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Citations for PerkinElmer :
251 - 300 of 655 citations for DnaJ Hsp40 Homolog Subfamily C Member 10 DNAJC10 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... 1 µCi 3H-SAM and ∼1 µg of enzyme) for 1 hour at 30° C following which they are transferred to FlashPlates (Perkin Elmer) and incubated for 30 min for peptide capture ...
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bioRxiv - Cell Biology 2019Quote: ... GST-Aq-NF-κB or GST-Aq-NF-κB-ALA C-terminal peptides and 5 μCi [γ-32P] ATP (Perkin Elmer) in kinase reaction buffer (25 mM Tris-HCl ...
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bioRxiv - Microbiology 2019Quote: The membrane was hybridized overnight at 50°C with DNA oligo probes labeled with [gamma- 32P]-ATP (6000 Ci/mmol) (Perkin Elmer). 20 pmol of the DNA oligo was mixed with 7 μl of [gamma- 32P]-ATP ...
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bioRxiv - Microbiology 2020Quote: ... Cells were incubated for 16–18 hours at 37°C before Fluc activity in cell lysates was detected using a plate luminometer (EnSpire 2300 Multilabel reader by Perkin Elmer) and the Bright-Glo Luciferase Assays System (Promega) ...
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bioRxiv - Neuroscience 2020Quote: ... Imaging was performed at 37°C and 3% CO2 using an Opera Phenix™ high-content confocal microscopy system (Perkin Elmer). Images were acquired at 40x magnification using a 425 nm laser for excitation of CFP and emissions detected at 435-515 nm (CFP ...
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Surveying the landscape of tRNA modifications by combining tRNA sequencing and RNA mass spectrometrybioRxiv - Biochemistry 2019Quote: ... at 42 °C for 30 min followed by hybridization overnight at 42 °C with 4 pmol DNA probes radiolabeled with [γ-32P]ATP (PerkinElmer) and T4 Polynucleotide kinase (New England Biolabs) ...
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bioRxiv - Microbiology 2021Quote: ... Liquid cultures were incubated for seven days at 29 °C in the dark and the bioluminescence signal was measured daily with an EnVision plate reader (Perkin Elmer) three times for three seconds per well ...
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bioRxiv - Microbiology 2021Quote: ... 200 µl of the diluted cultures were transferred onto 96-well plate and OD490 at 37 °C was measured at fixed interval using Victor X4 plate reader (Perkin Elmer). For manual growth curve and CFU enumeration ...
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bioRxiv - Microbiology 2022Quote: ... at 42 °C for 30 min followed by hybridization overnight at 42°C with 4 pmol probes (Table S3) that were radiolabeled using [γ-32P] ATP (PerkinElmer) and T4 Polynucleotide kinase (New England Biolabs) ...
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bioRxiv - Synthetic Biology 2022Quote: ... 200 μL of these dilutions were then pipetted into opaque 96-well microplates and incubated at 30°C in a Victor X3 multilabel microplate reader (Perkin Elmer) that periodically measured absorbance (OD600 ...
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bioRxiv - Synthetic Biology 2022Quote: ... cells were incubated with the AAVs for 24 hours at 37°C and assayed with Britelite plus (Perkin Elmer, cat#6066766) following the manufacturer’s protocol.
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bioRxiv - Neuroscience 2023Quote: ... The choroid plexus was subsequently swiftly rinsed in cold isotope-free HEPES-aCSF containing 2 mM ouabain (4°C) and transferred to a scintillation vial and dissolved in 100 μl Solvable (6NE9100, Perkin Elmer). For the light and dark phase influx experiment ...
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bioRxiv - Microbiology 2023Quote: Growth of bacterial cultures to study phage infection or toxicity of SAMase was carried at 37°C in a 96-well format using EnSpire Multimode Plate Reader (Perkin Elmer). Overnight bacterial cultures were diluted 100-fold in LB medium with appropriate antibiotics and grown at 37°C in 10 mL of LB ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The 96-well plate was incubated for 24h at 37 °C in a Victor3 microtiter plate reader (PerkinElmer, Waltham, MA, USA), and absorbance was measured every three minutes ...
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bioRxiv - Biochemistry 2023Quote: ... Binding reactions were incubated for 2 hours and then terminated by washing the solutions over a PEI-coated GF/C filter using a cell harvester (Perkin Elmer) followed by wash steps using ice-cold buffer [50 mM HEPES ...
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bioRxiv - Immunology 2023Quote: ... B cells were lysed in water by osmotic pressure and were transferred from 96 well plates to glass fiber filtermat (8×12 Filtermat A, GF/C, 100/pk, #1450-421, Perkin Elmer). The filters were rinsed with water and sealed in plastic bags with a scintillation cocktail (OptiScint® LLT ...
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bioRxiv - Cancer Biology 2023Quote: ... then each well was incubated for 30 min at 37 °C with 5 μl/well EXPRE35S35S Protein Labeling Mix (PerkinElmer NEG072002MC), after which cells were lysed in RIPA lysis buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2023Quote: ... Bioluminescence was recorded every 30 min in DD at 25°C and 65% humidity using a TopCount Multiplate Reader (Perkin Elmer). An sCMOS camera (DMK33UX178 ...
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bioRxiv - Biochemistry 2023Quote: ... Filter-bound [3H]VUF15485 was measured by adding 25 µL Microscint-O per well to the dried GF/C-plate and radioactivity was consecutively quantified using the Wallac Microbeta counter (Perkin Elmer). The sigmoidal dose-dependent displacement curves of [3H]VUF15485 binding by unlabelled ligands were analysed using Graphpad Prism 8 ...
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bioRxiv - Biochemistry 2023Quote: ... Each binding reaction was allowed to reach equilibrium at 30 °C for 5 min prior to fluorescence polarization measurements in a Victor X5 Multilabel Plate Reader (Perkin Elmer). Each Kd measurement was calculated according to our previous work (45) ...
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bioRxiv - Biochemistry 2023Quote: ... Incubations were done at room temperature (24°C) for 4h and the end-point fluorescence reading obtained with a multimode microplate reader (Envision or EnSight, Perkin Elmer) equipped with excitation 405 nm and emission 535 nm filters ...
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bioRxiv - Cell Biology 2023Quote: ... and 1% antibiotic/antimycotic at 37°C for 30 min and then labeled with Express [35S] Labeling Mix (PerkinElmer, NEG072-007) for 15 min ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were then maintained at constant 37°C for 36 hours before moving to an Opera Phenix Plus (Revvity Perkin Elmer, at the UCSC Chemical Screening Center RRID SCR_021114) ...
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bioRxiv - Biophysics 2023Quote: ... and their proliferation was recorded at 37 °C with a Nikon Ti inverted microscope using an Ultraview spinning disk confocal system (Perkin Elmer). For determining TEM opening ...
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bioRxiv - Genomics 2019Quote: ... followed by blocking with antibody diluent/blocker (Perkin Elmer, Waltham, MA) for 30 min ...
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bioRxiv - Developmental Biology 2019Quote: ... and detected with a tyramine-amplified fluorescent antibody (Perkin Elmer, NEL753001KT).
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bioRxiv - Neuroscience 2021Quote: ... antibodies followed by fluorescent staining with Tyramide Signal Amplification system (PerkinElmer). After mounting sections in VectaShield ...
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bioRxiv - Microbiology 2020Quote: ... and acceptor antibodies were conjugated to Alpha Acceptor Beads (PerkinElmer #6760137M).
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bioRxiv - Biochemistry 2022Quote: ... 1 nM LANCE Ultra Europium anti-rabbit IgG antibody (PerkinElmer AD0082), and 50 nM LANCE Ultra Ulight-Streptavidin (PerkinElmer TRF0102 ...
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bioRxiv - Neuroscience 2023Quote: ... and HRP-labeled goat anti-rabbit antibody (1:500, PerkinElmer, NEF812001EA) for secondary detection ...
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bioRxiv - Plant Biology 2023Quote: ... The secondary antibody (Anti-Rabbit IgG [Goat], HRP-Labeled, PerkinElmer NEF812001EA) was 1:10000 in 5% TBST-milk.
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bioRxiv - Developmental Biology 2023Quote: ... and anti-DNP HRP-conjugated antibody (Perkin Elmer, Cat# FP 1129) followed by tyramide signal amplification (Akoya Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... or peroxidase-conjugated primary antibodies using Western Lightning Plus-ECL (PerkinElmer) and the chemiluminescent system AI680 (GE Healthcare ...
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bioRxiv - Cancer Biology 2021Quote: ... These 10 µl reactions were carried out in wells of 384-well Optiplate (PerkinElmer). The dilution of the control compounds was first performed in 100 % DMSO ...
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bioRxiv - Cell Biology 2021Quote: ... Images were acquired at 1 frame/6-10 s and analyzed using Volocity (PerkinElmer) and NIH ImageJ ...
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bioRxiv - Plant Biology 2020Quote: ... 40 μM ATP and 10 μCi of [α32P]-ATP (3000 Ci/mmol; Perkin Elmer). Transcription reactions were incubated 1h at room temperature on a rotating mixer ...
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bioRxiv - Cell Biology 2021Quote: ... A mixture of 10 μl of 5 μg/ml Nickel-chelate acceptor beads (PerkinElmer) and 20 μg/ml streptavidin donor beads (PerkinElmer ...
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bioRxiv - Cell Biology 2021Quote: ... Separation of 10 µl samples was carried out by HPLC (Perkin Elmer Series 200) using gradient elution on a Luna Omega Polar C18 50×3 mm ...
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bioRxiv - Genomics 2022Quote: ... For GST-tagged proteins a 10 μl mix of 2.5 μg/ml glutathione (PerkinElmer) and 5 μg/ml streptavidin donor beads (PerkinElmer ...
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bioRxiv - Cancer Biology 2019Quote: These 10 µl reactions were carried out in wells of 384-well Optiplate (PerkinElmer). The dilution of the compounds was first performed in 100% DMSO with the highest concentration at 0.5mM ...
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bioRxiv - Cell Biology 2020Quote: ... 5 microcuries (μCi) of [γ-32P]-ATP (3000 Ci/mmol, 10 mCi/ml) (PerkinElmer) in kinase buffer (20mM HEPES pH 7.5 ...
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bioRxiv - Developmental Biology 2022Quote: ... in PBS for 10 min before imaging on Opera Phenix high-content microscope (PerkinElmer) or Zeiss LSM 710 confocal microscope ...
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bioRxiv - Biochemistry 2020Quote: ... 10 μl samples (in duplicate) were transferred to a 384-well Proxiplate (PerkinElmer, 6007290) before reading on the BMG Labtech PHERAstar FSX at RT using the HTRF 337/520/620 nm module ...
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bioRxiv - Biochemistry 2019Quote: ... 10 mM MgCl2 and 0.1 mM [γ-32P] ATP (∼300–500 c.p.m./pmol, PerkinElmer) in the absence or presence of MLi-2 (1 µM) ...
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bioRxiv - Developmental Biology 2021Quote: ... and 1 µL ATP [γ-32P]- 3000Ci/mmol [10 mCi/mL] (Perkin Elmer NEG002A500UC) were added and incubated for 30 minutes at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 150 µM cold ATP and 10 µCi [γ-32P] ATP (Perkin Elmer) for 30 min at 30°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 1.5 µL UTP [α-32P] (3000 Ci/mmol 10 mCi/ml, 500 µCi, PerkinElmer) was used to replace equivalent unlabeled UTP from the MEGA Script IVT kit ...
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bioRxiv - Biochemistry 2023Quote: ... A mix of 10 uL of 2.5 μg/mL glutathione acceptor beads (PerkinElmer, AL109M) and 5 μg/mL streptavidin donor beads (PerkinElmer ...
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bioRxiv - Cell Biology 2024Quote: ... fluorescent signal was developed by 10 min incubation with TSA-Cy3 solution (Perkin Elmer). Residues were eliminated through stringent 4x SSC washing and slides were prepared for microscope detection using DAPI mounting solution ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 min with horseradish peroxidase-conjugated secondary polymer (anti-mouse/anti-rabbit, Perkin Elmer), and 10 min with horseradish peroxidase-reactive OPAL fluorescent reagents (Perkin Elmer) ...