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Citations for Lonza :
1601 - 1650 of 1883 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: All cell lines were obtained from ATCC and were tested for mycoplasma contamination using MycoAlert Mycoplasma Detection Kit (Lonza, Basel, Switzerland). HCT116 (RRID:CVCL_0291 ...
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bioRxiv - Cell Biology 2024Quote: ... and a control siRNA (D-001810-01-20 ON-TARGET plus Nontargeting siRNA #1) were prepared for electroporation using the Lonza SF Cell Line 4D-Nucleofector™ X Kit (V4XC-2012; Lonza) according to the manufacturer’s instruction with minor alterations ...
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bioRxiv - Neuroscience 2022Quote: The cell toxicity was determined by measuring the relative amount of adenylate kinase released from cells into the medium (ToxiLightTM Non-Destructive Cytotoxicity BioAssay Kit, LONZA, Swittzerland). Medium from cell culture was mixed with adenylate kinase substrate adenosine diphosphate ...
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bioRxiv - Molecular Biology 2022Quote: ... collected by centrifugation at 500xg for 5 min and resuspended in SF solution (SF cell line 4D-Nucleofector™ X Kit, Lonza). After addition of the RNP ...
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bioRxiv - Cancer Biology 2022Quote: ... Pelleted organoids were resuspended in 20uL of nucleofection mix and transferred to electroporation chamber (Lonza kit, #V4XP-3032, 96-well format) for electroporation using Lonza X Unit Nucleofector under the [ES ...
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bioRxiv - Bioengineering 2024Quote: ... HepG2 and K562 cells (2×105 cells/condition) were electroporated using the SF Cell Line 96-well Nucleofector™ Kit (Lonza) and the CM-130 ...
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bioRxiv - Bioengineering 2024Quote: ... The day of the electroporation cells were collected and washed twice with phosphate buffered saline (PBS) and resuspended in nucleofection buffer (SF/SE Cell Line 96-well Nucleofector™ Kit; Lonza) containing 2 μg of PEn mRNA and 100 pmol of synthetic springRNA or spaceRNA ...
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bioRxiv - Neuroscience 2024Quote: ... digested for 3 min at 37 °C in Accutase and then resuspended in 60 μL nucleofection buffer from the Human Stem Cell Nucleofector™ Kit 2 (Lonza). The suspension was combined 2 μM Electroporation Enhancer (IDTDNA ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were negative for mycoplasma contamination (routinely tested for the presence of mycoplasma contamination using MycoAlert™ Mycoplasma Detection Kit (LT07-318) from Lonza Bioscience Blackley ...
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bioRxiv - Immunology 2024Quote: ... were transfected with 0.1 nmol of siRNA oligonucleotides (listed in Extended Data Table 3) using a Human Monocyte Nucleofactor Kit (Lonza, VVPA-1007) and the AMAXA Nucleofector System (Lonza ...
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bioRxiv - Molecular Biology 2024Quote: ... All cell lines were confirmed to be mycoplasma negative every 3 weeks (MycoAlert™ Mycoplasma Detection Kit, Lonza, Bend, OR, USA). Fifteen micrograms of total whole cell lysates (WCL ...
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bioRxiv - Neuroscience 2023Quote: ... Approximately 2 million cells were nucleofected in a large cuvette per reaction using Lonza’s P3 primary cell 4D-nucleofector kit (V4XP-3024, Lonza, Basel, Switzerland). The cells were allowed to recover from the transfection for 3 days while inducing MNX+ motor neuronal cell fate ...
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bioRxiv - Cell Biology 2023Quote: ... These guide constructs were then electroporated into mESCs using the Lonza Nucleofector 2b Device and Cell Nucleofector Kit (Lonza #VAPH-1001). mESCs were cultured in feeder-free 2i media [DMEM/F-12 (Gibco #11320033 ...
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bioRxiv - Cancer Biology 2023Quote: ... All cell lines (and their derivatives) were confirmed to be mycoplasma-free using the MycoAlert mycoplasma detection kit (Lonza LT07- 218) at the beginning and upon completion of all experiments.
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bioRxiv - Cancer Biology 2023Quote: ... We periodically tested all the cell lines for mycoplasma contamination using the MycoAlert Mycoplasma Detection Kit (Lonza, cat. no. LT07-118). Culturing conditions were as follows ...
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bioRxiv - Immunology 2023Quote: ... Electroporation was performed with sgRNA/Cas9 RNP complexes using Alt-R Sp Cas9 Nuclease V3 (IDT) and using the 4D-Nucleofector with P3 Primary Cell 4D Nucleofector X Kit S (Lonza Bioscience).
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bioRxiv - Neuroscience 2022Quote: Dissociated rat commissural neurons were electroporated with the Amaxa 96-well Shuttle using the P3 Primary Cell 96-well Nucleofector Kit (Lonza, Switzerland). For each electroporation in one well (20 µl ...
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bioRxiv - Developmental Biology 2022Quote: ... 400,000 H9 cells for each condition were electroporated with 1 μg plasmid via a human stem cell nucleofector™ kit (VPH-5012) by Lonza AMAXA Nucleofector 2B.
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bioRxiv - Cancer Biology 2022Quote: ... Identity of cell lines was ensured by UNC’s Tissue Culture Facility with genetic signature analyses and examination of mycoplasma contamination performed using commercial detection kits (Lonza, #LT27-286).
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bioRxiv - Cell Biology 2022Quote: ... All cell lines were found to be free of mycoplasma using the MycoAlert® PLUS Mycoplasma Detection Kit (Lonza #LT07-703).
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bioRxiv - Immunology 2022Quote: ... In vitro transcribed mRNAs encoding the TCR α- and β-chains were mixed at a 1:1 ratio and electroporated in activated T cells on a Lonza 4D-NucleofectorTM device using the P3 Primary Cell 4D-Nucleofector™ X Kit (Lonza) according to the recommendations of the manufacturer ...
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bioRxiv - Immunology 2022Quote: ... Cells were maintained by twice-weekly passage and checked for mycoplasma contamination regularly using a MycoAlert mycoplasma detection kit (Lonza, UK). IAV strain A/California/04/2009 (H1N1 ...
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bioRxiv - Physiology 2022Quote: ... 1 million cells were washed with PBS and electroporated with 5-10 μg of appropriate plasmid using Amaxa cell nucleofector kit T (Lonza laboratories). Cells were allowed to recover for 48 h ...
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bioRxiv - Microbiology 2022Quote: ... NuLi-1 cells routinely tested negative for mycoplasma infection using MycoAlert Mycoplasma Detection Kit (LT07-418, Lonza Group AG, Basel, Switzerland) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... Nucleofection was performed using the 4-D nucleofector system (AMAXA) and the P3 Primary Cell 4D-Nucleofector Kit (Lonza, V4XP-3024) following manufactures’ instructions (program CB-150) ...
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Optimisation of immunocytochemistry methodology for the detection of endogenous eIF2B localised focibioRxiv - Cell Biology 2022Quote: ... Cells were maintained at 37ºC under 5% CO2 in a humidified atmosphere and routinely tested for contamination with MycoAlter™ Mycoplasma Detection Kit (Lonza, UK). Cells were validated through lineage-specific markers - anti-GFAP antibody ...
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bioRxiv - Cell Biology 2023Quote: ... All cell lines were maintained at 37°C under 5% CO2 and were routinely tested for contamination with MycoAlert™ Mycoplasma Detection Kit (Lonza).
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bioRxiv - Cancer Biology 2023Quote: ... All organoid lines were frequently tested and resulted in all cases negative in the MycoAlert mycoplasma detection kit (Lonza, LT07-318). For epithelial differentiation ...
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bioRxiv - Cancer Biology 2023Quote: ... were assembled in vitro following the manufacturer’s protocol and delivered into OCI-Ly7 cells by electroporation using SF Cell Line 96-well Nucleofector Kit (Lonza; V4SC-2096). ssODN for CREBBP R1446C mutagenesis was added along with RNP complex during electroporation as needed ...
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bioRxiv - Cell Biology 2023Quote: ... All cells were maintained at 37°C in a 5% CO2 humidified atmosphere and were regularly screened for the presence of mycoplasma using the MycoAlert Mycoplasma Detection Kit (Lonza, Switzerland).
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bioRxiv - Synthetic Biology 2024Quote: ... Then the supernatant was removed and cells were resuspended in 100 µl room-temperature Nucleofector® Kit V (Lonza, VCA-1003) solution ...
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bioRxiv - Cell Biology 2024Quote: ... All human iPSC lines screened negative for mycoplasma contamination every other month using a MycoAlert PLUS detection kit (Lonza, LT07-710).
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bioRxiv - Cell Biology 2024Quote: ... All mouse iPSC lines screened negative for mycoplasma contamination every other month using a MycoAlert PLUS detection kit (Lonza, LT07-710).
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bioRxiv - Bioengineering 2024Quote: ... and recovered for at least 24 hours in Hepatocyte Culture MediaTM (HCM) including the complete HCM bullet kit (Lonza, #CC-3198) according to the supplier’s protocol ...
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bioRxiv - Genomics 2024Quote: ... Cell lines in culture were screened biweekly for mycoplasma contamination using the MycoAlert PLUS Mycoplasma Detection Kit (Lonza, 801 #LT07-703).
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bioRxiv - Cancer Biology 2024Quote: ... were purchased from Sigma and transfected into MCF-7 cells at a final concentration of 30 nM by electroporation using the SE Cell Line 4D-Nucleofector X kit (Lonza; EEID:SCR_023155). The cells were used for various assays 48 hours after siRNA transfection.
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bioRxiv - Bioengineering 2024Quote: ... All stem cell and organoid lines were routinely monitored for mycoplasma using the MycoAlert Mycoplasma Detection Kit (Lonza Cat#LT07-318).
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bioRxiv - Cancer Biology 2024Quote: ... Primary CD3+ T cells (1×106 cells/transfection) were transfected using a Lonza 4D nucleofector™ (Pulse code EH115) with a P3 nucleofection kit (Lonza) following manufacturer’s recommendations with the indicated DNA concentrations ...
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bioRxiv - Cancer Biology 2024Quote: ... K562 and NALM6 cells (2×105 cells/transfection) were transfected using a Lonza 4D nucleofector™ with a SF nucleofection kit (Lonza) following manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: ... The cells in the buffer were then transferred to a 16-well Nucleocuvette™ Strip (Lonza, 4D-Nucleofector™ X Kit) for nucleofection using the pulse code EH-115 ...
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bioRxiv - Cell Biology 2024Quote: ... 2 × 105 to 1 x 106 HSPCs per condition were electroporated two days after thawing with 3.0 μg of the ABE-encoding mRNA and 3.2 μg of the synthetic sgRNA using the P3 primary cell 4D-Nucleofector X Kit S (Lonza, Basel, Switzerland) and the CA-137 program (Nucleofector 4D) ...
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bioRxiv - Neuroscience 2024Quote: ... Transient transfections of primary neurons with siRNA were conducted by electroporation using Lonza Amaxa Rat Neuron Neucleofector kit (Lonza, VPG-1003) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Naive DOCK2-GFP OT-I T cells were resuspended in P4 Primary Cell 4D-Nucleofector™ X Kit S (V4XP-4032, Lonza) buffer solution at a cell concentration of 5 x 106 cells in 20 µL ...
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bioRxiv - Immunology 2024Quote: ... all from Dharmacon) complexed with TrueCut Cas9 Protein v2 using the SE Cell Line kit and 4D-Nucleofector X Unit (Lonza, RRID:SCR_023155) according to manufacturer’s protocol.
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bioRxiv - Cell Biology 2024Quote: ... brucei 13-90 cells were electroporated with 10 µg of linearised plasmid DNA using the Amaxa Basic Parasite Nucleofector Kit 1 and Nucleofector II device (program X-001) (Lonza, Switzerland).
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bioRxiv - Biochemistry 2024Quote: ... 5.0 × 106 OSCs were mixed with 200 pmol of siRNAs in 20 μL of Solution SF of the Cell Line Nucleofector Kit SF (Lonza Bioscience) and electroporation was performed using a Nucleofector 96-well Shuttle device (Lonza Bioscience) ...
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bioRxiv - Neuroscience 2024Quote: ... and pCAG-Puro-Hs-HARE5 (2µg) were electroporated into 1×106 C3649 cells using the P3 Primary Cell 4D-Nucleofector kit (Lonza, V4XP-3024). The selection and genotyping process was the same as above ...
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bioRxiv - Neuroscience 2024Quote: ... and pCAG-Puro-Pt-HARE5 (2µg) were electroporated into 1×106 H9 cells using the P3 Primary Cell 4D-Nucleofector kit (Lonza, V4XP-3024). Following each electroporation ...
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bioRxiv - Neuroscience 2024Quote: All lines tested negative for mycoplasma contamination in this study were checked by the MycoAlert Mycoplasma Detection Kit (Lonza, LT07-518). One human ESC line WA09 (H9) ...
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bioRxiv - Synthetic Biology 2024Quote: ... K562 cells were electroporated with 800ng of Bxb1 pDNAusing the SF cell line 96-well Nucleofector kit (Lonza, Catalog#V4SC-2960), using manufacturer’s protocol ...