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Citations for Qiagen :
451 - 500 of 1718 citations for Dengue Virus serotype 2 lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... and RNA was purified from tissue lysate using the RNeasy Mini Kit (Qiagen) treated with DNase-I (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... and RNA from cell lysates was extracted using a RNeasy Mini Kit (Qiagen) as described previously (20 ...
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bioRxiv - Immunology 2020Quote: ... The clear solution containing lysate was passed through RNeasy mini column (Qiagen, Germany), which leads to the binding of RNA to the column.
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bioRxiv - Biochemistry 2021Quote: ... The cleared lysate was applied to a 5 mL Ni-NTA cartridge (Qiagen) and the column was washed in two steps with lysis buffer supplemented with 25 and 50 mM imidazole ...
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bioRxiv - Immunology 2021Quote: Total RNA was isolated from tissue lysates using the RNeasy Mini Kit (Qiagen), including on-column DNase digestion ...
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bioRxiv - Immunology 2021Quote: ... RNA was extracted from the tumor lysates with a RNeasy Mini kit (Qiagen) and was reverse transcribed with a First Strand cDNA synthesis kit (Fermentas) ...
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bioRxiv - Biochemistry 2022Quote: ... Clarified lysate was loaded on a 10 ml Ni-NTA Superflow column (QIAGEN), washed with 7 column volumes of 20 mM HEPES-KOH pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: ... Clarified lysate was loaded to a column prepared with Ni-NTA resin (Qiagen). Resin was washed with lysis buffer containing 60 mm imidazole and then protein was eluted with 400 mM imidazole ...
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bioRxiv - Evolutionary Biology 2022Quote: ... This lysate was used for RNA extraction with an RNeasy Mini Kit (Qiagen) according to the manufacturer’s standard protocol for bacterial RNA purification.
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bioRxiv - Microbiology 2022Quote: RNA was extracted from infected cell lysates using RNeasy® Mini Kit (QIAGEN) with an on-column DNase treatment (QIAGEN ...
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bioRxiv - Microbiology 2022Quote: ... Clarified lysate was passed over a Ni2+ affinity column (Ni-NTA Superflow, Qiagen) and eluted in a buffer with 250 mM imidazole ...
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bioRxiv - Microbiology 2022Quote: ... The resulting lysate was used as input for the RNeasy Mini kit (Qiagen) including an on-column DNase treatment (Qiagen) ...
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bioRxiv - Immunology 2022Quote: ... RNA was extracted from whole-cell lysates with RNeasy Plus Mini kit (Qiagen) and was reverse-transcribed with cDNA synthesis kit (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2022Quote: ... The cleared lysate was incubated with 0.25 ml of Ni-NTA beads (Qiagen) for 30 min ...
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bioRxiv - Microbiology 2023Quote: ... according to manufacturer’s instructions and lysates were homogenized through QiaShredder columns (Qiagen, Switzerland). The RNeasy Mini Kit (Qiagen ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein was isolated from the lysate using Ni-NTA Agarose resin (Qiagen) and was purified to homogeneity and >95% purity by size exclusion chromatography using a HiLoad Superdex 75 pg 16/600 column (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... The cleared lysate was incubated with pre-equilibrated Ni-NTA Agarose beads (Qiagen) for 4 h at 4°C ...
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bioRxiv - Neuroscience 2023Quote: TRAP and total lysate RNA were isolated via the RNeasy Plus Kit (Qiagen). The samples were first lysed and homogenized in Buffer RLT Plus ...
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bioRxiv - Developmental Biology 2023Quote: ... The lysates were column purified using QiaAMP DNA mini-Kit (Qiagen, Cat # 56304) and DNA quality of the eluate was determined using Nanodrop 2000 Spectrophotometer (#E112352 ...
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bioRxiv - Immunology 2023Quote: ... Total RNA was isolated from the lysate using miRNeasy Mini kit (Qiagen, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Total RNA was isolated from cell lysates using the RNeasy Micro Kit (QIAGEN), including on-column deoxyribonuclease digestion ...
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bioRxiv - Immunology 2023Quote: ... Total RNA was prepared from cell lysates using the RNeasy Mini Kit (Qiagen) and RNase-Free DNase Set (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... cell lysates were either mock-treated or treated with RNase A (Qiagen 19101) and then incubated at 4°C for 2 hours with biotinylated oligos ...
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bioRxiv - Biochemistry 2023Quote: ... The clarified lysate was applied to gravity flow Ni-NTA agarose resin (QIAGEN) previously equilibrated with wash buffer WB4_1 (50 mM Tris-HCl pH 8 ...
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bioRxiv - Microbiology 2023Quote: ... LdcAEc was purified from the cleared lysates via Ni-NTA agarose columns (Qiagen) and eluted with a discontinuous imidazole gradient using an ÄktaGo system ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was isolated and purified from the tissue lysates using miRNeasy columns (Qiagen) according to the an fact rer’s instr ctions ...
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bioRxiv - Biochemistry 2023Quote: ... Filtered cell lysate was applied to 10 mL of Ni-NTA resin (Qiagen) which had been previously equilibrated with 10 column volumes of H2O ...
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bioRxiv - Bioengineering 2023Quote: ... RNA extraction was performed from each cell lysate employing a miniprep kit (Qiagen). The RNA quality was analyzed using the 2100 Bioanalyzer (Agilent Technologies) ...
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bioRxiv - Cancer Biology 2023Quote: ... and DNA purified from lysate using a DNeasy Blood & Tissue Kit (QIAGEN, 69504) with slight modifications ...
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bioRxiv - Cell Biology 2023Quote: ... Cleared lysate was loaded on to 12ml of NiNTA agarose beads (Qiagen, 30250) and batchbound for 1.5 hours ...
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bioRxiv - Pathology 2024Quote: ... RNA was extracted from the lysate using the Qiagen RNeasy mini kit (Qiagen) according to the manufacturer’s instruction.
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bioRxiv - Microbiology 2021Quote: ... To generate a recombinant A_NS237 virus, viral RNA was extracted from allantoic fluid using Trizol reagent (Thermo Fischer, Germany) and Qiagen RNeasy Kit (Qiagen, Germany) following the manufacturers’ guidelines ...
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bioRxiv - Genomics 2020Quote: ... Viral RNA was extracted from 300 µl of viral transport media (VTM, Himedia, India) using QIAamp 96 Virus QIAcube HT Kit (Qiagen, Germany), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: A 200 μL aliquot of Copan UTM® was processed through the QIAsymphony DSP Virus/Pathogen Mini Kit (Qiagen, Cat# 937036) following manufacturer’s instructions on the QIAsymphony SP instrument ...
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bioRxiv - Molecular Biology 2020Quote: The RNA isolation was performed with 50 µl of the crude extract on the QIAsymphony with the DSP Virus/Pathogen Kit (Qiagen, #937055). The RT-PCR was performed using 5 µl of 85 µl eluate with TIB MolBiol Lightmix® MODULAR SARS AND WUHAN CoV E-Gene Kit ...
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bioRxiv - Genomics 2020Quote: Viral RNA was extracted from nasopharyngeal swabs of patients with COVID-19 using the EZ1 DSP Virus Kit (Qiagen, Hilden, Germany), optimized for viral and bacterial nucleic acids extractions from human specimens using magnetic bead technology ...
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bioRxiv - Microbiology 2021Quote: ... we collected the cell culture supernatants after 24 h of infection for viral RNA extraction with a QIAamp 96 Virus QIAcube HT Kit (Qiagen, 57731) and for viral RNA copy number detection in a CFX96 Touch Real-Time PCR Detection System (Bio-Rad Laboratories) ...
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from the lungs of influenza virus-infected mice using the RNeasy Mini Kit (QIAGEN, Cat. No 74004) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... RNAs from clarified lysates (60 μl) were isolated using the miRNeasy mini kit (Qiagen) according to manufacturer’s standard protocol ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... genomic DNA was isolated from tissue lysate with DNeasy Blood & Tissue kit (Qiagen, 69504). mtDNA copy number was determined by quantitative PCR with Human mitochondrial to nuclear DNA ratio kit (Takara Bio USA ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was isolated from tissue lysates using the RNeasy Mini Kit (cat# 74104, Qiagen).
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bioRxiv - Molecular Biology 2020Quote: ... The lysate was cleared and bound to pre-equilibrated Ni-NTA agarose beads (Qiagen) for 1.5 h ...
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bioRxiv - Genomics 2020Quote: ... gDNA was then purified from the lysate using the QIAamp DNA mini kit (Qiagen) according manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... The lysate was used for DNA isolation with Qiagen DNeasy Plant Mini Kit (Qiagen) following the manufacturer protocol ...
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bioRxiv - Cell Biology 2019Quote: ... The cleared lysate was loaded onto a pre-equilibrated Ni-NTA agarose column (Qiagen) and washed with buffer A supplemented with 30 mM imidazole ...
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bioRxiv - Cancer Biology 2020Quote: ... genomic DNA was isolated from cell lysates with a QIAamp DNA Mini Kit (Qiagen). Barcode sequences were amplified by PCR using 300 ng of genomic DNA (100,000-genome and 500,000-barcode equivalent ...
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bioRxiv - Biochemistry 2019Quote: ... The clarified lysate was first purified on a Ni-NTA column (Qiagen, Valencia, CA). The eluate was further purified on glutathione-Sepharose 4B resin (GE healthcare ...
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bioRxiv - Immunology 2021Quote: ... then clarified lysates were loaded onto a Ni2+ affinity column (Ni-NTA Superflow; Qiagen), washed in buffer A plus 300 mM NaCl and 20 mM imidazole pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... then clarified lysates were loaded onto a Ni2+ affinity column (Ni-NTA Superflow; Qiagen), washed in buffer A plus 300 mM NaCl and 20 mM imidazole pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... Clarified lysates were passed over 5 ml of packed Ni-NTA agarose resin (Qiagen) at 1.0 ml/min ...