Labshake search
Citations for Mabtech :
51 - 100 of 585 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2024Quote: ... washed and coated overnight with anti-IFN-γ coating antibody (mAb-1-D1K, Mabtech, Cat. 3420-3-1000). Plates were washed and T cells were co-cultured with autologous LCL in RPMI/ 10% FCS/ 1% Pen/Strep at an effector ...
-
bioRxiv - Immunology 2024Quote: ... wells were washed and incubated with Streptavidin-ALP (Mabtech, Cat. 3310-10) for 1 hour ...
-
bioRxiv - Immunology 2024Quote: TNFα ELISA was performed with the supernatant from T cell and LCL co-culture as described above using the human TNFα ELISABASIC kit (Mabtech, Cat. 3512-1H-6), following manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... Wells were washed and incubated for 2 hours with biotinylated anti-IFN-γ detection antibody (mAb-7-B6-1-Biotin, Mabtech, Cat. 3420-6-250). Afterwards ...
-
bioRxiv - Immunology 2024Quote: ... Wells were then washed again and spots were developed with filtered substrate solution BCIP/ NBT-plus (Mabtech, Cat. 3650-10). When distinct spots were visible ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 5-bromo-4-chloro-3- indolyl-phosphate/nitro blue tetrazolium substrate solution (Mabtech). The colour reaction was stopped by addition of water and IFN-γ spots counted with an AID ELISpot Reader (Autoimmun Diagnostika Gmbh ...
-
bioRxiv - Neuroscience 2024Quote: ... Their concentrations were determined using a Human IgG ELISA development kit (Mabtech, Sweden).
-
bioRxiv - Immunology 2024Quote: ... were coated with anti-human IFNγ (1-D1K; Mabtech), and cells were plated in triplicate at 1 × 105 cells per well ...
-
bioRxiv - Immunology 2024Quote: ... which were previously (1-7 days) coated with 4 μg/mL of anti-IFN-γ antibody (clone 1-D1k, Mabtech) and blocked by incubating at least for 1 hour with RPMI medium containing 10% human serum ...
-
bioRxiv - Immunology 2024Quote: ... and plates were incubated with biotinylated IFNγ monoclonal antibodies (MAb) (7-B6-1; Mabtech) for 2 h at 37°C and developed as described previously39 ...
-
bioRxiv - Immunology 2024Quote: ... Tetanus toxin and Ebola (CEFTA, Mabtech Inc) were used as control antigens ...
-
bioRxiv - Immunology 2024Quote: ... An antibody mixture containing anti-IFNγ (7-B6-1-FS-BAM) (Mabtech) was prepared in PBS with 0.1% BSA and incubated for 2 hours at room temperature ...
-
bioRxiv - Immunology 2024Quote: ... TMB substrate (Mabtech, Cat# 3651-10) was used to visualize the presence of the viral antigen.
-
bioRxiv - Immunology 2024Quote: ... the plates were washed and TMB substrate (Mabtech, Cat# 3651-10) was used to visualize the presence of virus specific antibodies.
-
bioRxiv - Immunology 2024Quote: Fecal samples collected from female mice at 5 weeks of age were assayed for immunoglobulin and Lcn-2 content using ELISA kits (Mabtech; R&D Systems). Protein was extracted from fecal samples by manual homogenization of 2-3 pellets in PBS + 0.05% Tween20 followed by 4 hr of agitation at 4°C ...
-
bioRxiv - Immunology 2024Quote: Fluorospot cytokine assay was performed at day 4 after T cell activation according to the manufacturer’s instructions (Mabtech). Briefly ...
-
bioRxiv - Immunology 2024Quote: ... ELISPOT plates were coated with anti-IFN-γ antibody (Mabtech, clone 1-D1K ...
-
bioRxiv - Immunology 2024Quote: HIV IFN-γ ELISPOT assays were performed following the manufacturer’s instructions (Mabtech ELISPOT Flex Human IFN-γ). In brief ...
-
bioRxiv - Immunology 2024Quote: ... Plates were washed 5x again and incubated with Streptavidin-ALP (Mabtech) for 1 hour ...
-
bioRxiv - Immunology 2024Quote: ... and IL-17 (Mabtech, clone: MT44.6) in PBS (Gibco ...
-
bioRxiv - Immunology 2024Quote: ... the plates were coated with a capture antibody cocktail containing 10 µg/mL mouse anti-human of each IFN-γ (Mabtech, clone: 1-D1K), IL-5 (Mabtech ...
-
bioRxiv - Immunology 2024Quote: ... fluorescence enhancer (Mabtech, 3641-F10) was added and removed after 15 minutes of incubation at RT ...
-
bioRxiv - Immunology 2024Quote: ... Plates were air-dried and measured using a (Mabtech IRIS Fluorospot/ELISpot Reader). A cytokine response was considered positive when the following three criteria were met ...
-
bioRxiv - Immunology 2024Quote: ... IL-5 (Mabtech, clone: TRFK5), and IL-17 (Mabtech ...
-
bioRxiv - Immunology 2024Quote: ... dilution 1:6000)- or alkaline phosphatase (ALP, clone MT20, Mabtech, dilution 1:1000)-conjugated secondary IgA-specific antibodies for IgA and sIgA ...
-
bioRxiv - Immunology 2024Quote: The amounts of IgA and sIgA were measured by ELISA protocols including capture mAbs anti-human IgA (clone MT57, Mabtech) for IgA and anti-human secretory component (clone SC-05 ...
-
bioRxiv - Immunology 2024Quote: ... native human IgA from Mabtech and sIgA isolated from healthy donor saliva were used ...
-
bioRxiv - Immunology 2024Quote: ... for 15 minutes was performed and the fluorescent spots were counted with the IRIS Fluorospot reader (Mabtech).
-
bioRxiv - Immunology 2024Quote: ... The plates were then washed and incubated with anti-BAM-490 (Mabtech) for 1 hour at room temperature ...
-
bioRxiv - Immunology 2024Quote: Plates were coated overnight at 4°C with an antibody mixture containing mouse anti-human IFNγ (Mabtech, 1-D1K). The coated plates were then prepped with stimuli as follows ...
-
bioRxiv - Immunology 2024Quote: ... A final wash and incubation with a fluorescence enhancer (Mabtech) for 15 minutes was performed and the fluorescent spots were counted with the IRIS Fluorospot reader (Mabtech).
-
bioRxiv - Immunology 2024Quote: ... were coated with anti-human IFNγ monoclonal antibody (clone 1-DIK, Mabtech, 3420-3-1000) at 0.5 μg/well overnight at 4°C ...
-
bioRxiv - Immunology 2024Quote: ... and incubated with biotinylated anti-human IFNγ monoclonal antibody (clone 7-B6-1, Mabtech, 3420-6-250) at 0.2 μg/well for 2 h ...
-
bioRxiv - Immunology 2024Quote: ... ELISpot assays were performed using Monkey IFNγ ELISpotPLUS kits (MabTech, cat# 3241M-4HPW). Precoated 96-well ELISpot plates were blocked with RPMI + 10% FBS for 2 hours at room temperature ...
-
bioRxiv - Immunology 2024Quote: ... 1×104 CD3+ T cells/well were plated in pre-coated IFNγ/IL2/TNF Fluorospot plates (Mabtech) and cultured overnight in medium alone (negative control ...
-
bioRxiv - Immunology 2024Quote: ... Plates were developed using BCIP/NBT-plus for ALP reagents (Mabtech) and read on ImmunoSpot Analyzer (Cellular Technology Limited) ...
-
bioRxiv - Immunology 2024Quote: Anti-mouse IgG conjugated with biotin (Mabtech) was added at a 1:2000 dilution ...
-
bioRxiv - Immunology 2024Quote: ... Para-nitrophenylphosphate (Mabtech) was added to each well and incubated for 60 mins at RT to develop in the absence of light ...
-
bioRxiv - Immunology 2024Quote: ... were coated with an anti-IL-12p40 capture antibody (2 μg/ml; Mabtech clone MT86/221) and incubated overnight at 4°C ...
-
bioRxiv - Immunology 2024Quote: ... IL-12p70 levels were quantified using a commercial ELISA kit (Mabtech). Nunc MaxiSorp 96-well plates (BioLegend ...
-
bioRxiv - Immunology 2024Quote: ... plates were incubated for 1h at RT with a biotin conjugated anti-IL-12p70 detection antibody (1 μg/ml; Mabtech clone MT704) diluted to 1 μg/ml before the process was repeated using streptavidin conjugated to alkaline phosphatase (Mabtech ...
-
bioRxiv - Immunology 2024Quote: ... diluted to 1 μg/ml before the process was repeated using streptavidin conjugated to alkaline phosphatase (Mabtech; 1:1000 dilution of stock protein). Following further washing with PBS containing 0.05% Tween-20 ...
-
bioRxiv - Immunology 2024Quote: ... we also performed an IgG ELISpot assay to enumerate B cells that are secreting human IgG in response to the Sars-CoV-2 receptor binding domain (RBD) (3850-4HPW-R1-1; Mabtech, Nacka Strand, Sweden). Moreover ...
-
bioRxiv - Immunology 2024Quote: ... or granzyme B (3486n-4APW-P1-1; Mabtech, Nacka Strand, Sweden) in response to a pool of SARS-CoV-2 peptides ...
-
bioRxiv - Immunology 2024Quote: ... wells with PBMCs alone were used as negative control and PBMCs treated with an anti-human anti-CD3 antibody (mAb CD3-2, #3420-4HST-10, Mabtech, Nacka Strand, Sweden) overnight served as positive control ...
-
bioRxiv - Immunology 2024Quote: We used two distinct ELISpot assays to quantify the number of immune cells that secrete either IFN-γ (3420-4AST-P1-1; Mabtech, Nacka Strand, Sweden) or granzyme B (3486n-4APW-P1-1 ...
-
bioRxiv - Immunology 2024Quote: ... ELISPOT plates were pre-coated with anti-IFNγ primary antibody and after cell-incubation IFNγ revealed using anti-IFNγ secondary antibody conjugated with biotin (both from Mabtech) as described 24 ...
-
bioRxiv - Immunology 2024Quote: ... except for the CEF pool (Mabtech) used at 5 µg/ml ...
-
bioRxiv - Immunology 2024Quote: ... Non-HIV common peptides (from HCMV, EBV and influenza virus) were obtained from Mabtech (PepPool: CEF (CD8), human ...
-
bioRxiv - Molecular Biology 2024Quote: ... streptavidin-alkaline phosphatase (Mabtech) and 5-bromo-4-chloro-3- indolyl-phosphate/nitro blue tetrazolium substrate solution (Mabtech) ...