µl Nucleofector solution (Amaxa™ P3 Primary Cell 4D Nucleofector TM X Kit L, Lonza) with 4-6 µl of the appropriate siRNA (20-40 µM ...
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bioRxiv - Genetics 2021Quote: Human K562 cells were nucleofected with already complexed RNP using the SF Cell Line 4D Nucleofector X Kit for Amaxa 4D device (Lonza, Basel) with program FF-120 ...
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bioRxiv - Cell Biology 2021Quote: ... Media was then changed to EBM™-2 Endothelial Cell Growth Basal Medium-2 containing bullet kit growth factor supplements (Lonza), 10% fetal bovine serum ...
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bioRxiv - Cell Biology 2021Quote: ... cells were purchased from ATCC and cultured in EBM™-2 Endothelial Cell Growth Basal Medium-2 containing bullet kit growth factor supplements (Lonza), 5% fetal bovine serum ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... RBD-Fc stable clone was obtained by electroporation with 2×106 cells and 5 μg endotoxin-free plasmids using Amaxa kit V and program U24 with Amaxa Nucleofector 2B (Lonza, Switzerland). Electroporated cells were subsequently plated in 96-wells at 500 cells/well in Plating Medium containing 80% EX CELL® CHO Cloning Medium (Cat.no C6366 ...
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bioRxiv - Neuroscience 2022Quote: Dissociated rat commissural neurons were electroporated with the Amaxa 96-well Shuttle using the P3 Primary Cell 96-well Nucleofector Kit (Lonza, Switzerland). For each electroporation in one well (20 µl ...
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bioRxiv - Developmental Biology 2022Quote: ... 400,000 H9 cells for each condition were electroporated with 1 μg plasmid via a human stem cell nucleofector™ kit (VPH-5012) by Lonza AMAXA Nucleofector 2B.