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Citations for Qiagen :
551 - 600 of 797 citations for Anion Exchange Membranes Thickness 10 13 µm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2020Quote: ... DNA was extracted from homogenized whole bodies with a Qiagen DNeasy kit after overnight treatment with 10% proteinase K (Qiagen) at 56 °C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The tissue was ground to a fine powder by adding 10-15 zirconia beads (1 mm diameter) and using a TissueLyser (Qiagen) for sunflower ligules ...
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bioRxiv - Molecular Biology 2020Quote: ... after adding 1.0 x 10^8 copies/μL of cel-miR-39 exogenous spike-in control (Qiagen 219610 Lot#157036035), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: DNA and RNA were extracted from the cervical cancer tissues (5-10 mg) using the AllPrep DNA/RNA Micro Kit (QIAGEN) as described by the manufacturer ...
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bioRxiv - Immunology 2020Quote: ... and DNA from 7-10 clones from before and after FLPo-mediated recombination was prepared by miniprep (Qiagen, cat. 27106) and sequenced by Sanger sequencing with the primer GFP int F Age 66 ...
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bioRxiv - Genomics 2021Quote: ... Nuclei pellets were centrifuged for 10 min at 1500g and processed immediately for gDNA extraction using a QIAamp DNA micro kit (Qiagen) according to manufacturer instructions.
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bioRxiv - Cancer Biology 2021Quote: ... Cells attached to the collagen matrix were fixed with 4% PFA for 10 minutes at room temperature for IF analysis or lysed with RLT buffer (Qiagen) for RNA isolation and qRT-PCR analysis as described above.
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bioRxiv - Biophysics 2021Quote: ... PNGase F and the cleaved 10 × His tag were removed by passing the sample through Ni-NTA superflow resin (QIAGEN). The receptor was concentrated to 20–30 mg/ml with a 100 kDa cut-off concentrator (Millipore) ...
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Oligomerization state of the functional bacterial twin arginine translocation (Tat) receptor complexbioRxiv - Biophysics 2021Quote: ... The supernatant was loaded onto a 10 x 1 cm column with 2 mL Ni-NTA Superflow resin (Cat. #30230, Qiagen) that was pre-equilibrated with Buffer A (10 mM CAPS ...
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bioRxiv - Plant Biology 2021Quote: ... Fluorescence positive cells were collected in a round-bottom polystylene tube that contains 150 - 200 μl RLT buffer with beta-mercaptoethanol (10 μl / 1 ml RLT buffer, RNeasy micro kit protocol, Qiagen). Cell sorting was performed for about 15 min and collected cells were immediately frozen on dry ice and kept in −80 °C for up to one week ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA was extracted from cells collected from 10 mL late-exponential phase culture in Todd-Hewitt broth with the DNeasy Blood & Tissue kit (Qiagen) using a modified protocol with an additional lysis step as described previously 61 ...
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bioRxiv - Cell Biology 2021Quote: ... cells were seeded at 50% confluency in a 10 cm dish and transfected with 200 pmol siRNA (Qiagen, Germantown, MD) using RNAiMax Lipofectamine (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... Two days after infection the mice were sacrificed and the distal 10 cm of the small intestine was dissected and shortly incubated in RNA later (Qiagen). Then the tissue was flushed with ice-cold DPBS to remove the fecal content and opened longitudinally on ice using scissors ...
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bioRxiv - Genomics 2021Quote: ... a 1.0 μL aliquot of each sample was amplified in 10 μL reactions incubated at 30°C for 16 h as described in the REPLI-g Mini Kit (QIAGEN).
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from vpr+ and vpr- cells 10 days post-infection using Qiagen DNeasy Blood & Tissue kit (Qiagen) and 200 ng of DNA was sheared to 1 kb fragments using Covaris M220 and micro- TUBE according to the manufacturer’s recommended settings (Covaris ...
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bioRxiv - Cell Biology 2021Quote: RNA was isolated from cells (6-well dishes) or liver pieces (∼10-15mg) using the RNeasy Plus Mini kit (Qiagen). For livers ...
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bioRxiv - Cancer Biology 2021Quote: RNA from infected cell lysates was harvested by incubating infected Vero E6 cells for 10 minutes with RLT buffer (Qiagen) containing 2-Mercaptoethanol (Fisher Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... and 10 % (v/v) glycerol) for 30 s at a frequency of 15 s-1 using a TissueLyser II (QIAGEN). As the tissue homogenates were to be used for both native and SDS PAGE ...
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bioRxiv - Cancer Biology 2022Quote: DNA was extracted from 10 micro-meter slices using the Qiagen GeneRead DNA FFPE extraction kit (Qiagen, Catalogue no. 180134) which includes a Uracil N Glycosylase enzyme treatment to reduce FFPE artefacts ...
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bioRxiv - Biochemistry 2022Quote: ... were purified with 25 μL of Mag-bind Total Pure NGS beads and normalised in the same step by taking 10 μL of each reaction after resuspension (Qiagen Elution buffer ...
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bioRxiv - Microbiology 2022Quote: ... cells were lysed in TE buffer (pH=8) supplemented with 1 mg/ml of lysozyme and 10 µl of proteinase K (Qiagen) for 10 minutes at room temperature with a shaking of 500 rpm ...
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bioRxiv - Microbiology 2022Quote: Genomic DNA was extracted from approximately 10 g of sediments for each depth using PowerMax Soil DNA Isolation Kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA was extracted from pooled (n=10) embryonic zebrafish and pooled (n=3) adult (> 1 year) heart samples by QIAGEN RNEasy Lipid Tissue Extraction kit according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: RNA was extracted from clarified cell culture supernatants (16,000 x g for 10 min) and infected cells using a QIAamp Viral RNA MiniKit and RNeasy Plus mini kit (Qiagen), respectively ...
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bioRxiv - Cell Biology 2021Quote: ... 10∼12 ml cell culture medium was subjected to extracellular RNA isolation using the exoRNeasy Maxi kit (Qiagen, Cat# 77164) with minor modifications ...
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bioRxiv - Microbiology 2022Quote: ... with heat inactivation at 75°C for 10 minutes and DNA was further removed using a RNeasy Mini kit (Qiagen). RNA was harvested from three independent cultures as biological replicates per experiment ...
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bioRxiv - Immunology 2022Quote: Total RNA was extracted from cryopreserved PBMCs at 7 different time-points (10, 33, 56, 94, 120, 185, and 198 wpi) using the AllPrep DNA/RNA mini kit (Qiagen) as per the manufacturer’s specifications ...
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bioRxiv - Microbiology 2022Quote: For RNAseq the pellets were resuspended in 150 µL 10 mM Tris-HCl pH 8 and mixed with 700 µL of ice cold RLT+BME (RLT buffer (Qiagen) supplemented with 1% β-mercaptoethanol ...
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bioRxiv - Cancer Biology 2022Quote: ... Red blood cells were eliminated by incubation for 10 min on ice with 5 mL of erythrocyte lysis buffer (Qiagen). Human cells were enriched using the Mouse Cell Depletion Kit and the QuadroMACS magnetic cell separator (Miltenyi Biotec ...
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bioRxiv - Biochemistry 2022Quote: ... as previously described.10 Detection of 6x His-tagged recombinant POLIB variants was performed with primary antibody Penta•His (1:1000, Qiagen) for one hour ...
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bioRxiv - Immunology 2020Quote: Genomic DNA was extracted from 1×10^7 PBMCs using the AllPrep DNA/RNA/miRNA Universal Kit (Qiagen, Hilden, Germany). Following nucleic acid isolation ...
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bioRxiv - Neuroscience 2020Quote: ... Frozen right hemibrain was crunched in liquid nitrogen and 10-20mg of pulverized mouse brain was used for RNA extraction with RNeasy Plus Mini Kit (Qiagen). RNA concentration and quality were determined using Agilent RNA 6000 Nano Kit according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... Glass beads (3mm) and 1 mL of sterile water were added for homogenization at 30Hz for 10 minutes (TissueLyser II, Qiagen). Homogenates were diluted with PBS and inoculated onto SAB plates and incubated for 3 days at 30°C ...
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bioRxiv - Microbiology 2021Quote: ... samples were weighed and homogenized in DMEM containing 10% FBS and 1% antibiotics using 5 mm stainless steel beads (Qiagen) and the TissueLyser II (Qiagen) ...
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bioRxiv - Bioengineering 2021Quote: ... and 10 mM imidazole was added to the supernatant then loaded to a gravity column packed with Ni-NTA resins (Qiagen) pre-equilibrated with 20 mM Tris ...
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bioRxiv - Immunology 2021Quote: ... Cells were then centrifuged at 300 x g for 10 minutes and vigorously resuspended in 350µL RLT Buffer (Qiagen, Germany) by vortexing for 30 seconds ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA was extracted from ≈10 mg of MCT rat lung tissues using the RNeasy Fibrous Tissue Mini Kit (74704, Qiagen) following to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: RNA was extracted using TRIzol according to the manufacturer’s instructions and contaminating DNA was removed by DNase treatment for 10 mins and column purification (Qiagen RNAeasy). cDNA was prepared from 1µg total RNA using qScript reverse transcription kit (Quantabio ...
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bioRxiv - Cell Biology 2021Quote: ... 10 μm thick CCO/SDH-stained sections and DNA was extracted using the QIAamp DNA Micro Kit (Qiagen, Cat # 56304), using 2 μL of carrier RNA to enhance DNA yield ...
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bioRxiv - Biochemistry 2020Quote: ... 80 µL of the mixture was then incubated for 45-60 minutes at room temperature or 4°C with 10 µL NiNTA resin (Qiagen) prewashed with His-SUMO Lysis buffer (20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... The clarified extract was supplemented with 10 mM imidazole and rotated for 2 hours at 4 °C with 1 ml of Ni-NTA beads (Qiagen). Bound protein was eluted with 10 CV of buffer L / 200 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: The cells of each positive clone were collected by centrifugation (400 × g for 10 min at room temperature) and the RNA extracted with the commercial kit RNeasy Mini (Qiagen), following the protocol established by the manufacturer ...
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bioRxiv - Biochemistry 2021Quote: ... The Bacmid DNA pellet was then dried for 1 minute and re-suspended in 50 μL Buffer EB (10 mM Tris-Cl, pH 8.5; All buffers from QIAprep Spin Miniprep Kit, Qiagen #27104). Purified bacmid was then transfected into Sf9 cells ...
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bioRxiv - Genomics 2021Quote: We collected high molecular weight genomic DNA from whole blood or tissue from 36 coyotes sampled from Louisiana and 10 red wolves from North Carolina using the DNeasy Blood and Tissue Kit (Qiagen) and followed the manufacturer’s protocol for mammals ...
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bioRxiv - Plant Biology 2021Quote: ... genomic DNA (gDNA) was extracted from 10-day-old 2C and 4C rice seedlings with the DNeasy plant mini kit (Qiagen) per manufacturer’s introduction ...
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bioRxiv - Plant Biology 2021Quote: Total RNAs were extracted from 10-day-old 2C and 4C rice seedlings using the RNeasy plant mini kit (Qiagen). cDNA library construction and sequencing were carried out by Beijing Genomics Institute (BGI ...
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bioRxiv - Cell Biology 2020Quote: ... RNA was further digested with TURBO DNase overnight at 37°C (10 U per 2 μg of RNA) and purified with the RNeasy MinElute Cleanup Kit (Qiagen) following the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... cells were either left untreated or treated with 1000 IU/ml recombinant IFN-α treatment and incubated for 6 h or transfected overnight with polyI:C (10 μg/ml) using Polyfect (Qiagen). Luciferase assays were carried out ...
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bioRxiv - Genomics 2020Quote: ... 25 nuclei were sorted into each well of 96-well plates (8-10 plates per experiment) containing 12 µl of nuclear lysis buffer (11 µl of EB buffer (Qiagen) supplemented with 0.5 µl of 100X BSA and 0.5 µl of 1% SDS) ...
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bioRxiv - Immunology 2021Quote: THP-1 human monocytes or mature primary human peripheral blood derived macrophages were transfected with control (D-001810-10-05, Dharmacon) or SP140 (L-016508-00-0005, Dharmacon) SMARTpool siRNA (100nM) using HiPerfect reagent (Qiagen) or RNAiMAX (Invitrogen ...