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Citations for Qiagen :
4251 - 4300 of 10000+ citations for Mouse Marginal Zone B And B1 Cell Specific Protein MZB1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmid DNA extractions were performed on cell pellets from 15 mL of these back-diluted cultures using QIAprep Spin Miniprep Kit (Qiagen, 27104).
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bioRxiv - Molecular Biology 2024Quote: Total RNA was isolated from Allprotect segments of the intestine or sorted cells using the RNeasy Mini Kit (Qiagen, Valencia, CA) and reverse transcribed using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... HCoV-infected cells were examined under ZOE Cell Imager for the CPE formation followed by total vRNA extraction (QIAamp vRNA Mini kit (QIAGEN # 52904) from culture supernatants and cell lysates along with uninfected cells (control mock ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... gDNA containing RNA–DNA hybrids was isolated from HeLa cells transfected with a pFlag-IN codon-optimized plasmid using a QIAmp DNA Mini Kit (Qiagen, 51304). gDNA was sonicated for 10 min (Diagenode Bioruptor ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was extracted from the pelleted cells using the QIAgen DNeasy Blood and Tissue Kit protocol and QIAgen Qiacube (QIAGEN, Germany). The DNA concentration of extracts were measured ...
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bioRxiv - Cancer Biology 2024Quote: RNA was extracted from OSU-CLL cells (1.5e6 cells/mL) treated with DMSO or SpiD3 (1, 2 μM; 4 h) using the miRNeasy Mini Kit (Qiagen; Hilden, Germany) per manufacturer instructions and processed using the Universal Plus mRNA-Seq with NuQuant kit (Tecan ...
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bioRxiv - Cancer Biology 2023Quote: The resulting inducible cell lines were verified to be correct by extracting genomic DNA (QIAmp DNA Blood Mini Kit Qiagen #51106) and sequencing through the S284 mutation site (Eurofins) ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA from untreated and treated MDA-MB-231 cells was extracted using a RNeasy Mini Kit (Qiagen, Germantown, MD, USA). RNA sequencing was performed on the NextSeq 500 sequencing platform (Illumina ...
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bioRxiv - Cancer Biology 2023Quote: RT PCR: Total RNA was eluted from knockdown and control cells using the RNeasy® Plus mini kit (Qiagen, Ref # 74134). 2 µg of total RNA was reverse transcribed using High Capacity cDNA Reverse Transcription Kit (AppliedBiosystems ...
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bioRxiv - Cell Biology 2023Quote: ... Cell pellets were thawed and resuspended in freshly prepared RLT buffer (350 μL per sample for 1E6 cells) from the RNAeasy Qiagen kit (Qiagen 74104). Dnase1 digestion buffer was then added and cells were subsequently lysed via passage through a Qiashredder column (Qiagen 79654) ...
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bioRxiv - Systems Biology 2024Quote: ... RH30 and HepG2 cells were treated as indicated in the figures before the isolation of RNA using the RNeasy Mini Kit (Qiagen, 74104) according to the manufacturer’s instructions ...
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... Total RNA was extracted from fresh frozen cell pellet samples using Qiagen RNeasy Plus Universal mini kit following manufacturer’s instructions (Qiagen, Hilden, Germany). RNA samples were quantified using Qubit 2.0 Fluorometer (Life Technologies ...
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bioRxiv - Immunology 2024Quote: DNA and RNA were extracted from the cSCC cell lines using the DNeasy Blood and Tissue Kit (QIAGEN Cat. No. 69504) and RNeasy Mini kit (QIAGEN Cat ...
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bioRxiv - Microbiology 2024Quote: Total microbial DNA from biofilms and planktonic cells of dental plaque cultures was extracted using DNeasy PowerSoil kit (Qiagen; Germantown, MD). After the recommended quality control procedures ...
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bioRxiv - Biochemistry 2024Quote: Total RNA was isolated from cells grown in six-well plates at 70–80% confluency with an RNA extraction kit (Qiagen #74104). cDNA was synthesized using a sensiFAST cDNA synthesis kit (Meridian Bioscience #BIO-65053) ...
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bioRxiv - Cancer Biology 2024Quote: Genomic DNA was prepared from flushed marrow of cell line-injected tibiae using DNeasy Blood & Tissue Kit (#69504, Qiagen, Hilden, Germany,). A non-injected mouse bone marrow was used as a negative control ...
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bioRxiv - Cancer Biology 2023Quote: ... Germline genomic DNA was purified from cryopreserved peripheral blood mononuclear cells (PBMCs) using the Qiagen QiaAMP DNA Mini Kit (Qiaggen, 51304) according to the manufacturer’s instructions (Qiagen, Valencia, CA). The DNA quantity was assessed by fluorometry using the Qubit dsDNA HS Assay (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: DNA was extracted from 1×10^6 CD4 Th cells/ replicate using the Qiagen DNA mini kit according to manufacturer’s instructions (Qiagen Hilden, Germany). DNA was extracted from 10ng of mouse brain tissue as previously described13 using NEB Monarch High molecular weight DNA extraction kit according to manufacturer’s instructions (NEB Cat #T3050).
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bioRxiv - Immunology 2023Quote: Total RNA was isolated from a tumor-reactive T cell culture using a Qiagen RNeasy Micro kit (Qiagen GmbH, Hilden, Germany). 1μg total RNA was used for reverse transcription with the NEB Template Switching Reverse Transcriptase Enzyme Mix (New England Biolabs ...
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bioRxiv - Bioengineering 2023Quote: ... total RNA was isolated from Hep3B cell spheroids on the pillar plate and in the ULA plate using a RNeasy Plus kit (Qiagen,74134). One μg of RNA was converted into cDNA using the high-capacity cDNA reverse transcription kit according to the manufacturer instructions (Applied Biosystems ...
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bioRxiv - Cell Biology 2023Quote: ... 200μl chow fed liver lysate or 300μl WD-fed liver lysate was transferred to a 15ml tube for genomic DNA extraction using the Blood & Cell Culture DNA Midi Kit (Qiagen #13343) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: Total RNA was extracted from MCF10AER/vSrc cells (Control and treated with 10 nM D-Alanine for 4 h or 24 h) using RNeasy Kit (Qiagen, #74104) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA was extracted from fresh frozen cell pellets using Qiagen RNeasy Plus Universal mini kit following manufacturer’s instructions (Qiagen, Hilden, Germany). Extracted RNA samples were treated with TURBO DNase (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... and miRNAs from each HTM cells was reverse transcribed into cDNA using miScript II RT kit as per the manufacturer’s instruction (Qiagen, Hilden, Germany). RT-qPCR was performed in a total volume of 20 µl containing 25 ng of total cDNA ...
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TIPRL1 and its ATM-dependent phosphorylation promote radiotherapy resistance in head and neck cancerbioRxiv - Cancer Biology 2023Quote: ... Colonies were transferred to 96-well plates and grown until a sufficient number of cells was reached for genomic DNA (DNeasy Blood & Tissue Kit; Qiagen, #69504) and protein extraction ...
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bioRxiv - Genomics 2023Quote: ... About two million cells from each sample were used to extract total RNA with the RNeasy Plus Mini Kit (Qiagen, #74134), and 1 µg of RNA was used to generate cDNA with the iScript cDNA Synthesis Kit (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... Media was aspirated and the cell pellet was re-suspended in 350 μL RLT buffer (Qiagen RNeasy Mini Kit, No. 74104). Extraction was automated using a QIAcube set to the RNA-RNeasy mini protocol with DNaseI digest and 35 μL DEPC water elution ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was isolated from cell pellets in batches of 6 using an RNeasy Plus Mini Kit (Cat # 74134, Qiagen, Hilden, Germany). Acceptable RNA concentration and quality were verified with Nanodrop and Bioanalyzer measurements ...
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bioRxiv - Genomics 2023Quote: ... Total DNA was extracted from these cells following centrifugation and resuspension in 200 uL DPBS using the QIAamp DNA Mini Kit (Qiagen, 51304). 600 ng DNA were recovered ...
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bioRxiv - Genomics 2023Quote: ... These cells were used as the starting material for an RNA extraction using the RNeasy Mini Kit (Qiagen, Cat no: 74104). The final elution was collected in 30 μl of nuclease-free water ...
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bioRxiv - Neuroscience 2023Quote: ... The cell pool was cultured to produce a CRISPR-pool stock and allow genomic DNA extraction (DNeasy kit, Qiagen, Cat#69504). Successful CRISPR editing was visualized by PCR amplification of the target region (primers listed in Table S1) ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was extracted from wild type and RFX2 -/- LUHMES cells and column-purified using an RNeasy mini kit (Qiagen 74104) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... The cells containing the desired plasmids were amplified by broth culture and the plasmids were prepared by maxiprep kit (Qiagen, 12663). The plasmids were stored at −20°C until use.
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bioRxiv - Cell Biology 2023Quote: Total RNA was extracted from isolated male germ cells or whole testes using the RNeasy extraction kit (74104, Qiagen, Hilden Germany) as described above ...
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bioRxiv - Cancer Biology 2023Quote: Total genomic DNA from the sorted CD8+ T cells in the indicated groups were extracted using QIAamp DNA Micro Kit (Qiagen, 56304), eluted in a final volume of 50µL buffer AE and submitted for amplification and sequencing of the TCRβ region ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The remaining cells were lysed and genomic DNA was extracted using the QIAamp DNA Blood Mini Kit (Qiagen, Venlo, The Netherlands) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... A 1.5ml aliquot of Stbl2 cells from overnight culture was used for miniprep plasmid extraction (QIAGEN Plasmid mini kit, Cat. 12123), to check the EEV sequence ...
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bioRxiv - Bioengineering 2023Quote: ... DNA was extracted in pools of 6-8 adult mosquitoes mosquitoes using Blood & Cell Culture DNA Midi Kit (Qiagen, Cat# 13343) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: MiR libraries were generated from 100 -150 ng of cell using the QIAseq miRNA Library Kit according to the protocol (Qiagen, Germany). Library quality control and concentrations were determined using a High Sensitivity D1000 ScreenTape with TapeStation Analysis Software A.02.02 (Agilent Technologies ...
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bioRxiv - Cancer Biology 2023Quote: RNA was extracted and purified from flash frozen tumors/normal tissues and cells using a Qiagen RNeasy kit following the manufacturer’s instructions for fibrous tissue (Qiagen, Hilden, Germany). High quality RNA (RIN >7 ...
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bioRxiv - Neuroscience 2023Quote: ... cells were lysed using RLT buffer mastermix (10 uL beta-mercaptoethanol : 1 mL RLT) and cells were lysed with RNAeasy micro kit (Qiagen, 74004). RNA quality and quantity was validated using a nanodrop (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... as per the manufacturer’s instructions of the DNeasy kit using the protocol for ‘Purification of Total DNA from Animal blood or cells (Spin-Column Protocol)’ (Qiagen, Germany). DNA qPCR was performed using the primer pair targeting16 S rRNA gene (Forward-5’GCGTAGGCGGACATGTAAGT-3’ Reverse-5’AATCCCGTTCACTACCCACG-3’ ...
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bioRxiv - Microbiology 2023Quote: ... The capsules were then suspended in Buffer D2 and subjected to multiple displacement amplification (MDA) using REPLI-g Single Cell Kit (QIAGEN, Germany). Following MDA treatment at 30°C for 3 h ...
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bioRxiv - Bioengineering 2023Quote: ... Genomic DNA was extracted from 10 ♂’s and ♀’s using Blood & Cell Culture DNA Midi Kit (Qiagen, Cat# 13343) and the sequencing library was prepared using the Oxford Nanopore SQK-LSK110 genomic DNA by ligation kit ...
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bioRxiv - Biochemistry 2023Quote: ... the supernatant was aspirated from each well and the total RNA was then extracted from the cells using RNeasy mini kit (Qiagen, 74104). The total RNAs were quantified by ultraviolet absorption at 260 nm (Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from freshly sorted 30,000 PDGFRα+ cells from LA/BC muscles using the RNeasy Plus Micro Kit (Qiagen, Cat# 74034) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: Total RNA of the supernatants collected from SARS-CoV-2 infected Calu-3 cells 48 h post-infection were isolated using the QIAamp Viral RNA Mini Kit (Qiagen; #52906) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... was harvested from lung and spleen tissues and CD4+/CD8+ T cells using the Qiagen DNeasy Blood & Tissue kit (Qiagen, #69506) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... 0.4% glucose medium at 28 °C without shaking using the Blood and Cell Culture DNA Maxi Kit (Qiagen, catalog number: 13362) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... we extracted high-molecular weight DNA from fresh whole blood samples (immediately flash frozen) of the two F1 hybrid mice using a Blood and Cell Culture Mini Kit (Qiagen, GmbH) following the manufacturer’s protocol ...