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Citations for Mabtech :
451 - 500 of 585 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: Ex vivo IFNγ ELISpot assays were performed according to manufacturers’ instructions (Mabtech) on freshly isolated PBMC plated in triplicates at 2×105 PBMC per well ...
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bioRxiv - Microbiology 2020Quote: ... or IgA (Cat. 3860-1AD-6, Mabtech, USA) ranging from 1.2 μg/ml to 0,0047 μg/ml ...
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bioRxiv - Immunology 2020Quote: Rhesus macaque PBMCs were tested with commercially available NHP IFNγ and IL-4 ELISpot assay kits (Mabtech). Cryopreserved rhesus macaque PBMCs were thawed in pre-warmed AIM-V media (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ELISpot assays were performed with mouse IFNγ ELISpotPLUS kits according to the manufacturer’s instructions (Mabtech). A total of 5 × 105 splenocytes was ex vivo restimulated with the full-length S peptide mix (0.1 µg/mL final concentration per peptide ...
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bioRxiv - Immunology 2020Quote: T cell responses were assessed by ELISPOT assay using a Human IFNγ ELISPOTPro kit (Mabtech, NS, Sweden) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... plates were washed and incubated with anti-IgG-biotin and/or anti-IgA-biotin (Mabtech) for 2 hours at room temperature ...
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bioRxiv - Immunology 2020Quote: Rhesus macaque PBMCs were tested with commercially available NHP IFNγ and IL-4 ELISpot assay kits (Mabtech, Sweden). Cryopreserved rhesus macaque PBMCs were thawed in pre-warmed AIM-V media (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: Murine ELISpot assays were performed with mouse IFNγ ELISpotPLUS kits according to the manufacturer’s instructions (Mabtech). A total of 5 × 105 splenocytes was ex vivo restimulated with the full-length S peptide mix (0.1 μg/mL final concentration per peptide ...
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bioRxiv - Immunology 2020Quote: ... Plates were then washed 6x with PBS and 0.5μg/mL of Streptavidin-ALP (Mabtech) was added to IFN-γ plates at 100μL/well ...
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bioRxiv - Immunology 2020Quote: ... and immediately washed 6x with 200μL ddH2O and then coated with 100μL/well of PBS + 15μg/mL primary anti-human granzyme B antibody (clone GB10, Mabtech) overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... were either directly coated with 100μL/well of PBS + 0.5μg/mL primary anti-human IFN-γ antibody (clone 1-D1K, Mabtech) overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Biotinylated secondary IFN-γ antibody (clone 7-B6-1, Mabtech) at 0.5μg/mL in PBS ...
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bioRxiv - Immunology 2020Quote: ... and 1μg/mL of Streptavidin-ALP (Mabtech) was added to granzyme B plates at 100μL/well and incubated for 1 hour ...
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bioRxiv - Immunology 2020Quote: ... or biotinylated secondary anti-granzyme B antibody (clone GB11, Mabtech) at 1.0μg/mL in PBS was added to the plates to a final volume of 100μL and incubated for 1 hour in the dark ...
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bioRxiv - Immunology 2020Quote: An ELISPOT assay was performed with the Monkey IFN-γ ELISPOT Kit (Mabtech, Cincinnati, OH, USA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Plates were then washed a further six times and developed for 20 min with BCIP/NBT Substrate (Mabtech). All assays were performed in duplicate ...
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bioRxiv - Immunology 2020Quote: ... and incubated for 2 hr at room temperature with biotinylated anti-IFN-γ (clone mAb-7B6-1; 1 μg/ml Mabtech). After six further washes ...
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bioRxiv - Immunology 2020Quote: ... pre-coated with anti-IFN-γ (clone 1-D1K; 15 μg/ml; Mabtech). Test wells were supplemented with overlapping peptides spanning Prot_E ...
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bioRxiv - Immunology 2020Quote: ... a 1:1,000 dilution of alkaline phosphatase-conjugated streptavidin (Mabtech) was added for 1 hr at room temperature ...
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bioRxiv - Microbiology 2020Quote: An IFNγ ELISpot assay was used to estimate the frequency and IFNγ production capacity of SARS-CoV-2-specific T cells in PBMCs using a human / simian IFNγ kit (MabTech, Nacka. Sweden), as described previously 46 ...
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bioRxiv - Microbiology 2020Quote: ... PBMCs or pDCs we used the Human ELISA development Kits from Mabtech for IFN-α pan (#3425-1A-20) ...
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bioRxiv - Immunology 2020Quote: ... In some experiments an IL-5 development ELISA was used (Mabtech). Measured concentrations were then adjusted to pg cytokine per million PBMC at the start of the assay ...
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bioRxiv - Immunology 2020Quote: ... and incubated with 50 μL of biotinylated anti-porcine IFN-γ mAb P2C11 (1:1000 dilution; Mabtech) after overnight incubation ...
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bioRxiv - Immunology 2020Quote: ... plates were coating with 50 μL of anti-porcine IFN-γ mAb pIFNγ-I (5 µg/ ml; Mabtech) and incubated with 50 μL of biotinylated anti-porcine IFN-γ mAb P2C11 (1:1000 dilution ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed 6X with PBS and incubated with 50 µl of streptavidin-alkaline phosphatase conjugate (1:1000 dilution; Mabtech) diluted in PBS 1% FBS ...
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bioRxiv - Immunology 2020Quote: ... were coated with 50 μl of anti-mouse IFN-γ mAb AN18 (5 µg/ ml; Mabtech) overnight at 4°C before being washed and blocked with cRMPI 10% FBS for 1 hr at 37C ...
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bioRxiv - Immunology 2020Quote: Assessment of the IFN-γ T cell response was performed using the Mouse IFN-γ ELISpotPLUS kit (Mabtech) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... Foci were counted using an AID ELISpot reader from Mabtech. The cytotoxic concentrations that reduced cell growth by 50% (CC50 ...
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Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... The IFNγ ELISpot assay was performed following the Mabtech Human IFNγ ELISpotBasic protocol (Mabtech, Cincinnati, OH) using 96-well PVDF ELISpot plates from Millipore ...
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Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... T cells were resuspended at a concentration of 3×105 cells per milliliter and 100 μl (3×104 cells/well) were transferred to IFNγ-antibody coated 96-well ELISpot plates (5μg/ml anti-IFNγ mAb 1-D1K, Mabtech, Stockholm, Sweden). Individual 9mer peptide dilutions were done in a separate 1 ml deep-well 96-well plate at a final concentration of 2μg/ml and 100 μl of this dilution was added to T cell-containing wells to give a final peptide concentration of 1μg/ml ...
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bioRxiv - Immunology 2020Quote: ... Results of ELISpot and Fluorospot assays were evaluated using an IRIS-reader and analyzed by IRIS software version 1.1.9 (Mabtech AB). The results were expressed as the number of spots per 300 000 seeded cells after subtracting the background spots of the negative control ...
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bioRxiv - Immunology 2020Quote: ... The SARS-CoV-2 S2 N defined peptide pool contains 41 synthetic peptides binding to human HLA derived from the S and N proteins of the SARS-CoV-2 virus (#3620-1, Mabtech AB) 20 ...
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bioRxiv - Immunology 2020Quote: ... M ORF3a and ORF7a proteins (#3622-1, Mabtech AB) 19 ...
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bioRxiv - Immunology 2020Quote: The SARS-CoV-2 S1 scanning pool contains 166 peptides from the human SARS-CoV-2 virus (#3629-1, Mabtech AB). The peptides are 15-mers overlapping with 11 amino acids ...
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bioRxiv - Immunology 2020Quote: IFN-γ and IL-2 secreting T cells were detected using Human IFN-γ/IL-2 SARS-CoV-2 FluoroSpotPLUS kits according to the manufacture’s protocol (Mabtech AB). The plates pre-coated with capturing monoclonal anti-IFN-γ and anti-IL-2 were incubated overnight in RPMI-1640 medium containing 10% FCS supplemented with a mixture containing the SARS-CoV-2 peptide pool (scanning or defined pools) ...
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bioRxiv - Immunology 2020Quote: ... were measured using the Human IgG SARS-CoV-2 RBD ELISpotPLUS (ALP) kit according to the manufacturer’s protocol (Mabtech AB).
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bioRxiv - Immunology 2020Quote: ... supplemented with the TLR7 and TLR8 agonist imidazoquinoline resiquimod (R848, 1 µg/ml; Mabtech AB, Nacka, Sweden), and recombinant human IL-2 (10 ng/ml ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 100 μL of the TMB ELISA substrate (Mabtech, Nacka Strand, Sweden) was dispensed into the wells and incubated for 30min at 4°C ...
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bioRxiv - Immunology 2020Quote: Immunoglobulins (Ig) in culture supernatants were measured using a human IgG ELISA development kit (Mabtech) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: IFNy ELISpot assay of PBMCs was performed using the Monkey IFNy ELISpot PRO kit (Mabtech Monkey IFNy ELISPOT pro, #3421M-2APT) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The stimulated splenocytes were analyzed for IFN-γ responses using the mouse IFN-γ ELISpot Plus kit (Mabtech AB, Sweden). Spot-forming cells (SFU ...
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bioRxiv - Microbiology 2020Quote: ... Spot-forming cells (SFU) were measured in a IRIS™ ELISpot reader using the APEX™ software from Mabtech AB ...
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bioRxiv - Immunology 2020Quote: ... The following morning plates were washed with 1x PBS-T before being coated with 3 μg/mL streptavidin-ALP secondary antibody (Mabtech, clone 7-B6-1) and incubated for 2 hours at room temperature ...
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bioRxiv - Immunology 2020Quote: ELISpot plates were coated with 10 μg/mL anti-human IFN-γ coating antibody (Mabtech, clone 1-D1K) and incubated overnight at 4C° ...
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bioRxiv - Biochemistry 2020Quote: ... serum samples were centrifuged at 10000 rpm for 20 minutes at 4 °C and supernatants were analyzed by human ApoA-I ELISA (3710-1HP-10, Mabtech Inc.), as well as by denaturant and native western blot (procedure is described in the Supplementary Information file).
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bioRxiv - Immunology 2020Quote: ... cells were removed by washing (using PBS and PBS-Tween 0.02% solution) and 100 µl of biotinylated secondary anti-IFN-γ antibody (clone 7-B6-1, Mabtech) in blocking buffer (PBS 0.5% BSA ...
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bioRxiv - Immunology 2020Quote: ... and precipitating TMB substrate (Mabtech, #3651-10). Wells were activated with 15%(v/v ...
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bioRxiv - Immunology 2020Quote: ... Alkaline phosphatase conjugated anti-human IgG (clone MT78; Mabtech Stockholm, Sweden) and p-nitrophenyl phosphate substrate (Thermo Fisher ...
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bioRxiv - Immunology 2020Quote: ... following the manufacture’s instructions (Mabtech, VA, USA). Frozen splenocytes or lung cells were thawed and seeded on the ELISPOT plate (100-300 k cells per well ...
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bioRxiv - Immunology 2020Quote: ... then washed with PBS-Tween 0.01% before to add anti-mouse IgG antibody (Mabtech – 1/500 dilution). After the last washing ...