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Citations for Lonza :
1151 - 1200 of 4565 citations for Primary Human Retinal Microvascular Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... EGM-2 was prepared by combining components of an EGM-2 “bullet kit” (Cat no. CC-4176) and endothelial basal medium (EBM, Lonza, Walkersville ...
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bioRxiv - Microbiology 2022Quote: ... Both HPMEC and HUVEC cell lines were propagated (passages 5–10) and maintained in endothelial growth medium 2 (EGM-2) using the EGM-2 bullet kit from Lonza following the manufacturer’s specifications and grown in a CO2 incubator at 37°C with 5% CO2.
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bioRxiv - Bioengineering 2023Quote: ... washed three times in PBS and cultured on collagen-coated 6-well plates in endothelial growth medium (EGM-2, Lonza) composed of endothelial basal medium supplemented with 2% fetal bovine serum ...
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bioRxiv - Genomics 2022Quote: ... cells (from two donors) were grown to 80% to 90% confluence in endothelial basal medium 2-MV with supplements (EBM; Lonza) and 5% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2022Quote: ... The Cas9 protein and sgRNA were electroporated into the T cells by using Amaxa 4D-Nucleofector System and P4 Primary Cell 4D-Nucleofector® X Kit S (Lonza, V4XP-4032) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell pellets of 1E6 cells were resuspended with 20ul supplemented nucleofector solution of the P3 Primary Cell 4D-nucleofector® X Kit S (Lonza, V4XP-3032) and 2ug plasmid (1ug/ul) ...
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bioRxiv - Bioengineering 2023Quote: ... PiggyBac transposon constructs and RNA encoding transposase were introduced into T cells by electroporation using a Lonza 4D-Nucleovector instrument and the P3 Primary Cell Kit (Lonza, Cat. # V4XP-3032). Electroporated T cells were immediately seeded into 24-well G-Rex plates (Wilson Wolf ...
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bioRxiv - Cancer Biology 2023Quote: Electroporation of multiplex CRISPR-Cas9 pX330 plasmids into normal bronchial epithelial cells was achieved using the P3 Primary Cell 4D-Nucleofector® X Kit L (Lonza, V4XP-3024) and 4D-Nucleofector™ X Unit (Lonza) ...
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bioRxiv - Developmental Biology 2024Quote: ... and 3.0 × 106 cells were electroporated using the P3 Primary Cell 4D-Nucleofector L kit and 4D-Nucleofector system (Lonza Group Ltd., Basel, Switzerland), following the manufacturer’s protocol (1320v ...
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bioRxiv - Cancer Biology 2021Quote: ... Immortalized human bronchial epithelial BEAS-2B cells were purchased from ATCC and cultured in BEGM bronchial epithelial cell growth medium (Lonza #CC-4175). All the cells were grown at 37°C with 5% CO2.
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bioRxiv - Evolutionary Biology 2020Quote: ... plasmids pSpCas9n(BB)-ZEB2-Guide-A &-B (0.5 µg of each plasmid) were electroporated into 1×106 H9 cells using the Human Stem Cell Nucleofector Kit 1 (Lonza, VPH-5012). Following electroporation ...
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bioRxiv - Cell Biology 2024Quote: ... 8 x 105 H9 hESCs in single-cell suspension were resuspended in 100 µL electroporation buffer from the Human Stem Cell Nucleofector Solution 2 kit (Lonza, VPH-5022). Alt-R Cas9 Electroporation enhancer (1.1 µM ...
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bioRxiv - Bioengineering 2020Quote: Human MSCs (hMSCs) were isolated from fresh unprocessed bone marrow from human donors (Lonza) as previously described 63 ...
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bioRxiv - Genetics 2021Quote: ... RNPs containing recombinant CRISPR/Cas9 and sgRNA were transfected to human cell lines using a Nucleofector (Lonza). Stable cell lines were generated by selection of hygrogmycin resistance ...
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bioRxiv - Microbiology 2019Quote: ... brucei using the Human T-cell nucleofection® kit and an Amaxa® Nucleofector™ (Lonza AG) set to program X-001 ...
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bioRxiv - Developmental Biology 2019Quote: ... The human Atoh8 expression plasmid [30] was transfected by electropulsing using the Cell Line Nucleofector System (Lonza) following standard procedures provided by the manufacturers.
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bioRxiv - Biophysics 2020Quote: Human mesenchymal stem cells (PT-2501) used for proof-of-principle dSTORM imaging were purchased from Lonza Group ...
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bioRxiv - Cell Biology 2021Quote: HEK293 (human embryonic kidney 293) cells were grown in Dulbeccos Modified Eagles Medium (DMEM, Lonza, Basel, Switzerland) supplemented with 10% (v/v ...
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bioRxiv - Microbiology 2021Quote: ... whereas human monocyte cell line U937 (ATCC CRL-1593.2) was maintained in complete RPMI 1640 media (Lonza) with 100 µM β-mercaptoethanol and differentiated to macrophages using 20 ng/ml PMA for 24 hours prior to infection ...
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bioRxiv - Microbiology 2021Quote: Normal human bronchial epithelial (NHBE) cells (donor 41219) were purchased from Lonza (Walkersville, MD Cat# CC-2540) and maintained in Bronchial Epithelial Cell Growth Medium (BEGM ...
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bioRxiv - Biophysics 2023Quote: the first cell lineage (named ASMC_1 onwards) is a commercial immortalized human ASMCs lineage purchased from Lonza and delivered at passage 3 ...
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bioRxiv - Cancer Biology 2023Quote: Human breast adipose tissue was incubated in mammary epithelial cell growth base medium (MEBM) (Lonza #cc-3151) supplemented with 0.5% bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2021Quote: MLIV patient fibroblasts were grown for 2-3 days until 80-90% confluent at 37°C and prepared for electroporation with approximately 50nM of ON-TARGETplus siRNA SMARTpool reagents from Horizon Cell Solutions (Dharmacon) according to manufacturer instructions using the Amaxa P2 Primary Cell 4D-Nucleofector X Kit (Lonza, Cat. No. V4XP-2032) with nucleocuvette strips in a Lonza 4D-Nucleofector X Unit ...
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bioRxiv - Neuroscience 2021Quote: ... counted and 2×106 cells were transfected using the Basic Nucleofector kit for primary neurons (VAPI-1003, Lonza) and the D-33 programme on the Amaxa Nucleofector II B device (Amaxa Biosystems) ...
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bioRxiv - Microbiology 2020Quote: Electroporation was performed using the Amaxa P3 Primary Cell 96-well Nucleofector kit and 4D Nucleofector system (Lonza). Recombinant S ...
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bioRxiv - Neuroscience 2021Quote: ... freshly isolated cortical neurons were transfected using the P3 Primary Cell 4D-Nucleofector Kit (Lonza, LZ-V4XP-3024) and the program DC-104 of the 4D-Nucleofector device (Lonza) ...
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bioRxiv - Cell Biology 2021Quote: ... RNP was delivered by electroporation using a Lonza 4D-nucleofector and P3 Primary Cell Kit (Lonza, V4XP-3012). Cells were transferred to fresh StemSpan media and corresponding (AK2 or AAVS1 ...
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bioRxiv - Immunology 2022Quote: ... 1 x 10^6 purified naive T-cells were resuspended in 15µL buffer P3 + 5µL Supplement 1 (P3 Primary Cell 4D-NucleofectorTM X Kit S; Lonza) and mixed with 2.7µL RNP in the 16-well strips provided in the kit ...
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bioRxiv - Immunology 2019Quote: ... Lifeact-GFP was transiently transfected into primary T cell blasts using the Amaxa 96-well shuttle system (Lonza) and the recommended protocol for activated human T cells.
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bioRxiv - Neuroscience 2024Quote: ... we used the 4D-Nucleofector system and Amaxa P3 primary Cell 4D-Nucleofector X Kit S from Lonza. The electroporation buffer used was P3 primary cell Nucleofector Solution with Supplement 1 (Lonza) ...
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bioRxiv - Immunology 2024Quote: ... 16.4µl P3 and 3.6µl Supplement Reaction solutions from P3 Primary Cell 4D X Kit S (Lonza V4XP-3032) were added ...
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bioRxiv - Neuroscience 2023Quote: ... CRISPR reagents were transfected into iPSCs using the P3 Primary Cell 4D Nucleofector™ X Kit S (Lonza), as previously described (Deneault et al. ...
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bioRxiv - Neuroscience 2021Quote: ... 1 × 106 cells were resuspended in 100 μl of the nucleofection hESC solution 1 (Human Stem Cell Nucleofector® Kit 1/ Lonza #VPH-5012) where 5 μg of plasmids were added previously ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Normal human lung fibroblasts (Lonza) and Primary Human IPF Lung Parenchymal Fibroblasts (Donor2 ...
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bioRxiv - Cell Biology 2020Quote: ... Normal human astrocytes (NHA)(Lonza) were thawed at P5 and cultured for 5-10 days prior to cell seeding ...
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bioRxiv - Bioengineering 2024Quote: ... and recombinant human insulin 0.5%) (Lonza). Human cervical cancer cell line SiHa from ATCC (ATCC® HTB-35™ ...
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bioRxiv - Biophysics 2024Quote: Human umbilical vein ECs (Lonza) were cultured using standard protocols in Endothelial Growth Medium (EGM2 ...
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bioRxiv - Microbiology 2024Quote: ... normal human bronchial epithelial (Lonza), and porcine primary nasal epithelial (24 ...
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bioRxiv - Bioengineering 2023Quote: Human bone marrow aspirates (Lonza) were purchased to isolate primary hMSCs ...
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bioRxiv - Microbiology 2023Quote: Human Intestinal Myofibroblasts (HIMF) (Lonza) were trypsinized ...
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bioRxiv - Cell Biology 2021Quote: ... and Subset Four (podoplanin+CD36+) were sorted into 5mL FACS tubes containing endothelial basal media (EGM-2 medium without supplements added, Lonza, USA) supplemented with 10% FBS at 4°C degrees ...
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bioRxiv - Bioengineering 2020Quote: Primary hMSCs (Lonza, Inc., Allendale, NJ) were expanded in Dulbecco’s Modified Eagle’s medium (DMEM ...
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bioRxiv - Molecular Biology 2019Quote: Primary HBECs were sourced from Lonza with permission for use in research applications by informed consent ...
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bioRxiv - Bioengineering 2021Quote: Primary hMSCs (Lonza, Inc., Allendale, NJ) were expanded in Dulbecco’s Modified Eagle’s medium (DMEM ...
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bioRxiv - Cell Biology 2022Quote: Rat Primary hepatocytes (Wister, Lonza, RICP01) were cultured as instructed ...
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bioRxiv - Bioengineering 2023Quote: Primary NHLF were purchased from Lonza and subcultured for up to six passages in manufacturer supplied growth medium (FGM-2 BulletKit ...
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bioRxiv - Microbiology 2020Quote: ... The previously described human proximal tubular epithelial cell line HPT-1b[26] was maintained in renal epithelial cell growth media (Lonza, cat. no. CC-4127). To generate the HPT-1b-mCherry cell line ...
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bioRxiv - Neuroscience 2023Quote: ... 4−106 cells were pelleted at 300xg and then resuspended in 100 μL prewarmed nucleofector solution (Human Stem Cell Kit 1, Lonza, catalog no. VPH-5012); 4 μg of NGN2 plasmid and 1.5 μg each of 1R and 1L plasmids were added directly to the resuspended cells ...
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bioRxiv - Cell Biology 2022Quote: Human embryonic kidney 293 T-antigen (HEK293T) cells (ATCC) were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Lonza) and supplemented with 10% fetal bovine serum (Thermo Fischer Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... APRE-19 and Human embryonic kidney (HEK) 293T cells (ATCC) were maintained in Dulbecco’s Modified Eagle’s Medium (Lonza) supplemented with 10% fetal calf serum (Serana ...