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Citations for Qiagen :
1 - 50 of 1778 citations for Two Hybrid cDNA Library since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... (5) hybrid libraries were then eluted in 40μL of elution buffer (Qiagen, 19086).
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bioRxiv - Genomics 2020Quote: Two sets of libraries were constructed using the QIAseq FX single cell RNA library kit (Qiagen, Germany), with one set coupled with the QIAseq FastSelect rRNA HMR kit only (Protocol 2) ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA libraries were constructed using QIAseq miRNA Library Kit (Qiagen, Cat:331505 & 331595) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and cDNA libraries were generated (Qiagen Total Stranded RNA). cDNA libraries were sequenced using NextSeq 550 using a 75bp x 8bp x 8bp setup ...
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bioRxiv - Neuroscience 2019Quote: ... cDNA libraries were created using QuantiTect Reverse Transcription Kit (Qiagen). Quantitative PCR was performed using Sybr Green Master Mix (Applied Biosystems Systems ...
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bioRxiv - Developmental Biology 2021Quote: ... hybrid embryos and sorted cells were prepared using QIAseq miRNA Library Prep with single index or UDI kit (Qiagen, 331502) and sequenced on HiSeq 65bp single-end sequencing or NovaSeq 50bp paired-end sequencing platform ...
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bioRxiv - Bioengineering 2023Quote: ... The bands representing the protein hybrid libraries were excised and DNA was purified using the QIAquick Gel Extraction Kit (Qiagen). The DNA concentration was then measured with the Quant-iT dsDNA (HS ...
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bioRxiv - Plant Biology 2020Quote: ... The cDNA libraries were purified using MinElute Gel Extraction Kits (Qiagen) and quantified with KAPA Library Quantification kits (Roche ...
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bioRxiv - Microbiology 2023Quote: ... cDNA libraries were purified using the QIAquick PCR Purification Kit (QIAGEN) and sequenced using a HiSeq 1500 machine (Illumina ...
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bioRxiv - Physiology 2020Quote: ... DNA libraries were subjected to two rounds of bisulfite modification with the EpiTect Bisulfite Kit (Qiagen) following by 12-cycles of PCR amplification ...
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bioRxiv - Physiology 2022Quote: ... Two uL of total RNA was used to synthetized cDNA using the miRCURY LNA RT kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2022Quote: ... The cDNA library was purified using the MinElute PCR purification kit (Qiagen) and amplified using Q5 Host-Start High-Fidelity 2x Master Mix (New England Biolabs) ...
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bioRxiv - Molecular Biology 2023Quote: We performed qRT-PCR to determine the optimal number of PCR cycles for cDNA amplification to generate a sufficient amount of cDNA for library construction (cycler: Rotor-Gene-Q, Qiagen, Germany ...
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bioRxiv - Cell Biology 2021Quote: ... A total of 5 μl of purified RNA tagged with adapters containing a Unique Molecular Index (UMIs) was converted into cDNA NGS libraries using the QIAseqmiRNA Library Kit (QIAGEN). cDNA was amplified in 22 cycles of PCR and purified ...
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bioRxiv - Molecular Biology 2024Quote: A total of 5 ng of EV-RNA/sample and 100 ng of cellular RNA/sample were used as a template for the preparation of cDNA libraries using the QIAseq miRNA Library Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA library was obtained after 35 cycles of amplification (PCR core kit, Qiagen). Primer pairs (Forward ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA synthesis for two-step RT-PCR was performed using the QuantiTect Reverse Transcription Kit (cat 205313) from QIAGEN. For each sample ...
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bioRxiv - Microbiology 2023Quote: ... cDNA library was prepared by using the QuantiTect reverse transcription kit (Qiagen, Valencia, CA). The cDNA library was then sent to the Genomic Services Laboratory at HudsonAlpha Genome Sequencing Center (Huntsville ...
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bioRxiv - Plant Biology 2023Quote: ... Adaptor-ligated libraries were subjected to two successive treatments of sodium bisulfite conversion using the EpiTect Bisulfite kit (Qiagen) as outlined in the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Adaptor-ligated libraries were subjected to two successive treatments of sodium bisulfite conversion using the EpiTect Bisulfite kit (Qiagen) as outlined in the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... ~250 ng of pooled cDNA of each sample was used as input for library preparation using QIAseq FX DNA library kit (QIAgen, Cat.# 180475) or Nextera DNA Flex library Prep kit (illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... A total of 1 ng of RNA from microdissected human pancreatic islets and from EndoC-βH1 was used to generate cDNA libraries using QiaSeq miRNA library kit (Qiagen, Hilden, Germany) following manufacturer’s instructions (see ESM Methods).
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bioRxiv - Microbiology 2021Quote: ... we used both one-step RT-PCR and two-step (cDNA synthesis followed by separate PCR amplification) reaction designs with commercial One-Step Ahead RT-PCR Kit (Qiagen) or IEH in-house RT ...
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bioRxiv - Developmental Biology 2023Quote: ... circularized DNA was purified with AMPure XP beads and 4C-libraries were finally generated by two rounds of PCR and purified by QIAGEN column ...
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bioRxiv - Genomics 2020Quote: ... Pre-capture libraries with less than 100 ng of double-stranded cDNA (n=5; PT0017_Qiagen_20ng_XTHS ...
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bioRxiv - Cell Biology 2022Quote: cDNA and library preparation and sequencing: RNA was isolated using RNeasy Mini Kits (QIAGEN, 74104) from HepG2 cells co-cultured for 20 hours with or without neutrophils ...
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bioRxiv - Genetics 2019Quote: ... hybrid DNA was extracted using a DNeasy® kit (Qiagen). DNA concentrations were measured using a QubitTM 2.0 fluorometer (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Two kit controls (Qiagen glass filter and PALL ...
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bioRxiv - Cell Biology 2023Quote: ... Two on-column (Qiagen) and one in-solution (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... Two on-column (Qiagen) and one in-solution DNase (NEB ...
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bioRxiv - Neuroscience 2021Quote: To prepare cDNA libraries collected frozen tissue was processed using a QIAGEN RNeasy kit (QIAGEN, #74034) to extract RNA and prepare cDNA libraries using SMARTseq v4 kit (Clontech ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA library was prepared from 1,500 ng of RNA using QIAseq FastSelect -rRNA HMR Kit (Qiagen) and NEBNext® Ultra™ II RNA Library Prep Kit for Illumina (New England Biolabs) ...
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bioRxiv - Immunology 2022Quote: ... two rounds of nested PCR reactions were carried out using the cDNAs as template and Hot Start DNA Polymerases (Qiagen, Thermo Fisher) and specific primer sets described previously 86 ...
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bioRxiv - Immunology 2020Quote: ... and light (Vκ and Vλ) genes were amplified separately from the pooled cDNA samples by two rounds of PCR using HotStarTaq (Qiagen, Cat# 203443) and human antibody sequences primers derived from previous report15 ...
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bioRxiv - Microbiology 2019Quote: ... Two volumes of RNAprotect (Qiagen) was added to each sample and the samples were incubated for 10 minutes at 25 °C ...
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bioRxiv - Microbiology 2020Quote: ... Two volumes of RNAprotect (Qiagen) was added to the cultures ...
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bioRxiv - Molecular Biology 2022Quote: ... Two volumes RNA protect (Qiagen) was added to the culture prior to RNA extraction using Monarch total RNA miniprep kit (NEB) ...
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bioRxiv - Cancer Biology 2019Quote: Total RNA of 500 ng was used for preparing cDNA libraries using QuantiTect Reverse Transcription Kit (Qiagen, USA). Gene expression was estimated by QuantStudio 12K Flex Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Neuroscience 2020Quote: ... A cDNA library was created form each mRNA sample using the Omniscript RT kit (205113, Qiagen, Valencia CA) with random nonamers (Integrated DNA Technologies ...
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bioRxiv - Cancer Biology 2021Quote: Individual set of two siRNAs (Qiagen) against HNF1B or ERG were tested in knockdown efficiency and compared with the siRNA negative-control (Qiagen ...
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bioRxiv - Cell Biology 2020Quote: ... RNA (500 ng) was reverse transcribed to produce cDNA libraries using the QuantiTect Reverse Transcriptase Kit from Qiagen (205311). cDNA was quantified by Qubit analysis with the 1× dsDNA HS Assay Kit (Thermo Fisher Scientific ...
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cDNA library screening to identify interacting proteins of Golgi-localized type II membrane proteinsbioRxiv - Molecular Biology 2020Quote: ... The cDNA libraries were amplified in DH10B E.coli cells and purified using QIAGEN Plasmid Mega Purification kit (Qiagen, Germany). The average size of the cDNA inserts into pPR3-N was evaluated by purifying 20 random colonies each and digesting with SfiI ...
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bioRxiv - Cancer Biology 2021Quote: ... 500 ng of total RNA was used for establishing the cDNA library with the miScript II RT Kit (Qiagen). qRT-PCR was performed with the SYBR Green I Master kit (Roche Applied Science ...
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bioRxiv - Microbiology 2020Quote: ... Two mouse and two human stool samples were homogenized using a PowerLyzer 24 Homogenizer (110/220V; Qiagen). DNA was extracted using four different bead beating times ...
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bioRxiv - Molecular Biology 2019Quote: Total RNA was extracted from hybrid ESCs and NPCs using the miRNEasy Kit (Qiagen) and DNaseI treatment (Qiagen) ...
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bioRxiv - Microbiology 2022Quote: ... LF/LF = 5 mice from two cages over two experiments) using the DNeasy PowerSoil Kit (Qiagen, Germantown, MD). Modifications to the standard protocol included a 10-minute incubation at 65°C immediately following the addition of the lysis buffer and the use of a bead mill homogenizer at 4.5 m/s for 1 min ...
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bioRxiv - Neuroscience 2019Quote: ... cDNA libraries of miRNAs were created from 20ng of total RNA with the mirCURY LNA RT Kit (Qiagen, Germantown, MD). PCR reactions were performed using the miRCURY LNA SYBR Green PCR Kit and the following locked nucleic acid (LNA ...
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bioRxiv - Microbiology 2020Quote: ... Two volumes of RNAprotect Reagent (Qiagen, Germany) are added into the cultures according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: Two biological replicates of DS1 and two biological replicates DS2U hiAs were harvested in RLTplus Lysis buffer (Qiagen, 1053393). Total RNA was isolated using the RNeasy micro/mini plus kit (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... for two cycles of two minutes each at an oscillation frequency of 30/second using a TissueLyser II (Qiagen). RNA was extracted using the miRNeasy mini kit (Qiagen) ...