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Citations for Qiagen :
151 - 200 of 1778 citations for Two Hybrid cDNA Library since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... and reverse transcribed into cDNA using RT2 first strand cDNA synthesis kit (Qiagen, Hilden, Germany), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Total RNA to cDNA conversion was done using RT2 first strand cDNA synthesis kit (Qiagen). Prepared cDNA was subjected to a customised qRT – PCR array (SABiosciences ...
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bioRxiv - Neuroscience 2022Quote: ... and aged (n=10) RVLM-containing tissue punches was reverse transcribed to cDNA using an RT2 preamp cDNA synthesis kit (Qiagen, Inc., First strand cDNA synthesis) following manufacturer’s guidelines ...
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bioRxiv - Developmental Biology 2022Quote: ... Libraries were prepared in-house using the Ultra-low Input Library kit (Qiagen; #180495). Libraries were sequenced with single-end 50 bp reads on the illumina HiSeq 4000 sequencing platform (n=5 samples per experimental group).
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bioRxiv - Cancer Biology 2022Quote: Small-RNA Library preparations were executed with QIAseq miRNA Library Preparation Kit (1103679, Qiagen) according to the manufacturer’s instructions using multiplexing adapters ...
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bioRxiv - Microbiology 2019Quote: ... Genomic DNA libraries were prepared using a Nextera™ NGS Library Preparation Kit (QIAGEN). Libraries were sequenced at Glasgow Polyomics (www.polyomics.gla.ac.uk/index.html) ...
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bioRxiv - Neuroscience 2020Quote: Sequencing libraries were prepared using the QIAseq FX Single Cell RNA Library Kit (Qiagen) according to manufacturer instructions ...
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bioRxiv - Immunology 2021Quote: ... The small RNA library was prepared using the QIAseq miRNA library kit (331502, Qiagen), specifically optimized for very low input small RNA ...
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bioRxiv - Genomics 2022Quote: ... DNA sequencing libraries were produced using the GeneRead DNA Library I Core Kit (Qiagen) and Netflex Dual-index DNA Barcodes (Perkin Elmer ...
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bioRxiv - Microbiology 2022Quote: ... and library quantification was done with the QIAseq Library Quant Assay Kit (Qiagen, Germany). Libraries were sequenced using an Ion 530 chip and chemistry for 400 base pair reads on an Ion Torrent S5XL instrument (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... The libraries were then prepared using the Qiaseq Ultra Low Input Library Kit (Qiagen), according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: DNA libraries were prepared using the QiaSeq UltraLow Input library kit (Qiagen, Germantown, Australia) using custom dual-indexed adapters ...
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bioRxiv - Synthetic Biology 2021Quote: ... Libraries were gel purified (Qiagen) between 250 and 550bp in size ...
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bioRxiv - Developmental Biology 2020Quote: ... Libraries were gel purified (Qiagen) between 250 and 550bp in size ...
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bioRxiv - Cancer Biology 2023Quote: ... Barcoded libraries (One-step, Qiagen) were then run on MiSeq or NextSeq Illumina sequencers ...
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bioRxiv - Microbiology 2020Quote: ... Two methods of extraction were used: the DNeasy Blood and Tissue Kit (Qiagen) and the DNeasy PowerSoil Kit (Qiagen) ...
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bioRxiv - Molecular Biology 2022Quote: Two independent siRNAs targeting YBX1 (Hs_YBX1-1 and Hs_YBX1-6 FlexiTube siRNA, Qiagen) and a non-targeting negative control siRNA (AllStars ...
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bioRxiv - Physiology 2022Quote: ... Two rounds of tissue homogenization were performed on the TissueLyserII machine (Qiagen, #85300) at 25 Hz ...
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bioRxiv - Biochemistry 2021Quote: ... 2.5 × 108 cells were mixed with two volumes of RNAprotect Bacteria Reagent (Qiagen). Samples were lysed in Tris-EDTA (pH 8.0 ...
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bioRxiv - Neuroscience 2021Quote: ... and two rounds of washes using the RNAeasy Mini Kit (Qiagen, Cat# 74106). Total RNA concentration was determined with the Nanodrop 1000 (v3.8.1 ...
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bioRxiv - Plant Biology 2023Quote: ... and ground for two minute at 20 Hz in a TissueLyser II (QIAGEN). Then ...
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bioRxiv - Biochemistry 2023Quote: ... the supernatant was filtered and loaded on two 0.5 ml Ni-NTA (Qiagen) columns (one column/L growth ...
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bioRxiv - Cancer Biology 2024Quote: ... Two ml of a 50% slurry of washed Ni-NTA beads (Qiagen # 30210) was added to the supernatant of the 2-liter culture ...
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bioRxiv - Cancer Biology 2019Quote: ... 1 μg of RNA was reverse-transcribed into cDNA using the RT Omniscript cDNA kit (Qiagen), and 20 ng of cDNA template was amplified on a LightCycler-480 (Roche ...
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bioRxiv - Immunology 2020Quote: ... Isolated RNA was converted to complementary DNA (cDNA) using RT2 first strand cDNA synthesis kit (Qiagen). The cDNA thus obtained was subjected to quantitative real-time PCR analysis with RT2 SYBR® Green qPCR Mastermix (Qiagen ...
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bioRxiv - Immunology 2023Quote: ... according to manufacturer’s instructions and converted to cDNAs employing RT2 HT First Strand cDNA Kit (Qiagen). qPCR was performed with FastStart Universal SYBR Green Master reagents (Roche ...
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bioRxiv - Microbiology 2023Quote: ... RNA samples were used as templates to synthesize cDNA using a cDNA synthesis kit (Qiagen, Germany). The resultant cDNA was then used for qRT-PCR using the LightCycler 96 Real-Time PCR system (Roche Diagnostics ...
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bioRxiv - Physiology 2021Quote: ... cDNA was purified with QiaQuick (Qiagen) and expression quantified on an Applied Biosystems thermocycler ...
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bioRxiv - Microbiology 2023Quote: ... and cDNA produced (QuantiTech RT, Qiagen). Primers for Jamaican fruit bat genes (Supplemental Table 2 ...
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bioRxiv - Developmental Biology 2021Quote: A miRNA sequencing library was prepared using the QIASeq miRNA Library Kit (QIAGEN, Hilden, Germany) from total RNA ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The next-generation sequencing (NGS) libraries were prepared using QIAseq FX DNA Library kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Each phage DNA library was constructed using the QIA seq FX DNA library kit (Qiagen), and sequencing was performed using the Illumina MiSeq platform ...
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bioRxiv - Microbiology 2023Quote: ... DNA libraries were prepared from the chromatin using a Qiagen FX 96 Library Kit (QIAGEN) for sequencing using the Illumina NextSeq 500 platform ...
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bioRxiv - Developmental Biology 2023Quote: ... sRNA-seq libraries were made using the QIAseq® miRNA library preparation kit (Qiagen #331502), according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2019Quote: DNA from parental and all second-generation hybrid offspring (KonPar: N = 263; PruKoh: N = 193; CerEuk: N = 230) was isolated using the DNeasy Blood & Tissue Kit (Qiagen, Valencia, CA, USA) and sequenced following the Genotype-By-Sequencing (GBS ...
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bioRxiv - Genetics 2023Quote: ... RNAs were reverse transcribed to cDNAs using the Quantabio qScript cDNA synthesis kit (QIAGEN, Beverley, MA, USA). The cDNAs were then used for qPCR using qPCRBIO SyGreen Blue Mix Lo-ROX (PCR Biosystems ...
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Building a robust chromatin immunoprecipitation (ChIP) method with substantially improved efficiencybioRxiv - Plant Biology 2020Quote: ... DNA was purified from these two portions using a DNA purification spin column (Qiagen), and eluted with 60 µl of TE buffer ...
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bioRxiv - Microbiology 2020Quote: ... which included two separate DNA digestions (on-column then off-column) with DNAse (Qiagen), all according to manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2020Quote: ... were purified by two rounds of nickel affinity chromatography using Ni-NTA agarose (QIAGEN), with overnight cleavage by N-terminally His-tagged TEV protease (expressed in our laboratory ...
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bioRxiv - Systems Biology 2022Quote: ... confirmed by two PCRs (Supp. Fig. 1B and 1B′) by HotStarTaq Master Mix (Qiagen) using the following primers ...
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bioRxiv - Genomics 2019Quote: ... RNA from two brains were extracted using the RNeasy Mini Kit (Qiagen, Valencia, CA) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... Two µg of each DNA sample was bisulfite modified using EpiTect Bisulfite kit (Qiagen). The Pparγ1sv promoter region was amplified with EpiTaq HS polymerase (Takara ...
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bioRxiv - Microbiology 2021Quote: ... The stage two PCR products were purified with the MinElute PCR purification kit (Qiagen) following manufacturer protocols ...
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bioRxiv - Immunology 2020Quote: Two milliliters of whole blood cryopreserved in PAXgene Blood RNA Tubes (cat.nr. 762165, Qiagen) were subjected to RNA isolation with QIAcube using PAXgene Blood RNA Kit (cat.nr ...
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bioRxiv - Biochemistry 2021Quote: ... and the supernatant was applied to two 5 ml Ni-NTA Superflow cartridges (Qiagen). The columns were washed with 20 mM HEPES pH 8.0 ...
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bioRxiv - Plant Biology 2022Quote: Samples were ground with two zirconia beads using a TissueLyser II (QIAGEN, MD, USA) with pre-chilled adapters at −80 °C ...
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bioRxiv - Cell Biology 2022Quote: ... and homogenized for two cycles with a Bead Beater TissueLyser II (Qiagen, Germantown, MD) at 25 Hz for 3 min each ...
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bioRxiv - Microbiology 2022Quote: Two siRNA duplex target sequences (siHSF11 and siHSF12) and their scrambled control (scrRNA) (QIAGEN) were used for HSF1-silencing (Supplementary Table S2) ...
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bioRxiv - Neuroscience 2022Quote: ... Two to 10 ng of genomic DNA was whole genome amplified (Repli-G, Qiagen) and digested with AflII and SspI ...
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bioRxiv - Plant Biology 2023Quote: ... The sample was subsequently homogenized for two cycles at 2000 rpm (PowerLyzer 24, Qiagen). The steel beads were removed ...