Labshake search
Citations for Worthington Biochemical :
51 - 100 of 104 citations for 3 3 Bromomethyl phenyl thiophene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... Minced heart tissue was digested in 3 mL of digestion buffer (2 mg/mL Collagenase IV [Worthington Biochemical] ...
-
bioRxiv - Cancer Biology 2023Quote: ... minced and digested in 5 mL of Advanced DMEM/F12 containing 10 mg Collagenase Type 3 (Worthington) and 500 U Hyaluronidase (Sigma ...
-
bioRxiv - Cell Biology 2021Quote: ... HBSS was discarded and stromal cells were released by digestion with 3 mg/ml Type I Collagenase (Worthington) in 1 ml HBSS for 10 min twice at 37°C with shaking at 110 rpm ...
-
bioRxiv - Developmental Biology 2022Quote: ... the mouse knee was minced into small fragments and digested with 3 mg/ml collagenase type I (Worthington) and 4 mg/ml dispase (Roche ...
-
The cellular basis of protease activated receptor type 2 (PAR2) evoked mechanical and affective painbioRxiv - Neuroscience 2020Quote: ... followed by 25 minutes of incubation at 37□C in 3 mg/ml collagenase type 2 (LS004176; Worthington) and 2 mg/ml Dispase II (04942078001 ...
-
bioRxiv - Bioengineering 2022Quote: ... vessels were removed from the perfused system and incubated in 3 Kunitz/ml protease-free DNAse (Worthington, LS006343) in 1x enzyme reaction buffer (40 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Physiology 2024Quote: ... cut into small fragments and dissociated in 2 mL HBSS containing 3 mg/mL collagenase type II (Worthington), 7.5mg/mL dispase II and 0.25 mg/mL DNase I for 30 minutes at 37°C ...
-
bioRxiv - Cell Biology 2024Quote: ... The plugs were digested with 1 ml of a solution of 3 mg/ml type I collagenase (Worthington) dissolved in HBSS for 10 min at 37 °C with 110 rpm shaking ...
-
bioRxiv - Developmental Biology 2021Quote: ... 3 sutures each from litter 2) were cut into small fragments in HBBS and digested using Collagenase IV (Worthington, USA ...
-
bioRxiv - Neuroscience 2021Quote: ... Thermo Fisher, 31966-021 with 3.6 mg/ml glucose, Carl Roth, NH06.3, 3 mg/ml Collagenase type IV, Worthington, LS004186 ...
-
bioRxiv - Neuroscience 2021Quote: Differentiated human neural cultures (3 months old) were dissociated into single-cells suspension using Papain Dissociation System (Worthington #LK003150), passed through 70µm and 40µm cell strainers (Falcon #07-201-431 and #07-201-430) ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Bioengineering 2021Quote: ... The solution was replaced with 200 μl trehalose-HEPES-ACSF containing 3 mg/ml ovomucoid inhibitor (OI-BSA, Worthington) and 1 μl DNase I ...
-
bioRxiv - Neuroscience 2020Quote: ... and resuspended in 3 ml HA –Ca containing 10% v/v ovomucoid (Worthington, resuspended in 32 ml HA –Ca). Cell debris was removed using discontinuous density gradient containing 3 ml tissue mixture on top of 5 ml ovomucoid solution ...
-
bioRxiv - Neuroscience 2021Quote: ... Dissected brains were chilled on ice and minced in digestion media containing with 0.2% collagenase type 3 (LS004182, Worthington) and 3 U/mL dispase (LS02104 ...
Product cited:
Lower in secondary proteolytic contaminant activities but with typical collagenase activity. ...Cat# LS004182, 1 gm, $215.00 Ask -
bioRxiv - Developmental Biology 2022Quote: ... MM from E11.5 kidneys from CD-1 mice were dissociated with Collagenase Type 3 (Worthington Biochemical Corporation; Cat #LS004180) and DNase I (New England Biolabs ...
-
bioRxiv - Developmental Biology 2022Quote: ... and digested with gentle rocking for 1 h at 37°C in 3 mg/mL collagenase II (Worthington, LS004176) diluted in HBSS containing 5% fetal bovine serum (FBS) ...
-
bioRxiv - Immunology 2020Quote: ... and skin was minced finely with dissection scissors and mixed in a 6-well plate with 3 ml of digestion buffer consisting of 0.8 mg/ml Collagenase Type 4 (4188; Worthington), 0.02 mg/ml DNAse (DN25-1G ...
-
bioRxiv - Neuroscience 2024Quote: ... followed by incubation at 37° C for 25 minutes in 3 mg/mL collagenase type 2 (LS004174, Worthington Biochemical) and 2 mg/mL Dispase II (4942078001 ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004174, 100 mg, $42.00 Ask -
bioRxiv - Cancer Biology 2023Quote: ... The minced tumors were digested with 5ml to 10ml of 1 mg/ml Collagenase 3 (Worthington cat. no. LS004183) plus 0.5mg/ml Hyaluronidase (Sigma-Aldrich H3884 ...
Product cited:
Lower in secondary proteolytic contaminant activities but with typical collagenase activity. ...Cat# LS004183, 5 gm, $900.00 Ask -
bioRxiv - Microbiology 2022Quote: ... lung tissue was minced in 5 ml of 1 x PBS containing 3 mg/ml collagenase type IV (Worthington). Samples were incubated at 37°C for 45 min and washed with 1 x PBS three times ...
-
bioRxiv - Cancer Biology 2023Quote: ... and spleen (n = 3; 12-16 PCW) samples were diced and digested with 1.6mg/mL collagenase type IV (Worthington) in RPMI (Sigma-Aldrich ...
-
bioRxiv - Bioengineering 2024Quote: We extracted cells from the central body of freshly dissected menisci from 3-week-old WT and Col5a1+/− mice by 0.2% collagenase (CLS-2, Worthington) digestion for 5 h at 37°C ...
-
bioRxiv - Cell Biology 2024Quote: ... the tendons were minced to 1mm3 pieces and digested with 3 mg/ml collagenase I (Worthington biochemical, cat# LS004194) in DMEM (Gibco ...
-
bioRxiv - Neuroscience 2021Quote: ... permeabilized cells were incubated with DNAse I recombinase (3-3000 U/ml in 50 mM Tris-HCL, pH 7.5, 1/ mg/ml BSA: Worthington, LK003170) prior to incubation with TUNEL mixture.
Product cited:
A component of the Papain Dissociation System. This material is 0.22 micron membrane filtered...Cat# LK003170, 1 vi, $28.00 Ask -
bioRxiv - Cancer Biology 2020Quote: Spleens were collected into RPMI 1640 containing 2% fetal calf serum (FCS) and dissociated with a scalpel and type 3 collagenase (Worthington) and DNAse I ...
-
bioRxiv - Cell Biology 2020Quote: Organoid cultures within 2-3 weeks of primary plating were dissociated with 1 U/ml neutral protease (Worthington, Cat LS02100) and 100 KU of DNase I in organoid media ...
Product cited:
Animal Free, Chromatographically purified. A lyophilized powder.Cat# LS02100, 10 mg, $84.00 Ask -
bioRxiv - Developmental Biology 2021Quote: ... and 10 pooled coronal sutures were rinsed in PBS and enzymatically dissociated with a final concentration of 3 mg/mL of Collagenase II (Worthington) and 4 units/mL of Dispase (Corning ...
-
bioRxiv - Neuroscience 2022Quote: ... Tissues were then incubated at 37°C for 3 minutes in a solution of HBSS/0.2% glucose with 10 mg/ml trypsin (Worthington Biochemical) and 1 mg/ml Deoxyribonuclease I (Worthington Biochemical) ...
-
bioRxiv - Genomics 2024Quote: ... we replaced the medium with 2 mL of digestive enzyme mixture I (3 mg/mL collagenase I (Worthington, cat. LS004196), 3 mg/mL collagenase II (Worthington ...
Product cited:
The original balance of enzymatic activities. Each lot assayed for collagenase, caseinase,...Cat# LS004196, 1 gm, $215.00 Ask -
bioRxiv - Immunology 2024Quote: ... Spleens and non-draining lymph nodes were digested in 3 mL of 1.6 mg/mL collagenase Type IV (Worthington Biochemical), 0.1 mg/mL DNase I (Thomas Scientific ...
-
bioRxiv - Immunology 2023Quote: ... A 2 cm2 patch of skin was minced and incubated in 3 mg/mL collagenase Type IV (Worthington Biochemical Corporation) and 2 mg/mL DNase (Sigma-Aldrich ...
-
bioRxiv - Genetics 2022Quote: ... cortices and hippocampi acquired from 12 male mice from 3 separate litters were combined and dissociated using activated 20 U/mL Papain/DNase (Worthington) diluted in Hibernate A (Gibco ...
-
bioRxiv - Neuroscience 2024Quote: ... 3 mM CNQX) and single cell suspensions were acquired with an entire mouse cortex using a papain dissociation kit (Worthington Biochemical ...
-
bioRxiv - Neuroscience 2024Quote: ... samples were pre-cut with small scissors and diluted and digested in 2:3 stock solution of CollagenaseI (10 U/ml, Worthington), Collagenase IV (400 U/ml ...
-
bioRxiv - Microbiology 2022Quote: ... of 0.01 and maintained in MEM containing 0.3% bovine serum albumin (MEM/BSA) and 1 μg/mL of L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK)-trypsin (Worthington, Lakewood, NJ, USA). Supernatants were collected every 12 h and viral titers were measured using a plaque assay.
-
bioRxiv - Cell Biology 2022Quote: ... and the telencephalon was manually cut into pieces, followed by enzymatic digestion (DMEM GlutaMAX 1% P/S, 3% papain (Worthington 3126), 1.5% 10 mg ml−1 DNAse and 2.4% 12 mg ml−1 cystein ...
-
bioRxiv - Immunology 2020Quote: ... inflated with a 1.5 ml mixture of 1 ml low melting agarose (3% w/v) and 500 μl 4U/ml dispase (Worthington Biochem, LS02104), and incubated for 1 h at room temperature (RT) ...
Product cited:
Animal Free, Chromatographically purified. A lyophilized powder.Cat# LS02104, 50 mg, $352.00 Ask -
bioRxiv - Molecular Biology 2020Quote: ... enzymatic digestion was achieved by 3 min of perfusion with calcium-free digestion buffer (400 units/mL of collagenase II in perfusion buffer; Worthington LS004177) followed by 12 min of perfusion with digestion buffer containing 50 μM CaCl2 ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004177, 5 gm, $900.00 Ask -
bioRxiv - Biophysics 2022Quote: ... CaCl2 was added to a final concentration of 40 mM (21 µL of 1 M) followed by the addition of 75 U (3 µL of 25 U/µL) of micrococcal nuclease (MNase, Worthington Biochemical) and incubated at 37◦C for 5 min ...
-
bioRxiv - Cell Biology 2023Quote: ... The tissue was then washed with PBS and digested for 10 min at 37°C in 3 mg/ml collagenase II (Worthington, LS004176) in HBSS supplemented with 5% FBS and 10 mM HEPES ...
-
bioRxiv - Molecular Biology 2024Quote: ... Excised hearts were cannulated to the Langendorff retrograde perfusion system through the aorta and perfused with Base solution (Tyrode’s solution with 10 mM taurine and 12 mM 2,3-butanedione monoxime) and then digested with prewarmed Digestion buffer (Base solution with 180 U/mL Collagenase Type II (Worthington, LS004177) and 25 μM CaCl2 ...
-
bioRxiv - Microbiology 2024Quote: ... The viruses were propagated in MDCK cells in the presence of L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK)-treated trypsin (Worthington Biochemical Corporation, Lakewood, NJ).
-
bioRxiv - Neuroscience 2022Quote: ... Tissue from 2-3 hypothalamiwas pooled into 1-1.5 mL of HABG containing papain (20 u/mL, Worthington Biochemical, Lakewood, NJ, US). Tissue was broken into smaller pieces by pipetting 2-3 times with a P1000 pipet followed by a fire-polished glass pipette with a wide opening ...
-
bioRxiv - Bioengineering 2022Quote: ... and then inflated via the trachea with 2-3 mL of pre-warmed (37°C) enzyme solution (1 mg/mL collagenase/dispase [Roche], 3 U/mL elastase [Worthington], 20 U/mL DNase [Worthington] in DMEM). The heart and large airways were dissected away and the remaining lung tissues and enzyme solution were collected and transferred to a shaking 37°C water bath at 75 rpm for 20 min ...
-
bioRxiv - Neuroscience 2020Quote: ... Acute bilateral fastigial nuclei were quickly excised with a sharp knife from 2 or 3 cerebellar slices and were then enzymatically digested with cysteine (2 mM)-supplemented papain (40 U/ml, Worthington, Lakewood, NJ) and chondroitinase-ABC (1 U/ml ...
-
bioRxiv - Immunology 2020Quote: To prepare single-cell suspensions lungs were finely chopped with scissors in a 48 well plate and treated with 3 mg ml-1 collagenase III (Worthington, Lakewood, NJ), 5 μg ml-1 DNAse ...
-
bioRxiv - Neuroscience 2023Quote: ... Cell pellet was collected by centrifuged at 300 g for 5 min and then resuspended in 3 mL Ovomucoid protease inhibitor with FBS (Inhibitor-BSA Vial [LK003182], Worthington, Lakewood, NJ). To remove the debris ...
Product cited:
Ovomucoid protease inhibitor and bovine serum albumin which is 0.22 micron filtered and...Cat# LK003182, 1 vi, $86.00 Ask -
bioRxiv - Cell Biology 2024Quote: ... the RPE cells were washed at least 3 times with PBS to remove the dead cells and dissociated using Papain (Worthington Biochemical Corporation) for 30mins-1h at 37 °C following the manufacturer’s guidelines ...
-
bioRxiv - Cell Biology 2021Quote: ... A single cell suspension of vascular stromal cells was isolated from minced iBAT (using scissors) digested for 90 min with collagenase IV (3 mg mL-1, Worthington, Lakewood NJ, USA) at 37 °C under gentle agitation16 ...
-
bioRxiv - Physiology 2020Quote: ... oocytes contained in 1-3 mL of ovary lobes extracted via survival surgery were isolated by enzymatic digestion with collagenase type II (Worthington Biochemical Corp., NJ, USA) under mechanical agitation ...