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Citations for Mabtech :
401 - 450 of 585 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: (ALP) (Mabtech) following manufactures directions ...
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bioRxiv - Immunology 2021Quote: ... Reactivity was assessed by specific IFN-γ release by ELISpot assay by coating ELISpot plates (MAHAS4510) with IFN-γ capture antibody (MABTECH Cat# 3420-3-250, RRID:AB_907283) followed by application of IFN-γ-detection antibody (MABTECH Cat# 3420-6-250 ...
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bioRxiv - Immunology 2021Quote: ... followed by application of IFN-γ-detection antibody (MABTECH Cat# 3420-6-250, RRID:AB_907273) and visualization by Streptavidin horseradish peroxidase (HRP ...
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bioRxiv - Immunology 2021Quote: ... plates were incubated with a fluorescence enhancer (MABTECH). Cytokine producing cells were counted using an automated fluorescence plate reader (AID-iSpot reader ...
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bioRxiv - Immunology 2021Quote: ... Immobilon-FL PVDF 96-well plates (Mabtech) were coated overnight at 4°C with mouse anti-human IFN-γ (clone 1-D1K) ...
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bioRxiv - Immunology 2021Quote: To assess memory T cell responses to SARS-CoV-2 in convalescent COVID-19 patient samples we utilized Human IFN-γ/IL-2/TNF FluoroSpot kit (Mabtech), which allows for the detection of T cells producing IFN-γ ...
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bioRxiv - Immunology 2021Quote: ... IL-2 (Cy3) and/or TNF (Cy5) were detected with IRIS FluoroSpot reader and counted with Apex software (Mabtech). Numbers of responding cells in negative controls were subtracted from stimulated samples to account for background responses ...
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bioRxiv - Immunology 2021Quote: ... IFN-α level in the culture supernatant was measured using ELISA (Mabtech, Stockholm, Sweden).
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bioRxiv - Immunology 2021Quote: ELISpot plates (Mabtech) were pre-coated with recombinant SARS-CoV-2 trimeric spike (1μg/ml) ...
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bioRxiv - Immunology 2021Quote: ... followed by streptavidin-HRP (3310-9-1000, Mabtech AB). The plates were developed with 1-Step Ultra TMB-ELISA Substrate Solution (34028 ...
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bioRxiv - Immunology 2021Quote: Antigen-specific cellular responses were measured by IFNγ Fluorospot assay with all antibodies and reagents from Mabtech (Nacka Strand, Sweden). Plates were coated overnight at 4°C with a mouse anti-human IFNγ (clone 1-D1K ...
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bioRxiv - Immunology 2021Quote: ... and visualization by Streptavidin horseradish peroxidase (HRP) (MABTECH Cat# 3310-9-1000).
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bioRxiv - Immunology 2021Quote: ... Cell culture supernatants were analysed with the bovine IFNγ ELISA development kit (MABTECH, Cat no. 3119-1H-20) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... IFNγ-BAM and IL-2-biotin (MABTECH). This was followed by incubation with fluorophore labelled secondary antibodies ...
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bioRxiv - Immunology 2021Quote: ... were coated with monoclonal antibodies to bovine IFNγ and IL-2 (MABTECH). Duplicate wells were seeded with 1×105 cells per well of cryopreserved bovine PBMCs (rested overnight before use ...
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bioRxiv - Immunology 2021Quote: ... anti-BAM-490 and streptavidin-550 (MABTECH). Finally ...
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Efficacy of recombinant NP-M1 and NP-M1-CRT DNA vaccines against Influenza A viruses in mice C57/BL6bioRxiv - Molecular Biology 2021Quote: ... The number of NP-specific IFN□ secreting cells in mouse was counted using commercial ELISPOT assay kits (MABTECH AB, Sweden). This procedure was conducted according to the instruction manual ...
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bioRxiv - Immunology 2021Quote: ... (MabTech, Human IFNγ ELISpot Plus). Cells were then washed off ...
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bioRxiv - Immunology 2021Quote: ... ALP-conjugated streptavidin (Mabtech, Sweden, 3310-10) was added at 1:1000 ...
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bioRxiv - Immunology 2021Quote: ... Spots were counted by computer-assisted image analysis (Mabtech IRIS, Mabtech). The responses were considered positive if they met all three criteria (I ...
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bioRxiv - Immunology 2021Quote: ... Spots were counted by computer-assisted image analysis (Mabtech IRIS, Mabtech). The responses were considered positive if they met all three criteria (I ...
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bioRxiv - Immunology 2021Quote: ... and MTB300 (1µg/ml) peptide pools were measured by IFN-γ Fluorospot assay (Mabtech), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Plasma IFNα concentrations were measured with the Human IFN-α ELISAPROkit (Mabtech), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Fluorescent spots indicating a single mASC and relative volume of antibody produced were detected with an IRIS FluoroSpot Reader and counted with Apex software (Mabtech). Positivity thresholds were set for both S1 and N mASCs based on prepandemic control samples’ average S1 or N plus three standard deviations.
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bioRxiv - Immunology 2021Quote: ... cryopreserved PBMCs from study subjects were stimulated for 5 days with polyclonal B cell stimulation (1μg/mL R848 and 10 ng/mL rIL-2, Mabtech) in R10 media (RPMI 1640 with 10% FCS ...
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bioRxiv - Immunology 2021Quote: Three peptide pools were used in memory T cell FluoroSpot assay: (i) SNMO defined peptide pool (Mabtech) consisting of 47 peptides (8 – 18 amino acid long ...
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bioRxiv - Immunology 2021Quote: ... were measured using a multicolor B cell FluoroSpot kit with modifications (Mabtech). Briefly ...
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bioRxiv - Immunology 2021Quote: ... plates were treated with a Fluorescence enhancer (Mabtech) to optimize detection and then air-dried ...
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bioRxiv - Immunology 2021Quote: ... FluoroSpot assay was performed using mouse IFN-γ FluoroSpotPLUSkit according to the manufacturer’s instructions (Mabtech, Inc., Cincinnati, OH). Briefly ...
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bioRxiv - Immunology 2021Quote: 96-well FluoroSpot plates were coated with anti-cytokine antibodies for IFNγ and IL-5 (mAbs 1-D1K and TRFK5, respectively; Mabtech, Stockholm, Sweden) at a concentration of 10μg/mL ...
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bioRxiv - Immunology 2021Quote: ... cells were discarded and plates were washed before the addition of cytokine antibodies (mAbs 7-B6-1-BAM and 5A10-WASP; Mabtech, Stockholm, Sweden). After a 2-hour incubation ...
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bioRxiv - Immunology 2021Quote: ... washed with sterile PBS and coated overnight at 4 °C with anti–IFN-γ antibody (10 µg/ml in PBS; clone 1-D1K; Mabtech, Nacka Strand, Sweden). Prior to use ...
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bioRxiv - Immunology 2021Quote: ... followed by two washes with PBS and then incubated for 2 hr at RT with biotinylated anti-IFN-γ (1 μg/mL; clone mAb-7B6-1; Mabtech). After six further washes ...
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bioRxiv - Immunology 2021Quote: ... Plates were then washed six times and developed using VECTASTAIN® Elite ABC-HRP according to the manufacturer’s instructions (Mabtech). All assays were performed in duplicate ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated with alkaline phosphatase-conjugated streptavidin (Mabtech) at 1:1000 dilution for 1 hr at RT ...
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bioRxiv - Immunology 2021Quote: ... followed by streptavidin – HRP (Mabtech). TMB substrate (KPL ...
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bioRxiv - Immunology 2021Quote: ... were coated with IFN-γ capture antibody at a concentration of 15 µg/mL (Mabtech). The following day ...
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bioRxiv - Immunology 2021Quote: ELISpot assays were performed with mouse IFN-γ and IL-4 ELISpot PLUS kits according to the manufacturer’s instructions (3321-4HPT-10 and 3311-4HPW-10, Mabtech, Herndon, VA, USA). A total of 2,5 × 105 or 4 x 105 splenocytes from female mice were seeded per well for the IFN-γ and IL-4 tests respectively and ex vivo stimulated either with the 1:1 mix of the RBD overlapping peptides from the B.1.1.7 (alpha variant ...
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bioRxiv - Immunology 2021Quote: ... or the Human IFN-a pan ELISA kit (Mabtech 3425-1M-6 ...
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bioRxiv - Immunology 2021Quote: Monkey IFNγ ELISpotPRO plates (MABTECH, 3421M-2AST-2) were washed with PBS and blocked overnight at 4°C in RPMI10+ media ...
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bioRxiv - Genomics 2020Quote: ... Cells were stimulated with soluble anti-CD3 (5 μg/mL; MabTech, Cincinnati, OH) and anti-CD28 antibodies (10ug/mL ...
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bioRxiv - Genomics 2020Quote: ... and anti-CD28 antibodies (10ug/mL; MabTech, Cincinnati, OH) at 37 Celsius ...
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bioRxiv - Immunology 2020Quote: ... Cells were then discarded and plates were further incubated with biotinylated IFNγ antibody (Mab 7-B6-1; Mabtech, Stockholm, Sweden) and incubated for 2hr at 37 C° ...
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bioRxiv - Immunology 2020Quote: ... 5% CO2 at a concentration of 1×105 cells/mL media on plates previously coated with anti-human IFNγ (Mab 1-D1K; Mabtech, Stockholm, Sweden). Cells were then discarded and plates were further incubated with biotinylated IFNγ antibody (Mab 7-B6-1 ...
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bioRxiv - Immunology 2020Quote: ELISpot membranes (MabTech, Macleod, Australia) were coated with anti-IgM antibodies ...
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bioRxiv - Immunology 2020Quote: ... cells were discarded and secreted IgM was visualized (MabTech, Macleod). The developed spots were quantified by an ELISpot reader (ELISpot Reader System ...
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bioRxiv - Microbiology 2020Quote: Murine IFN-γ and IL-5 ELISPOT assays were performed following manufacturer’s procedures for mouse IFN-γ and IL-5 ELISPOT kit (Mabtech, Cincinnati, OH). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... Baboon IFN-γ and IL-4 assays were carried out using NHP IFN-γ and Human IL-4 assay kit from Mabtech using PBMC collected at day 7 following the second immunization (day 28).
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bioRxiv - Immunology 2020Quote: ... ALP-conjugated Streptavidin (Mabtech, Sweden, 3310-10) was added at 1:1000 ...
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bioRxiv - Immunology 2020Quote: ... α-perforin–FITC (clone pf344) from Mabtech AB ...