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Citations for Qiagen :
351 - 400 of 1718 citations for Dengue Virus serotype 2 lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... RNA was then extracted from whole lysates using RNEasy kits (Qiagen, Inc.) and combined with mRNA capture beads ...
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bioRxiv - Biochemistry 2022Quote: ... The cleared lysate was applied to a Ni-NTA Superflow column (Qiagen). The column was washed with buffer A and a high salt buffer containing 50 mM Tris pH 7.2 ...
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bioRxiv - Microbiology 2022Quote: ... The lysate was incubated with 1 mL of Ni- NTA agarose (Qiagen) for 1 h at 25 °C with rotating ...
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bioRxiv - Molecular Biology 2022Quote: ... The clarified lysate was then loaded onto Ni- NTA spin columns (QIAgen) and proteins purified according to manufacturer protocol washing the column with Washing Buffer (30 mM Hepes pH 7.5 ...
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bioRxiv - Developmental Biology 2022Quote: ... and the lysate was spun through a QIAshredder column (Qiagen, cat#: 79656) to fully homogenize ...
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bioRxiv - Biochemistry 2022Quote: ... Clarified lysate supernatants were batch bound with equilibrated Ni-NTA resin (QIAGEN). Washes were performed with 5-10 column volumes of lysis buffer ...
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bioRxiv - Neuroscience 2023Quote: ... The lysate was then loaded onto a QIAshredder spin column (#79656, Qiagen) and placed in a 2 mL collection tube before being centrifuged for 2 minutes at full speed ...
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bioRxiv - Molecular Biology 2023Quote: ... total RNAs were extracted from lysates using a miRNeasy kit (QIAgen, #217004) following the manufacturer’s protocol for recovery of total RNAs (standard protocol) ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysate supernatants were applied to packed Ni-NTA beads (QIAGEN; 30210). After washing with 20 column volume of lysis buffer ...
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bioRxiv - Immunology 2023Quote: RNA from lysates was purified using RNeasy Plus RNA purification kit (Qiagen) and retrotranscribed using qScript cDNA supermix (Quanta bio) ...
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bioRxiv - Systems Biology 2023Quote: ... with the cell-lysate first being homogenized through a QIAshredder column (Qiagen), per the manufacturer’s directions ...
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bioRxiv - Bioengineering 2023Quote: ... RNA was isolated from the lysate with the RNeasy Mini Kit (Qiagen) according to the manufacturers protocol and quantified with NanoDrop 2000 (Thermo Scientific) ...
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bioRxiv - Immunology 2023Quote: RNA was extracted from LNSC lysates using the RNeasy Mini Kit (QIAGEN). The TaqMan® RNA-to-CT™ 1-Step Kit (Applied Biosystems ...
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bioRxiv - Biochemistry 2023Quote: ... The cleared lysate was loaded onto Ni-NTA Superflow resin (QIAGEN, 30410) equilibrated with lysis buffer and washed with 10 column volumes of washing buffer containing 20 mM Tris pH 8.0 ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was isolated from this lysate using the RNeasy mini kit (Qiagen). Next-generation sequencing was performed on these RNAs ...
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bioRxiv - Immunology 2023Quote: RNA was isolated from macrophage lysates (AllPrep DNA/RNA Micro Kit, Qiagen) and sequencing libraries prepared from 10ng RNA using the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Takara ...
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bioRxiv - Molecular Biology 2023Quote: ... and then passing the lysate through AllPrep DNA Mini Spin Columns (Qiagen) and proceeding with manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Thawed RLT lysates were then run over a QIAshredder column (Qiagen, 79654) and subsequently over a gDNA eliminator column ...
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bioRxiv - Cell Biology 2023Quote: ... The lysate was loaded onto three tandemly linked Strep-Tactin columns (Qiagen) and the complexes were eluted with 2.5 mM desthiobiotin (Sigma ...
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bioRxiv - Systems Biology 2023Quote: ... with the cell-lysate first being homogenized through a QIAshredder column (Qiagen), per the manufacturer’s directions ...
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Vps18 contributes to phagosome membrane integrity in Mycobacterium tuberculosis-infected macrophagesbioRxiv - Microbiology 2023Quote: ... RNA was isolated from the lysate using an RNEasy plus kit (Qiagen) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysate was subjected to genotyping using a HotStar Taq polymerase (QIAGEN) and previously established primers101 ...
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bioRxiv - Cell Biology 2023Quote: ... Subsequently RNA was extracted from tissue lysates using the RNeasy MiniKit (Qiagen) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was incubated for two hours with Ni-NTA beads (Qiagen). Non-specifically bound proteins were removed by washing the beads with 20 mM NaPO4 pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... The lysate was incubated with equilibrated Ni-NTA Agarose resins (Qiagen #30210) at 4ℒC for 2-4 hours ...
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bioRxiv - Cell Biology 2024Quote: ... RNA was purified from lysates using a RNeasy Plus Mini Kit (Qiagen). RNA-seq libraries and sequencing (RNA-seq ...
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bioRxiv - Immunology 2024Quote: ... RNA was extracted from tissue lysate by RNAEasy Mini column purification (Qiagen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and viral RNA was extracted using an EZ1 Advanced XL robot with the EZ1 mini virus 2.0 kit (both from Qiagen) and linear acrylamide (ThermoFisher Scientific ...
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bioRxiv - Epidemiology 2019Quote: Total RNA was extracted from SFTS virus IgM positive samples using a QIAamp viral RNA mini Kit (Qiagen, Germany), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: Viral RNA (vRNA) was isolated from virus-infected lung homogenate supernatant using the QIAamp Viral RNA Mini Kit (QIAGEN). Influenza genome copies were quantified from RNA isolations by reverse transcription and quantitative PCR (RT-qPCR ...
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bioRxiv - Microbiology 2021Quote: Virus isolation was performed on tissues following homogenization in 1 mL DMEM using a TissueLyser (Qiagen, Germantown, MD, USA) and inoculating Vero E6 cells in a 96 well plate with 200 µL of 1:10 serial dilutions of the homogenate ...
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bioRxiv - Microbiology 2020Quote: ... followed by extraction of viral nucleic acids using a commercial kit (QIAamp MinElute virus spin kit, Qiagen, Venlo, Netherlands). A portion (2.5 µl ...
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bioRxiv - Microbiology 2021Quote: ... the resulting filtrate was used for viral DNA extraction using the QiAmp Ultra-Sensitive Virus Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... Nucleic acid was first extracted from each blood sample using QIAamp MinElute Virus Spin kits (Qiagen, Mississauga, Ontario, Canada) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and subsequently extracted using the Qiagen EZ1 Advance XL extractor with the EZ1 Virus Mini Kit v2.0 (Qiagen, Germany). For the eastern Australian RSV cases ...
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bioRxiv - Microbiology 2020Quote: Viral RNA was extracted from 10,000 TCID50 WT and NS1R38A virus containing inoculum using the QIAamp Viral RNA mini kit (Qiagen), according to the manufactures protocol ...
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bioRxiv - Immunology 2021Quote: ... viral RNA was extracted from AFs stocks using the QIAamp MinElute Virus Spin kit (cat. n° 57704, Qiagen, Germany), according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Fold changes of N transcript expression with each treatment were calculated corcenning to the Virus control using REST 2009 program (v2.0.13, Qiagen, GbmH) [26] ...
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bioRxiv - Microbiology 2022Quote: ... Sample DNA was extracted from the viral enriched filtrate using the QiAmp Ultra-Sensitive Virus Kit (Qiagen, Hilden, Germany) and was subjected to whole genome amplification (WGA ...
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bioRxiv - Microbiology 2022Quote: ... followed by total nucleic acid extraction of 400 ul of pretreated stool using the EZ1 Virus Mini Kit v2.0 (Qiagen). Extracts were eluted in 60 ul volume.
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bioRxiv - Immunology 2022Quote: Serum viral RNA was extracted from 200 μl using the QiaAmp Min Elute Virus Spin kit (Qiagen, Cat# 57704) according the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Viral genomic RNA was purified from 280 μL virus-containing media using the QIAamp Viral RNA Mini Kit (Qiagen) as per the manufacturer’s instructions and eluted in 60 μL nuclease-free water ...
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bioRxiv - Microbiology 2023Quote: We extracted influenza virus genomic RNA from the clarified allantoic fluid using the QIAamp Viral RNA Mini Kit (Qiagen) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... and viral RNA was extracted using an EZ1 Advanced XL robot with the EZ1 mini virus 2.0 kit (both from Qiagen) and linear acrylamide (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... RNA from one aliquot per condition per virus isolate and negative control was immediately extracted with the QIAamp Viral RNA Mini Kit (Qiagen) and stored at −80°C until further processing ...
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bioRxiv - Microbiology 2021Quote: Total RNA of each isolate was extracted from frozen virus stock using QIAamp Viral RNA Mini Kit (Qiagen, Germantown, MD) following the manufacturers spin protocol ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted from the working stock of each reporter virus and treated with DNase using the DNase Max kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: 200µL of lung clarified homogenate or infectious cell supernatant (virus stock) was inactivated with an equal volume of VXL lysis buffer (Qiagen) and viral RNA was extracted using an EZ1 Advanced XL robot with the EZ1 mini virus 2.0 kit (both from Qiagen ...
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bioRxiv - Microbiology 2019Quote: ... RNA was extracted from 70 μL of supernatant using QIAamp 96 virus QIAcube HT kit on the QIAcube HT System (Qiagen). Each RNA sample was analyzed by one-step RT-qPCR with two SYBR Green assays ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA was extracted from supernatants of swab material using the QIAsymphony DSP Virus/Pathogen Mini Kit on the QIAsymphony instrument (Qiagen). RT-qPCR was then performed using the AgPath RT-PCR (Life Technologies ...