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Citations for Worthington Biochemical :
701 - 750 of 810 citations for 1 Benzyl 4 5 benzyloxy 6 methoxy 1 indanone 2 ylidenyl methylpiperidine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... cells were centrifuged for 5 minutes and the pellet was resuspended with DNAase/ovomucoid inhibitor according to manufacturer’s protocol (Worthington). Cells were then centrifuged and resuspended on ice in Leibovit’z L-15 medium supplemented with 0.1% of bovine serum albumin ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryonic single cell suspensions from were obtained by performing eight rounds of enzymatic digestion (5 minutes each) with Collagenase type II (0.7mg/ml Worthington) at 37°C under agitation ...
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bioRxiv - Immunology 2023Quote: ... The remaining tissue was then incubated in 5 mL of digest solution [200 U/mL Collagenase IV (Worthington, LS004189) and 400 U/mL DNase I (Worthington ...
Product cited:
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...Cat# LS004189, 5 gm, $900.00 Ask -
bioRxiv - Immunology 2024Quote: ... cut into small ∼1mm3 pieces and enzymatically digested in DMEM containing 5 mg/mL type II collagenase (Worthington, # LS004202) and 0.2 mg/mL DNase I (Roche ...
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bioRxiv - Biochemistry 2020Quote: ... Cardiomyocytes were dissociated from the ventricles by serial digestions with 0.3 mg/ml Type-2 Collagenase (Worthington) and 0.6 mg/ml pancreatin (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... the enzyme was inactivated by the addition of 2 mL of 0.71 mg/mL ovomucoid (Worthington #LS003087) and 0.06 mg/mL DNaseI (Roche #10104159001 ...
Product cited:
Mucoprotein and antitryptic factor of egg white described by Lineweaver and Murray, J. Biol....Cat# LS003087, 1 gm, $150.00 Ask -
bioRxiv - Immunology 2021Quote: ... Minced heart tissue was digested in 3 mL of digestion buffer (2 mg/mL Collagenase IV [Worthington Biochemical] ...
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bioRxiv - Cell Biology 2023Quote: ... the bone was cut into small pieces and digested with 2 mg/ml Stemzyme (Worthington, NJ, USA) for 30 min at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... Tissues were mechanically cut and digested in complete media containing Collagenase type 2 (265 U/mL; Worthington) and DNase (250 U/mL ...
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bioRxiv - Cell Biology 2022Quote: ... The isolated IVD tissue was digested in medium containing 0.2% type 2 collagenase (Worthington Biochemical, Lakewood, NK) and 0.3% pronase (Roche ...
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bioRxiv - Neuroscience 2020Quote: Brain samples were minced with scissors and then incubated at 37 °C on a roller for 30 min in digest buffer containing 1.25 mg/mL Collagenase Type 4 (Worthington, Lakewood, NJ, USA) and 100 U/mL DNAseI (Sigma ...
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bioRxiv - Developmental Biology 2023Quote: 30 hindbrains of st.18 chick embryos were harvested and disassociated into single cells using collagenase type 4 (200 Units\ml, Worthington 47B9407, USA). Cells were then centrifuged at 600 g for 10 min ...
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bioRxiv - Developmental Biology 2023Quote: ... 60 hindbrains of st.18 chick embryos were harvested and disassociated into single cells using collagenase type 4 (200 Units\ml, Worthington 47B9407, USA). Cells were then centrifuged at 600 g for 10 min ...
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bioRxiv - Bioengineering 2024Quote: ... The DRGs from 4 pups were digested for 30 min at 37°C using 13.33 U/ml papain (Worthington, cat. No. LS003126). The DRGs were then centrifuged for 1 min at 500g ...
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bioRxiv - Cancer Biology 2021Quote: ... 100-200 mg of tumor bearing lung tissue was then thoroughly minced with Noyes scissors, prior to incubation with digestion buffer (HBSS supplemented with 5% HI FBS, 125 U/mL collagenase IV (Worthington) and DNase (Roche ...
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bioRxiv - Molecular Biology 2020Quote: ... and 5 mM MgCl2) and LC-MS/MS was performed by on-bead digestion with trypsin (TPCK-treated, Worthington Biochemical).
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Harvest and Culture of Porcine Adipose-Derived Mesenchymal Stem Cells for Autologous TransplantationbioRxiv - Cell Biology 2020Quote: ... (Fat Processing Video) Phosphate-buffered saline (PBS; 100 mL) was mixed with 75 mg (±5 mg) of type A collagenase (Worthington Biochemical Corporation ...
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bioRxiv - Cancer Biology 2022Quote: ... Tumor pieces were chopped and incubated 5 min at 37°C in a HBSS-papain based lysis buffer (Worthington PAP) containing DNAse (0.01% ...
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bioRxiv - Physiology 2023Quote: ... Hearts were then perfused for 5 - 10 min with 0.125 mM CaCl2 KRH buffer supplemented with 1mg/mL of type II collagenase (Worthington Biochemical). Ventricles were cut into 0.1 mM CaCl2 solution ...
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bioRxiv - Physiology 2023Quote: ... Homogenate was digested with liver digestion buffer (5 ml HBSS w/o Ca2+/Mg2+, 200U/ml Collagenase IV (Worthington, USA) (37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... The material was then transferred to a 5 mL microcentrifuge tube containing 2.5 mL Papain supplemented with 125 uL DNase (Worthington LK003150) and incubated for 30-45 minutes in a 37°C incubator ...
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bioRxiv - Cancer Biology 2024Quote: ... the tissue was enzymatically and mechanically digested for 5-10 minutes at 37°C in HBSS-papain-based lysis buffer (Worthington) containing DNAse (0.01% ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary tumor and metastasized lung tissues were digested in 5 mL of enzyme buffer containing 0.2 mg/mL collagenase type-II (Worthington, LS004174), 0.1 mg/mL DNase I (Roche ...
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bioRxiv - Bioengineering 2022Quote: ... and then inflated via the trachea with 2-3 mL of pre-warmed (37°C) enzyme solution (1 mg/mL collagenase/dispase [Roche], 3 U/mL elastase [Worthington], 20 U/mL DNase [Worthington] in DMEM). The heart and large airways were dissected away and the remaining lung tissues and enzyme solution were collected and transferred to a shaking 37°C water bath at 75 rpm for 20 min ...
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bioRxiv - Cell Biology 2021Quote: ... Subcutaneous adipose tissue was minced and digested with collagenase I in PBS (2 mg/ml, Worthington, United States) at 37 °C for 90 minutes ...
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bioRxiv - Microbiology 2021Quote: ... 1.0 μg/mL of L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK)-treated trypsin (Worthington Biochemical, NJ, USA), 0.2% bovine serum albumin (BSA ...
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The cellular basis of protease activated receptor type 2 (PAR2) evoked mechanical and affective painbioRxiv - Neuroscience 2020Quote: ... followed by 25 minutes of incubation at 37□C in 3 mg/ml collagenase type 2 (LS004176; Worthington) and 2 mg/ml Dispase II (04942078001 ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Molecular Biology 2021Quote: ... muscles were chopped in cold PBS and digested using 800 U/ml collagenase type 2 (Worthington, cat# LS004177) in F10 medium (Gibco ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004177, 5 gm, $900.00 Ask -
bioRxiv - Immunology 2022Quote: ... lung tissue was minced and put in digestion buffer (IMDM with 183 U/ml type 2 collagenase (Worthington)) ...
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bioRxiv - Pathology 2022Quote: ... cleaned of all fat and digested for 10 min at 37°C in digestion medium (i.e. isolation medium supplemented with 1.5 mg/mL of collagenase type 2 [#LS004176, Worthington-Biochem] and 0.7 U/mL of elastase type III [#E0127 ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Physiology 2023Quote: ... and placed in a collagenase type 2 solution (500 U/mL in DMEM; Worthington Biochemical, Lakewood, NJ, USA) for 30 minutes in a tissue culture incubator (37°C ...
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bioRxiv - Immunology 2023Quote: ... Lungs were transferred into Miltenyi Gentle MACS tubes containing 2 mL of 2mg/mL collagenase II (Worthington #LS004177) and mechanically ground up by a Gentle MACS machine ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004177, 5 gm, $900.00 Ask -
bioRxiv - Neuroscience 2023Quote: ... Crude collagenase (Col) type 2 (>125 units per mg; Cat: LS004214) preparations were acquired from Worthington (Lakewood, NJ) and complete Freund’s adjuvant (CFA ...
Product cited:
Collagenase, Type 1 (Code: CLS-1), which is filtered through a 0.22 micron membrane and...Cat# LS004214, 50 mg, $55.00 Ask -
bioRxiv - Cancer Biology 2023Quote: ... The tissue was transferred to a pre-warmed 0.125U/mL collagenase solution (Collagenase Type 2, Worthington Biochemical, LS004176) prepared in DMEM (10% FBS ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Physiology 2024Quote: ... cut into small fragments and dissociated in 2 mL HBSS containing 3 mg/mL collagenase type II (Worthington), 7.5mg/mL dispase II and 0.25 mg/mL DNase I for 30 minutes at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... digested for 30 min at 37°C in 2 mg/mL collagenase II (Worthington Biochemical, Lakewood, NJ, USA) in DMEM containing 100 U/mL penicillin and 100 µg/mL streptomycin (GibcoTM ...
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bioRxiv - Immunology 2023Quote: ... and then digested for 30 minutes under gentle agitation at 37°C with 7 mL of 4 mg/mL of type III collagenase (Worthington Biochemical, Lakewood, NJ) and 0.05 mg/mL of DNase I (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... SNc regions from the slices were dissected out with a scalpel blade and digested with oxygenated high glucose HEPES-buffered saline containing papain (5–8 U/ml, Worthington, USA) for 20–30 min at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... the dissociation buffer was warmed to 34□ and 5 mL was added to a vial of lyophilized papain (Worthington Biochemical Corporation). The solution was diluted 1:2 with additional dissociation buffer prior to sample dissociation.
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bioRxiv - Molecular Biology 2022Quote: ... Primary osteoblasts were isolated from the calvaria of 5-day-old mouse pups using collagenase type II (50 mg/ml, Worthington, LS004176)/dispase II (100 mg/ml ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Physiology 2020Quote: ... This was followed by a wash step and centrifugation at 500 x g for 5 min before removing the dissociation buffer and adding stock collagenase II (4.3 mg/ml) (Worthington, Lakewood, USA) and stock dispase (6.0 mg/ml ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Primary calvarial osteoblasts were isolated from calvaria of 5-day-old neonates using collagenase type II (50 mg/ml, Worthington, LS004176)/dispase II (100 mg/ml ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004176, 1 gm, $215.00 Ask -
bioRxiv - Neuroscience 2024Quote: Cortical assembloids were washed with 1X PBS and dissociated at 37°C 5% CO2 on an orbital shaker with Papain (Worthington #LK003150) for 30 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... 3 sutures each from litter 2) were cut into small fragments in HBBS and digested using Collagenase IV (Worthington, USA ...
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bioRxiv - Genetics 2020Quote: ... the cells were resuspended in EBSS containing 50μg/mL of DNase I and 2 mg/mL ovomucoid (Worthington, LK003150). 0.5 volume of EBSS ...
Product cited:
Set of five single use vials of papain and five single use vials of DNase, 100 ml of Earle's...Cat# LK003150, 1 bx, $295.00 Ask -
bioRxiv - Cancer Biology 2020Quote: Lungs were extracted and placed into dissociation buffer that was composed of 1mg/ml collagenase type 2 (Worthington, #LS004177), 0.25U/ml neutral protease (Worthington ...
Product cited:
Prepared to contain higher clostripain activity. Suggested for bone, heart, liver, thyroid and...Cat# LS004177, 5 gm, $900.00 Ask -
bioRxiv - Immunology 2020Quote: ... The lysate was then subjected for digestion using L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK) treated trypsin (Worthington Biochemical Corporation ...
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bioRxiv - Immunology 2020Quote: ... The lysate was then subjected for digestion using L-(tosylamido-2-phenyl) ethyl chloromethyl ketone (TPCK) treated trypsin (Worthington Biochemical Corporation ...
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bioRxiv - Cancer Biology 2023Quote: ... 2×106 HCT116 cells were used per each immunoprecipitation (IP) reaction and 15 units of micrococcal nuclease (MNase, Worthington) was used to digest the chromatin for the ChIP reaction ...
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bioRxiv - Bioengineering 2024Quote: ... the tissue was first incubated in an enzymatic solution containing 2 mg/mL collagenase II (Worthington Biochemical, Lakewood, NJ) in HyClone Low Glucose Dulbecco’s modified Eagle’s medium (Cytiva ...