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Citations for Qiagen :
401 - 450 of 1718 citations for Dengue Virus serotype 2 lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Total Nucleic Acid (TNA) was extracted from using automated nucleic acid extraction on the QIAsymphony and the DSP Virus/Pathogen Mini Kit (Qiagen). Autopsy tissues were collected from lung ...
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bioRxiv - Microbiology 2022Quote: ... Viral RNA was extracted from supernatants of hamster nasal wash samples using the QIAsymphony DSP Virus/Pathogen Mini Kit on the QIAsymphony instrument (Qiagen). Real time RT-qPCR was then performed using the AgPath RT-PCR (Life Technologies ...
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bioRxiv - Microbiology 2022Quote: Viral RNA extraction was performed on an EZ1 Advanced XL device using the EZ1 virus mini kit V2.0 according to the manufacturer’s recommendations (Qiagen, Courtaboeuf, France). The qPCR was run on a Lightcycler® 480 thermocycler (Roche diagnostics ...
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bioRxiv - Microbiology 2022Quote: ... Viral RNA was extracted from cell culture supernatants or hamster nasal washes using the QIAsymphony DSP Virus/Pathogen Mini Kit on the QIAsymphony instrument (Qiagen). RT-qPCR was then performed using the AgPath RT-PCR (Life Technologies ...
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bioRxiv - Molecular Biology 2020Quote: Total viral RNA was extracted from deactivated samples using automated nucleic acid extraction on the QIAsymphony and the DSP Virus/Pathogen Mini Kit (QIAGEN). One step reverse transcription to cDNA and real-time PCR (RT-PCR ...
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bioRxiv - Microbiology 2020Quote: ... MB897-1) were used to generate infectious virus by transfecting 293T cells using the Effectene transfection reagent (QIAGEN, Valencia, CA). Supernatant containing virus was collected 2 days post transfection ...
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bioRxiv - Microbiology 2020Quote: ... viral RNA was extracted from either DF-1 cultures infected with the WT virus or DF-1P cells using the Qiamp viral RNA mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... EAV genome copy number was determined by one step RT-qPCR using the QuantiTect™ Virus + ROX Vial Kit (Qiagen). Each RNA sample was tested in triplicate ...
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bioRxiv - Physiology 2021Quote: ... We infected Sf9 cells with the recombinant virus to express and purify the His-tagged proteins using a nickel-nitrilotriacetic acid (Ni-NTA, Qiagen) column ...
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bioRxiv - Evolutionary Biology 2020Quote: ... RNA from the original material used for next-generation sequencing of the serum and passage 3 cultured MRI103 virus was used as the template for amplification using the One-Step Ahead RT-PCR kit (Qiagen).
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bioRxiv - Microbiology 2020Quote: ... virus pellets were resuspended by vortexing and community DNA was extracted using the QIAamp Viral RNA Mini Kit (Qiagen, Germany) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... Total Nucleic Acid (TNA) was extracted using automated nucleic acid extraction on the QIAsymphony and the DSP Virus/Pathogen Mini Kit (Qiagen). RNA isolation and library preparation is fully described in Butler ...
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bioRxiv - Immunology 2022Quote: ... we isolated viral RNA directly from the Fluenz Tetra vaccine (AstraZeneca; season 2017/2018) virus using the Qiagen Viral RNA Isolation Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... The cells were cultured in growth media containing virus for 60 hours and total RNA was then extracted with the RNeasy kit (Qiagen). From the total RNA ...
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bioRxiv - Microbiology 2023Quote: Total genomic DNA from the bacteriophages and bacterial isolates was extracted using QIAamp UltraSens Virus kit (Qiagen catalog number 53706) and DNeasy Blood & Tissue Kit (Qiagen catalog number 69504 ...
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bioRxiv - Systems Biology 2023Quote: Viral RNA was extracted from specimens using the QIAamp Viral RNA Minikit and the QIAamp 96 Virus QIAcube HT Kit (Qiagen). Viral transport media (VTM)-only controls were included in each extraction ...
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bioRxiv - Microbiology 2023Quote: DNase/RNase treated VLPs were used for genomic DNA extraction using Qiagen QIAamp DSP Virus Spin Kit (Qiagen, MD, U.S.A) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... round 1 and 3 virus infections of GeCKO-A549 cells using the midi gDNA extraction kit (Qiagen, Germantown, MD, USA). The sgRNA’s DNA copies were PCR amplified from the extracted gDNAs for next generation sequencing (Fig 1) ...
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bioRxiv - Microbiology 2023Quote: ... Nucleic acids were extracted from 140 µl of each sample and also from the original virus dilutions with the “QIamp viral RNA extraction kit” (Qiagen) and eluted in 60 µl elution buffer according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... A single colony was picked with an inoculation loop and DNA extraction was performed on the QIAsymphony using the DSP Virus/Pathogen Mini Kit (Qiagen). Library preparation performed using Nextera XT (Illumina Inc. ...
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bioRxiv - Microbiology 2024Quote: 200µL of lung of infectious cell supernatant (virus stock and HAE) was inactivated with an equal volume of VXL lysis buffer (Qiagen) and viral RNA was extracted using an EZ1 Advanced XL robot with the EZ1 mini virus 2.0 kit (both from Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... reverse transcription quantitative PCR (RT-qPCR) assays were performed with the QuantiTect Virus + ROX Vial Kit (Qiagen, Toronto, ON, Canada) in a LightCycler® 480 system (Roche Molecular System) ...
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bioRxiv - Cell Biology 2020Quote: ... Clarified lysate was mixed with Ni-NTA agarose at 4℃ for 2h (Qiagen). Bound protein was eluted in 20 mM Tris pH 8.0 ...
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bioRxiv - Developmental Biology 2021Quote: ... Lysates were treated with 4 μl of 100 mg/ml RNase A (QIAGEN) at 37 °C shaking at 500 RPM for 3 hours ...
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bioRxiv - Molecular Biology 2021Quote: ... The lysate was cleared by centrifugation before incubation on Ni2+ agarose beads (Qiagen) for 2h at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... and clarified lysate was incubated with 1 ml packed Ni-NTA resin (QIAGEN) per 750 ml culture for 1 hour at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... Clarified lysate was bound to pre-washed Ni-NTA agarose beads (Qiagen 1018236) in a gravity column and beads were washed with 10 column volumes lysis buffer ...
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bioRxiv - Bioengineering 2019Quote: ... The lysate was loaded into a column of Ni-NTA Agarose beads (Qiagen) and the protein was purified using increasing concentrations of imidazole ...
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bioRxiv - Biophysics 2019Quote: ... and RNA was isolated from cell lysates using the RNAeasy Mini Kit (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... the rest of lysate was incubated on a Ni-NTA agarose resin (QIAGEN) at 4 °C with rotation ...
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bioRxiv - Systems Biology 2020Quote: ... Homogenized organ lysates were then loaded onto the QIAshredder homogenizer spin columns (Qiagen) for further homogenization and elimination of insoluble debris ...
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bioRxiv - Molecular Biology 2019Quote: Total RNA was isolated from whole cell lysate using miRNeasy Mini Kit (Qiagen) then quantified using Qubit RNA HS Assay Kit (Life technologies) ...
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bioRxiv - Molecular Biology 2021Quote: ... The remaining lysates were incubated with nickel-nitriloacetic acid (Ni-NTA) beads (Qiagen) for 1.5 h at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA was purified from cell lysates using the RNAeasy Plus Kit (QIAGEN). RNA concentration and integrity were verified using high sensitive RNA Qubit reagent (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA was prepared from tissue lysates using a RNeasy 96 kit (Qiagen). The prepared RNA was assayed for MALAT1 and cyclophilin A levels using primer/TaqMan probe sets with an EXPRESS One-Step SuperScript quantitative reverse transcriptase polymerase chain reaction (qRT-PCR ...
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bioRxiv - Biochemistry 2021Quote: ... and fusion proteins were purified from clarified lysate through consecutive Ni-NTA (Qiagen), amylose (NEB ...
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bioRxiv - Microbiology 2020Quote: ... Cell lysate was mixed with 1 mL of Ni-NTA agarose beads (Qiagen), and the mixture was rotated at 4 °C for 2 hours ...
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bioRxiv - Microbiology 2020Quote: ... The clear solution containing lysate was passed through RNeasy mini column (Qiagen, Germany), which leads to the binding of RNA to the column ...
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bioRxiv - Zoology 2021Quote: ... Tissue lysates were further homogenized using a QIAshredder homogenizer (Qiagen, Germantown, MD, USA) to reduce viscosity and clarified by centrifugation (15000 g for 10 min at 10 °C) ...
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bioRxiv - Microbiology 2020Quote: ... The lysates were then incubated with 1 mL prewashed Ni-NTA resin (Qiagen) at 4 °C for 2 h on a rotator ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was isolated from bacterial lysate using RNeasy® Mini kit (Qiagen #74104) and eluted in sterile RNase-free water ...
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bioRxiv - Biochemistry 2022Quote: ... The cleared lysate was applied to a 5 mL Ni-NTA cartridge (Qiagen) and the column was washed in two steps with lysis buffer supplemented with 25 and 50 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... The cleared lysate was incubated with Ni-NTA agarose beads (Qiagen, Venlo, Netherlands) for 2 h ...
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bioRxiv - Microbiology 2022Quote: ... The cleared lysate was applied to a 5 mL Ni-NTA cartridge (Qiagen). The column was washed with 3 column volumes of lysis buffer and proteins were eluted stepwise with lysis buffer supplemented with 50 and 250 mM of imidazole ...
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bioRxiv - Immunology 2020Quote: ... RNA was extracted from the lysates using an RNeasy® Mini Kit (Qiagen), according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: A549 and primary hepatocyte cell lysates were homogenized over QIAshredder columns (Qiagen 79656) and RNA was extracted with the RNeasy mini kit (Qiagen 74106 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Protein was isolated from the lysate using 5 mL Ni-NTA resin (Qiagen) pre-equilibrated with lysis buffer and then washed with 15 column volumes (CV ...
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bioRxiv - Immunology 2022Quote: ... Total RNA was prepared from cell lysates using the RNeasy Mini Kit (Qiagen) and RNase-Free DNase Set (Qiagen ...
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bioRxiv - Immunology 2019Quote: ... RNA was isolated from the lysate by using the miRNeasy Micro Kit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: Total RNA was isolated from cell lysates using the RNeasy Micro Kit (Qiagen) including on-column DNase digestion ...