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Citations for Worthington Biochemical :
151 - 200 of 524 citations for Trypsin 1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... Cell suspensions were harvested by mechanical dissection and Collagenase IV Cocktail digestion (formulated as 7.4 kU Collagenase Type 4, 53 kU Deoxyribonuclease I, 20 mg Soybean Trypsin Inhibitor in 10 mL cocktail; Worthington Biochemical). The suspensions were passed through 40 μm cell strainers to ensure singularity ...
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bioRxiv - Plant Biology 2021Quote: ... Samples were incubated at 37°C for one hour followed by adding 1.5 μg/mL trypsin inhibitor (Worthington Biochemical. Corp) to inactivate trypsin ...
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bioRxiv - Cell Biology 2022Quote: ... The tissue pieces were dissociated in phosphate-buffered saline (PBS) containing 0.25% [w/v] trypsin (Worthington Biochemical Corp., Lakewood, NJ), 0.1% [w/v] DNase-I (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... The ventricles were cut into 1-2mm pieces and the cardiac myocytes isolation was achieved by digestion with 0.04% trypsin and type II collagenase (Worthington #LS004176) 0.4mg/ml in KH buffer at 37°C ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Cell Biology 2020Quote: ... were generated using the eight plasmid-based systems as previously described (Wit et al., 2004) and propagated on MDCK-II cells in presence of TPCK-trypsin (Worthington). For infection ...
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bioRxiv - Microbiology 2020Quote: ... 1 hour after infection media was changed to serum-free media supplemented with 1µg/ml tosyl phenylalanyl chloromethyl ketone (TPCK)-treated trypsin (Worthington-Biochemical). Passage experiments were performed by infecting cells at an MOI of 0.01 ...
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bioRxiv - Developmental Biology 2022Quote: ... The individually isolated spinal cords were digested at 37°C for 8 min in 0.25 mg/ml of trypsin (Worthington Biochemical), and the digestion stopped with 10x trypsin-inhibitor (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... Tissues were then incubated at 37°C for 3 minutes in a solution of HBSS/0.2% glucose with 10 mg/ml trypsin (Worthington Biochemical) and 1 mg/ml Deoxyribonuclease I (Worthington Biochemical) ...
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bioRxiv - Molecular Biology 2020Quote: ... the proteins were precipitated by trichloroacetic acid (TCA) (PAGE Clean Up Kit, Nacalai Tesque) and then digested with trypsin (TPCK-treated, Worthington Biochemical). The digestion mixture was separated on a nanoflow LC (Easy nLC 1200 ...
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bioRxiv - Neuroscience 2019Quote: ... following genotyping or embryonic day 18 embryos (rat) and dissociated enzymatically for 20 min at 37 °C in HBSS supplemented with 0.25% (w/v) trypsin (Worthington Cat# LS003707), followed by mechanical dissociation via trituration with fire-polished glass Pasteur pipettes ...
Product cited:
Supplied as a chromatographically purified, diafiltered and lyophilized powder.Cat# LS003707, 1 gm, $325.00 Ask -
bioRxiv - Immunology 2019Quote: ... and the GL261 tumors were excised and dissociated in Collagenase IV Cocktail (3.2 mg/mL Collagenase Type 4, 1.0 mg/mL deoxyribonuclease I, 2 mg/mL Soybean Trypsin Inhibitor [Worthington Biochemical, Lakewood, NJ]) for two 15 min intervals with gentle mixing at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... which included a first step of PDX tumor mechanical dissociation with sterile scalpels and then enzymatic dissociation by trypsin (10 mg/ml) and type II collagenase (275 U/mg, Worthington Biochemical). The tube was then placed in a warm 37°C water bath for 60 min ...
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bioRxiv - Biophysics 2021Quote: ... this media is similar to the MEM media described above but supplemented with Tosyl phenylalanyl chloromethyl ketone (TPCK) trypsin (Worthington Chemical Corporation) and with Bovine Albumin Fraction V (7.5% solution ...
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bioRxiv - Immunology 2021Quote: ... Cells were infected with lung homogenate supernatants at varying dilutions for 45 min at 37°C before the addition of Leibovitz L15 or MEM medium containing 0.9% agarose overlay containing Trypsin (Worthington Biochemical, NJ, USA), as previously described[54] ...
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bioRxiv - Neuroscience 2023Quote: ... The digestion solution is then aspirated off and the tissue is resuspended in a 2 mg/ml trypsin inhibitor ovomucoid solution (Lo Ovo) (Worthington, Cat# LS003083). Cells were then spun in a centrifuge at 300g for 11 minutes at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... using 300μL/heart of ADS buffer containing 0.1% Trypsin (SigmaAldrich, Cat. No. T4799) and 0.002% DNase (Worthington Biochemical Corp., Cat. No LS002006). Each digestion lasted 10-20 minutes ...
Product cited:
Chromatographically purified. A lyophilized powder with glycine as a stabilizer.Cat# LS002006, 20 mg, $96.00 Ask -
bioRxiv - Microbiology 2022Quote: ... with 10% fetal calf serum and antibiotics/antimycotics supplemented with 1g/ml tosyl phenylalanyl chloromethyl ketone (TPCK)-trypsin (Worthington Biochemical Corp., Lakewood, NJ).
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bioRxiv - Microbiology 2024Quote: ... The viruses were propagated in MDCK cells in the presence of L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK)-treated trypsin (Worthington Biochemical Corporation, Lakewood, NJ).
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bioRxiv - Systems Biology 2019Quote: ... then the samples were diluted by ten folds with 100 mM ammonium bicarbonate buffer followed by digestion using lysyl endopeptidase (Lys-C; Wako Pure Chemical Corp., Osaka, Japan) for 4 hours and trypsin (Worthington Biochemical Corp., Lakewood, NJ, USA) for overnight at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 10~15 mg of proteins were then used for a sequential digestion using lysyl endopeptidase (Lys-C; Wako Pure Chemical Corp., Osaka, Japan) and trypsin (Worthington Biochemical Corp., Lakewood, NJ, USA). Briefly ...
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bioRxiv - Neuroscience 2023Quote: ... Dissociation medium was aspirated until 1 mL remained and an activated papain solution (1:1 papain [Worthington-Biochem] with 13.6 mM Cysteine-HCL ...
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bioRxiv - Developmental Biology 2023Quote: ... in 1:1 DMEM:F12K supplemented with DNase I (Worthington Biochemical), 6.5mg/ml collagenase (Worthington Biochemical) ...
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bioRxiv - Neuroscience 2020Quote: ... prepared using 1:1 albumin-ovomucoid solution (10mg/ml of each) (Worthington, Cat# OI ...
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bioRxiv - Genomics 2023Quote: ... for 1 hour (Patient #1) or 3.7mg/mL collagenase II (Worthington, LS004177) for 90 minutes followed by 6mg/mL dispase (Gibco ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004177, 5 gm, $900.00 Ask -
bioRxiv - Cancer Biology 2024Quote: ... containing 1 mg/ml each of Collagenase Type 1 (Worthington Biochemical LS004216), Type 2 (Worthington Biochemical LS004204) ...
Product cited:
Collagenase, Type 1 (Code: CLS-1), which is filtered through a 0.22 micron membrane and...Cat# LS004216, 5x50 mg, $200.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... and 1% DNase (Worthington) at 37°C for 6 minutes at which point DMEM (Gibco ...
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bioRxiv - Biochemistry 2024Quote: ... Lungs from 4 mice were minced into ∼1 mm pieces using curved scissors and digested with 1 mg/ml collagenase type 1 (Worthington, cat# LS004194) and 1X dispase (Corning ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004194, 100 mg, $42.00 Ask -
bioRxiv - Neuroscience 2020Quote: ... papain (1 mg/ml, Worthington), ethylenediaminetetraacetic acid (EDTA ...
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bioRxiv - Immunology 2023Quote: ... 1 mg/ml hyaluronidase (Worthington), 0.1 mg/ml DNase I (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... This solution was then replaced with 1mg/ml collagenase 1 (Worthington Biochemical Corporation CLS-1) in 0.1M PBS and 20mM HEPES for 60-90 min at 37°C followed by 0.05% Trypsin-EDTA (Sigma T9201 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were spun down and supernatant was removed before the addition of 1 ml Dispase-mUE (Dispase II [7 mg ml-1 DMEM Ham’s F12], Neutral Protease, Worthington NPRO2; and 10 μg μL-1 DNase). Cells were aspirated in the solution gently 40 times using a 1 ml pipette tip ...
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bioRxiv - Physiology 2019Quote: ... 2.5mg/ml collagenase Type 1 (Worthington) made up in low Ca2+/low Mg2+ HBSS ...
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bioRxiv - Immunology 2022Quote: ... dispase (0.1 U ml−1; Worthington) and 10% FBS ...
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bioRxiv - Physiology 2022Quote: ... 2.5mg/ml collagenase Type 1 (Worthington) made up in low Ca2+/low Mg2+ HBSS ...
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bioRxiv - Microbiology 2021Quote: ... 30 mg ml-1 DNase (Worthington), and 1 mM MgCl2 ...
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bioRxiv - Cancer Biology 2023Quote: ... and Deoxyribonuclease 1 (Cat: LS002139, Worthington). Next ...
Product cited:
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bioRxiv - Neuroscience 2022Quote: ... and 1:1000 DNase (Worthington Biochemical). 0.02% TritonX-100 (v/v ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 U/mL of dispase (Worthington) and 5uM of Ca2+(Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2020Quote: ... with Papain added to 1 mg/ml (1:10 dilution of 10 mg/ml Papain solution; Worthington)) ...
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bioRxiv - Neuroscience 2021Quote: ... was added to halt the proteolysis and samples were centrifuged at 300xG for 10 minutes at 4°C and resuspended in a 1:1:10 solution of DNase:ovomucoid:Neurobasal media (Worthington:Worthington:Gibco). Cell suspensions were incubated with primary antibodies to CD11b and CD45 (table 1 ...
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bioRxiv - Neuroscience 2021Quote: ... was added to halt the proteolysis and samples were centrifuged at 300xG for 10 minutes at 4°C and resuspended in a 1:1:10 solution of DNase:ovomucoid:Neurobasal media (Worthington:Worthington:Gibco). Cell suspensions were incubated with primary antibodies to CD11b and CD45 (table 1 ...
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bioRxiv - Genetics 2019Quote: ... + 250 U/ml Collagenase Type 1 (Worthington) + 50 µg/ml DNAse I (Sigma)) ...
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bioRxiv - Immunology 2019Quote: ... 1 mg/ml Type II collagenase (Worthington), and 50 U/ml Deoxyribonuclease I (Worthington ...
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bioRxiv - Cancer Biology 2019Quote: ... 1 ml collagenase IV (Worthington Biochemical Corp.), and 5 mM CaCl2) ...
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bioRxiv - Immunology 2019Quote: ... 1 mg/ml Type II collagenase (Worthington), and 50 U/ml Deoxyribonuclease I (Worthington ...
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bioRxiv - Immunology 2022Quote: ... 1 mg/ml Type II collagenase (Worthington), and 50 U/ml Deoxyribonuclease I (Worthington ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mg/mL type 2 collagenase (Worthington), 10 µg/mL gentamycin (Teknova ...
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bioRxiv - Microbiology 2020Quote: ... 1 mg/mL chymotrypsin (Worthington Biochemical Corporation), 1 mg/mL trypsin (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... 1 mg/mL Dispase (Worthington Biochemical, LS02104) in L-15 media for 20 minutes at 37°C with gentle agitation every 5 minutes ...
Product cited:
Animal Free, Chromatographically purified. A lyophilized powder.Cat# LS02104, 50 mg, $352.00 Ask -
bioRxiv - Developmental Biology 2021Quote: ... 1:10000 DNase-I (Worthington Biochemical, LS006342), before filtering through a 70-μm strainer (Falcon ...
Product cited:
Molecular Biology Grade. Chromatographically purified to remove RNase and protease. Supplied...Cat# LS006342, 100 un, $32.00 Ask