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Citations for Worthington Biochemical :
401 - 428 of 428 citations for HLA A*0201 NY ESO 1 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... followed by a 10× dilution using 100 mM Tris-HCl (pH = 8.0) and tryptic digestion under 37°C for 18 hours using 1 µg of trypsin per well (Worthington Biochemical Corp., Lakewood, NJ). Digested peptides were desalted using a panel of lab-made 96-channel filter tips generated by inserting 96 20 µl filter tips into a 96-well cover mat and stacking each filter tip with 5 mg of 10-μm C18 column beads ...
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bioRxiv - Cancer Biology 2020Quote: ... Homogenised tissue was added to a solution (total volume of 7 ml) composed by of 1 mg/ml collagenase IV (Worthington Biochemical Corp, USA), 0.02 mg/ml DNase 1 (New England Biolabs ...
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bioRxiv - Cell Biology 2021Quote: ... A single cell suspension of vascular stromal cells was isolated from minced iBAT (using scissors) digested for 90 min with collagenase IV (3 mg mL-1, Worthington, Lakewood NJ, USA) at 37 °C under gentle agitation16 ...
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bioRxiv - Microbiology 2022Quote: ... of 0.01 and maintained in MEM containing 0.3% bovine serum albumin (MEM/BSA) and 1 μg/mL of L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK)-trypsin (Worthington, Lakewood, NJ, USA). Supernatants were collected every 12 h and viral titers were measured using a plaque assay.
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bioRxiv - Immunology 2021Quote: ... Collected lobes were incubated for one hour at 37°C in 10 mL of cRPMI with 1500 units of 260 units/mg type 1 collagenase (Worthington Biochemical, Cat. #LS004194) per lobe and pressed through a 35 µm filter ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004194, 100 mg, $42.00 Ask -
bioRxiv - Microbiology 2021Quote: ... followed by 10X dilution into 50 mM ABC (pH 8.0) and tryptic digestion at 37°C for 20 hr with shaking using 1 μg of trypsin (Worthington Biochemical Corp., Lakewood, NJ) per 50 μg protein ...
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bioRxiv - Cancer Biology 2022Quote: ... The obtained liver tissues were homogenized in 8 mL of RPMI1640 media containing 1.8 mg/ml Collagenase IV (WOR-CLS4-1, Worthington Biochemical Corporation, NJ, USA) and 112.3 µg/ml DNase I (11284932001 ...
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bioRxiv - Developmental Biology 2023Quote: ... Tissue fragments were incubated in 1.35 mL PBS plus 150 uL 20 mg/mL Collagenase Type I (Worthington CLS-1 dissolved in PBS) and 15 uL 20 mg/ml DNase I (Worthington D dissolved in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... spun down by centrifugation at 500 x g for 5 minutes and resuspended in 10 ml of digestion medium (MEM containing 112 U/mL type I collagenase; Worthington, cat. #CLS-1), 1.2 U/mL dispase (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... Brain tissues were manually minced using razor blades and enzymatically dissociated at 37°C for 30 min with 1 mg/mL collagenase type IV (#LS004188, Worthington Biochemical Corporation, USA) and 250 U/mL DNase 1 (#10104159001 ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Paleontology 2023Quote: ... the OBM sections were then incubated overnight at 37 degrees Celsius in 1 mg/mL collagenase (collagenase type I, Worthington Biochemical Corporation, 55B7870) with 1% sodium azide dissolved in Dulbecco’s PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Human brain tissue was manually minced using razor blades and enzymatically dissociated at 37°C for 30 minutes with 1 mg/ml collagenase-4 (#LS004188, Worthington Biochemical Corporation, USA) and 250 U/ml DNAse1 (#10104159001 ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004188, 1 gm, $215.00 Ask -
bioRxiv - Neuroscience 2023Quote: Retinas from 2 injected mice for each of the different Cy3-labelled siRNAs were pooled and dissociated into single cells using papain for dissociation according to the manufacture’s instructions (Worthington; Cat# 9035-81-1). Each conjugate was done in triplicates with a total of 6 mice per configuration ...
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bioRxiv - Paleontology 2023Quote: ... The matrix sections were then incubated overnight at 37 degrees Celsius in a solution of ∼1 mg/mL collagenase (collagenase type I, Worthington Biochemical Corporation, 55B7870) dissolved in Dulbecco’s PBS (pH 7.2 ...
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bioRxiv - Biochemistry 2024Quote: ... we processed approximately 1 g of adipose tissue by mincing and enzymatic dissociation in 0.1% (w/v) collagenase type II (Worthington Biochemical Corporation, Lakewood, NJ) at 37°C for 2 hours with gentle shaking ...
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bioRxiv - Cell Biology 2019Quote: ... muscle of 6-week old male C57 BL/7 mice was dissected out and treated with Collagenase Type 1 (Cat# LS4196 Worthington 400U/ml final concentration) for 1 hour at 37°C ...
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bioRxiv - Physiology 2020Quote: ... oocytes contained in 1-3 mL of ovary lobes extracted via survival surgery were isolated by enzymatic digestion with collagenase type II (Worthington Biochemical Corp., NJ, USA) under mechanical agitation ...
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bioRxiv - Molecular Biology 2022Quote: Calvarial osteoblasts were obtained from 3 to 5-day-old C57BL/6 mice by sequential enzyme digestion [1 mg/ml collagenase type II (Worthington Biochemical, Lakewood, NJ, USA) in Hanks’ balanced salt solution (Life Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... Cortices were isolated from postnatal day 0 (P0) CD-1 or cGKII KO mouse brain tissues and digested with 10 U/mL papain (Worthington Biochemical Corp. Lakewood, NJ). Mouse cortical neurons were plated on following poly lysine-coated dishes for each experiment - 24 wells (200,000 cells ...
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bioRxiv - Microbiology 2022Quote: Enzymatic digestion of lung tissue was performed by intratracheal instillation via a 20-gauge catheter of 1 mL of 5 mg/mL collagenase I (Worthington Biochemical Corp, Lakewood, NJ) and 0.25 mg/mL DNase I (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were removed and the lateral SVZs were dissected in cold DMEM-F12-glutamax 1/50 Glc 45% and subsequently incubated in digestion medium (papain [Worthington], DNase [Worthington], L-cysteine [Sigma-Aldrich]) for 30 min at 37 °C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and 24 h post-transfection the media was supplemented with 1 μg/mL of tosylsulfonyl phenylalanyl chloromethyl ketone (TPCK)-treated trypsin (Worthington Biochemicals, Lakewood, NJ, USA). The rgH9N1 viruses were propagated on MDCK cells and harvested 72 hours post-inoculations ...
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bioRxiv - Immunology 2023Quote: ... Virus-infected cells were grown in MEM-BSA-TPCK media[20] that was prepared similar to the MEM media described above but supplemented with 1 µg/mL Tosyl phenylalanyl chloromethyl ketone (TPCK) trypsin (Worthington Chemical Corporation, Lakewood, NJ) and with Bovine Albumin Fraction V (7.5% solution ...
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bioRxiv - Neuroscience 2023Quote: Both retinas of three Csf1rGFP mice were dissected at different timepoints (Day 1, 3, and 7) and incubated with papain (Worthington Biochemical, Freehold, NJ, USA) for 15 min as previously described (26) ...
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bioRxiv - Neuroscience 2023Quote: ... and 1 mM Na-pyruvate) and dissociated them by adding an equal volume of 2 x Papain stock solution (Worthington Biochemical Corporation, Lakewood, USA) at 37°C for 20 minutes ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Lungs were digested by intratracheal instillation via a 20-gauge catheter of 1 mL of 5 mg/mL collagenase I (Worthington Biochemical Corp, Lakewood, NJ) and 0.25 mg/mL DNase I (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... the digestion was stopped by a solution of trypsin inhibitors (Leibovitz’s L15 medium, 10% 1 mg/ml trypsin inhibitor [Worthington], and 2% 10 mg/ml DNAse) and cells were washed with L15 medium ...
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bioRxiv - Bioengineering 2022Quote: ... and then inflated via the trachea with 2-3 mL of pre-warmed (37°C) enzyme solution (1 mg/mL collagenase/dispase [Roche], 3 U/mL elastase [Worthington], 20 U/mL DNase [Worthington] in DMEM). The heart and large airways were dissected away and the remaining lung tissues and enzyme solution were collected and transferred to a shaking 37°C water bath at 75 rpm for 20 min ...