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Citations for Qiagen :
451 - 500 of 797 citations for Anion Exchange Membranes Thickness 10 13 µm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... and adjacent normal tissues from 10 ICC patients were isolated using a RNeasy Mini Kit (Qiagen, Hilden, Germany). Strand-specific libraries were prepared using a TruSeq Stranded Total RNA Sample Preparation kit (Illumina ...
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bioRxiv - Plant Biology 2020Quote: ... Roots were washed quickly (< 1 min) to detach the surrounding peat matrix using 10% RNAlater (QIAGEN, Valencia, CA) in 1X PBS as the capture buffer to mitigate RNA degradation ...
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bioRxiv - Cell Biology 2021Quote: Total RNA was extracted from 10-day-old Arabidopsis seedlings with an RNeasy® Plant Mini Kit (QIAGEN) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... the platelets were pelleted at 1000 x g for 10 minutes in the presence of prostacyclin (PGI2, 2μM) and re-suspended in 500μl of Qiazol (QIAGen).
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bioRxiv - Genomics 2021Quote: ... Nuclear DNA was extracted individually from 10 mg of tissue using the DNeasy Plant Mini Kit (QIAGEN, DE) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... approximately 10 mg (wet weight) of both harvested cultures were homogenized by using a TissueLyser LT mill (Qiagen) and extraction was performed by chloroform and methanol solution (ratio – 2:1 ...
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bioRxiv - Genomics 2020Quote: ... Individual cells were sorted into the wells of 96-well plates with 10 μL TCL buffer (Qiagen, #1070498) with 1% beta-mercaptoethanol (Sigma 63689 ...
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bioRxiv - Microbiology 2023Quote: ... A 5 ml aliquot of culture was removed and added to 10 ml of RNAprotect Bacteria Reagent (Qiagen) and mixed by vortexing ...
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bioRxiv - Neuroscience 2023Quote: DNA was extracted from buccal cells collected age 6 (M=6.09, SD=0.24) and at age 10 (M=10.09, SD=0.29) using QIAamp DNA Mini Kit (Qiagen, Germany). The Infinium MethylationEPIC array (Illumina ...
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bioRxiv - Neuroscience 2023Quote: DNA was extracted from buccal epithelial cells collected at age 6 (M=6.09, SD=0.24) and at age 10 (M=10.09, SD=0.29) using QIAamp DNA Mini Kit (Qiagen, Germany), and was quantified using Quant-iT PicoGreen reagent (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Genomic DNA was extracted from 10 mg homogenized fresh frozen tumors using DNeasy® Blood & tissue kit (Qiagen). Subsequently ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted from 5-10 g of soil per sample with the DNeasy PowerMax Soil Kit (Qiagen) and used for short read and long read sequencing ...
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bioRxiv - Molecular Biology 2022Quote: Whole RNA was extracted from over 1 × 10^5 cells using the QIAGEN RNeasy Micro kit (QIAGEN, 74004) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Total RNA was extracted from 50 μl of 10% suspension using an RNeasy Plus kit (Qiagen, Hilden, Germany). For PCR-positive samples ...
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bioRxiv - Neuroscience 2024Quote: RNA from immunopanned astrocytes or neurons from 10-month hCOs was isolated using RNAeasy Micro Kit (Qiagen, 74004). Bulk RNA sequencing libraries were prepared using the NEBNext Ultra II kit with PolyA Selection and sequenced using the Illumina HiSeq 4000 System to a minimum of 30 million paired-end reads per sample ...
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bioRxiv - Systems Biology 2023Quote: ... 30,000 transformants was pooled and purified by splitting the entire pellet over 10 Miniprep columns (QIAGEN, Hilden, Germany) and eluting each in 30 μl of EB Buffer ...
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bioRxiv - Physiology 2023Quote: RNA was extracted from 10-15 mg of powdered frozen heart tissue (RNeasy Fibrous Tissue Mini Kit, Qiagen) and used for cDNA synthesis (QuantiTect Reverse Transcription Kit ...
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bioRxiv - Molecular Biology 2023Quote: ... genomic DNA from 10 adult flies (5 males/5 females) was extracted using DNeasy Blood & Tissue Kit (Qiagen). DNA was resuspended and sheared in 1X TE (0.1 mM EDTA ...
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bioRxiv - Neuroscience 2022Quote: ... canonical pathways were generated through the use of Ingenuity Pathway Analysis (IPA, Release Date: 2021-10-22, Qiagen).
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA was extracted from pooled individuals (n = 10) using a DNeasy® Blood & Tissue kit (Qiagen, Hilden, Germany). A continuous long-read library was prepared and sequenced using PacBio Sequel II technology by Berry Genomics (Berry Genomics Co ...
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bioRxiv - Microbiology 2022Quote: RNA was extracted from 5-10 snap-frozen larvae with the RNeasy Mini kit (Qiagen cat no. 74104) and reverse-transcribed using QuantiTect Reverse Transcription kit (Qiagen cat no 205311 ...
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bioRxiv - Molecular Biology 2022Quote: ... Up to 10 consecutive slides per patient were micro-dissected and collected in 350µl of RLT buffer (Qiagen). The sample was inverted and centrifuged at 13.000rpm for 5 min and stored at −80°C ...
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bioRxiv - Genomics 2022Quote: ... DNA fragments were released by addition of 15 µL 10% SDS and 30 µL Proteinase K (Qiagen, #19133) to each tube followed by incubation at 50°C for 3h ...
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bioRxiv - Cell Biology 2023Quote: ... and transfected with 10 ng/well of dsRNA targeting the candidates using the Effectene Transfection reagent (301427, QIAGEN). dsCtl and Dynasore (D7693 ...
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bioRxiv - Cancer Biology 2023Quote: ... Total cell-free DNA from urine supernatants was extracted from a median of 3.6 mL urine supernatant (range 0.7–3.65 mL) incubated with 10% ATL buffer before purification using the QIAsymphony DSP Circulating DNA Kit (Qiagen).
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bioRxiv - Cell Biology 2023Quote: ... 10 minutes 4°C and the supernatant was incubated with pre-equilibrated Ni-NTA agarose beads (Qiagen, #1018244) with shaking on ice for 3 hours ...
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bioRxiv - Microbiology 2024Quote: Genomic DNA from 10 ml Enterococci overnight cultures was isolated using the Qiagen Genomic-tip 100/G (Qiagen) Kit according to the manual for gram positive bacteria ...
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bioRxiv - Microbiology 2024Quote: Plasmid purification from individual clones were done starting from 7.5 ml of overnight cultures following manufacturer’s instructions for large (> 10 kb) vector (Quiaprep Spin Minipreps, Qiagen), including doubling the standard volume of reagent ...
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bioRxiv - Microbiology 2024Quote: ... for 10 min at 50 Hz in a PowerBead tube from the DNeasy PowerSoil DNA isolation kit (Qiagen) before isolating the DNA according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... 2mM EDTA) and incubated for 10min at RT with 10 mL of red blood cell lysis buffer (Qiagen). Cells were washed again with Facs buffer ...
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bioRxiv - Microbiology 2024Quote: ... dialized proteins were supplemented with 10 mM Imidazole and ran over 7.5-mL equilibrated Ni-NTA beads (Qiagen). The flowthrough was collected and concentrated using 30 kDa molecular weight cut-off centrifugal filters (Merck Millipore ...
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bioRxiv - Microbiology 2024Quote: ... dialized proteins were supplemented with 10 mM Imidazole and ran over 7.5-mL equilibrated Ni-NTA beads (Qiagen). The flowthrough was collected and and concentrated using 100 kDa molecular weight cut-off centrifugal filters (Merck Millipore ...
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNA was extracted 96h after transfection using homemade cell lysis buffer (10 mM Tris-HCl pH8.5 and 0.05% SDS) or DNeasy Blood & Tissue Kits (Qiagen) to evaluate editing frequencies ...
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bioRxiv - Genomics 2024Quote: ... freemani beetles (10 pooled male and female individuals) using the DNeasy Blood and Tissue Kit (Qiagen, Hilden, Germany). The isolated genomic DNA was quantified with Qubit 2.0 DNA HS Assay (ThermoFisher ...
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bioRxiv - Bioengineering 2024Quote: ... with ∼5-10 mg of each organ homogenized into 300 μL homogenization buffer using stainless steel beads (Qiagen) in a TissueLyser II tissue homogenizer (Qiagen ...
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bioRxiv - Genetics 2024Quote: ... about 10% of the cells were collected and DNA extracted using the DNeasy Blood and Tissue Kit (Qiagen) using the manufacturer’s recommended protocol ...
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bioRxiv - Developmental Biology 2024Quote: DNA was extracted from 10 mg of dissected E14.5 tissue samples using the DNeasy blood & tissue kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... DNA extraction for sediment samples was carried out using DNeasy PowerMax Soil DNA Extraction Kit (QIAGEN 12988-10, Germany) according to the manufacturer’s instruction ...
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bioRxiv - Genomics 2020Quote: ... 0.4 M NaOH and 10 mM Tris-acetated (pH 7.6) and then hybridized to the sequencing primer in PyroMark annealing buffer (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... while method-3 was a modified protocol of the DNeasy PowerMax Soil Kit (Cat.No. 12988-10) provided by Qiagen (based on communication exchanged with the manufacturer) ...
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bioRxiv - Plant Biology 2020Quote: ... Each sample was normalized to contain 10 ng/μl DNA using QIAgility Liquid Handling System (Qiagen, Germantown, Maryland, USA) for library construction ...
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bioRxiv - Cell Biology 2021Quote: RNA from 5-10 million NK cells treated overnight with indicated conditions was extracted using Rneasy Plus kit (Qiagen). Samples were prepared for Illumina sequencing following the manufacturer’s protocol using the TruSeq® stranded total RNA with rRNA depletion using Ribo Zero TM Human/Mouse/Rat ...
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bioRxiv - Cell Biology 2021Quote: Tissue lysates were further homogenized and centrifuged at 10,000 g for 10 min at 4°C in microcentrifuge spin columns (QIAshredder, Qiagen) to obtain clear protein lysate ...
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bioRxiv - Microbiology 2021Quote: ... The centrifugation step was repeated and the pellet air-dried before the DNA was dissolved in 10 mM Tris-HCl (pH 8.5, Qiagen) at 65 °C for 20 min ...
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bioRxiv - Systems Biology 2020Quote: ... The sealed plates were subjected to 10 minutes of bead beating at 20 Hz using an (Qiagen Tissuelyser II). After bead beating ...
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bioRxiv - Microbiology 2020Quote: ... Crystals of Brd4(BD1) with YFV-C (1-10) K4ac peptide were grown from a solution containing 0.2 M sodium chloride (QIAGEN), 0.1 M Tris (pH 7.0 ...
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bioRxiv - Cell Biology 2021Quote: RNA was extracted from platelet pellets (10 million per population) using the RNeasy Mini kit according to manufacturer’s instructions (Qiagen). cDNA was synthesized from extracted platelet RNA using SuperScript® III first-strand synthesis system for RT-PCR (Life Technologies) ...
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bioRxiv - Biochemistry 2022Quote: ... 10% glycerol and 1 mM DTT) and recombinant TrcP was purified from clarified lysates using Ni-NTA resin (Qiagen) and gravity chromatography ...
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bioRxiv - Molecular Biology 2022Quote: His-Prs or its variants (10 µg) were bound to 4 µl of Ni-NTA Magnetic Agarose Beads (Qiagen) in 50 µl interaction buffer (50 mM Tris pH 9.0 ...
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bioRxiv - Molecular Biology 2022Quote: ... Embryos and the surrounding maternal calyptra tissue were dissected out of the archegoniophore into 10% RNALater (Qiagen, Hilden, Germany) on Microscope slides with cavities (Marienfeld Superior ...