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Citations for Qiagen :
51 - 100 of 1778 citations for Two Hybrid cDNA Library since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... RNA was normalised to the lowest sample concentration and cDNA libraries prepared using the QuantiTect® Reverse Transcription Kit (QIAGEN, UK) as per manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... and the hybrids was isolated from the muscle tissue using DNeasy Blood & Tissue Kits (Qiagen). The quality of DNA was checked by a NanoDrop® ND-1000 spectrophotometer with 260/280 and 260/230 ratios ...
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bioRxiv - Molecular Biology 2024Quote: RNA isolation from leaf tissues of five-week-old non-transformed wild type and overexpressing transgenic lines (two replicates of wild type and two transgenic lines) were isolated as per manufacturer’s instruction (Qiagen, Germany). High quality RNA samples with 200 ng/μL concentration were outsourced for RNA-seq ...
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bioRxiv - Molecular Biology 2021Quote: ... and loaded onto two nucleotide removal columns (Qiagen). Qiagen buffer PNI can be replaced with a mixture of 1 volume Qiagen buffer PB to 0.75 volumes isopropanol ...
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bioRxiv - Microbiology 2022Quote: ... Qiagen DNeasy Blood and Tissue Kits were used to extract DNA while The Qiagen RNEasy kit was used to complete RNA extraction and cDNA library preparation (Qiagen, Hilden, Germany). Shotgun DNA and RNA sequencing was completed at the University of Michigan Sequencing Core using the Illumina® HiSeq™ platform (2000 PE 100 ...
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bioRxiv - Molecular Biology 2021Quote: Approximately 10 to 20 mg of frozen muscle was homogenised two times for two minutes at a speed of 30 Hz with a TyssueLyser instrument (Qiagen, Canada) in an ice-cold lysis buffer (1:20 w/v ...
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bioRxiv - Cancer Biology 2020Quote: ... The total cell RNA of two control samples and two treated samples were extracted with RNeasy Kit (Qiagen, Cat No. 74106), and then processed and sequenced by BGI Genomics (BGI Genomics ...
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bioRxiv - Genomics 2022Quote: ... we extracted RNA from freshly dissected tissue of two male and two female singing mice (liver and brain) using a Qiagen RNeasy Mini kit (Qiagen 74104). We then homogenized the tissue using a rotor-stator homogenizer with disposable tips and did on-column DNAse digestion following manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... and viral RNA was isolated from infected cells using a hybrid TRIzol and RNeasy extraction (Qiagen) protocol ...
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bioRxiv - Bioengineering 2023Quote: ... F1 hybrid offspring were collected in bulk as late-stage embryos and were DNA extracted (Qiagen, DNeasy Blood & Tissue Kits ...
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bioRxiv - Neuroscience 2023Quote: ... Reverse transcription with 50 ng of RNA into a cDNA library was performed using the Omniscript RT kit (#205111, QIAGEN, Inc.; Germantown, MD) and random nonamers (Integrated DNA Technologies ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and two times 500 μL or RPE buffer (Qiagen) with centrifugation for 15 sec at 13,000 x g ...
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bioRxiv - Microbiology 2019Quote: ... with two sets of 96-well adapter plates (QIAGEN) containing 2 ml microcentrifuge tubes (QIAGEN ...
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bioRxiv - Cancer Biology 2021Quote: ... we used the same set of two siRNAs (Qiagen) against HNF1B compared with negative and positive control siRNA (Qiagen) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... with two sterile 3 mm beads (Qiagen, Hilden, Germany) and a bead device at 20 Hz for 3 min ...
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bioRxiv - Microbiology 2019Quote: ... Two separate rounds of DNase treatment were performed (Qiagen RNase-free DNase ...
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bioRxiv - Genetics 2019Quote: ... Repaired DNA was purified on two MinElute columns (Qiagen) and eluted in 16 μl (32 μl final for each library) ...
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bioRxiv - Microbiology 2019Quote: ... Cultures were treated with two volumes of RNAprotect (Qiagen) incubated for 10 min at room temperature ...
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bioRxiv - Genetics 2019Quote: ... Repaired DNA was purified on two MinElute columns (Qiagen) and eluted in 16 μl (32 μl final for each library) ...
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bioRxiv - Microbiology 2023Quote: ... immediately mixed with two volumes of RNAProtect Bacteria (Qiagen) and pelleted according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was prepared by miScript RT II cDNA kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: Two antisense LNA GapmeRs specifically targeting two different regions of LINC00607 (NR_037195.1) were designed and purchased from QIAGEN (see table below for sequences). LNAs were separately transfected into HUVECs with Lipofectamine RNAiMAX following the protocol provided by the manufacturer ...
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bioRxiv - Genetics 2022Quote: ... Quantified libraries (GeneRead DNA Library L Core Kit; QIAGEN) were sequenced using an Ion Torrent S5 XL instrument with Ion 530 chips and respective reagents (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... total RNA was extracted from NPE cells and reverse-transcribed to produce a cDNA library according to the manufacturer’s protocols (Qiagen; #74104 and Thermo Scientific; #K1621). The Ilk gene was amplified from the cDNA library using primers introducing NotI and BamHI restriction sites into the 5’- and 3’-regions of the gene ...
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bioRxiv - Genomics 2019Quote: ... Two volumes of RNAprotect Bacteria Reagent (Qiagen, Valencia, CA, USA) were added to all samples and incubated at room temperature for 5min ...
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bioRxiv - Microbiology 2023Quote: ... with two 5-minute cycles on a TissueLyser II (Qiagen) separated by a 5-minute incubation on ice ...
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bioRxiv - Immunology 2024Quote: ... Two PCRs were performed with the PyroMark PCR kit (Qiagen) in order to report the average methylation of 8 representative CpG sites in the regulatory T cell–specific demethylated region (TSDR ...
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bioRxiv - Cancer Biology 2019Quote: ... Libraries were quantified using the QIAseq Library Quant Assay (QIAGEN) and the fragment size was checked using the Bioanalyzer High Sensitivity DNA Kit (Agilent Technologies) ...
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bioRxiv - Microbiology 2021Quote: ... library preparation using Qiagen FastSelect and Library Prep kits (Qiagen) and Ribo-Zero Plus kit (Illumina) ...
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bioRxiv - Plant Biology 2022Quote: ... sRNA libraries were constructed with QIAgen miRNA library (QIAgen #331502) and were sequenced on an Illumina HiSeqV4 SR50 ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were prepared using “QIASeq miRNA Library kit” (Qiagen, Germany) and sequenced as 2x75bp on a NextSeq 550 (Illumina ...
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bioRxiv - Developmental Biology 2023Quote: ... libraries were prepared with QIAseq FX DNA Library Kit (QIAGEN) and sequenced on the Illumina MiSeq platform (Illumina ...
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bioRxiv - Bioengineering 2021Quote: ... cDNA was synthesized using the Quantabio Qscript miRNA cDNA synthesis kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was purified from cells using the Trizol/RNeasy hybrid protocol (Trizol, Life Technologies; RNeasy Mini Kit, Qiagen). Polyadenylated [poly(A)] RNA was then purified with the NEBNext Poly(A ...
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bioRxiv - Evolutionary Biology 2020Quote: ... n=4 females [hybrid cross] and n=5 females [conspecific cross]) using the AllPrep RNA/DNA Mini Kit (Qiagen), and was stored at −80°C until sequencing ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were prepared using QIAseq Ultralow Input Library Kit (Qiagen, #180492). Final libraries were directly PCR amplified from Streptavidin beads ...
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bioRxiv - Cancer Biology 2021Quote: ... Library prep was prepared using QIAseq Ultralow Input Library Kit (Qiagen). KAPA qPCR assay was performed to estimate concentration and cycle number for final PCR ...
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bioRxiv - Microbiology 2022Quote: ... Libraries were quantified with the QIAseq Library Quant Assay Kit (Qiagen) and sequenced on an Ion Torrent S5XL instrument using Ion 530 chips and chemistry for 400 bp reads ...
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bioRxiv - Immunology 2021Quote: ... Libraries were generated by using the Qiaseq RNA library kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: Small RNA libraries were generated using QIAseq miRNA Library Kit (QIAGEN) according to the manufacturer instructions ...
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bioRxiv - Plant Biology 2022Quote: sRNA libraries were generated with QIAgen miRNA library kit (QIAgen #331502) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were prepared using the QIAseq microRNA Library Kit (Qiagen #331502) and sequenced using Illumina NovaSeq SP run with 100 base single-end reads ...
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bioRxiv - Cell Biology 2023Quote: ... MiRNA libraries were prepared using the QIASeq miRNA Library Kit (Qiagen). Each resulting indexed library was quantified and its quality assessed by Qubit and Agilent Bioanalyzer ...
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bioRxiv - Developmental Biology 2019Quote: ... The two supernatants were combined and purified with MinElute kit (Qiagen) in 22µl of EB buffer ...
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bioRxiv - Cancer Biology 2019Quote: ... Two pathway-directed RT2 Profiler PCR Arrays were selected from Qiagen: Mouse Extracellular Matrix & Adhesion Molecules (PAMM-013ZE-4 ...
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bioRxiv - Genomics 2020Quote: ... Supernatant was loaded on two 1-ml Ni-NTA (Qiagen 30210) columns ...
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bioRxiv - Immunology 2022Quote: ... The tissue homogenization was performed using two Tungsten Carbide Beads (Qiagen) (3 mm ...
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bioRxiv - Microbiology 2022Quote: ... Cell cultures were then combined with two volumes of RNAprotect (Qiagen) in an anaerobic chamber ...
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bioRxiv - Microbiology 2023Quote: ... two swabs pre-moistened in nuclease-free water (Qiagen, Hilden, Germany) were inserted 2 cm into one nostril and rotated against the anterior nasal mucosa for up to 10 seconds ...
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bioRxiv - Microbiology 2023Quote: ... Cultures were stopped by addition of two volumes of RNAprotect (Qiagen) then centrifuged at 5,000 g for 5 min ...