Labshake search
Citations for PerkinElmer :
401 - 450 of 655 citations for DnaJ Hsp40 Homolog Subfamily C Member 10 DNAJC10 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... 1 mM MnCl2) supplemented with 10 μM ATP and 2 μCi γ-32P-ATP (blu002z250uc, Perkin Elmer) in 15μL reactions ...
-
bioRxiv - Cell Biology 2022Quote: ... were added to cells at a final concentration of 10 μM using a Janus Automated Workstation (PerkinElmer) equipped with a pin tool (V&P Scientific) ...
-
bioRxiv - Microbiology 2022Quote: ... Bioluminescence imaging was performed after 10-20 minutes using an IVIS Lumina II or Spectrum system (PerkinElmer). Image acquisition settings were adjusted dependent on signal saturation (exposure time ...
-
bioRxiv - Cancer Biology 2023Quote: ... by intraperitoneal injection and then imaged 10 min later using an IVIS™ Spectrum imaging system (PerkinElmer). Body weight and tumor volume were measured 1-2x weekly.
-
bioRxiv - Immunology 2023Quote: ... 10 µl of unpurified transfection supernatant diluted 1:200 in 1x Immunoassay buffer (Perkin Elmer cat# AL000F) was pre-incubated with either 10 µl biotinylated SARS-CoV-2 spike protein (3 nM final concentration ...
-
bioRxiv - Neuroscience 2023Quote: ... 7 increasing concentrations (0.15 – 10 nM) of [3H]-SCH23390 (specific activity 83.6Ci/mmol, Perkin Elmer, #NET930025UC, Canada) for D1 binding to generate saturation curves ...
-
bioRxiv - Bioengineering 2023Quote: ... for 10 minutes and then imaged using the In Vivo Imaging System (IVIS) Spectrum imaging system (PerkinElmer) and Living Image software (PerkinElmer).
-
bioRxiv - Cell Biology 2023Quote: ... The plates were then incubated in a cell culture incubator for 1 hour at 37°C until the colour turned orange and the absorbance was measured at 450nm using a microplate reader (Perkin Elmer Wallac Victor2 1420 Multilabel Counter). Results are expressed as the percentage of cell viability per well in relation to the maximum cell viability of DMSO-treated cells ...
-
bioRxiv - Cancer Biology 2021Quote: ... Endogenous peroxidase was then blocked by incubation in Antibody Diluent/Block (PerkinElmer #72424205, Massachusetts, USA) for 10 minutes ...
-
bioRxiv - Microbiology 2020Quote: ... and the bound antibodies were detected using enhanced chemiluminescence solution (Perkin Elmer, Waltham, MA, USA). Images were acquired using the ChemoCam 6.0 ECL system (INTAS Science Imaging ...
-
bioRxiv - Microbiology 2022Quote: ... Horseradish peroxidase-conjugated goat anti-rabbit polyclonal and anti-mouse antibodies were purchased from PerkinElmer Life Sciences (Boston ...
-
bioRxiv - Microbiology 2019Quote: ... USA) Horseradish peroxidase-conjugated goat anti-rabbit and anti-mouse antibodies were purchased from PerkinElmer Life Sciences (Boston ...
-
bioRxiv - Cancer Biology 2020Quote: ... and bound primary antibodies were visualized using Western Lightning Plus-ECL (PerkinElmer Inc., Waltham, MA). The primary antibodies used in this study were as follows ...
-
bioRxiv - Neuroscience 2022Quote: ... Secondary anti-HRP conjugate antibodies were used and immunoreactivity was visualized using Western LightingTM (PerkinElmer). Immunoreactivity was developed on film or using a ChemiDoc™ imaging system (Biorad) ...
-
bioRxiv - Molecular Biology 2020Quote: ... 10 images of each section were randomly collected and analyzed by the Columbus v.2.4.2 software (Perkin Elmer) with an algorithm adapted for Oil Red-O stained sections ...
-
bioRxiv - Cell Biology 2021Quote: ... 3 10-minute TBST washes were performed followed by Western Lightning plus ECL Enhanced Chemiluminescence (Perkin Elmer, MA). Proteins were detected on X-ray film (Fuji ...
-
bioRxiv - Cell Biology 2022Quote: ... 3 mL of the hexane layer was added to 10 mL UltimaGold liquid scintillation cocktail (PerkinElmer, Waltham, USA) and measured on a TriCarb 2800TR liquid scintillation counter (PerkinElmer ...
-
bioRxiv - Zoology 2019Quote: ... Three tubes (final volume of 150 µL) were prepared for each sample: two containing 160 nM cortisol (10% 1,2,6,7-3H-cortisol, Perkin Elmer, Waltham ...
-
bioRxiv - Biochemistry 2020Quote: ... pH 7.4) with 10 μM 2-deoxy-Glucose and 0.5 μCi/mL 3H-2-deoxy-glucose (Perkin Elmer). Cytocholasin B (5 μM ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... tritiated [10-3H(N)]-JH III (specific activity 19.4 Ci/nmol, Perkin Elmer Life Sciences, Waltham, MA, USA), and synthetic juvenile hormone-III (Fluka ...
-
bioRxiv - Cancer Biology 2021Quote: ... Plates were incubated for 10 minutes at room temperature and luminescence determined using an EnVision Multilabel reader (PerkinElmer) and the readings taken as the measure of cell growth ...
-
bioRxiv - Cancer Biology 2021Quote: ... was added and incubated for 10 min before luminescence was measured with a multimode microplate reader (PerkinElmer, EnVision). Data were fitted into an inhibitor-response model to get IC50 values.
-
bioRxiv - Cancer Biology 2022Quote: ... supplemented with 10% Fetal Bovine Serum (100 uL) in 384-well CellCarrier Ultra plates (PerkinElmer, Waltham, Massachusetts, USA). For time-lapse microscopy ...
-
bioRxiv - Biochemistry 2022Quote: ... 100 μM cold ATP and 1 μCi [γ-35P] ATP (6000 Ci/mmol,10 mCi/ml, Perkin Elmer) with recombinant proteins (200-500 ng ...
-
bioRxiv - Molecular Biology 2022Quote: ... 10 μL immunoprecipitated enzyme were mixed with 300 μM synthetic peptide and 50 μM [14C]-acetyl-CoA (PerkinElmer) in acetylation buffer (50 mM HEPES pH 8.5 ...
-
bioRxiv - Biochemistry 2023Quote: ... and 10 mM DTT) were combined in a 384 well plate (PerkinElmer ProxiPlate-384 Plus F black, #6008260) and data gathered on a Envision 2150 plate reader (PerkinElmer ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... and biotinylated-JQ1 at 20nM was added in 10 µL to 384-well plates (AlphaPlate-384, PerkinElmer, USA). Plates were spun down at 150x g ...
-
bioRxiv - Cancer Biology 2023Quote: ... and imaged live for 10 hours using the Operetta High Content Screening System (Perkin Elmer, see section 3.2.6) at 37 °C and 5% CO2 at intervals of 20 mins ...
-
bioRxiv - Molecular Biology 2024Quote: ... anti-FLAG donor and protein G coated acceptor beads (10 ng/μl final concentration; AS103D and AL102C, PerkinElmer). For the competitive assay with CD81-GFP and antiHER2 EVs ...
-
bioRxiv - Biochemistry 2024Quote: ... 100 nM Ocr was mixed with 128 µM ATP supplemented with 10 nM [α- 32 P]-ATP (PerkinElmer) in the reaction buffer ...
-
bioRxiv - Cell Biology 2021Quote: ... were then added to the COS-7 cells in the presence of 50 μg/ml PI and imaged immediately at 37°C with 5% CO2 using a spinning disk confocal microscope (UltraVIEW VoX, PerkinElmer with a 40X 1.3 N.A. oil objective). Images were acquired at 1 frame/20s and analyzed using NIH ImageJ software ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were imaged at 1 frame/30 s for 4 h at 37°C with 5% CO2 using a spinning disk confocal microscope (UltraVIEW VoX, PerkinElmer with a 63X 1.4 N.A. oil objective), followed by addition of 50 μg/ml PI (Thermo Fisher Scientific ...
-
A distinctive ligand recognition mechanism by the human vasoactive intestinal polypeptide receptor 2bioRxiv - Molecular Biology 2021Quote: CHO-K1 cells were cultured in F12 medium with 10% FBS and seeded at a density of 30,000 cells/well in Isoplate-96 plates (PerkinElmer). Twenty-four hours after transfection with the WT or mutant receptors ...
-
bioRxiv - Biochemistry 2019Quote: ... Oligonucleotides were 5’-end-labeled with [γ-32P]-ATP (10 mCi ml-1, 3000 Ci mmol-1; Perkin Elmer) using polynucleotide kinase (Thermo Scientific) ...
-
bioRxiv - Microbiology 2020Quote: ... Plates were incubated for 10 mins at room temperature and then were analyzed on the Envision plate reader (PerkinElmer). Relative light units (RLUs ...
-
bioRxiv - Microbiology 2021Quote: ... incubated for 10 min at RT and luciferase signal was quantified on a VICTOR Nivo plate reader (Perkin Elmer).
-
bioRxiv - Cancer Biology 2020Quote: ... the slides were then incubated at RT for 10 min with one of the following Alexa Fluorescent tyramides (PerkinElmer) included in the Opal 4 colour kit (NEL810001KT ...
-
bioRxiv - Microbiology 2021Quote: ... Plates were incubated for 10 min at room temperature and then were analyzed on the Envision plate reader (PerkinElmer). Relative light units (RLUs ...
-
bioRxiv - Cancer Biology 2020Quote: ... the slides were then incubated at RT for 10 min with one of the following Alexa Fluorescent tyramides (PerkinElmer) included in the Opal 4 color kit (Cat No ...
-
bioRxiv - Molecular Biology 2021Quote: CHO-K1 cells were cultured in F12 medium with 10% FBS and seeded at a density of 30,000 cells/well in Isoplate-96 plates (PerkinElmer). The WT (HA-Flag-3GSA-GIPR(22-466) ...
-
bioRxiv - Molecular Biology 2021Quote: ... CHO-K1 cells were cultured in F-12 medium with 10% FBS and seeded at a density of 30,000 cells/well in Isoplate-96 plates (PerkinElmer). The wild-type (WT ...
-
bioRxiv - Microbiology 2022Quote: ... oligonucleotides were 5’-end-labelled with [γ-32P]-ATP (10 mCi ml-1, 3000 Ci mmol-1, Perkin Elmer) with polynucleotide kinase (Thermo Scientific) ...
-
bioRxiv - Microbiology 2022Quote: ... 10 μL of lipid extracts were mixed with 2 mL of Ultima Gold™ scintillation fluid (Perkin Elmer, USA), and radioactive counts were measured using a MicroBeta2® scintillation counter (Perkin Elmer ...
-
bioRxiv - Microbiology 2022Quote: ... 10 μCi of either [1,2-3H(N)] free cholesterol or [1,2-3H(N)]cholesteryl oleate (Perkin Elmer, Boston, MA) were mixed with absolute ethanol ...
-
bioRxiv - Microbiology 2022Quote: ... Reactions contained 10 μL rabbit reticulocyte lysate with 500 ng of pcDNA T7 expression plasmid and 0.5 μL [35S] methionine (PerkinElmer). Reactions were incubated at 30°C for 40 minutes before being chased with 2 μl of 50 mg / mL unlabelled methionine ...
-
bioRxiv - Microbiology 2023Quote: ... Reactions contained 10 μL lysate with 250 ng of pcDNA T7 expression plasmid and 0.5 μL [35S] methionine/cysteine (PerkinElmer). Reactions were incubated at 30°C for 40 minutes chasing with 2 μL of 50 mg / mL unlabelled methionine/cysteine ...
-
bioRxiv - Biochemistry 2023Quote: ... The mice were anesthetized in a ventilated anesthesia chamber with 1.5% isofluorane in oxygen and imaged 10 min after the injection with an in vivo imaging system (IVIS, PerkinElmer). Luminescence was quantified using the Living Image software (PerkinElmer) ...
-
bioRxiv - Cell Biology 2022Quote: Candidate small RNA sequences were identified from raw sequence data using cutadapt (ver. 2.8)21 with parameters “-u -4 -a A(10)” as recommended by PerkinElmer NextFlex Combo-Seq kit ...
-
bioRxiv - Cell Biology 2023Quote: ... quantitative microscopy was performed using the Operetta HCS imaging system with a 10×/0.3 NA objective (PerkinElmer, Courtaboeuf, France). Images were analyzed using scripts developed in Acapella software (PerkinElmer ...
-
bioRxiv - Biochemistry 2023Quote: ... 10 μM purified IKK2 protein was first autophosphorylated in the presence of 100-200 nM of γ32P-ATP (PerkinElmer) in kinase assay buffer for 1 hour at 30°C ...