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Citations for Qiagen :
651 - 700 of 797 citations for Anion Exchange Membranes Thickness 10 13 µm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The first PCR was conducted in a 10 μL reaction volume per sample using the Multiplex PCR Plus Kit (Qiagen) with primers 515f/806r (Apprill et al. ...
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bioRxiv - Genomics 2024Quote: Genomic DNA was extracted from 30-70 mg of tissue or 5.0 x 10^6 cells for Source 1 cell lines using commercially available QIAGEN QIAamp Mini Kit (QIAGEN, 51304), following the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: Total RNA was isolated from 10 third-instar larvae for three biological replicates using an RNeasy Lipid Tissue Mini kit (Qiagen). DNA contamination was removed using TURBO DNA-free (Ambion) ...
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bioRxiv - Microbiology 2024Quote: ... BALF samples and 10% tissue homogenates were mixed with an equal volume of the RLT buffer (Qiagen, Germantown, MD, USA). Two hundreds microliter of the sample lysate and 200 µL isopropanol was added into the extraction plate ...
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bioRxiv - Microbiology 2024Quote: ... was labeled and stored at -80°C until DNA was extracted using the DNeasy PowerMax Soil kit (12988-10, Qiagen).
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bioRxiv - Microbiology 2024Quote: The pellet from the first Moore swab tube (T1) was extracted using the DNeasy PowerSoil Pro Kit (12988-10, Qiagen) following the manufacturer’s protocol with the following modifications ...
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bioRxiv - Microbiology 2024Quote: All soil samples and the wastewater sediment samples were processed using the DNeasy PowerMax Soil kit (Qiagen cat # 12988-10). The DNeasy PowerWater kit (Qiagen cat # 14900- 100-NF ...
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bioRxiv - Immunology 2024Quote: Total RNA was isolated from CD4+Foxp3YFP+ Tregs and CD4+Foxp3YFP− Tcons from WT-Foxp3YFPCre/YFPCre and Dgka-/-zfl/fl-Foxp3YFPCre/YFPCremice (10–12-week-old) using the RNeasy Plus Mini kit (Qiagen). RNA-sequencing libraries were prepared using Truseq stranded mRNA kit (Illumina ...
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bioRxiv - Immunology 2024Quote: Biopsies (n= 10 from non-IBD controls, n= 29 biopsies from 15 UC patients) were placed in RNA later (Qiagen) and stored at −80°C until further analysis ...
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bioRxiv - Immunology 2024Quote: ... RNA extractions were performed on 10 mg (bladder) or 20 mg (decidua, placenta) by RNeasy Plus Mini Kit (Qiagen 74134). RNA sequencing was then performed by Novogene ...
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bioRxiv - Plant Biology 2024Quote: ... tissue was ground with liquid nitrogen using a mortar and pestle and suspended in 10 mL of QIAIzol reagent (Qiagen). This suspension was separated via centrifugation (10 minutes at 4,000g ...
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bioRxiv - Microbiology 2024Quote: ... eggs and plant pellets were transferred into the lysis buffer in the Powerbead tubes and vortexed (10 min, 50 Hz) with TissueLyser LT (QIAGEN). DNA was eluted in 40 µL Nuclease Free Water (Thermo Fisher Scientific Inc. ...
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bioRxiv - Molecular Biology 2024Quote: ... To assess ribosomal biogenesis total RNA was extracted from ∼10 mg of heart tissue using Qiagen RNeasy Fibrous Tissue Mini Kit (Qiagen). Concentration and quality of isolated RNA were determined by NanoDrop (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: Collected microvessels were pelleted at 20,000 × g for 10 min and resuspended in 350 μl of RLT Plus Buffer from the RNeasy Micro Kit (QIAGEN). RNA was isolated using the RNeasy Plus Micro Kit ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was filtered using a 0.22 μm syringe filter and loaded onto a NiNTA 10/50 column (Qiagen, Germany) and proteins were eluted with an increasing gradient of imidazole ...
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bioRxiv - Genetics 2024Quote: ... DNA samples (70–80 oocytes and 7–10 blastocysts per sample) were subjected to bisulfite conversion through 2 µg carrier RNA (QIAGEN). Nested PCR was performed using EpiTaq HS with the primers listed in Supplementary Table 4 ...
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bioRxiv - Plant Biology 2024Quote: ... Genomic DNA was isolated from 10-day-old L19 mutant seedlings by using a DNeasy Plant Mini Kit (Qiagen, www.qiagen.com) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... total RNA was extracted from the anterior half of the body from 10-12 5 dpf larvae per sample at using the Rneasy Mini Kit (Qiagen). cDNA was synthesized using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) ...
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bioRxiv - Genomics 2024Quote: Ten aphids (n = 10 × 4) were ground to powder with a liquid nitrogen-cooled micropestle after which the RNeasy kit (Qiagen) was used for RNA extraction with on-column DNA digestion (Qiagen) ...
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bioRxiv - Biochemistry 2024Quote: ... DNase digestion was performed by adding 80 µL of DNase solution (10 µL RNase-free DNase I stock (Qiagen #79254) plus 70 µL Buffer RDD (Qiagen #79254) ...
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bioRxiv - Zoology 2024Quote: ... Multiplex PCR was performed in 10 µl reaction volume containing 5 µl 2x QIAGEN Multiplex PCR Master Mix (QIAGEN, Germany), 2 µl 5x Q-Solution (QIAGEN) ...
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bioRxiv - Cancer Biology 2024Quote: ... Lysate was incubated for 10 min on ice and nucleic acids were isolated using the AllPrep micro kit (Qiagen # 80204) as described in Scherer et al.39 RNA and DNA concentrations were determined by Qubit broad range kits (Thermo Fisher #Q10211 ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was isolated from mouse prostate cell lines or WT prostate tissue from 8-10 week old C57BL/6 mice using the RNeasy Mini Kit (Qiagen). Complementary DNA (cDNA ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were quickly weighed and ice-cold PBS supplemented with the protease inhibitor cocktail was added to each sample to make 10 % homogenate while using TissueLyser II (Qiagen) with one stainless steel bead (5 mm ...
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bioRxiv - Zoology 2021Quote: ... PCR amplifications were carried out in 10 μl reaction volumes containing 5 μl Type-it Microsatellite PCR Kit (Qiagen, Hilden, Germany), 0.5 μM of each primer ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA was extracted and 5 μg reverse transcribed from paired isolated Jz and Lz placental tissues (n = 8-10 per genotype/sex, across 11 litters) using the RNeasy Plus Mini Kit (Qiagen, DE) and the High-Capacity cDNA Reverse Transcription Kit minus RT inhibitor (Applied Biosystems ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Multiplex amplification was carried out in 10 μl reaction volumes containing 5 μl of QIAGEN Multiplex PCR Buffer Mix (QIAGEN Inc.), 0.2 μM labeled forward primer (Applied Biosystems) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Total RNA was extracted from 10 samples (5♀♀, 5♂♂) using RNeasy Micro kits (QIAGEN K.K., Tokyo, Japan) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... 0.5 µl of diluted template cDNA (1:3, nuclease-free water) per real-time PCR reaction (10 µl – SYBR Green PCR kit, Qiagen, 204143) was used to assay specific transcript abundance (CFX96 Real-Time System ...
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bioRxiv - Cancer Biology 2022Quote: Genomic DNA (gDNA) was extracted from snap-frozen tissues (∼10–20 mg) using DNeasy Blood and Tissue Kit (Qiagen, Cat# 69504) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Naso- and oropharyngeal swab samples were centrifuged at 17,000xg for 10 mins at 4°C to obtain cell pellets which were washed once using PBS and lysed by bead-vortexing for 10 minutes in cell lysis buffer (RLT, Qiagen, Germany). Proteins (as well as DNA and RNA ...
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bioRxiv - Immunology 2020Quote: Airway samples were centrifuged at 17,000xg for 10 mins at 4°C to obtain cell pellets which were washed once using PBS and lysed by bead-vortexing for 10 minutes in cell lysis buffer (RLT, Qiagen, Germany). Proteins (as well as DNA and RNA ...
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bioRxiv - Biochemistry 2020Quote: ... was mixed with selection plasmid pCoBlast (1:10) and the mixture was used for cells transfection by Effectene Transfection Reagent (QIAGEN, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... total RNA from the fibroblasts cultured with 10 μM Dwn1 was extracted every 6 hours from hours 24 to 48 (RNeasy® Plus Mini kit, Qiagen), followed by cDNA synthesis (SuperScript® VILO™ cDNA Synthesis Kit ...
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bioRxiv - Microbiology 2020Quote: ... Each 10 μL uPCR reaction contained 2 μL of DNA template with 1x QuantiTect Multiplex PCR No ROX mastermix (Qiagen™), 0.4 μM each primer ...
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bioRxiv - Neuroscience 2020Quote: DNA and RNA from 8-10 mice was isolated and purified with AllPrep-DNA/RNA/miRNA-universal kit (Qiagen, Montreal, QC) and concentrations were determined by fluorometry on the Qubit system (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: Cells debris was pelleted by centrifugation at 23,000 x g for 45 minutes and the supernatant loaded onto a 10 ml Ni2+-NTA column (Qiagen, Hilden, Germany). The column was washed with 600 ml buffer A and protein batch eluted with 100 ml buffer B (buffer B = buffer A supplemented with 290 mM imidazole).
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bioRxiv - Neuroscience 2020Quote: ... The high-quality reads were de novo assembled using the short reads assembly program Trinity version 2.3.2ref56 as well as a CLC Genomics Workbench version 10 (Qiagen, Carlsbad, CA, USA). The Trinity assembly yielded a total of 74,151 predicted ‘genes’ with their isoforms totaling 171,567 predicted ‘transcripts’ (i.e. ...
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bioRxiv - Evolutionary Biology 2022Quote: ... 10-20 mg of silica-dried stem or leaf tissue was ground with a Qiagen TissueLyser II (Qiagen, Melbourne, Vic., Australia). DNA was extracted using the DNeasy 96 Plant Kit or DNeasyPlant Mini Kit (Qiagen ...
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bioRxiv - Immunology 2022Quote: ... 65-79*SE or 65-79*PE (100 μg/mL) with or without IFN-γ (10 ng/mL) using the RNeasy mini kit (Qiagen) according to the vendor instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The coverslips were then incubated for ~ 10 min with 4 μg/ml of Penta•His biotin conjugate antibody (34440, Qiagen, UK) in reaction buffer [40 mM HEPES buffer (pH 7.3 ...
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bioRxiv - Immunology 2021Quote: At least 10,000 sorted cells or 10 μL of whole blood sample were collected for genomic DNA extraction using the DNeasy Blood & Tissue Kit (Qiagen, #69504). 3 μL of genomic DNA was used as template to amplify the sgRNA target region ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Total RNA was extracted from 10 samples (5♀♀, 5♂♂) using RNeasy Micro kits (QIAGEN K.K., Tokyo, Japan) following the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... The sample was then eluted in buffer AE (10 mM Tris-Cl; 0.5 mM EDTA; pH 9.0; Qiagen Inc., Courtaboeuf, France). DNA purity was assessed with a NanoDrop spectrophotometer based on the 260/280 and 260/230 O.D ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The aqueous fraction of the Trizol lysate was precipitated with ethanol and was resuspended in 10 mM Tris pH 8.0 before purification using the QIAamp viral RNA mini kit (QIAGEN, Manchester, UK) in the absence of carrier RNA ...
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bioRxiv - Microbiology 2021Quote: ... 1.5mL of the culture was pelleted (13500 rpm for 10 min) and used for the extraction of DNA with DNeasy Blood and Tissue Kit (Qiagen, Germany). For eluting nucleic acids ...
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bioRxiv - Genomics 2020Quote: BAC clones were extracted from each of these primary pools using either the Qiagen Large-construct Kit (10) (12462, Qiagen, Germany) or Omega BAC/PAC DNA Maxi Kit (D2154-02 ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted from MCF10AER/vSrc cells (Control and treated with 10 nM D-Alanine for 4 h or 24 h) using RNeasy Kit (Qiagen, #74104) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: DNA was extracted from 1×10^6 CD4 Th cells/ replicate using the Qiagen DNA mini kit according to manufacturer’s instructions (Qiagen Hilden, Germany). DNA was extracted from 10ng of mouse brain tissue as previously described13 using NEB Monarch High molecular weight DNA extraction kit according to manufacturer’s instructions (NEB Cat #T3050).
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bioRxiv - Plant Biology 2022Quote: ... pods from 8-10 different plants were pooled and RNA was isolated using RNAeasy plant mini kit (Qiagen, Germantown, MD, USA). RNA libraries were prepared using standard Illumina protocols and RNA sequencing was performed using NovaSeq 6000 PE150 by Novogene co ...