Labshake search
Citations for Qiagen :
4101 - 4150 of 10000+ citations for Mouse Marginal Zone B And B1 Cell Specific Protein MZB1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... a pool of 100 million cells from each screen was harvested for genomic DNA (gDNA) extraction using the QIAamp DNA Blood Maxi Kit (QIAGEN).
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bioRxiv - Microbiology 2023Quote: ... an unbiased amplification method was employed by amplifying the purified DNA at 30°C for 8 hours using a REPLI-g Single Cell kit (Qiagen). The amplified DNA was purified using AMPure XP magnetic beads (Beckman Coulter ...
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bioRxiv - Pathology 2023Quote: Total RNA preparation was performed from 20000 sorted cells (macrophage subsets or adipose tissue CD9hi progenitors) using the RNeasy Plus Micro Kit (Qiagen). cDNA libraries were generated using the KAPA mRNA HyperPrep kit (Roche) ...
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bioRxiv - Cancer Biology 2023Quote: ... The ligation reaction was used to transform commercial MC1061F cells by electroporation and purified using the QIAprep Spin Miniprep Kit (Qiagen).
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bioRxiv - Cancer Biology 2023Quote: ... A DNA fragment containing 248nt of the NF1 coding region of interest was PCR-amplified from single-strand cDNA obtained from parental and clonal cells using QIAquick Gel Extraction Kit (QIAGEN). Incorporation was confirmed by Sanger sequencing the amplicon ...
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bioRxiv - Genetics 2023Quote: Genomic DNA of subclones from a 12-well plate along with 2 million parental cells were extracted using DNeasy Blood & Tissue Kit (QIAGEN) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Cells were harvested directly in Buffer RLT with β-mercaptoethanol and RNA processed directly using the RNeasy Mini Kit (Qiagen), with on-column DNase digestion according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... GFP+ (recombined) podocytes were sorted by FACS from dissociated kidneys and isolated cells immediately processed with RNeasy micro kit (Qiagen) to obtain purified total RNA ...
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bioRxiv - Genomics 2023Quote: ... Genomic DNA was extracted from the 40 red blood cell samples using a QIAGEN Blood and Tissue Kit (Qiagen, Germany), following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... cells from the blood of various donors were used to prepare genomic DNA with the DNeasy Blood & Tissue Kit (Qiagen). Approximately 50 ng genomic double-stranded DNA per donor was converted with sodium bisulfite using the EZ DNA Methylation-Lightning Kit (Zymo Research ...
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bioRxiv - Immunology 2023Quote: ... Immune cells were FACS-sorted for APC-enriched samples or T cell-enriched samples and RNA was subsequently extracted (RNeasy Mini Kit, Qiagen). RNA was analyzed on an nCounter MAX Analysis System (Nanostring ...
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bioRxiv - Genomics 2023Quote: ... genomic DNA and total RNA were purified from the infected cells using an AllPrep DNA/RNA mini kit (Qiagen, 80204). 120 μg RNA was treated with Turbo DNase (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: Total RNA was harvested from 2-3 million HIV-dreGFP infected Jurkat cells exposed to EPZ-719 (500nM) or control (DMSO) using a RNEasy kit (Qiagen). RNA quantity and quality were then analyzed by nanodrop and Tapestation (Agilent ...
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bioRxiv - Microbiology 2023Quote: ... Cells were sorted directly into 2-Mercaptoethanol-containing RLT buffer and RNA was extracted using a RNeasy Mini kit (Qiagen). The cell populations used for RNA sequencing are summarized in Table S1 ...
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bioRxiv - Microbiology 2023Quote: RNA was harvested from infected cells 13 hours post-infection using the RNeasy Plus Mini Kit (Qiagen, Cat. No. 74134). We made a master mix of RLT lysis buffer with 1% β-mercaptoethanol to inhibit RNases ...
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bioRxiv - Microbiology 2023Quote: ... round 1 and 3 virus infections of GeCKO-A549 cells using the midi gDNA extraction kit (Qiagen, Germantown, MD, USA). The sgRNA’s DNA copies were PCR amplified from the extracted gDNAs for next generation sequencing (Fig 1) ...
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bioRxiv - Cancer Biology 2023Quote: ... of 5 million AMO1 shIRE1 cells previously treated per condition was isolated using the AllPrep DNA/RNA Mini Kit (Qiagen). gDNA was quantified using Nanodrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Patient cell-free DNA was extracted from 1ml-1.2ml of patient plasma using the QIAamp Circulating Nucleic Acid Kit (QIAGEN # 55114) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: RNA was harvested from melanoma cells 48 hours after siRNA or overexpression vector transfection by using RNeasy Plus mini kit (Qiagen) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... RNA libraries were produced from the 20 primary spermatocyte cysts using the QIAseq FX Single Cell RNA Library Kit (Qiagen). Library quality and fragment size was assessed with the D1000 Tapestation (Agilent ...
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bioRxiv - Cancer Biology 2023Quote: Genomic DNA isolation from primary GBM tumors or patient-derived GBM cells was carried out using QIAamp DNA Micro kit (Qiagen). The Illumina Infinium MethylationEPIC kit was used to analyze the DNA methylation status at >850000 5’CpG islands per sample according to the manufactureŕs instructions ...
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A CRISPR activation screen identifies FBXO22 as an E3 ligase supporting targeted protein degradationbioRxiv - Biochemistry 2023Quote: HEK293T CRISPR transcriptional activation cells after sgRNA transduction were collected and total RNAs were extracted using the RNeasy mini kit (Qiagen). cDNAs were obtained by reverse transcription using the iScript cDNA kits (Bio-Rad) ...
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bioRxiv - Cell Biology 2023Quote: ... approximately 20,000 DAPI-negative Tomato+ or YFP+ cells from dissociated tissues were sorted directly to RLT buffer (RNAeasy Micro kit – Qiagen), and RNA was extracted according to the manufacturer’s instructions and stored at –80°C ...
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bioRxiv - Systems Biology 2023Quote: Total RNA was isolated from parental MDA-MB-453 and long-term BLU9931 MDA-MB-453 cells using a RNeasy mini kit (Qiagen) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... Each adhesive cap at an density of approximately 13–60 cells was independently processed for DNA extraction using a QIAamp DNA micro kit (Qiagen). This approach allowed enough DNA input material to prepare sequencing libraries and detect variants unique to each zygote ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were decrosslinked and treated with proteinase K (0.1 mg/ml) overnight followed by genomic DNA extraction with QIAGEN Blood & Cell Culture DNA Maxi Kit (QIAGEN; 13362) and quantified by Qubit (Thermo-Scientific ...
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bioRxiv - Genetics 2024Quote: ... yeast plasmid DNA was extracted from 5x107 cells harvested off of galactose and final glucose plates of replicates 2 and 3 using Qiaprep Spin Miniprep Kit (QIAGEN). We amplified the 200-bp repair template region from the initial E ...
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bioRxiv - Cancer Biology 2024Quote: Viably frozen single-cell suspensions of primary KS skin biopsy samples were thawed and DNA was extracted from isolated cells using DNeasy kit (Qiagen). Then ...
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bioRxiv - Immunology 2024Quote: Total RNA has been extracted from cell lysates by using the RNeasy Plus Mini Kit (cat. 74136, Qiagen, Hilden, Germany). The total cDNA was obtained by reverse transcription using random primers from Invitrogen (ref ...
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bioRxiv - Cancer Biology 2024Quote: ... A total of 18 samples for each cell line were lysed in RLT buffer from RNeasy mini kit (#74106, Qiagen) and RNAs were extracted following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2024Quote: ... to a density of 2 to 3 million cells /ml and then transfected with the MP-20 containing plasmid purified using a plasmid purification Giga kit (Qiagen). PEI MAX 40k (Polysciences ...
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bioRxiv - Cancer Biology 2024Quote: Up to 2.106 T-ALL#1 GFP+ cells were sorted from femurs of NECA or vehicle-treated T-ALL mice using the Influx cell sorter and mRNA was extracted directly after sorting using the RNeasy Plus Mini kit (74134, QIAGEN). The quality of the RNA was acquired using the RNA 6000 Pico Labchip Kit (5067-1513 ...
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bioRxiv - Cancer Biology 2024Quote: ... Final samples of 3 or 30 million cells were collected and genomic DNA was extracted (DNeasy blood and Tissue kit, Qiagen). Next ...
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bioRxiv - Cancer Biology 2024Quote: ... and A498 cells infected with lentivirus expressing shRNA targeting the indicated transcripts was isolated using RNeasy Mini Kit (QIAGEN #74104) and QIAshredder (QIAGEN #79654) ...
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bioRxiv - Cancer Biology 2024Quote: ... Total RNA from MCF-7 cells treated as indicated was isolated using the Qiagen RNeasy Plus Mini kit (Qiagen, 74136) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: The EGFP-positive and EGFP-negative subpopulations of MDA-MB-231 cells were lysed using RNeasy Plus Mini Kit (74134; QIAGEN) and kept at −80°C until needed ...
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bioRxiv - Biochemistry 2024Quote: Genomic DNA from PBMCs and sorted memory CD8+ T cell subpopulations was extracted using DNeasy Blood and Tissue Kit (Qiagen) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was isolated from myometrial tissue or cultured hTERT-HM-ERα cells using an RNeasy Plus Mini Kit (Qiagen), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Viral DNA was extracted directly from 200 µL aliquots of infected cell culture and nuclease treated using a commercial QIAamp DNA Kit (Qiagen) according to the manufacturer’s procedures ...
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bioRxiv - Molecular Biology 2024Quote: ... and HET(2)) and four biological replicates of PA-1 (WT and HET) cells was extracted using miRNeasy Mini Kit (Qiagen) followed by on-column DNAse digestion ...
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bioRxiv - Microbiology 2024Quote: CD11b+ cells from brains were sorted using magnetic beads (Miltenyi Biotech) and RNA was extracted using an RNeasy micro kit (Qiagen) per the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: Total RNA was extracted from endothelial cells after stimulation with ImP (100 nM) or control for 12 h using the RNeasy Mini Kit (Qiagen) according to manufacturer instructions ...
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bioRxiv - Immunology 2024Quote: RNA was isolated from 5×106 sorted WSU-FSCCL IgG+ and IgM+ cells using the RNeasy mini kit (Qiagen, 74004). RNA quality control was performed using a Bioanalyzer (Agilent) ...
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bioRxiv - Cell Biology 2024Quote: RNA was isolated from ciDKO podocytes (3 days after Cre lentiviral transduction) and wild-type control cells (n=4 for each group) using an RNeasy mini kit (Qiagen). RNA was quantified using a Qubit Fluorimeter (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... and 48 hrs post-exposure to stress or inflammatory compounds neuronal cells were washed three times and harvested using AllPrep DNA/RNA mini kit (Qiagen) and the libraries prepped as previously described ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were collected from plates on the day noted and RNA was purified using the RNeasy mini prep kit (Qiagen), following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: Genomic DNA samples were prepared from heparinized peripheral blood or 1×106 Jurkat cells following the instructions of the QIAamp DNA Blood Mini kit (#51104; QIAGEN). Primers were designed with the help of Oligo Primer Analysis Software version 7 (Molecular Biology Insights) ...
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bioRxiv - Bioengineering 2024Quote: RNA was extracted from the cells after dynamic culture by removing the medium from the channels and adding RLT buffer (RNeasy Kit, Qiagen) for cell lysis and consequent RNA isolation ...
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bioRxiv - Genomics 2024Quote: Total RNA was isolated from 5 × 106 cells harvested from frozen patient vials using the RNeasy Plus mini-Kit (Qiagen). Libraries were prepared with the NEBNext® rRNA Depletion Kit and were sequenced using the Illumina NextSeq500 platform ...
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bioRxiv - Molecular Biology 2021Quote: ... The kits for RNA extraction were from Qiagen (RNeasy Mini Kit and RNeasy Micro Kit). The reverse transcription kit ...