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Citations for Qiagen :
401 - 450 of 797 citations for Anion Exchange Membranes Thickness 10 13 µm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: ... We performed PCR in a 10 µL volume containing 1x multiplex PCR master mix (Qiagen Hilden, Germany), 5-20 ng of genomic DNA ...
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bioRxiv - Bioengineering 2020Quote: ... The ligated DNA was purified and concentrated into 10 μL dH2O via MinElute PCR Purification Kit (Qiagen). The plasmids were transformed into NEB 5-alpha Electrocompetent E ...
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bioRxiv - Biochemistry 2021Quote: Total RNA was extracted and DNAse treated from a 10-cm dish with RNeasy Kit (Qiagen, Germany), according to manufacture instruction ...
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bioRxiv - Biochemistry 2020Quote: ... cells were transfected in 10 cm2 or 6-well plates at ∼60% confluency using PolyFect (Qiagen 301107) and washed with complete media 6 h later.
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bioRxiv - Cancer Biology 2021Quote: Whole RNA (1-3 μg total per 10 μL volume) was isolated using RNAeasy mini kit (QIAGEN) or TRIzol (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... Cells were harvested by centrifugation for 10 min at 3200 x g and resuspended in RNAprotect (Qiagen). RNA was isolated using the RNeasy mini kit (Qiagen) ...
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bioRxiv - Neuroscience 2020Quote: ... the whole CNS was dissected from the snails (n=10) and homogenized using a TissueLyser LT (QIAGEN) in TRI reagent (#93289 ...
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bioRxiv - Molecular Biology 2021Quote: ... and ~10 mg was obtained for DNA isolation using the commercially available DNeasy Blood & Tissue Kit (Qiagen; Venlo ...
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bioRxiv - Genomics 2022Quote: ... The samples were cooled to room temperature and 30 μL of 10 mg/mL RNase A (QIAGEN) was added and the samples were incubated at 37°C for 1 hour ...
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bioRxiv - Developmental Biology 2022Quote: ... 8 to 10 independent PCR reactions were pooled together and purified using the PCR purification kit (Qiagen). Multiplexed libraries were sequenced on Illumina HiSeq 2500 to obtain 100 bp single-end reads ...
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bioRxiv - Microbiology 2022Quote: ... with the addition of a 10 min bead beating step at 50 s−1 (Qiagen, Hilden, Germany). RNA was isolated following the Ribopure-Bacteria kit protocol (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... The soluble fraction was purified using either 10 mL nickel-chelating column or Strep-Tactin column (Qiagen). The N-terminal His-SUMO tags were cleaved from the eluted protein by TEV protease overnight and removed by passing through a second nickel column ...
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bioRxiv - Plant Biology 2021Quote: ... genomic DNA (gDNA) was extracted from 10-day-old seedlings with the DNeasy plant mini kit (Qiagen) per manufacturer’s introduction ...
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bioRxiv - Neuroscience 2020Quote: ... 10 ng of bisulfite converted DNA was run in a PCR using the PyroMark PCR kit (Qiagen) and the following PCR primers ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 mM EDTA) followed by ethanol precipitation (<500 000 cells) or with QIAamp DNA Mini Kit (Qiagen) (>500 000 cells) ...
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bioRxiv - Immunology 2020Quote: ... Mechanical disruption of microbial cells was done using TissueLyser II (QIAGEN, 30 shakes/sec., 10-20 min.) and the samples were centrifuged (10,000 g ...
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bioRxiv - Immunology 2020Quote: ... in purity mode into 96-well microplates containing 10 μL of 1% 2-mercaptoethanol RLT buffer (Qiagen) and stored at −80°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and homogenized twice at 10 s at 2 M/s with a 5-mm steal bead (Qiagen) using a tissue homogenizer (MP Biomedicals) ...
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bioRxiv - Immunology 2021Quote: ... The samples (N=3) were collected immediately and soaked in 10 volumes of RNAlater (Qiagen, Hilden, Germany), for sequencing using Illumina (New England Biolabs) ...
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bioRxiv - Cancer Biology 2021Quote: ... 10 embryos were used for genomic DNA isolation using the Qiagen DNAeasy Blood & Tissue kit (Qiagen #69504). DNA was PCR amplified with Promega GoTag green mastermix (Promega #M7123) ...
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bioRxiv - Cell Biology 2020Quote: ... All 10 PCRs of a single sample were pooled and purified using QIAquick PCR Purification Kit (Qiagen). The purified PCR product was then separated using 2% agarose gel and gel extracted with the Qiagen Gel Extraction Kit ...
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bioRxiv - Genomics 2021Quote: ... 43 Banfora-S mosquitoes and 10 Banfora-O mosquitoes using a Qiagen DNeasy Blood & Tissue Kit (Qiagen) following manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... ∼ 10 mg of cryopreserved liver and spleen tissue samples were ground using a TissueRuptor (Qiagen, Hilden, Germany) in liquid nitrogen and subsequently homogenized in HB Buffer (see buffer recipes in Corces et al ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from ~ 10 g of thawed soil using Powermax Soil DNA extraction kit (Qiagen) with some minor modifications as follows ...
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bioRxiv - Biochemistry 2021Quote: Total RNA was isolated from 10 homogenized zebrafish embryos per sample using the RNeasy Mini Kit (Qiagen). RNA concentration was measured on a spectrophotometer (DeNovix DS-11 FX+) ...
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bioRxiv - Genetics 2020Quote: ... cell pellets (10*106 cells) were collected and RNA was extracted using the RNeasy Mini Kit (Qiagen). RT-PCR was performed using SuperScriptIII Reverse Transcriptase (ThermoFisher ...
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bioRxiv - Biochemistry 2021Quote: ... the sample was supplemented with 10 mM imidazole and incubated with 1 ml Ni-NTA resin (Qiagen) for 1 hour at 4 °C ...
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bioRxiv - Genomics 2021Quote: ... in a 20 μL system with a mixture of 10 μL 2×SYBR Premix ExTaq (Qiagen, Germany), 2.0 μL diluted cDNA or double distilled water as a negative control ...
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bioRxiv - Cell Biology 2021Quote: ... and 10 mg/mL proteinase K) and DNA clean up (MiniElute reaction clean up kit, 28204, Qiagen). qPCR was performed using mPer2 and Wnt3a primers ...
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bioRxiv - Genomics 2022Quote: ... powdered liver (~10 mg) was resuspended in 900 μL QIAzol (RNeasy 96 Universal Tissue Kit, Qiagen, 74881) and homogenized at 25/s for 1 min at 4°C using a TissueLyser II (Qiagen ...
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bioRxiv - Genetics 2022Quote: ... of which 2.5 μL was used for 10 μL reactions using the RotorGene Q qPCR system (QIAGEN). Reactions were performed with 5 min at 95 °C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA was eluted in 45 µl of EB buffer (10 mM tris-hydrochloride, pH 8.0; QIAGEN, Netherlands) supplemented with 0.05% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 sections were stored in -80°C prior to RNA extraction using the Rneasy micro kit (Qiagen). RNA quality was assessed using a 5300 Fragment Analyzer (Agilent ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 mM Tris pH 8.5, 2 mM MgCl2, 0.5% NP40, 0.5% Tween20, 0.05 U/mL QIAGEN protease) and following the procedure described in (42) ...
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bioRxiv - Cancer Biology 2023Quote: ... were transfected with 10 nM Malat1 ENE-targeting (ASO) or control (Con) steric blocking ASOs (Exiqon, Qiagen) using Lipofectamine 2000 (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2023Quote: Approximately 10 mg of each frozen tissue sample was homogenized in 1ml QIAzol Lysis Reagent (79306, QIAGEN) using BeadBug 6 Microtube Homogenizer (D1036 ...
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bioRxiv - Molecular Biology 2023Quote: ... and genomic DNA from 10 SPF shrimp was isolated using the DNeasy blood and tissue kit (Qiagen). LAMP reactions were prepared as detailed in Table S2a and run at 60°C and 62°C (TSV and WSSV ...
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bioRxiv - Genomics 2023Quote: ... 10 CNP) from foot tissue were performed using DNeasyBlood and Tissue DNA extraction kit (Qiagen Cat.no. 69504) following the manufacturer’s recommendations (Elution in 110 μl elution buffer) ...
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bioRxiv - Microbiology 2023Quote: ... tissues collected were weighed and homogenized in PBS/10% FBS using the TissueLyser (Qiagen; Retsch, Haan, Germany). After clarification by centrifugation ...
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bioRxiv - Immunology 2024Quote: ... we transfected the 293T cells at 3 million cells/10 cm dish with Effectene transfection reagent (Qiagen) following manufacturer guidelines ...
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bioRxiv - Plant Biology 2024Quote: RNA was extracted from six 10-day-old soil-grown seedlings with the RNeasy Mini kit (Qiagen). First strand synthesis was carried out with 2ug total RNA using the Maxima cDNA kit according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... samples were lysed in a heating block at 56 °C for 10 minutes using AL Buffer (Qiagen) supplemented with 0.2 mg/mL Proteinase K (Qiagen) ...
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bioRxiv - Genetics 2024Quote: ... the reaction volume was doubled and contained: 10 µl of 2X EpiTect® MethyLight qPCR reagent (Qiagen), 2 μl of 25mM MgCl2 (Applied Biosystems) ...
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bioRxiv - Genetics 2020Quote: ... denaturation solution (0.2M NaOH), and wash buffer (10 mM Tris-acetate, pH 7.6) using the PyroMark Q96 Vacuum Workstation (QIAGEN). Purified DNA resuspended in annealing buffer (20 mM Tris-acetate pH7.6 ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA was extracted from the gut samples (7-10 guts/sample) using the RNeasy Mini kit (Qiagen) and the on-column DNase I treatment (79254 ...
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bioRxiv - Microbiology 2020Quote: Viral RNA was extracted from 10 µL of mouse sera using QIAamp® viral RNA Mini Kit (Qiagen) or about 20 mg of organs homogenised by Precellys Lysing Kit Hard tissue grinding (Bertin Technologies ...
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bioRxiv - Genomics 2021Quote: ... single cell directly into 2 µl lysis buffer (30 mM Tris-HCl [pH = 8.0], 10 mM NaCl, 0.2 µL Proteinase K [Qiagen, #19133] ...
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bioRxiv - Microbiology 2020Quote: ... with YFV-C (1-10) K8ac peptide were grown from a solution containing 0.1 M HEPES (pH 7.0) (QIAGEN), 30% PEG 6000 (QIAGEN ...
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bioRxiv - Microbiology 2020Quote: ... with YFV-C (1-10) K4ac/K8ac peptide were grown from a solution containing 0.1 M HEPES (pH 7.0) (QIAGEN), 30% PEG 6000 (QIAGEN ...
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bioRxiv - Physiology 2021Quote: Total liver RNA was extracted from n=10 mice using a RNeasy Plus mini kit (Qiagen, Hilden, Germany). Total gonadal adipose RNA was extracted using a modified Tri-reagent (Sigma-Aldrich ...