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Citations for PerkinElmer :
601 - 650 of 685 citations for DnaJ Hsp40 Homolog Subfamily C Member 10 DNAJC10 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Plates sat at room temperature for an additional 10 min in the dark before samples were read on an EnVision XCite plate reader (PerkinElmer # 2105-0020).
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bioRxiv - Microbiology 2019Quote: ... Nluc activity was measured from cell lysates within 10 min of lysis (or substrate addition) by transferring lysate into white OptiPlate 96-well plates (PerkinElmer, Waltham, MA) and measured in a Berthold Centro XS3 LB 960 luminescent plate reader with readings integrated over 0.2 sec.
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bioRxiv - Biochemistry 2021Quote: ... metabolic labeling was started by replacing the medium with phosphate-free DMEM containing 10% dialyzed FBS and 1mCi/mL 32P orthophosphate (PerkinElmer, Waltham, MA). After labeling for 8 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... Mice were injected IP with 10 µL/g of body weight of 15 mg/mL in vivo grade D-Luciferin (luciferin; PerkinElmer, Waltham, MA), anesthetized with 2-3% isoflurane in an induction chamber ...
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bioRxiv - Cell Biology 2023Quote: ... Plates were shaking at 999 rpm and 28°C and growth was measured by monitoring OD600 every 2 h for 10 h using a plate reader (2300 EnSpire™, Perkin Elmer).
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bioRxiv - Physiology 2023Quote: ... was prepared by mixing 40 µL homogenate with an equal volume Nanoluc reaction buffer (PBS: NanoGlo Buffer: NanoGlo substrate furimazine, 30: 10: 0.2) in a black perkin elmer plate (6065400, PerkinElmer, Waltham, MA, USA). Within 2 min of buffer addition ...
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bioRxiv - Microbiology 2023Quote: ... The 3280 compounds were tested in a final concentration of 10 µM with the rhlA-gfp [30] reporter strain in 96 wells microtiter dish (Black Isoplate®, Perkin Elmer). The cells were added in a final concentration of OD600=0.1 and final volume of 200 µl in ABGC medium and incubated at 37°C with shaken (200 rpm) ...
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bioRxiv - Developmental Biology 2024Quote: ... The membranes were washed in TBS-T for 3x 10 minutes and developed with Western Lightning Plus-ECL reagent (Perkin Elmer NEL104). To produce in vitro the Sp5 protein ...
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bioRxiv - Cancer Biology 2024Quote: ... and the plates incubated for 10 min prior to luminescent detection in a Victor 2 microplate reader (Perkin Elmer, Waltham, MA, USA) and the signal was quantified at 595 nm ...
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bioRxiv - Cell Biology 2024Quote: ... followed by 15 µL of 6 M sodium hydroxide for 10 min at room temperature before reading plates at 420 nm on an EnVision plate reader (PerkinElmer; Waltham, MA). Initial velocities for each inhibitory BCKAs concentration were derived by regression fitting of timepoints within distinct linear ranges of each time course ...
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bioRxiv - Biochemistry 2024Quote: ... Fluorescence spectra between 470 to 600 nm at an excitation wavelength of 450 nm (Perkin Elmer LS 55, PM 750, slits 10), were immediately acquired after the dilution and homogenization steps ...
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bioRxiv - Cancer Biology 2020Quote: ... overnight and detected using HRP-conjugated secondary antibodies and chemiluminescence with the Western Lightning Plus-ECL Reagent (Perkin Elmer).
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bioRxiv - Neuroscience 2020Quote: ... HRP conjugated secondary antibodies were used for ECL-mediated visualization (Western Lightening Plus-ECL, #NEL105001EA, Perkin Elmer, Waltham, MA) with film (GeneMate Blue Lite Autorad Film ...
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bioRxiv - Immunology 2022Quote: ... Antibody binding was detected using enhanced chemiluminescent substrates for horseradish peroxidase (HRP) (ECL Western blotting reagents (PerkinElmer, cat: NEL105001EA) or SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... Following TBST washes the membranes were incubated in the relevant secondary antibody: Goat anti-rabbit IgG (Perkin Elmer/ NEF812001EA), diluted (1:10,000 ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were washed again and incubated with the appropriate Opal tertiary antibody (1:100 in amplification diluent, Perkin Elmer) for 60 min ...
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bioRxiv - Developmental Biology 2020Quote: ... incubated 1h in blocking buffer and then incubated overnight with an anti-DNP-POD antibody (1:1000, Perkin Elmer). The next day embryos were washed in PBS-Tween ...
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bioRxiv - Immunology 2024Quote: ... Antibody binding was detected using enhanced chemiluminescent substrates for horseradish peroxidase (HRP) (ECL Western blotting reagents (PerkinElmer, cat: NEL105001EA) or SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... 10 nM full-length 6x-His-tagged human G3BP1 produced in mammalian cells was added to the reaction mix containing test compound and 0.5 nM anti-His-Tb antibody (PerkinElmer). Assays were initiated by adding 4 nM FAM-labeled PEG6-USP10 24-mer peptide probe (FAM-PEG6-GALHSPQYIFGDFSPDEFNQFFVT ...
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bioRxiv - Developmental Biology 2023Quote: ... was used to detect the anti-pH3 antibody after detection of ISH probe by using TSA plus (Perkin Elmer) green (FP1168).
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bioRxiv - Immunology 2024Quote: ... or HIS-SUMO-C-A mutant Rb 380-785 was pre-incubated with 0.4 nM HTRF donor conjugated antibodies anti-GST-Tb or anti-HIS-Tb (PerkinElmer), respectively ...
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bioRxiv - Bioengineering 2021Quote: ... The volume of samples was adjusted to 10 mL by adding ultra-pure water and Gd concentration in each sample was measured using ICP-Mass Spectrometer (PerkinElmer, NexION® 2000). A similar procedure was used for monolayer cell culture (the cells were rinsed with PBS (3x 5 min ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 80-120 mg/kg body weight) / xylazine (Xy, 5-10 mg/kg body weight) cocktail) using an IVIS Spectrum In Vivo Imaging System (Perkin Elmer, Rodgau, Germany) with images acquired using the Living Image® software package (Perkin Elmer).
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bioRxiv - Plant Biology 2021Quote: ... Fluorescence spectra of solutions diluted 10-fold were recorded with 3.5 mL Suprasil® quartz optical cells of 10 mm pathlength using a LS-50B spectrofluorimeter monitored with UVWinlab 5.1 software (Perkin Elmer, Waltham USA). For each compound ...
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bioRxiv - Plant Biology 2020Quote: ... detection limit 5 ng.L−1) and K+ content by ICP-AES (-Atomic Emission Spectrometry, OPTIMA 8300, Perkin Elmer, quantification limit 10 μg.L−1).
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bioRxiv - Cancer Biology 2022Quote: ... Organoid/hydrogel cultures were serum starved overnight before treatment with EGF (20 ng/mL) and promegestone/R5020 (1, 10, 100 nM; Perkin Elmer, Cat. #: NLP00400) and 10 nM R5020 with or without the addition of FAK (1 μM ...
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bioRxiv - Plant Biology 2019Quote: ... 10mm root tip from 10 seedlings were collected for each sample and placed into the scintillation vial with 500µL MicroScint 40 (Perkin Elmer, Inc., USA, www.perkinelmer.com). The uptake of 137Cs+ was determined with a NaI scintillation counter (ARC-300 ...
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bioRxiv - Genomics 2020Quote: Monocytes from three unrelated healthy participants were plated in 30 µL of RPMI 1640 and 10% FBS on CellCarrier-384 well Ultra Microplates coated with PDL (PerkinElmer; cat. no. 6057500) at a concentration of 100,000 cells/well ...
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bioRxiv - Microbiology 2020Quote: ... 20 μCi of radioactive Pi solution (10 μL from a 2 mCi K2H32PO4 at a concentration of 2 mM of K2H32PO4, PerkinElmer cat. no. NEX055) was added to 1 mL of cell suspension ...
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bioRxiv - Genetics 2019Quote: ... evaporated under nitrogen gas (Organomation Associates, Inc., Berlin, MA, USA) and resuspended in 10 mL Insta-Gel Plus scintillation fluid (Perkin Elmer, Cat#6013399), followed by counting with a Beckman LS6500 scintillation counter ...
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bioRxiv - Genomics 2024Quote: ... size-selected sample was reamplified with 25 μL of NEXTFLEX PCR Master Mix 2.0 and 2 μL of 1:10 diluted NEXTFLEX Primer Mix 2.0 as described in the NEXTFLEX Cell free DNA-Seq Kit (V2) (Perkin Elmer, Pittsburgh, Pennsylvania, USA), step C PCR amplification ...
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bioRxiv - Cancer Biology 2023Quote: ... was injected intraperitoneally at a dose of 150 μg/g and incubated for 10 min before scanning in the IVIS® Spectrum In Vivo Imaging System (PerkinElmer, Waltham, MS).
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... 5-HT uptake was initiated by the addition of 10 nM [3H] 5-HT (Cat No: NET498001MC; 24.82 Ci/mmol: Perkin Elmer, Santa Clara, CA). and the uptake was terminated at 5 min with the addition of fluoxetine followed by rapid filtration over GF-B filters ...
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bioRxiv - Biochemistry 2024Quote: ... The lysed cells were diluted 1:2 with assay buffer after which 10 μL/well was pipetted to a white 384-well OptiPlate (PerkinElmer, Waltham, MA, USA). Next ...
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bioRxiv - Immunology 2021Quote: ... Immunoreactive proteins were detected with either anti-mouse or anti-rabbit horseradish peroxidase-coupled secondary antibody followed by detection with enhanced chemiluminescence (PerkinElmer).
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bioRxiv - Neuroscience 2021Quote: ... Molecular weight bands were visualized by incubation with peroxidase-conjugated anti-mouse or anti-rabbit secondary antibody (1:5000; Jackson Immuno Research Laboratories) followed by treatment with the ECL-plus reagent (Western Lighting; PerkinElmer) and exposure on G:Box Chemi XRQ system (Syngene) ...
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bioRxiv - Neuroscience 2019Quote: ... Primary antibodies were detected by incubation with peroxidase-conjugated anti-mouse and anti-rabbit antibodies (1:10000, Jackson Immuno Research Laboratories) followed by reaction with ECL-Plus reagent (Western Lighting, PerkinElmer) and exposure to autoradiography films ...
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bioRxiv - Neuroscience 2021Quote: ... and Lumi4-Tb cryptate conjugated anti-IP1 antibody (emission at 620 nm) was detected using an Envision plate reader (PerkinElmer). Results were calculated from the 665/620 nm ratio and normalised to the maximum response stimulated by acetylcholine.
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bioRxiv - Neuroscience 2022Quote: ... membranes and immunoblots were carried out using primary antibodies (Table S4) followed by incubation with appropriate secondary horseradish peroxidase-conjugated antibodies (Table S5) and chemoluminiscent detection (Western Lightning, PerkinElmer). All antibodies were diluted in PBS containing 5% semi-skimmed milk and 0.1% Tween-20 ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were washed and incubated with the goat anti-rabbit IgG HRP-labeled secondary antibody (PerkinElmer, Waltham, MA, USA; NEF812001EA) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... anti-mouse or anti-rabbit secondary antibody (1:10000; Jackson Immuno Research Laboratories) followed by treatment with ECL-plus reagent (Western Lighting, PerkinElmer) and exposure to autoradiography films ...
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bioRxiv - Developmental Biology 2021Quote: ... Antisense RNA probes labeled with DNP were used in all in situ hybridizations and probe detection was performed using a HRP-coupled anti-DNP antibody (PerkinElmer) and a fluorescein coupled tyramide (Vize et al. ...
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bioRxiv - Microbiology 2022Quote: ... The membranes were then incubated with anti-IgG rabbit antibodies conjugated to horseradish peroxidase (PerkinElmer Life Sciences (Boston, MA, USA), and developed using the Western Lightning Chemiluminescence Reagent Plus (PerkinElmer) ...
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bioRxiv - Neuroscience 2022Quote: ... then incubated for 30 min at 25 °C with shaking at 400 RPM with 9 ng/well of DELFIA Eu-N1 anti-mouse antibody in DELFIA assay buffer (PerkinElmer), washed with 3 × 100 μl of PBST before enhancing with 80 μl of DELFIA Enhancement Solution (PerkinElmer) ...
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bioRxiv - Biochemistry 2022Quote: ... 5 μL time points were taken and quenched with 1.33 mM EDTA then brought to 20 μL final volume for detection using 1 nM LANCE Ultra Europium anti-H3K4me1/2 antibody (TRF0402, PerkinElmer) and 50 nM LANCE Ultra Ulight-Streptavidin (TRF0102 ...
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bioRxiv - Immunology 2022Quote: ... Virus–monoclonal antibody-containing medium was then aspirated from cells and 100Lµl of a 1:2 dilution of SteadyLite Plus (PerkinElmer, 6066759) in PBS with Ca2+ and Mg2+ was added to cells ...
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bioRxiv - Microbiology 2024Quote: ... Blots were probed with the indicated antibodies and developed using the Western Lighting plus ECL (Perkin Elmer, Waltham, MA, USA). Blots were imaged on the Bio-Rad ChemiDoc MP Imaging System (BioRad ...
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bioRxiv - Biochemistry 2023Quote: ... FLAG and HA were detected using the LI-COR system and HRP-conjugated GAPDH primary antibody was incubated with blots for 2 hours at room temperature and detected with Western Lightning Plus-ECL (PerkinElmer) using a Bio-Rad Chemidoc System.
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bioRxiv - Developmental Biology 2024Quote: ... followed by secondary antibody incubation for 30 min at room temperature with HRP conjugated Goat anti Rabbit (Perkin Elmer, NEF812001EA). The slides were then developed with Opal 780 fluor at 1:25 (Akoya ...
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bioRxiv - Biochemistry 2021Quote: ... and 10 μL of 1/5000 dilution of Eu-W8044 labeled streptavidin and Biotin-cAMP in the Detection Buffer (PerkinElmer Life and Analytical Sciences) were added in reduced lighting conditions ...