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Citations for PerkinElmer :
351 - 400 of 655 citations for DnaJ Hsp40 Homolog Subfamily C Member 10 DNAJC10 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... astrocytes were incubated with a mixture of 30 μM of GABA and [3H] GABA (40 μCi, specific activity = 25 Ci / mmol, PerkinElmer, 15 min, 37 ° C.). The supernatant is then removed ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The plate was incubated in the dark for 60 min at room temperature (∼22 °C) and subsequently read on an EnVision Multilabel Reader (PerkinElmer Life and Analytical Sciences), with excitation at 340 nm and measurements of emission at 615 and 665 nm ...
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bioRxiv - Genomics 2023Quote: ... was measured in triplicate and averaged at 405 nm for 45-60 minutes at 25°C on a Wallac Victor 2 1420 Multilabel Counter Plate Reader (Perkin Elmer, Waltham, MA, USA). Citrate synthase activity was standardized against the protein concentration of each sample using the Bovine Serum Albumin Assay (500-0201 ...
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bioRxiv - Immunology 2023Quote: ... A chemiluminescence assay was conducted for continuous measurement of chemiluminescence counts per second (cps) at 37°C in an Envision multimode plate reader (PerkinElmer Life Sciences, Waltham, MA), recording measurements at 1 min intervals.
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bioRxiv - Biochemistry 2021Quote: ... and then incubated with anti-FLAG antibody conjugated to APC (αFLAG-APC; PerkinElmer) diluted 1:100 in PBS + 0.1% BSA at a ratio of 30 μL labeled antibody:107 cells ...
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bioRxiv - Neuroscience 2022Quote: ... Antibody binding was detected using the enhanced chemiluminescence immunoblotting detection system (Perkin Elmer LLC WESTERN LIGHTNING PLUS-ECL ...
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bioRxiv - Cell Biology 2022Quote: ... Bound antibodies were detected with chemiluminescent substrate (Western Lightning® Plus, Perkin Elmer) and visualized with iBrightTM CL1000 (Thermo Fisher Scientific).
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bioRxiv - Immunology 2020Quote: ... The antibodies were visualized by incubation with Tyramide Red or Green (Perkin Elmer) for 10 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... The signals were detected by HRP-conjugated secondary antibodies and ECL reagent (PerkinElmer). Quantitative Western blotting was made using ImageJ ...
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bioRxiv - Genetics 2021Quote: ... horseradish peroxidase-conjugated secondary antibodies and Western Lightining® Plus-ECL (NEL105001EA, PerkinElmer) were used.
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bioRxiv - Cancer Biology 2022Quote: ... horseradish peroxidase-conjugated secondary antibodies and Western Lightining® Plus-ECL (NEL105001EA, PerkinElmer) were used ...
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bioRxiv - Immunology 2022Quote: ... Unspecific antibody binding on sorted cells was blocked with TSA blocking reagent (PerkinElmer) for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed with TBST and incubated with secondary antibody (NEF812001EA, NEF822001EA, PerkinElmer) for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... HRP-conjugated antibodies were detected using western lighting plus-ECL reagent (Perkin elmer).
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bioRxiv - Neuroscience 2023Quote: ... membranes were incubated with goat anti-rabbit HRP- labelled antibody (Perkin Elmer, # NEF812001EA) at 1/10000 in 1 % (w/v ...
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bioRxiv - Pathology 2024Quote: ... were directly coupled to antibodies while -streptavidin coated donor beads (6760002L, Perkin Elmer) were a used in combination with biotinylated antibodies ...
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bioRxiv - Neuroscience 2024Quote: ... primary antibodies followed by HRP-conjugated secondary polymer (anti-rabbit, anti-mouse [PerkinElmer]) and HRP-reactive OPAL fluorescent reagents (PerkinElmer) ...
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bioRxiv - Biochemistry 2024Quote: ... Acceptor mixtures were prepared by mixing ULight alpha-6xHIS acceptor antibody (Perkin Elmer) with 6xHIS-tagged Swc5 variants at a ratio of 1:20 and serially diluting across 13 concentrations in H66 buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Cancer Biology 2021Quote: ... see the lower phosphate competition assay above) supplemented with 10 μci/mL 32PO4 (Perkin Elmer NEX053001MC) and incubated at room temperature for 30 minutes ...
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bioRxiv - Genetics 2021Quote: ... Probes were end-labelled [1 pmol] using 10 μCi of [γ-32P]-ATP (PerkinElmer, Waltham, MA) and 10 U polynucleotide kinase (New England Biolabs ...
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bioRxiv - Molecular Biology 2020Quote: ... supplemented with 10% FBS in 96-or 384-well CellCarrier Ultra plates (PerkinElmer, Waltham, Massachusetts, USA). For time-lapse microscopy ...
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bioRxiv - Molecular Biology 2019Quote: ... 10 ng/ml doxycycline or PBS) and plates were measured with an EnSpire Reader (Perkin Elmer).
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bioRxiv - Biochemistry 2021Quote: ... The vials were cooled to room temperature and 10 ml Hionic-Fluor Scintillation Cocktail (Perkin Elmer) added to each vial and shaken thoroughly ...
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bioRxiv - Cancer Biology 2020Quote: ... was added for 10 minutes and luminescence was measured on an Envision 2104 plate reader (PerkinElmer). Subsequently ...
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bioRxiv - Immunology 2022Quote: ... Bioluminescence was measured 10 min later using a Lumina Series III In Vivo Imaging System (PerkinElmer). To evaluated the in vivo delivery capability and visualize protein expression and tissue distribution of pDNA/PP-sNp formulations ...
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bioRxiv - Biochemistry 2020Quote: ... The vials were cooled to room temperature and 10 ml Hionic-Fluor Scintillation Cocktail (Perkin Elmer) added to each vial and shaken thoroughly ...
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bioRxiv - Microbiology 2021Quote: ... in buffer A and [γ-P32]-ATP (10 mCi/mL, 3,000 Ci/mmol-Perkin Elmer # BLU002A). The labelled RNA was gel-purified as described above.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 10 μL of the cell lysates were transferred into a white 384 well plate (ProxiPlate, PerkinElmer). 5 μL of the Europium-cAMP tracer (1 to 50 dilution of Eu-tracer stock in cAMP detection buffer ...
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bioRxiv - Biochemistry 2023Quote: ... Mixture and gel slices were then combined with 10 mL of Hionic-Fluor scintillation fluid (PerkinElmer), dark-adapted overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... 2.5×10^3 LN-229 cells/well were plated in CellCarrier-96 Ultra Microplates (PerkinElmer, #6055302) 24 hours prior to the experiment ...
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bioRxiv - Biochemistry 2024Quote: ... 100 nM PARIS and 128 µM ATP supplemented with 10 nM [α-32 P]-ATP (PerkinElmer). The reaction buffer includes 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated with a D1R antagonist for 1h at 4°C in a solution equivalent to the pre-incubation buffer with the addition of 1nM [3H]SCH23390 (Perkin Elmer, USA, 85 Ci/mmol). Non-specific binding was determined by incubating sections under the same conditions with excess amount of the D1R ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated with a D2R antagonist for 1h at 4°C in a solution equivalent to the pre-incubation buffer with the addition of 4nM [3H]raclopride (Perkin Elmer, USA, 60.1 Ci/mmol). Non-specific binding was determined by incubating sections under the same conditions with excess amount of the D2R ...
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bioRxiv - Cell Biology 2021Quote: ... and imaged immediately at 37°C with 5% CO2 using a spinning disk confocal microscope (UltraVIEW VoX, PerkinElmer with a 63X 1.4 N.A. oil objective) with or without 50 μg/ml PI and/or 10 µg/ml FM1-43FX or FM4-64FX (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... and imaged immediately at 37°C with 5% CO2 using a spinning disk confocal microscope (UltraVIEW VoX, PerkinElmer with a 60X 1.4 N.A. oil objective). Images were acquired at 1 frame/20 s for 60 min and analyzed using a custom-made MATLAB script (MathWorks ...
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bioRxiv - Cell Biology 2020Quote: ... Antibody binding was visualized using ECL Plus Western Lightning reagent (Perkin Elmer NEL 102) and blots were exposed to film (Kodak BioMax MR Film ...
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bioRxiv - Neuroscience 2019Quote: ... The anti-fluorescein antibody was visualized by using the TSA Plus Fluorescein System (PerkinElmer) in accordance with the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... membranes were incubated secondary antibodies and developed using Western Lightning® Plus-ECL (PerkinElmer).
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bioRxiv - Microbiology 2023Quote: ... Secondary antibodies used were 1:10,000 of HRP-labeled α-mouse (for Flag) (PerkinElmer) or α-rabbit (PerkinElmer ...
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bioRxiv - Developmental Biology 2021Quote: ... and developed for 10 minutes with fluorescein- or Cy3-conjugated Tyramide amplication system (Perkin Elmer, Shelton, CT). Amplified staining for Aggrecan was performed without detergent (1X PBS only ...
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bioRxiv - Biochemistry 2022Quote: ... ATP concentration was varied from 0 to 1000 mM in presence of 10 nM [3H]ATP (PerkinElmer). The reaction was initiated by adding ATP to the reaction solution containing 0.005 mg/ml Cya ...
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bioRxiv - Neuroscience 2021Quote: ... and the conjugate was detected using the Fluorescein TSA Plus System (1:10, PerkinElmer, Waltham, MA, USA) for 2 h ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and 10 μL of lysate was transferred to a 384-well OptiPlate (PerkinElmer Life and Analytical Sciences). 5 μL of 1/100 dilution of the Alexa Fluor® 647-anti cAMP antibody solution in the Detection Buffer (PerkinElmer Life and Analytical Sciences ...
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bioRxiv - Microbiology 2020Quote: ... and 2.5 μCi of [γ32P]-ATP (stock of 6000 Ci/mmol 10 mCi/mL, from Perkin Elmer). Autophosphorylation reactions were performed at 30 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... and incubated for 10 min at room temperature with Opal Polymer HRP Mouse Plus Rabbit secondaries (PerkinElmer). After another wash in PBS ...
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bioRxiv - Genomics 2022Quote: ... For 6HIS-tagged proteins a 10 μl mix of 2.5 μg/ml Ni-NTA acceptor beads (PerkinElmer) and 10 μg/ml streptavidin donor beads was used ...
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bioRxiv - Physiology 2022Quote: ... and 0.01% Triton X-100) in the presence of 10 μCi [32P]-γ-ATP (Perkin Elmer Company) with a final volume of 20 μl ...
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bioRxiv - Biochemistry 2021Quote: ... and 10 μL of lysate was transferred to a 384-well OptiPlate (PerkinElmer Life and Analytical Sciences). 5 μL of 1/100 dilution of the Alexa Fluor® 647-anti cAMP antibody solution in the Detection Buffer (PerkinElmer Life and Analytical Sciences ...
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bioRxiv - Cancer Biology 2020Quote: ... and incubated for 10 min at room temperature with Opal Polymer HRP Mouse Plus Rabbit secondaries (PerkinElmer). After another wash in PBS ...
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bioRxiv - Synthetic Biology 2020Quote: ... Images were acquired within 10 minutes following intraperitoneal injection of 150mg/kg of D-luciferin (PerkinElmer #122799)