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Citations for Mabtech :
251 - 300 of 585 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... 100 µL/well of biotinylated mAb anti-human IgG (Mabtech) diluted to 0.5 µg/mL ...
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bioRxiv - Immunology 2022Quote: ... 50 µL/well of Fluorescent enhancer II (Mabtech) were added followed by incubation for 10 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: ... the cells were washed and plated onto PVDF ELISpot filter plates coated with anti-mouse IgG or IgA capture antibody (Mabtech, Cat# BASIC 3825-2H and BASIC 3835-2H). The plates were incubated at 37°C for 24 hours ...
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bioRxiv - Immunology 2022Quote: ... PBMC were thawed and cultured at 37°C and 5% CO2 in the presence of 1 μg/mL R848 and 10 ng/mL of recombinant interleukin (IL) 2 (both from Mabtech).
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bioRxiv - Immunology 2022Quote: ... 100 µL of either goat anti-mouse IgG (for 6E2 hybridoma cells) or mouse anti-human IgG (for EBV-immortalized human B cells) specific for the Fc region (Mabtech) diluted in sterile PBS at 15 µg/mL were added to wells followed by incubation at 4°C for 24 hours ...
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bioRxiv - Immunology 2022Quote: ... kits with pre-coated plates for enumeration of cells secreting IFN-γ and IL-17A were purchased from Mabtech (Cat. No. FSP-414443-2). Spots were enumerated on an AID iSpot EliSpot/ FluoroSpot Reader.
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bioRxiv - Immunology 2022Quote: IFN-γ ELISPOT assay was performed using a Mouse IFN-γ ELISpot antibody pair (Mabtech, Sweden). Splenocytes were isolated in RPMI culture medium (Gibco ...
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bioRxiv - Immunology 2022Quote: ... A T cell antigen control pool (CEF, Mabtech AB, 3616-1) at a final concentration of 1 μg/ml was used as a source of control antigens ...
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bioRxiv - Immunology 2022Quote: ... IFN-γ secreting cells were detected by Human IFN-γ ELISpot Basic Kit (ALP) (Mabtech AB, 3420-2A). The plates were developed using the AP Conjugate Substrate Kit (Biorad ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated antibody and Streptavidin-HRP were added successively after cell lysis.Then AEC Peroxidase Substrate were added and spots counted using an ELISpot plate reader (Mabtech IRIS™ Fluorospot Reader, Mabtech). Spot numbers were evaluated using Mabtech Apex™ software v.1.1.52.121.
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bioRxiv - Immunology 2022Quote: ... Biotinylated antibody and Streptavidin-HRP were added successively after cell lysis.Then AEC Peroxidase Substrate were added and spots counted using an ELISpot plate reader (Mabtech IRIS™ Fluorospot Reader, Mabtech). Spot numbers were evaluated using Mabtech Apex™ software v.1.1.52.121.
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bioRxiv - Immunology 2022Quote: ... following manufacturer’s instructions (Mabtech).
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bioRxiv - Immunology 2022Quote: Pre-coated Ferret IFNγ ELISpot (ALP) plates (Mabtech, Nacka Strand, Sweden) were used according to the manufacturers protocol ...
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bioRxiv - Cell Biology 2022Quote: For the ELIspot a commercial kit (Mabtech) was used ...
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bioRxiv - Cancer Biology 2022Quote: ... was coated with IFNΨ capture antibodies (AN18; Mabtech). 2 × 105 lymphocytes isolated from treatment-responsive mice were incubated with 5 μg/mL of the different mutant peptides pools to which 20,000 activated BMDCs were added ...
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bioRxiv - Cancer Biology 2022Quote: ... IFNΨ secretion was assessed biotinylated anti-mouse IFNΨ (R4-6A2; Mabtech) and imaged using an ELISPOT reader (AID Diagnostika) ...
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bioRxiv - Cancer Biology 2022Quote: ... were coated with an IFN-γ capture antibody (1-D1K, Mabtech) at 4°C overnight prior to the co-culture ...
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bioRxiv - Cancer Biology 2022Quote: ... and Streptavidin-HRP (Mabtech). ELISpot plates were read out on an ImmunoSpot S6 Ultra-V Analyzer using Immunospot software 5.4.0.1 (CTL-Europe).
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bioRxiv - Cancer Biology 2022Quote: ... development was performed with an IFN-γ- detection antibody (7-B6-1-biotin, Mabtech) and Streptavidin-HRP (Mabtech) ...
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bioRxiv - Immunology 2022Quote: ... ELISpot plates were coated with IFN-γ capture antibody 1-DK1 (Mabtech) and incubated with cells ...
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bioRxiv - Immunology 2022Quote: ... anti-IFN-γ 7-B6-1 (biotinylated, Mabtech) as well as streptavidin-horseradish complex was added for visualization.
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bioRxiv - Immunology 2022Quote: Interferon gamma production was measured using the ELISpot Plus: Mouse IFN-γ (ALP) (Mabtech). Splenocytes were counted and 250,000 cells were plated per well into pre-covered plates and stimulated overnight with 10 μg/mL SARS-CoV-2 (S1 ...
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bioRxiv - Immunology 2022Quote: ... Splenocytes were counted and 250,000 cells were plated per well into pre-covered plates and stimulated overnight with 10 μg/mL SARS-CoV-2 (S1) peptide pool (Mabtech) or medium as negative control or PHA 2 μg/mL as positive experimental control ...
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bioRxiv - Immunology 2022Quote: ... Human Granzyme B ELISABASIC kit (HRP) (MabTech) following manufacturers instruction ...
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bioRxiv - Immunology 2022Quote: ... Human Perforin ELISABASIC kit (HRP) (MabTech), Human Granzyme B ELISABASIC kit (HRP ...
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bioRxiv - Immunology 2022Quote: ... The assay was performed following manufacturer’s instructions (Mabtech, Nacka Strand, Sweden). An RBD peptide pool composed by 132 out of the 338 peptides covering the whole Spike protein was used for T cell stimulation ...
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bioRxiv - Immunology 2022Quote: ... we developed the spots by adding 70 µL/well of BCIP/NBT-plus substrate (Mabtech #3650-10) to the wells ...
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bioRxiv - Immunology 2022Quote: ... and BVD6-24G2 anti-IL4 (Mabtech #3311-6-250) detection antibody ...
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bioRxiv - Immunology 2022Quote: IFNγ and IL4 ELISpot assay was performed using Mouse IFNγ ELISPOT basic kit (ALP) and Mouse IL4 ELISPOT basic kit following the manufacturer’s instructions (Mabtech, VA, USA). For cell activation control ...
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bioRxiv - Immunology 2022Quote: ... coated with AN18 IFNγ (1 µg/ml, Mabtech #3321-3-250;) and 11B11 IL4 (1 µg/ml ...
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bioRxiv - Immunology 2022Quote: A fluorospot 96-well plate from a Human INF-γ/IL-2 FluorospotPLUS kit (Mabtech AB, Sweden) was washed x3 with PBS and then blocked with optimizer media-10% FCS according to manufacturer description at room temperature until use ...
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bioRxiv - Immunology 2022Quote: ... Precoated monkey IFN-γ ELISPOTplus plates (Mabtech, Cincinnati, OH) were blocked with R10 (RPMI 1640 supplemented with 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... we washed off the cells and developed the plates using biotinylated R4-6A2 anti-IFN-γ (Mabtech #3321-6-250) and BVD6-24G2 anti-IL4 (Mabtech #3311-6-250 ...
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bioRxiv - Microbiology 2022Quote: ... all at 10 µg/ml except for the CEF pool (CEF extended peptide pool for human CD8 T cells, Mabtech) used at 5 µg/ml and cultured overnight at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... Elispot plates were pre-coated with anti-IFNγ primary antibody and after cell-incubation IFNγ revealed using anti-IFNγ secondary antibody conjugated with biotin (both from Mabtech) as described (Cardinaud et al. ...
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bioRxiv - Immunology 2022Quote: ... wells were coated with human anti-IFN-γ monoclonal antibodies (1-D1K, Mabtech Sweden) at a concentration of 5μg/ml and diluted with the same coating buffer ...
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bioRxiv - Immunology 2022Quote: ... Monkey IFN-γ ELISpot plates (Mabtech) were used ...
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bioRxiv - Immunology 2022Quote: ... DMSO (peptide solvent) and anti CD3 (Mabtech) used for negative and positive control ...
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bioRxiv - Immunology 2022Quote: ... followed by 2 hours of incubation at 37°C with biotinylated anti-human IFN-γ monoclonal antibodies (clone 7-B6-1, Mabtech) diluted at 1μg/ml in PBS ...
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bioRxiv - Immunology 2022Quote: ... and incubated for 2 h with an IFN-γ detection antibody (1 μg/ml; clone mAb-7B6-1; Mabtech) followed by 1 h incubation with AP-conjugated streptavidin (1:1000 in PBS ...
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bioRxiv - Immunology 2022Quote: ... Secreted mouse IFN-γ was captured by anti-mouse IFN-γ (AN18) monoclonal antibody (Mabtech, Nacka Strand, Sweden) precoated on 96-well nitrocellulose membrane plates (Merk Millipore ...
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bioRxiv - Immunology 2022Quote: ... Anti-IFN-γ coating antibody (10 µg/ml in PBS; clone 1-D1K; Mabtech, Nacka Strand, Sweden) was then added and the plates incubated overnight at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... followed by 1 h incubation with AP-conjugated streptavidin (1:1000 in PBS, Mabtech). Plates were then washed and visualized using the VECTASTAIN® Elite® ABC-HRP kit according to the manufacturer’s instructions (Mabtech) ...
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bioRxiv - Immunology 2022Quote: ... Plates were then washed and visualized using the VECTASTAIN® Elite® ABC-HRP kit according to the manufacturer’s instructions (Mabtech). Antigen-specific T cell responses were quantified by subtracting the number of spots in unstimulated cells from the peptide stimulated cells ...
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bioRxiv - Immunology 2022Quote: ... and 11B11 IL4 (1 µg/ml, Mabtech #3311-3-250) coating antibody ...
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bioRxiv - Immunology 2022Quote: A human IFNγ ELISpotplus kit (MABTECH AB, Nacka Strand, Sweden) was used in accordance with the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Spleens from immunized animals were removed at Day 51 for the mice and at Day 110 for the hamsters and splenocytes were isolated for protein S-specific T-cell detection with the ELISpotPLUS mouse IFN-γ or ELISpotPLUS hamster IFN-γ kits (Mabtech) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Spot-forming units (SFU) were quantified using a mouse IFN-γ ELISpot kit (Mabtech, Stockholm, Sweden) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and cellular responses were determined on fresh PBMCs at Charles River Laboratories using a monkey IFN-γ ELISpot kit (Mabtech) at Weeks 1 ...
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bioRxiv - Cancer Biology 2022Quote: ... The production of human IFNα (Mabtech), IFNβ (PBL Interferon Source) ...